| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,082,696 | G→C | 5.9% | Q22E (CAG→GAG) | fdoH ← | formate dehydrogenase‑O, Fe‑S subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,082,696 | 0 | G | C | 5.9% | 942.3 / 40.4 | 275 | Q22E (CAG→GAG) | fdoH | formate dehydrogenase‑O, Fe‑S subunit |
| Reads supporting (aligned to +/- strand): ref base G (122/135); new base C (12/4); total (136/139) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.96e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-03 | |||||||||||
Only 100 of 276 total aligned reads displayed.
GCACGCTTTCAGGCAGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATACGTTTGGTCACCA > NC_000913/4082424‑4082965 | tacagttgtgaGGCAGCCTGGATCTTGACGGTTNNNAAACCCGTGTTTGCGGATCAGCCATGCCATTTTTTCTTTCTGCTCCACTTCCGAGAAGAGCATTACCGTCCACGATGTGTCGCTTAAAGCATTGGGGTTGTCGTACACCCCAATGTTATGGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgcgc < 3:245635/269‑1 (MQ=37) gCTTTCCGGCAGACTGGATCGGAACAGGGCAGACAGCCGTCTTTGCGGATCACCCCTTACATTTTTTCGTTCTGCTCCCCTTCCGAGAAGCGCATTACCGTACACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGGGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgcgc < 4:220207/275‑1 (MQ=38) cTTTCAGGCAGCCTGGATCGGAACAGTTCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGcgcg < 2:395181/275‑1 (MQ=255) ccTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACCGCCCATACAGGTGGTAACGTCGCTTGGTTTCGCCACCTCTTTCTGGACTTCCCGCGCCTACGGCCCgggcattacag > 4:295578/1‑266 (MQ=255) ccTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAgcc > 4:434069/1‑272 (MQ=255) tggaCAGTGCATCTGGCCGGCTTTGCGGAGCGGCCATTCCAGTTTGGCTTTCGGCTCCACTTCCGAGGAGGGCAGTACCGTCCACGATTTGGCGCTTAAAGCATTGGGGTTTGCGTACCCCGCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTcg < 4:12386/272‑1 (MQ=38) tGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGCGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACCCGCCACCTGACAGGCTTTCCAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTCCTTCCTGGTAGTCCCGCCCCTTACGCCCGGGGCCCAGCCCGTTAGCCGCGGaaag > 4:416444/1‑273 (MQ=255) tGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGGGGAacg > 2:113038/1‑275 (MQ=255) tGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCTGAacg > 2:719082/1‑275 (MQ=255) tGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacg < 2:453352/275‑1 (MQ=255) cAGCCGTCGTGGCGGTTCGGCCCTTCCCGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGGGCTTAAATCATTGGGGTTGTCGTACCCCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAAt < 4:11202/275‑1 (MQ=255) cGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTATTCGCGGAACGACGAataata > 2:667530/1‑275 (MQ=255) tCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACCGCCGATACAGGTGGTAACGTAGGTGAGTTTCGACACTTCTTCCTGGAAGTCCCGCGACTGGGGCGCGGGGGTCAGACCGGTGGACGCGGGACGAGCAATGatata > 4:652362/1‑274 (MQ=255) tCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGGCCCTGAGGCGCGGGGGTCAGACCGGTAGTCGCGGAGGGACGAAGGatagt > 4:691646/1‑273 (MQ=255) aGCCATTCCAGTTTGTCGTTCTGCTCCCCTACCGGGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGAt < 2:427805/274‑1 (MQ=255) aGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGAt < 2:494281/274‑1 (MQ=255) cATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGCGGCGCGGTGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCCTGAGATTGATAAGCCATATGTCGTCTCCTTTACACCTTTTCCACATTCACcaggaa > 1:16797/1‑279 (MQ=255) aCCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga < 2:416444/274‑1 (MQ=255) tCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTa > 1:96385/1‑278 (MQ=255) cATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGa < 6:178221/149‑1 (MQ=255) aTTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAg < 6:367445/149‑1 (MQ=255) aTTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAg < 5:605554/149‑1 (MQ=255) gggTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgcg > 8:15107/1‑148 (MQ=255) ttGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCggg > 6:93211/1‑149 (MQ=255) ttGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCgg < 8:305267/148‑1 (MQ=255) tGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTCAGGCGCggga < 6:644285/149‑2 (MQ=255) gagcgccccAATGGTATTGTCGACGGTATCGCGAATGTCGTGCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACGGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGCCCCGCGCCTGAGGCGCGGGGGTCAGACCGCTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGaa < 1:236213/274‑1 (MQ=255) tACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTCCAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCa > 8:736950/1‑148 (MQ=255) ttATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTcgc > 5:408351/1‑149 (MQ=255) tATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTcgc > 7:11071/1‑148 (MQ=255) ttGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTcgc < 6:836990/146‑1 (MQ=255) ttGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTcgc > 5:836990/1‑146 (MQ=255) gACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacgac > 6:98565/1‑149 (MQ=255) aCGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacgacg > 5:482318/1‑149 (MQ=255) ggTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGaa < 5:542719/149‑1 (MQ=255) cgcgAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGat > 7:631656/1‑148 (MQ=255) cgAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGatat < 8:930569/148‑1 (MQ=255) cgAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGatat > 7:593746/1‑148 (MQ=255) cgAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGatat < 8:843567/148‑1 (MQ=255) aTGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGatat < 8:436230/145‑1 (MQ=255) gTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGCAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCCACGAATGGCGTTAAAGTATTGGCAATAAAGCCttttt > 2:36877/1‑275 (MQ=255) tCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTgag < 7:248724/148‑1 (MQ=255) cGTTCCACCCTGAAAACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGGAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCAGATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATGGGCAATAAAGCCTTTTTTAGCTa < 1:151743/279‑1 (MQ=255) tCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTg > 8:232663/1‑148 (MQ=255) tCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACCTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGAt > 8:381668/1‑146 (MQ=255) tCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACCTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGAt < 7:381668/146‑1 (MQ=255) ccatggAACACCGCACCGGACAGCGTTTACAGCCGATACCGGGGGGAACTGTGATGGGTTTCTCCCCTTCTTCCTGGAAGTCCCGCGCCTGAGGTGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACg < 4:36877/270‑1 (MQ=255) aCTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTcgc > 5:379168/1‑118 (MQ=255) tctGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc > 5:745278/1‑149 (MQ=255) tctGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGc < 2:691646/274‑1 (MQ=255) cGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgt > 7:432762/1‑148 (MQ=255) gCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGAt > 2:582800/1‑132 (MQ=255) cTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGt > 2:281436/1‑275 (MQ=255) cAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGtt > 7:1025372/1‑148 (MQ=255) agggCTTCAAAGCCGGTAACGGTGGTAACGTCGATGGGTTTCGCCCCTTCTTCCTGGAAGTCCCGCGCCTGAGGCTCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAAt < 4:719082/273‑1 (MQ=255) aGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTac > 5:307962/1‑149 (MQ=255) aGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATATCCCCAGGGAAtacga > 1:60246/1‑274 (MQ=255) ggCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTac > 7:799317/1‑148 (MQ=255) gCTTTACAGCCGATACCGGGGGTAACGTCGAGGGGTTTCGCCCCTTCTTCCTGGAAGTCCCGGGCCTGAGGGGGGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCgg < 4:602237/275‑1 (MQ=255) cTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGa < 2:184401/275‑1 (MQ=255) cAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCtttt > 8:463997/1‑148 (MQ=255) gCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTga > 5:879771/1‑109 (MQ=255) cGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACGCCtttacaac < 8:564092/148‑6 (MQ=255) aTACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCAAATTCACAAGGAAGGACTTAAACTCCGGCGTCTGAGGGTTCGCATCACCAACNAATGGCGTTAAAGTATTGGACATAACGCCTTTTTTAGCTACGCCTTCCAACCCCCAATTAATCAGAATACCAAttggtta > 3:190934/1‑272 (MQ=255) aCAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCTTCCCCAACGAATGGCGTGAAAGAATGGGCAATAAAGCTTTTTTTACCTACGCACTCCTAGCCCCAGTGACACGCACAACCGATGTTag > 4:152462/1‑274 (MQ=255) aGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTcaca > 6:232259/1‑149 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATACAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGAAGGTATcta > 2:623518/1‑271 (MQ=255) gtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaa > 6:867802/1‑149 (MQ=255) gtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagga < 7:490442/148‑1 (MQ=255) tAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaag < 6:278056/149‑1 (MQ=255) tAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaa < 8:670231/148‑1 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaagg < 8:593746/148‑1 (MQ=255) cGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAaggaagga < 7:463997/148‑1 (MQ=255) gTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGaa > 6:42993/1‑80 (MQ=255) cGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTTAATCGGAATACCGATGGTATCGATATCTTTGCCg > 2:86138/1‑275 (MQ=255) gTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCcac < 6:466282/124‑1 (MQ=255) tttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCcac > 8:640842/1‑123 (MQ=255) tttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCCCAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTACCTAAACTTTTCCCTTTCGCt > 4:537765/1‑274 (MQ=255) tttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCg > 6:163413/1‑149 (MQ=255) aCTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAg > 7:683009/1‑85 (MQ=255) aCTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAg < 8:683009/85‑1 (MQ=255) cttcttCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTg > 7:897453/1‑148 (MQ=255) tcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCgt < 5:433473/149‑1 (MQ=255) ttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGAACGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCgtgt > 6:381255/1‑149 (MQ=255) tcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGaa < 4:95858/274‑1 (MQ=255) ccTGGAAGTCCCGCGCCTGAGGCGCGGGGGCCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACCAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCCCCAACGAATGGCGTTAACGTATTGGCATTAAAGCCTTTTTTAGCTACGCCTTCATATCCCCAGTGAATCGGAATACCGATGGTACCGATATCTTTGCCGTTCGCTTCCAGCGTGGGAAt > 4:251675/1‑274 (MQ=255) cTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacgac > 5:703334/1‑53 (MQ=255) cTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAATGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCg > 6:316488/1‑149 (MQ=255) cTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCg > 6:476954/1‑149 (MQ=255) gTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAAcgacga > 4:588634/1‑49 (MQ=255) cccccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCa > 8:895956/3‑148 (MQ=255) ccccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCAcc > 5:950695/2‑149 (MQ=255) ccccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCAcc > 6:581628/2‑149 (MQ=255) cccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaag < 5:10839/113‑1 (MQ=255) cccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCAcc > 8:579225/1‑148 (MQ=255) ccGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCaa < 5:59638/149‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAAc < 5:249785/149‑1 (MQ=255) gcCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGa < 8:493930/148‑1 (MQ=255) ccTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGaa < 7:736950/148‑1 (MQ=255) ccTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAAt < 6:1020380/149‑1 (MQ=255) | GCACGCTTTCAGGCAGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATACGTTTGGTCACCA > NC_000913/4082424‑4082965 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |