| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,991,459 | A→G | 8.3% | S103G (AGC→GGC) | cyaA → | adenylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,991,459 | 0 | A | G | 8.3% | 793.6 / 41.0 | 255 | S103G (AGC→GGC) | cyaA | adenylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (126/106); new base G (9/13); total (135/120) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 260 total aligned reads displayed.
ATTGCGTGTGGATCGCGCGCTTGCTGCTATGGGGCCTGCATTCCAACAGGTCTACAGTCTACTGCCGACATTGTTGCACTATCACCATCCGCTAATGCCGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGG > NC_000913/3991203‑3991736 | tCGCGCGCTTGCTGCTATGGGGCCTGCATTCCAACAGGTCTACAGTCTACTGCCGACATTGTTGCACTATCACCATCCGCTAATGCCGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCct < 2:124042/275‑1 (MQ=255) gcTTGCGTCTATGGGGCCTGCATTCAAACAGGTCTAAAGGTGACTGCCGACATTGTTGCACTATCACCATCCGCTAATGCCGGTTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGAc < 4:562104/274‑1 (MQ=255) aTGGGGCCTGCATTCCAACAGGTCTACAGTCTACTGCCGACATTGTTGCACTATCACCATCCGCTAATGCCGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCGGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTg < 2:230996/275‑1 (MQ=255) ggTTTACGGTCTACTGCCGACATTGTTGCCCTTACACCATCCGCTACTGCGGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCGGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTgg < 4:589936/274‑1 (MQ=255) tACTGCCGACATTGTTGCACTATCACCATCCGCTAATGCCGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGaa > 2:410634/1‑275 (MQ=255) tACTGCCGACATTGTTGCACTATCACCATCCGCTAATGCCGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTCACACGCCTGATGAAACTCAACGCTACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATCTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCTTCTGCCCTGGATATCTGGGTCTGTCATCAACCCTGGCCCGATTGCCaa > 4:185712/1‑275 (MQ=255) aTTGTTGCACTATCACCATCCGCTAATGCCGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAAt > 2:571293/1‑275 (MQ=255) gCACTATCACCATCCGCTAATGCCGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTAc < 2:185712/275‑1 (MQ=255) tATCACCATCCGCTAATGCCGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGCAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACaa > 2:653018/1‑273 (MQ=255) cATCCGCTAATGCCGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGTaa < 4:46272/192‑1 (MQ=255) tCCGCTAATGCCGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCctc > 2:339858/1‑201 (MQ=255) cTAATGCCGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGAc < 4:339858/201‑1 (MQ=255) ggattACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTCCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTg > 4:768488/4‑191 (MQ=255) gtGGAAACTTTTCAAAAGGCATTGTGCTGTACNCGCCGGGCGAAACTAAACGCCACT‑CNTGTAGCGAGCTTGAAATGATTCATGGAATGTCAGGAAAGGATCCGCGGACAGGTGAGCTTCAAATTACTGGTGTAGACACNATGGGCAGCACCTCGGCCGTAGGGGAAAGTTGGTCCTGTGACCTGGATATCTGGGGCTGTCAGAAATCCTTGCTCGATAGCGAAGGGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCcgcg < 3:126647/277‑1 (MQ=255) ggTAACGTTCCCAAAGGCATTTGCCTTTACCCGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGAGCCGGCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCAc < 6:555833/149‑1 (MQ=255) aaCGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTc < 6:66843/149‑1 (MQ=255) aCGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTCAATGTAGCCTGCTGGAAAACTGGGCCGCCTCCCt > 2:157210/1‑274 (MQ=255) ttCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTc > 7:953811/1‑148 (MQ=255) ttCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTc > 7:325711/1‑148 (MQ=255) ccAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTTAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGt < 8:344697/148‑1 (MQ=255) ccAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCGGCCCCTCGTCCGTa < 6:534255/149‑1 (MQ=255) ccAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGt < 8:950546/148‑1 (MQ=255) ccAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGt < 7:282772/148‑1 (MQ=255) ccAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTa > 6:502497/1‑149 (MQ=255) taaaGGCAAATGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAg > 6:233359/2‑149 (MQ=255) cAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTa < 8:242018/148‑1 (MQ=255) aaaGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCGGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTgg > 4:207891/1‑247 (MQ=255) aaaGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAgg > 5:228406/1‑149 (MQ=255) aaaGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCAGGGGCGGCACCTCGTCCGTAg < 7:622407/148‑1 (MQ=255) aaGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAgg > 8:87264/1‑148 (MQ=255) ggCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCa > 6:1001434/1‑149 (MQ=255) gCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCa > 7:1046677/1‑148 (MQ=255) cATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCaaa < 5:644419/149‑1 (MQ=255) cATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCaa > 8:765471/1‑148 (MQ=255) tttGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTGTCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCaa < 7:401856/146‑1 (MQ=255) tttGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCaa > 8:401856/1‑146 (MQ=255) cTTTATACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAATGTGAGCTTCCAATTACTGGTGTATACACCATGGGCGGCACCTCGTCCGTAGGGCAAAGTTGTTc < 5:303958/149‑1 (MQ=255) cTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGCGCTTGAACTGTATCGTGCAATGTCAGTACAGGATCCGCCGAAAGGTGAGGTTCCAAATACTGGTGTATAGACCATGGGCAGCACCTCGACCGTAGGGCAACGTTGTTc < 6:714689/149‑1 (MQ=255) cTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTc > 5:878702/1‑149 (MQ=255) cTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTc < 5:1031921/149‑1 (MQ=255) tttACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTc > 7:712645/1‑148 (MQ=255) ccTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCt < 6:317394/149‑1 (MQ=255) atgaAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGAc > 5:496565/1‑143 (MQ=255) gaAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGatat > 5:774451/1‑149 (MQ=255) aaaCTCAACGCCACTACCTG‑AACGAGCTTGAACTGAATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCGGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGatat > 8:1001278/1‑148 (MQ=255) cTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTg < 8:1046677/148‑1 (MQ=255) aCGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCGGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTc < 7:383265/148‑1 (MQ=255) aCGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCt < 5:590135/149‑1 (MQ=255) ccACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTc < 2:276074/275‑1 (MQ=255) cACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAgttgtt < 7:915837/121‑1 (MQ=255) cACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAgttgtt > 8:915837/1‑121 (MQ=255) cACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGtca > 6:429265/1‑149 (MQ=255) cTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCACGATAGCGAAGAGCGCCAATTGCTa < 1:68336/184‑1 (MQ=255) tACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAAGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTc > 6:131545/1‑101 (MQ=255) cTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATc > 5:623271/1‑149 (MQ=255) aaCGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGCAAGGTTAGCTTCCAATTACTGGTGTATACATCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTATGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGTAGAGCGCCAATTGCTACAACGTAAATGTAACCTGCTGCAACACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCTGGATTGATGAAACCCCCTTCCGTCATAATGAAAg > 4:293247/1‑275 (MQ=255) aaCGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCt < 7:166043/148‑1 (MQ=255) aaCGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTg > 5:425071/1‑149 (MQ=255) gCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATc > 6:604156/1‑141 (MQ=255) cTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGctt > 2:727141/1‑230 (MQ=255) cTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAg > 4:527385/1‑275 (MQ=255) ttGAAGTGGATCGTTGAATGGAAGTGAAGGATCCGCCGAAAGGTGAGCTTCCAATTACGGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGAAAAGTTGTTCCTCTGAACTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTgg < 1:127738/279‑1 (MQ=255) tGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATa < 5:1050830/149‑1 (MQ=255) tGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGaa > 6:493240/1‑149 (MQ=255) tATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCct > 1:33608/1‑100 (MQ=255) aTCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAagag < 6:900953/149‑1 (MQ=255) aTCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAaga > 8:437806/1‑148 (MQ=255) tGGAATGTCAGTACAGGATCCGCCGAAAGGTTAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGATAGCGGCAGCCAGGGGGGCGaa > 2:611161/1‑274 (MQ=255) tGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCCCCTCCCTGGGTGTGGAATTAAGCTTATTCCTGATGGCTTACAACCGCCTCGGAAAAAATNAAAAANNCAGCGGCGGGGGGGaaaggtggtggc > 3:17541/1‑269 (MQ=255) gTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGc > 5:363579/1‑149 (MQ=255) tCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGaa > 5:907199/1‑135 (MQ=255) tACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACa > 7:427766/1‑148 (MQ=255) ctggtacccccgAAAGGTGTGTGTCCAATTCCTTGTGGATACACCATGGGGAGCACCGCGTCCGTAGGGCAAAGTTGTTCCTCTGAGCTGGATATCTGGGTCTGTCATCAATCCTTGCTCGATAGCGAAGAGCGCCAATTGCTACAACGGAAATGTAGCCTGCTGGAAAACTGGGCCACCTCGCTGGGTGTGTAAGTCAGCTTCTTCCGGATTGCTGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTTGGGGGCGAAGATTGTGGCTCCAc < 4:157210/266‑1 (MQ=255) aGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCGGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACg < 8:888101/148‑1 (MQ=255) gtCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTaaa > 6:913075/2‑149 (MQ=255) aTCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTaa < 7:724514/148‑1 (MQ=255) gccgAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTCTGGCTCCACCCAGCata > 2:469548/1‑275 (MQ=255) tGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTgg > 5:164787/1‑149 (MQ=255) gAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTcc < 6:212725/63‑1 (MQ=255) aGCTTCCAATTACTGGTGTATACACCATGGGCGGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGaa > 6:357382/1‑149 (MQ=255) tCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAAt < 7:939804/89‑1 (MQ=255) aaTTACTGGTGTATACACCATGGGCGGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTgg > 7:554496/1‑148 (MQ=255) aaTTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTgg < 8:67636/148‑1 (MQ=255) aTTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGgc < 6:970506/149‑1 (MQ=255) aTTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGgc < 5:954934/149‑1 (MQ=255) ttACTAGGGTATACACCATGGGCAGACTCTGGTCCGTAGGGAAAAGTTGTTCCTCTGACCTGGAAACCTGGGTCTGTCATCAGTCCTGGCTGGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAAttt < 4:25002/275‑1 (MQ=255) atGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAg > 7:237386/2‑127 (MQ=255) gggttttatacCCTGGGCGTCACCCGGTCCGTAGGGCAAAGTGGTTCCCCTGACCTGGGTATATGGGTCTTGCATCCATCCTGGCTCGATAGCGAAAAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTCATGAAAACCGCTTCCGGCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATc < 4:611161/265‑1 (MQ=255) cccATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTGCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGgtg < 8:230746/147‑1 (MQ=255) ccATGGGCGGCACCTCGTCCGAAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTATGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTCCAACGTAGATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTgtgtgt > 8:660090/1‑148 (MQ=255) ccATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTg < 8:562577/143‑1 (MQ=255) cATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATtgt > 2:689461/1‑223 (MQ=255) cATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACGTCTGCTTGCCGAATTTTATCGTACCGCCGttc > 4:253232/1‑273 (MQ=255) aTGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGAGTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCTTATACTTCTGCTTGACGAATTTTATCGTACCGCCGTACg > 2:366056/1‑275 (MQ=255) tGGGCGGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAg > 4:85602/1‑196 (MQ=255) tGGGCATCACGTCTTACGTAGGGCAAAGTTGTTCCGCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGtctc < 1:14121/278‑1 (MQ=255) tGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGaa > 5:525211/1‑149 (MQ=255) gggCAGCACCTCGTCCGTAGGGCAAAGTTGTTNNNCGTACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATGGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCg < 3:217433/278‑1 (MQ=255) gggCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTNGGTGTGGAAGTCAGCTTCTTCCGGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGGNGATGGTGGCGCCACCCAGCAGATACTGCTGNNTGACGAAATTTACCGTAACGCCGTGCGtaccng > 3:96920/1‑274 (MQ=255) gggCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATAGTCCCGCCGTGCGTCTCg > 1:217433/1‑278 (MQ=255) | ATTGCGTGTGGATCGCGCGCTTGCTGCTATGGGGCCTGCATTCCAACAGGTCTACAGTCTACTGCCGACATTGTTGCACTATCACCATCCGCTAATGCCGGGTTACCTTGATGGTAACGTTCCCAAAGGCATTTGCCTTTACACGCCTGATGAAACTCAACGCCACTACCTG‑AACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATCCGCCGAAAGGTGAGCTTCCAATTACTGGTGTATACACCATGGGCAGCACCTCGTCCGTAGGGCAAAGTTGTTCCTCTGACCTGGATATCTGGGTCTGTCATCAATCCTGGCTCGATAGCGAAGAGCGCCAATTGCTACAACGTAAATGTAGCCTGCTGGAAAACTGGGCCGCCTCGCTGGGTGTGGAAGTCAGCTTCTTCCTGATTGATGAAAACCGCTTCCGTCATAATGAAAGCGGCAGCCTGGGGGGCGAAGATTGTGGCTCCACCCAGCATATACTGCTGCTTGACGAATTTTATCGTACCGCCGTGCGTCTCGCCGG > NC_000913/3991203‑3991736 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |