Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,334,703 A→G 7.2% intergenic (+22/‑206) ispB → / → sfsB octaprenyl diphosphate synthase/malPQ operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,334,7030AG 7.2% 637.0 / 27.5 208intergenic (+22/‑206)ispB/sfsBoctaprenyl diphosphate synthase/malPQ operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (93/95);  new base G (8/7);  total (104/104)
Fisher's exact test for biased strand distribution p-value = 7.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-02

Only 100 of 208 total aligned reads displayed.

ATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCTGAATGAAAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGC  >  NC_000913/3334439‑3334960
                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                  
acagagtcgggCACAACAGAACCGGCACAGATGATCCGGAGCGGCATCGAACAGGGTAACGGGCTGCATCGTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGAGTGCTTGAATGGACGCGTCAGCGGGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTGACAGGGGCTCCCGGACACCCCGTGGCGAGAAGCACTCATCGGCGTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGa                                                                                                                                                                                                                                                         <  4:399295/266‑1 (MQ=255)
      catGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTCCAGGTGCTCCCGGACGCCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACCTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCCCAGCGCGCGGGCTGGTTc                                                                                                                                                                                                                                                   >  2:485803/1‑275 (MQ=255)
        tGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATGCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCCGTaa                                                                                                                                                                                                                                             >  1:59531/1‑279 (MQ=255)
                          acggatgatCCGTACCGCCGTCGAACAGGGTACCGGTCGCCAGCTTCTGGAACCGTGTCTGTAAGCAATGGACGCTTGTGGAGCTTTTGAATGGACGCGTCAGCGTGCCGAGGCAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTGGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCGGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAac                                                                                                                                                                                                                                <  4:22063/271‑1 (MQ=255)
                             gatgatCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCACCCCGCGGGATGGTTCCCGTAAGTTCCATAAAAacccct                                                                                                                                                                                                                            >  4:261247/1‑271 (MQ=255)
                              atgatCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATaaa                                                                                                                                                                                                                                   <  4:485555/267‑1 (MQ=255)
                                                           cGGTTGTCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGGGGATCGCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg                                                                                                                                                                                              <  2:542533/275‑1 (MQ=255)
                                                               cGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAg                                                                                                                                                                                                                      <  2:312990/247‑1 (MQ=255)
                                                                gCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgtg                                                                                                                                                                                         <  2:594867/275‑1 (MQ=255)
                                                                gCCATCTTCTGCAACCCGTTCTGGAAGGCATTAACGATTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGACTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgtg                                                                                                                                                                                         <  2:723967/275‑1 (MQ=255)
                                                                     cttttGGAAACGGTTCTGGAATCAATGAACGCATATGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATc                                                                                                                                                                                     <  2:386520/274‑1 (MQ=255)
                                                                                   tCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGaa                                                                                                                                                                      <  2:555961/275‑1 (MQ=255)
                                                                                                          gATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTcac                                                                                                                                                               <  2:243078/259‑1 (MQ=255)
                                                                                                                ttGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTaa                                                                                                                                                                                                                                             >  2:700074/1‑175 (MQ=255)
                                                                                                                    aTGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTc                                                                                                                                                                                                                                         <  4:700074/175‑1 (MQ=255)
                                                                                                                          gcgTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAgcgcg                                                                                                                                                                                                                                                              >  8:471896/1‑148 (MQ=255)
                                                                                                                            gTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGcgcgcg                                                                                                                                                                                                                                                            <  7:496230/148‑1 (MQ=255)
                                                                                                                             tCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCgg                                                                                                                                                                                                                                                           >  8:36718/1‑148 (MQ=255)
                                                                                                                                 cGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATgg                                                                                                                                                                                                                                                      <  6:506702/149‑1 (MQ=255)
                                                                                                                                   tGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGCGCTGCGCGGGATgagg                                                                                                                                                                                                                                                    <  5:706950/149‑4 (MQ=255)
                                                                                                                                   tGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGCGCTGCGCGGGATgagg                                                                                                                                                                                                                                                    <  6:270828/149‑4 (MQ=255)
                                                                                                                                      cGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGTACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCa                                                                                                                                                                                                                                                 <  5:311467/149‑1 (MQ=255)
                                                                                                                                       gAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAg                                                                                                                                                                                                                                                >  5:901322/1‑149 (MQ=255)
                                                                                                                                        aGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAg                                                                                                                                                                                                                                                >  8:834724/1‑148 (MQ=255)
                                                                                                                                          gaagaaGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGTGCGCGGGATGGTTCCAGTa                                                                                                                                                                                                                                              <  7:359335/148‑1 (MQ=255)
                                                                                                                                           aagaagCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTaa                                                                                                                                                                                                                                             <  8:878139/148‑1 (MQ=255)
                                                                                                                                            agaagCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCTCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGt                                                                                                                                                                                                                                           >  6:291550/1‑149 (MQ=255)
                                                                                                                                               agCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCTGCGCGGGa                                                                                                                                                                                                                                                         <  8:309939/132‑1 (MQ=255)
                                                                                                                                               agCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCTGCGCGGGa                                                                                                                                                                                                                                                         >  7:309939/1‑132 (MQ=255)
                                                                                                                                                gCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCa                                                                                                                                                                                                                                       >  6:806923/1‑149 (MQ=255)
                                                                                                                                                gCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCa                                                                                                                                                                                                                                       >  5:488448/1‑149 (MQ=255)
                                                                                                                                                  aGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATa                                                                                                                                                                                                                                     >  5:133570/1‑149 (MQ=255)
                                                                                                                                                      aatgcccgcGCAGCGTTACAGGTGCTCCCGGGACACCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCGGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGATGCTCGACag                                                                                                    <  4:658523/266‑1 (MQ=255)
                                                                                                                                                          ccATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAacac                                                                                                                                                                                                                              >  8:515797/1‑148 (MQ=255)
                                                                                                                                                          ccATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACt                                                                                                                                                                                                                             >  6:722959/1‑149 (MQ=255)
                                                                                                                                                              cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCGTAAAAACACTTAtt                                                                                                                                                                                                                         <  5:806923/149‑1 (MQ=255)
                                                                                                                                                              cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTAt                                                                                                                                                                                                                          <  7:118918/148‑1 (MQ=255)
                                                                                                                                                              cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTg                                                                                                                                                                                                        <  3:40982/166‑1 (MQ=255)
                                                                                                                                                              cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTg                                                                                                                                                                                                        >  1:40982/1‑166 (MQ=255)
                                                                                                                                                                         aGGTGCTCCCGGACACCCCTTGGCGTGAAGCACTCATCGGCCGCGAGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGGAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTAGTCAGctctgcaa                                                                                                                                                                                                              <  6:287090/149‑5 (MQ=255)
                                                                                                                                                                             gCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCTGAATGAAAGCGa                                                                            <  4:560127/275‑1 (MQ=255)
                                                                                                                                                                              cTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCt                                                                                                                                                                                                         <  6:14337/149‑1 (MQ=255)
                                                                                                                                                                               tCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTg                                                                                                                                                                                                        >  5:560281/1‑149 (MQ=255)
                                                                                                                                                                                cccGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTg                                                                                                                                                                                                        >  7:378836/1‑148 (MQ=255)
                                                                                                                                                                                cccGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGa                                                                                                                                                                                                       >  6:98040/1‑149 (MQ=255)
                                                                                                                                                                                    ggACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgtg                                                                                                                                                                                         <  2:624211/160‑1 (MQ=255)
                                                                                                                                                                                         ccccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAgg                                                                                                                                                                                               <  6:901322/149‑1 (MQ=255)
                                                                                                                                                                                         ccccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGCGCTGCGCGGGATg                                                                                                                                                                                                                                                       >  7:452617/1‑93 (MQ=39)
                                                                                                                                                                                          cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa                                                                                                                                                                                                 <  5:640646/146‑1 (MQ=255)
                                                                                                                                                                                          cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa                                                                                                                                                                                                 >  6:640646/1‑146 (MQ=255)
                                                                                                                                                                                           ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATaa                                                                                                                                                                                                              >  7:435576/1‑132 (MQ=255)
                                                                                                                                                                                           ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg                                                                                                                                                                                              <  8:435903/148‑1 (MQ=255)
                                                                                                                                                                                           ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGa                                                                                                                                                                                             <  5:793653/149‑1 (MQ=255)
                                                                                                                                                                                                gCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgtg                                                                                                                                                                                         >  7:258727/1‑148 (MQ=255)
                                                                                                                                                                                                   agaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCAt                                                                                                                                                                                      >  7:607222/1‑148 (MQ=255)
                                                                                                                                                                                                    gaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCtata                                                                                                                                                                                                               >  2:154797/1‑122 (MQ=255)
                                                                                                                                                                                                    gaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGATCTGAATGAAAGCGACTAACTTAAGGAGTGAGGaaaa                                                      >  2:458405/1‑275 (MQ=255)
                                                                                                                                                                                                     aaGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCtt                                                                                                                                                                                   >  5:890233/1‑149 (MQ=255)
                                                                                                                                                                                                          cTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtg                                                                                                                                                                                           >  8:273444/1‑136 (MQ=255)
                                                                                                                                                                                                          cTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtatat                                                                                                                                                                              >  6:564404/1‑149 (MQ=255)
                                                                                                                                                                                                          cTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGATCTTAATGAAAGCGACTAACTTAAGGGTTGCGGCAAAATGGa                                                 >  4:154533/1‑274 (MQ=255)
                                                                                                                                                                                                           tCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATg                                                                                                                                                                                                                                                       >  7:807704/1‑75 (MQ=255)
                                                                                                                                                                                                             aTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATAtt                                                                                                                                                                           >  6:19775/1‑149 (MQ=255)
                                                                                                                                                                                                             aTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATAtt                                                                                                                                                                           <  5:512057/149‑1 (MQ=255)
                                                                                                                                                                                                             aTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATAtt                                                                                                                                                                           >  5:954547/1‑149 (MQ=255)
                                                                                                                                                                                                               cGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGtt                                                                                                                                                                                                                                                    <  7:573665/74‑1 (MQ=255)
                                                                                                                                                                                                                 gCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTg                                                                                                                                                                        >  7:1028327/1‑148 (MQ=255)
                                                                                                                                                                                                                      gcgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATAtt                                                                                                                                                                  <  5:246422/149‑1 (MQ=255)
                                                                                                                                                                                                                      gcgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATAtt                                                                                                                                                                  >  5:546069/1‑149 (MQ=255)
                                                                                                                                                                                                                        tcacaTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCTGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTca                                                                                                                                                                <  5:1051342/148‑1 (MQ=255)
                                                                                                                                                                                                                        gcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTc                                                                                                                                                                 <  7:870610/148‑1 (MQ=255)
                                                                                                                                                                                                                          acaTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa                                                                                                                                                                                                 <  6:430595/114‑1 (MQ=255)
                                                                                                                                                                                                                          acaTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa                                                                                                                                                                                                 >  5:430595/1‑114 (MQ=255)
                                                                                                                                                                                                                              cGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACActc                                                                                                                                                           <  8:10734/148‑1 (MQ=255)
                                                                                                                                                                                                                                cTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCttt                                                                                                                                                        >  5:362188/1‑149 (MQ=255)
                                                                                                                                                                                                                                cTGTTCAACGCGATCGTTAATCACCTCACCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACATGAAAAATAGGGATGTGCATCTTTATATAGTCTGAATATTCACACTCTTTCCAGGAACTTTTTAGAGAAATAGGCCATCAGGAGTATAGTGATGCGCGACAGAAGAAGGGTTATGAATGACAGCGACAAACTTAAGGAGTGAGGAAAAAGGGAAAGtaatttaattacatggactc                          >  4:605522/1‑265 (MQ=255)
                                                                                                                                                                                                                                  gTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTAc                                                                                                                                                      <  6:853978/149‑1 (MQ=255)
                                                                                                                                                                                                                                   ttCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACa                                                                                                                                                     >  6:1009612/1‑149 (MQ=255)
                                                                                                                                                                                                                                            aTCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGaa                                                                                                                                                 >  5:170250/1‑144 (MQ=255)
                                                                                                                                                                                                                                             tCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgtg                                                                                                                                                                                         >  4:379560/1‑103 (MQ=255)
                                                                                                                                                                                                                                             tCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCTGAATGAAAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGAcatcat             >  2:320874/1‑275 (MQ=255)
                                                                                                                                                                                                                                                      ccctcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATa                                                                                                                                  <  6:939939/149‑1 (MQ=255)
                                                                                                                                                                                                                                                       cctcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATa                                                                                                                                  >  8:624646/1‑148 (MQ=255)
                                                                                                                                                                                                                                                        ctcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAgg                                                                                                                                <  6:546069/149‑1 (MQ=255)
                                                                                                                                                                                                                                                         tcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgtg                                                                                                                                                                                         >  1:119154/1‑91 (MQ=255)
                                                                                                                                                                                                                                                         tcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgtg                                                                                                                                                                                         <  3:119154/91‑1 (MQ=255)
                                                                                                                                                                                                                                                         tcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAg                                                                                                                                 <  1:24522/147‑1 (MQ=255)
                                                                                                                                                                                                                                                         tcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAg                                                                                                                                 >  3:24522/1‑147 (MQ=255)
                                                                                                                                                                                                                                                         tcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCTGAATGAAAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTgc  >  2:687800/1‑274 (MQ=255)
                                                                                                                                                                                                                                                           ccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCa                                                                                                                             <  6:667993/149‑1 (MQ=255)
                                                                                                                                                                                                                                                              tcATCCCGCGCAGCGCGCGGGATTGTTCAAGTAAGTTCCATAAAAACACTTATTCATCTCTATAACCCCTGAAAAATAGGGATGTGTTTTTTTATATATTCTGAATATTCACAGTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCa                                                                                                                          <  6:321561/149‑1 (MQ=255)
                                                                                                                                                                                                                                                               cATCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGaa                                                                                                                                                                                                      >  5:1042222/1‑72 (MQ=38)
                                                                                                                                                                                                                                                               cATCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTaga                                                                                                                                        <  5:666342/134‑1 (MQ=255)
                                                                                                                                                                                                                                                                aTCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAg                                                                                                                         >  8:179060/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                aTCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAAc                                                                                                                                                >  2:768705/1‑125 (MQ=255)
                                                                                                                                                                                                                                                                 tCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATAtt                                                                                                                                                                           >  8:71556/1‑97 (MQ=255)
                                                                                                                                                                                                                                                                 tCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAgg                                                                                                                        >  8:421212/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                 tCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAgg                                                                                                                        >  7:656755/1‑148 (MQ=255)
                                                                                                                                                                                                                                                                 tCCCGCGCAGCGCTCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAgg                                                                                                                        <  8:1028327/148‑1 (MQ=255)
                                                                                                                                                                                                                                                                    cgcgCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACtttt                                                                                                                                            <  4:768705/125‑1 (MQ=255)
                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                  
ATGCATCATGGCACACCAGAACAGGCACAGATGATCCGTACCGCCATCGAACAGGGTAACGGTCGCCATCTTCTGGAACCGGTTCTGGAAGCAATGAACGCTTGTGGATCTCTTGAATGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGATGCTCGACAGAAGAAGTGTTCTGAATGAAAGCGACTAACTTAAGGAGTGAGGAAAAATGGAAAGTAATTTCATTGACTGGCATCCCGCTGACATCATTGCGGGTTTGC  >  NC_000913/3334439‑3334960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: