| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,356,446 | A→T | 5.3% | intergenic (‑35/+24) | dtpC ← / ← cadA | dipeptide and tripeptide permease/lysine decarboxylase, acid‑inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,356,446 | 0 | A | T | 5.3% | 611.5 / 18.5 | 174 | intergenic (‑35/+24) | dtpC/cadA | dipeptide and tripeptide permease/lysine decarboxylase, acid‑inducible |
| Reads supporting (aligned to +/- strand): ref base A (76/87); new base T (5/4); total (81/93) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.15e-04 | |||||||||||
Only 100 of 177 total aligned reads displayed.
TCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGTTATCATCAAAACCAAGCTGATGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGGTGATCATTTCACCCGGCATTACCAGAGGAACTCCCGGCGGGTACGGAAGGATCATATTGGCGTTAATACGACCTACCATTTCGTCGAGGTAAACTTCT > NC_000913/4356177‑4356719 | cgCAGTGCGGTTGCCGGGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATTGCATGGTTATCATCAAAACCAAGCTGATGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCAc < 2:424226/270‑1 (MQ=255) ggCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGTTATCATCAAAACCAAGCTGATGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTccc < 2:381037/256‑1 (MQ=255) cagggaATAGGGGTAACGTAAACCAGAGAATCATAGGCGCTGAACAGGCTGATGGCATGGTTATCATCAGAACCAAGCTGATGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCt < 1:89584/274‑1 (MQ=255) gaAGCATATGCGCTGAACAGGCTGATGGCATGGTTATCATCAAAACCAAGCTGATGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGcc > 4:667561/1‑274 (MQ=255) tGGCATGGTTATCATCAAAACCAAGCTGATGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCCCCGTGAATATCGGt > 2:487074/1‑275 (MQ=255) tGGTTATCATCAAAACCAAGCTGATGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCaa > 2:160026/1‑275 (MQ=255) tGGTTATCACCAAAACCAAGCGTATGGGTGGGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCaa < 4:101507/275‑1 (MQ=255) tgTTATCATCAAAACCAAGCTGATGGGTGAGATAGATAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAg < 1:100266/275‑1 (MQ=255) aaCCAAGCTGATGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGt > 2:572878/1‑274 (MQ=255) aTGGGTGGGATAGAGAANGAGTAANGCACGCATGCCGTAAAAACTGAAGTACTCCCATATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAAGGGGAAGTGGCAAGCCACTTcc < 3:36231/156‑1 (MQ=255) aTGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAAGGGGAAGTGGCAAGCCACTTcc > 1:36231/1‑156 (MQ=255) aTGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAg > 4:339077/1‑219 (MQ=255) gTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACtt > 6:955190/1‑149 (MQ=255) gTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTg < 2:339077/219‑1 (MQ=255) gagaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTc < 7:1009278/148‑1 (MQ=255) gagaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTcacac < 4:160026/275‑1 (MQ=255) agaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTccc > 6:211634/1‑149 (MQ=255) gaTAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCt < 5:955190/149‑1 (MQ=255) agaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCTTGCCACTTCCCtttttt < 6:39573/149‑5 (MQ=255) aaTGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACg < 8:626393/148‑1 (MQ=255) aaTGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACg < 7:830955/148‑1 (MQ=255) aTGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCt > 7:774842/1‑141 (MQ=255) aTGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCt < 8:774842/141‑1 (MQ=255) tGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCa < 2:32356/274‑1 (MQ=255) aGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTNTATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGc < 3:40879/191‑1 (MQ=255) aGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTca > 4:686322/1‑259 (MQ=255) aGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTca < 2:686322/259‑1 (MQ=255) aGTAAGGCACGCATGCCGGAAAAACTGAAGTAACCACAGATTGGGATGGCCACGAGATAGTATATCGAGGGCGGCTGTGAGGGGGTTTTCATGGGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAgg < 4:129275/274‑1 (MQ=255) aGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCGAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAatt < 8:453385/148‑1 (MQ=255) cACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTAttt > 5:862579/1‑149 (MQ=255) gCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTg > 4:155627/1‑260 (MQ=255) aTGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGACCCCCGATTTCACACAGCATCTGCCGGAACCCCAGAACCg > 4:551200/1‑275 (MQ=255) tGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCt > 6:798356/1‑149 (MQ=255) ccGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGa < 2:180145/274‑1 (MQ=255) cGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTttc < 5:742796/149‑1 (MQ=255) gTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTttc < 7:450131/148‑1 (MQ=255) tAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTttctt > 6:727061/1‑149 (MQ=255) aaaaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTttcttc < 8:356242/148‑1 (MQ=255) aaaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTcttctt < 5:727061/149‑1 (MQ=255) aaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCttt < 5:6991/149‑1 (MQ=255) gTACTCCCAGATTTGGTTGGCCCGGGTTTAGATTATCGCGGGCGGCTGTGAGGGTGTTTTCATGTGTTTTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGGTg < 4:305094/275‑1 (MQ=255) tACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATAcc < 7:643269/148‑1 (MQ=255) tACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCt > 6:468942/1‑149 (MQ=255) aCTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCtt > 5:49944/1‑149 (MQ=255) cAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTaagc > 8:221092/1‑146 (MQ=255) aGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGt > 5:764345/1‑149 (MQ=255) aTTTGGATCGCCACGATATATGATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGGGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGGTGATCATTTCAc < 2:119651/275‑1 (MQ=255) aTTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGGTGATCATTTCAc < 2:112213/275‑1 (MQ=255) tttGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGtat < 7:803263/148‑1 (MQ=255) tCGCCACGATATAGTGTATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGc > 7:123980/1‑148 (MQ=255) cACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCa < 5:874113/149‑1 (MQ=255) cgtgtttgtagttCGCGGGTGGGTGTGAGGGTGTTTTAATGGTTTTTCCTTTATG‑GCAAAAAA‑GGGAAGTGGCAGGCCCATGCCCTTGTACGGGTTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATGGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGGTGATCATTTCACCCGGCATTACCa < 4:352351/262‑1 (MQ=255) gATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAgg < 2:520122/221‑1 (MQ=255) taGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGAc < 5:275088/149‑1 (MQ=255) aGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTTC‑TTATGAGCAACAAA‑GGGCAGTGGCCAACCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACTTTAACGGTATAGCGGTCAATAGCCTGAc < 8:1017411/148‑1 (MQ=255) tataTCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACgg > 8:55890/1‑148 (MQ=255) tataTCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGt < 6:340847/149‑1 (MQ=255) cgcgcgcgGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCTCTTGTACGAGCTACTTATTTTTTGCTTTCTTCTTGCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACAAAGCATCTGCAGGAACTACAGAACCGGACGGCTTTCTTCGGTGATCATTTACCCCGGCTTTACAAGAGGACCTCCcgg > 4:263569/1‑274 (MQ=255) cgcgcgcgGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCa > 2:773174/1‑108 (MQ=255) cgcgcgcgGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCCCCGTGCATATCGGTGTCAAAGCACGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAAATCCAGAACCGACCGGCTTTCTTCGGTGATCATTTCACCCGGCCTTACCAGAGGAACGCCcggc > 2:727201/1‑275 (MQ=255) cgcgcgcgGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGCATATCGGtt > 2:622636/1‑164 (MQ=255) cgcgcgcgGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGAtt > 1:43669/1‑191 (MQ=255) cgcgcgcgGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGGTGATCATTTCACCCGGCATTACCAGAGGAACTCCcgg > 2:628336/1‑274 (MQ=255) cgcgcgGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGATGATCATTTCACCCGGCATTACCAGAGGAACTCCcggcgg > 2:510915/1‑275 (MQ=255) cgcgGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATAc < 4:773174/108‑1 (MQ=255) cgcgGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCaa < 4:622636/164‑1 (MQ=255) cgGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGa < 4:536020/218‑1 (MQ=255) cgGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCttt < 4:443056/225‑1 (MQ=255) gtgAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTttc > 7:47995/1‑89 (MQ=255) gtgAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTttc < 8:47995/89‑1 (MQ=255) tgAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATc < 7:55890/148‑1 (MQ=255) gAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCGTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCg < 8:678013/148‑1 (MQ=255) aGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGt < 5:468942/149‑1 (MQ=255) gggTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGCAGTGGCAAGTCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGt < 8:82986/148‑1 (MQ=255) gtTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCTTGCCACTTc < 5:996646/51‑1 (MQ=25) ttttCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCaa < 7:1013900/148‑1 (MQ=255) tttCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCaaa > 8:44312/1‑148 (MQ=255) ttCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGGTGATCATTTCACCCGGCATTACCAGAGGAACTCCCGGCGGGTACGGAAGTATCATATTGGCGTTAATa > 4:562250/1‑275 (MQ=255) ttCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGGTGATCATTTCACCCGGCATTACCAGAGGAACTCCCGGCGGGTACGGAAGGATCATATTGGCGTTaa > 4:219444/1‑273 (MQ=255) ttCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACAGGACGGCTTTCTTCGGTGATCATTTCACCCGGCATTACCAGAGGAACTCCCGGCGGGTACGGAAGGATCATATTGTCGTTAATACCAc > 1:215105/1‑279 (MQ=255) tctcC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTAt < 5:346551/115‑1 (MQ=255) tctcC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAg < 7:1029161/148‑1 (MQ=255) ctcC‑TTATGAGCAAAAAA‑GGGAATTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGGTGATCATTTCACCCGGCATTACCAGAGGAACTCCCGGCGGGTACGGAAGGATCATATTGGCGTTAATACg < 2:20021/275‑1 (MQ=255) cc‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGa > 7:321176/1‑148 (MQ=255) tATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAgcg < 8:123980/148‑1 (MQ=255) tGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGGTGATCATTTCAc < 4:19859/210‑1 (MQ=255) gCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGAt < 8:1023155/148‑1 (MQ=255) aaaa‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCaa < 8:868620/122‑1 (MQ=255) aaaa‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCaa > 7:868620/1‑122 (MQ=255) aaa‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTcaca < 6:692836/149‑1 (MQ=255) a‑GGGAAGTGGCTTGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTcacaca > 5:915226/1‑149 (MQ=255) a‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTcacaca < 6:49944/149‑1 (MQ=255) gggAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAAc < 6:637947/68‑1 (MQ=255) gggAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAAc > 5:637947/1‑68 (MQ=255) gAAGTGGCTTGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCa < 6:988907/79‑1 (MQ=39) gAAGTGGCTTGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCa > 5:988907/1‑79 (MQ=39) gAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCa > 5:154385/1‑149 (MQ=255) gTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGGTGATCATTTCACCCGGCATTACCAGAGGAACTCCCGGCGGGTACGGAAGGATCATATTGGCGTTAATACGACCTACCATTTCGTCGAGGTAAActtc < 1:132384/279‑1 (MQ=255) cAAGCCACTTCCCTTGTACGAGCTAATTAGTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGGTGATCATTTCACCCGGCATTACCAGAGGAACTCCCGGCGGGTACGGAAGGATCATATTGGCGTTAATACGACCTACCATTTCGTCGAGGTAAActtc < 2:551200/275‑1 (MQ=255) aGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGGTGATCATTTCACCCGGCATTACCAGAGGAACTCCCGGCGGGTACGGAAGGATCATATTGGCGTTAATACGACCTACCATTTCGTCGAGGTAAActtct > 2:22332/1‑274 (MQ=255) | TCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGTTATCATCAAAACCAAGCTGATGGGTGAGATAGAGAATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCC‑TTATGAGCAAAAAA‑GGGAAGTGGCAAGCCACTTCCCTTGTACGAGCTAATTATTTTTTGCTTTCTTCTTTCAATACCTTAACGGTATAGCGGCCATCAGCCTGACGGTATGCACCGTGAATATCGGTTTCAAAGCCCGGATAGTGAGCGCCGATTTCACACAGCATCTGCAGGAACTCCAGAACCGGACGGCTTTCTTCGGTGATCATTTCACCCGGCATTACCAGAGGAACTCCCGGCGGGTACGGAAGGATCATATTGGCGTTAATACGACCTACCATTTCGTCGAGGTAAACTTCT > NC_000913/4356177‑4356719 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |