Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,354,537 C→G 6.3% intergenic (‑487/‑188) yhcC ← / → gltB putative Fe‑S oxidoreductase, Radical SAM superfamily protein/glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,354,5370CG 6.3% 545.3 / 22.5 160intergenic (‑487/‑188)yhcC/gltBputative Fe‑S oxidoreductase, Radical SAM superfamily protein/glutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base C (75/73);  new base G (5/5);  total (82/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.12e-01

Only 100 of 163 total aligned reads displayed.

CGTCATTACCAATTAAGGCAGTATAAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACA  >  NC_000913/3354268‑3354803
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                          
     ttACCAATTAAGGCAGTATAAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCtcat                                                                                                                                                                                                                                                                    <  4:33831/273‑1 (MQ=255)
                ggCAGTATAAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATATGCTTGCCATTTGACCTGTATCAGCTTGCCCGATAAGTTGGAATTCCGCTGGAAGCTTTCTGGATGTGCAGCCTGCCCATCATTTCTAtcc                                                                                                                                                                                                                                                         >  4:713767/1‑271 (MQ=255)
                               tGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTCAAGAACATTTCTGTACCAATAAGCTTGCAATTTGACCTGTATCAGCTTTCCNGATAATTTGGAAATCCGCTGGAAGCTTTCTTGATGAGCACACTTCGCATCATTTTTATTCAGTAATTTAAATCCCCTCttt                                                                                                                                                                                                                                    >  3:187784/1‑278 (MQ=255)
                                                                  aaCACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCCCTTCACCGTATTAACCGATGCGAAAAGGAcaacaa                                                                                                                                                                                                     >  2:429856/1‑275 (MQ=255)
                                                                                   gTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAATCTTGCCATTTGACCTGTATCAGCTTTCCCGCTAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCGGCCTGCTCATCATATTTATGCAGTAATTGAGATCCACTCTTCACCGTTTTAACCGCTGCGAAAAGGACAACCAGGTGGCGAATACGAgg                                                                                                                                                                                     >  2:230392/1‑274 (MQ=255)
                                                                                                              cTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGc                                                                                                                                                         <  4:393103/275‑1 (MQ=255)
                                                                                                                 cccTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCCGCTTTCCCGATAAGTTGGAAATCCGCTGGANGCTTTCTGGATGCGCAGACTGCTCATCATCTTACTGCAGTAATTGAGATCCCCTATTCAGCGTATTAACCTATGCGAAAANGACAACAAGGGGGGGAATGCGAGGGNGGCGTATTACAAGCACACCccgccgaccagcac                                                                                                                                                  >  3:148372/1‑271 (MQ=255)
                                                                                                                  ccTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACg                                                                                                                                                     >  4:666650/1‑275 (MQ=255)
                                                                                                                            ttAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTg                                                                                                                                                                                                                                                                         >  6:443141/1‑149 (MQ=255)
                                                                                                                            ttAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgt                                                                                                                                           >  4:132085/1‑275 (MQ=255)
                                                                                                                             tAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTg                                                                                                                                                                                                                                                                         >  8:549077/1‑148 (MQ=255)
                                                                                                                                  aTAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGGCTGCtcatc                                                                                                                                                                                                                                                                   <  5:919081/149‑1 (MQ=255)
                                                                                                                                  aTAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGGCTGCtcatc                                                                                                                                                                                                                                                                   <  5:550658/149‑1 (MQ=255)
                                                                                                                                   tAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCtcatc                                                                                                                                                                                                                                                                   <  7:756062/148‑1 (MQ=255)
                                                                                                                                      taTCCATTTTAATTTCAGTCATTTAATAATGAATGTTGCGCTAAAGCGCATTTCTTTACCGATGAGCTTGCCATGTTACCTGTATCAGCTTTCCCGATGAGTTGTAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCata                                                                                                                                                                                                                                                                <  7:36276/147‑1 (MQ=255)
                                                                                                                                        tCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGGCTg                                                                                                                                                                                                                                                                         >  7:183629/1‑137 (MQ=255)
                                                                                                                                        tCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATAttt                                                                                                                                                                                                                                                             >  6:578862/1‑149 (MQ=255)
                                                                                                                                          cATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTAt                                                                                                                                                                                                                                                           <  5:8430/149‑1 (MQ=255)
                                                                                                                                                aaTTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGt                                                                                                                                                                                                                                                      <  8:195760/148‑1 (MQ=255)
                                                                                                                                                       gTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTgaga                                                                                                                                                                                                                                              >  5:16534/1‑149 (MQ=255)
                                                                                                                                                        tCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGAt                                                                                                                                                                                                                                             <  6:817898/149‑1 (MQ=255)
                                                                                                                                                         cATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGAt                                                                                                                                                                                                                                             <  7:992380/148‑1 (MQ=255)
                                                                                                                                                            ttAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGGCTGCtcatcc                                                                                                                                                                                                                                                                  <  7:610203/124‑2 (MQ=255)
                                                                                                                                                            ttAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGGCTGCtcatcc                                                                                                                                                                                                                                                                  >  8:610203/1‑123 (MQ=255)
                                                                                                                                                                taaAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCtt                                                                                                                                                                                                                                     >  5:344519/1‑149 (MQ=255)
                                                                                                                                                                 aaaGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTc                                                                                                                                                                                                                                    >  5:112447/1‑149 (MQ=255)
                                                                                                                                                                  aaGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCa                                                                                                                                                                                                                                   >  6:489408/1‑149 (MQ=255)
                                                                                                                                                                   aaaaTTTTGCGCTAAAGCACATTTGTGTACCAATAAGCTTGCCATTTGACCTGTGTCAGCTTTCCCGATATGTTGGAAGTCCGCTGGAAGCTTTCTGGATGAGCGGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCa                                                                                                                                                                                                                                   >  8:888337/3‑148 (MQ=255)
                                                                                                                                                                           gcgcTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAATCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTggggg                                                                                            <  2:132085/275‑1 (MQ=255)
                                                                                                                                                                           gcgcTAAAGAACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGt                                                                                        <  1:187784/279‑1 (MQ=255)
                                                                                                                                                                            cgcTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAAc                                                                                                                                                                                                                         <  6:840869/149‑1 (MQ=255)
                                                                                                                                                                            cgcTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAAc                                                                                                                                                                                                                         <  6:371143/149‑1 (MQ=255)
                                                                                                                                                                              cTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCata                                                                                                                                                                                                                                                                <  7:775199/108‑1 (MQ=255)
                                                                                                                                                                                aaaGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAAtt                                                                                                                                                                                                                                                  >  6:347642/1‑120 (MQ=255)
                                                                                                                                                                                  aGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATg                                                                                                                                                                                                                    >  8:1042899/1‑148 (MQ=255)
                                                                                                                                                                                   gCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGc                                                                                                                                                                                                                   <  8:37418/148‑1 (MQ=255)
                                                                                                                                                                                       aTTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGaaaa                                                                                                                                                                                                              <  6:763389/149‑1 (MQ=255)
                                                                                                                                                                                       aTTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGaaa                                                                                                                                                                                                               <  8:837834/148‑1 (MQ=255)
                                                                                                                                                                                         ttCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGCGCAGCCTGCTCTTCATATTTATGCAGTAATTTAGATCCCCTCTTCACTGTATTAACCGATGCGAAAAGGACAACAATGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGTGGAGCGTCACGctat                                                                               >  2:52113/1‑274 (MQ=255)
                                                                                                                                                                                              tACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAg                                                                                                                                                                                                             >  6:988533/1‑143 (MQ=255)
                                                                                                                                                                                              tACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAg                                                                                                                                                                                                             <  5:988533/143‑1 (MQ=255)
                                                                                                                                                                                              tACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTa                                                                         >  2:410865/1‑275 (MQ=255)
                                                                                                                                                                                               aCCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGaca                                                                                                                                                                                                         <  2:36938/146‑1 (MQ=255)
                                                                                                                                                                                                  aaTAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTGATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGacaacagg                                                                                                                                                                                                    <  8:75236/148‑3 (MQ=255)
                                                                                                                                                                                                  aaTAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAgg                                                                                                                                                                                                   >  5:894089/1‑149 (MQ=255)
                                                                                                                                                                                                  aaTAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAg                                                                                                                                                                                                    <  7:566728/148‑1 (MQ=255)
                                                                                                                                                                                                  aaTAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAg                                                                                                                                                                                                    >  8:513937/1‑148 (MQ=255)
                                                                                                                                                                                                   aTAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGc                                                                                                                                                         >  4:329208/1‑190 (MQ=255)
                                                                                                                                                                                                   aTAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTg                                                                                                                  >  2:696306/1‑229 (MQ=255)
                                                                                                                                                                                                      aGCTTGCCATTTTACCTCTATACGCTTTCCCGATAAGTTGGAAATCCGCTTGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAAACGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAAt                                                                 <  2:713767/275‑1 (MQ=255)
                                                                                                                                                                                                       gCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCtcat                                                                                                                                                                                                                                                                    <  4:97843/79‑1 (MQ=255)
                                                                                                                                                                                                       gCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCtcat                                                                                                                                                                                                                                                                    <  2:625202/79‑1 (MQ=255)
                                                                                                                                                                                                        cTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCg                                                                                                                                                                                              <  7:718646/148‑1 (MQ=255)
                                                                                                                                                                                                           gCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTAt                                                                                                                                                                                                                                                           >  2:766821/1‑84 (MQ=255)
                                                                                                                                                                                                           gCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGGTCACGATATGTTTTACGATAAATc                                                                >  2:309224/1‑271 (MQ=255)
                                                                                                                                                                                                            ccATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATg                                                                                                                                                                                          <  8:773144/148‑1 (MQ=255)
                                                                                                                                                                                                            ccATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATg                                                                                                                                                                                          >  8:950774/1‑148 (MQ=255)
                                                                                                                                                                                                              aTTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTg                                                                                                                                                                                                                                                                         <  2:582240/67‑1 (MQ=255)
                                                                                                                                                                                                                ttGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAg                                                                                                                                                                                      <  8:771892/148‑1 (MQ=255)
                                                                                                                                                                                                                   aCCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgc                                                                                                                                                                                  >  5:308052/1‑149 (MQ=255)
                                                                                                                                                                                                                     cTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc                                                                                                                                                                                >  5:28843/1‑149 (MQ=255)
                                                                                                                                                                                                                      tGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc                                                                                                                                                                                >  8:1011504/1‑148 (MQ=255)
                                                                                                                                                                                                                       gTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTTTACGATAAATCCCTTGAGAGGGATAAc                                                >  4:605868/1‑275 (MQ=255)
                                                                                                                                                                                                                        tATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTa                                                                                                                                                                             >  6:296644/1‑149 (MQ=255)
                                                                                                                                                                                                                          tCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTga                                                                                                                                                                                                                                                >  6:215886/1‑80 (MQ=255)
                                                                                                                                                                                                                          tCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTga                                                                                                                                                                                                                                                <  5:215886/80‑1 (MQ=255)
                                                                                                                                                                                                                          tCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg                                                                                                                                                                           >  5:703630/1‑149 (MQ=255)
                                                                                                                                                                                                                           cAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGCAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg                                                                                                                                                                           <  8:648158/148‑1 (MQ=255)
                                                                                                                                                                                                                           cAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg                                                                                                                                                                           >  7:846765/1‑148 (MQ=255)
                                                                                                                                                                                                                           cAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGa                                                                                                                                                                          <  6:16534/149‑1 (MQ=255)
                                                                                                                                                                                                                           cAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGa                                                                                                                                                                          <  6:308052/149‑1 (MQ=255)
                                                                                                                                                                                                                             gCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGaca                                                                                                                                                                        <  6:942242/149‑1 (MQ=255)
                                                                                                                                                                                                                              cTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGacac                                                                                                                                                                       <  5:296644/149‑1 (MQ=255)
                                                                                                                                                                                                                              cTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt                                                                                                                                             >  4:603881/1‑175 (MQ=255)
                                                                                                                                                                                                                               tttCCCGATAAGTTCGAAATCCGCAGGAAGCTATCTGGATGAGCAGCCTCCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGCTGCGAAAAGGACAACAAGTGGGCGAATGCGAGGCGCGCGTAGGACACg                                                                                                                                                                      >  6:779359/1‑149 (MQ=255)
                                                                                                                                                                                                                                  cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATAtt                                                                                                                                                                                                                                                              >  2:169231/1‑58 (MQ=255)
                                                                                                                                                                                                                                  cccGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACTGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCAACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGACGTGGGGTTCCCGCAGACCCTGGGGGCGGTGCGCGCTATTTTGGACGATAAACACCAAGAGAGGGCAAACGGTGGTTTCgg                                     >  4:714813/1‑275 (MQ=255)
                                                                                                                                                                                                                                    cGATAAGTTGGGAATCCGCTGGAAGCTTTCGGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGATATCCCCTCTTCACCGTATTAACCGCTGCGAAAAGGACAACCAGGGGGCGAATGCGAGGCGCGCGGATGACACGCAAACCCCGTCGACACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGAt                               <  1:148372/279‑1 (MQ=255)
                                                                                                                                                                                                                                    cGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGa                                                                                                                                                                                                           >  5:101730/1‑107 (MQ=255)
                                                                                                                                                                                                                                    cGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGa                                                                                                                                                                                                           <  6:101730/107‑1 (MQ=255)
                                                                                                                                                                                                                                    cGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGATAACAAGGGGGCGAATGCGAGGCGCGCGTATg                                                                                                                                                                           >  7:137268/1‑139 (MQ=255)
                                                                                                                                                                                                                                    cGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGATAACAAGGGGGCGAATGCGAGGCGCGCGTATg                                                                                                                                                                           <  8:137268/139‑1 (MQ=255)
                                                                                                                                                                                                                                    cGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAc                                                                                                                                                                 <  6:968623/149‑1 (MQ=255)
                                                                                                                                                                                                                                     gATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAgg                                                                                                                                                                                                            <  8:25166/105‑1 (MQ=255)
                                                                                                                                                                                                                                     gATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAgg                                                                                                                                                                                                            >  7:25166/1‑105 (MQ=255)
                                                                                                                                                                                                                                      aTAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                                                                                                                                                                                                                          <  4:169231/58‑1 (MQ=255)
                                                                                                                                                                                                                                       tAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAcccc                                                                                                                                                              <  5:271625/149‑1 (MQ=255)
                                                                                                                                                                                                                                          gTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTc                                                                                                                                                           >  6:500912/1‑149 (MQ=255)
                                                                                                                                                                                                                                          gTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTc                                                                                                                                                           <  6:277292/149‑1 (MQ=255)
                                                                                                                                                                                                                                            tGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCTTCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGa                                                                                                                                                                                       >  8:498195/1‑119 (MQ=255)
                                                                                                                                                                                                                                            tGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGa                                                                                                                                                                                       <  7:498195/119‑1 (MQ=255)
                                                                                                                                                                                                                                                     gCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGaa                                                                                                                                                                                                                <  3:85953/85‑1 (MQ=255)
                                                                                                                                                                                                                                                     gCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGaa                                                                                                                                                                                                                >  1:85953/1‑85 (MQ=255)
                                                                                                                                                                                                                                                      cTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAggggg                                                                                                                                                                                                >  8:248390/1‑100 (MQ=255)
                                                                                                                                                                                                                                                            gCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgtg                                                                                                                                          <  7:182468/148‑1 (MQ=255)
                                                                                                                                                                                                                                                              tttCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGccc                                                                                                                                       >  5:592605/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                    gATGAGCAGGCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgc                                                                                                                                 >  6:268383/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     aTGAGCAGGCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCa                                                                                                                                >  5:965793/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                           agcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGtt                                                                                                                          >  5:451656/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                            gcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATAACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTc                                                                                                                         <  5:785642/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                          
CGTCATTACCAATTAAGGCAGTATAAAATGCTGGTTTTGTCGTCAGTTCAAGGCAGGATAAGGGTTAACACACCTTTATGACAGTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACA  >  NC_000913/3354268‑3354803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: