| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 53,379 | T→C | 10.3% | E13G (GAG→GGG) | pdxA ← | 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 53,379 | 0 | T | C | 10.3% | 595.2 / 40.7 | 206 | E13G (GAG→GGG) | pdxA | 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (91/91); new base C (14/9); total (105/101) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01 | |||||||||||
Only 100 of 207 total aligned reads displayed.
AGCGTTTCCACCACATAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGT‑GAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAA > NC_000913/53115‑53646 | aTTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGC‑GATCACAACACGTTGGGTTTTaa > 2:737784/1‑275 (MQ=255) cccGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGGTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGAtt < 2:126896/275‑1 (MQ=255) gcggTAGGGGGAAAGGAGGGAGGGTGAATGGGCCAGCAGTTTGCGGTGGTGAAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTgg < 4:47720/272‑1 (MQ=255) cgcgACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACgt > 2:168314/1‑273 (MQ=255) gAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACCACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCCCGCCGGGAGG‑GATCCCAACCCGTTGGGGTTTAAACCATAGTTGCCCAGGATTTTAACTTAGGGCCTGGCCCGTTGTTCCt > 4:11486/1‑272 (MQ=255) gAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATcac > 1:80992/1‑208 (MQ=255) tgtgCCTGCAGGTTGGGGTTGGGAAGGGGAGTTGGGGGAATAAGGGCGGAGGTGGAGCGGCAAACCGAGCATCGCGGCCCGGTTGGTAAGGAGGGGGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGACCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTtgctg < 4:291254/274‑1 (MQ=255) gtgCCCGCAGGTTGGAGGTGTGAAGGGGAGGTGGGGGAGTAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCTCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTtgctgc < 4:2671/274‑1 (MQ=255) cccGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCNCGCTGTGCAAGCTGGACAACTAAGTCCGGGCAACTCCCGGCGGGCTCGCCGGGAGG‑GACCACAACACGTNGGGTTTTAACAATTAGGTGCCCAGGAGTTTAACACAGCCCCTGGCNCNTAGTTTCCTCATCCCGCTTGCCGCCtcctccc > 3:56907/1‑278 (MQ=255) tgCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACCCACGCTGTGCAAGCTGGACAACTAAGTCNGGGCCAATCCCGGCGGGCCCGCCCGGAGT‑GATCACAACACGTTGGGTTTTTACCATTAGTTGCGCAGGATTTTANAGTAGGCGCTGGAATGTTTGTGCTGCATCCNNCTCTCTCCTCCTTCCGATAACTTACGTTtcanc > 3:105446/1‑279 (MQ=255) aGGGGAGTGGGGGGAAGAAGGGGGGGGGGTGAGCGGCAAACCGAGAATCGCTGCCCGGTTGGGAAGGCGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTtca < 4:608685/274‑1 (MQ=255) ggggAGGTGGGGGAATAAGGGGGGGGGGTGAGCGGCAAACCGGGCATCGCTGCCCGGTTGGGAAGGAGGGTGGCATCGGCACAAACAACCAGTTCGACCGGCACCTCACGCGTGGCAAGCTGGACAACGAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTGCGTCCGAGAACTTACGGTtcatc < 4:159216/275‑1 (MQ=255) gAGTTGGGGGAATAAGGGCGGATGGTGGGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATg < 1:105446/278‑1 (MQ=255) ttGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTc < 6:310441/149‑1 (MQ=255) gggggAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGc < 2:47149/274‑1 (MQ=255) aaTAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCggg < 8:505803/148‑1 (MQ=255) aaTAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCggg > 8:567131/1‑148 (MQ=255) aTAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGAGCCCGCCGGGa < 7:125880/148‑1 (MQ=255) ctcGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGACACTCACGCTGTGCAAGCTGGACAACTCAGTCCGGGCCACTCCCGGCGGGCTCGCCGGGAGT‑GAt < 7:657740/146‑1 (MQ=255) gCGNNGGGTGAGCGGCAAACCGGGCATCGCTGCCCGGTTGGTAAGGAGAGTGGNATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACg < 4:737942/206‑1 (MQ=255) gcgggTGAGCGGCAAACCGAACATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCAGCACAAACAACCAGTTCGACCGGACACTCTCGCTGTGCAAGCTGGACAAGTAAGTCAGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATcaag > 8:657740/3‑146 (MQ=255) gAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGCGT‑GATCACAACCCGCTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACTGTTCATCAGCATGCGGTATGCCCGATCttt > 2:240091/1‑275 (MQ=255) gAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCttt > 2:443910/1‑275 (MQ=255) gAGGGTGAGCGGCAAACCGAGCATCGCTGACCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGCCCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATcaca < 7:788302/148‑1 (MQ=255) ggTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTa < 1:140894/240‑1 (MQ=255) gTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAacac > 7:223812/1‑148 (MQ=255) gCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTg < 7:423335/148‑1 (MQ=255) cttaccaaCCGGGCAGCGATGCTCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTgg > 5:769574/7‑149 (MQ=255) taccaaCCGGGCAGCGATGCTCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTggg > 8:276357/5‑148 (MQ=255) gCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGtt < 5:531724/149‑1 (MQ=255) gCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGAGCCCGCCGGGa < 6:438451/128‑1 (MQ=255) aCCGGGCAGCGATGCTCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTaa > 6:669248/1‑149 (MQ=255) aCCGGGCAGCGATGCTCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTaa > 5:190478/1‑149 (MQ=255) aCCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTaa > 6:991371/1‑149 (MQ=255) aCCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTa < 7:840949/148‑1 (MQ=255) aTCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAg > 6:600867/1‑149 (MQ=255) aTCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATGAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATc < 2:490740/274‑1 (MQ=255) cccGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTc < 7:1055187/148‑1 (MQ=255) cccGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCa < 6:713024/149‑1 (MQ=255) ccGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCa < 7:710666/148‑1 (MQ=255) ccGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAg > 6:354640/1‑149 (MQ=255) tGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGAtt < 8:29995/148‑1 (MQ=255) aaGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAAc < 6:190478/149‑1 (MQ=255) gagTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGCTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCCTGCGGTATGCACGATCTTTCTGCGCAGCGTCGGttt > 4:390611/1‑248 (MQ=255) ggCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGCTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATc < 2:390611/248‑1 (MQ=255) tCGGCACAAACTACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCa > 5:797209/1‑149 (MQ=255) tCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCCCGCCGGGAGT‑GATCACAACACGTTGGGCTTTAACCATTAGTTGCTCAGGATTTTAACGCAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGATAACCTACGGTTCACTAGCATCCGGTCTGCCCCATGTTTCTGCGCAGCGTCGGTTTTATCACCATTACGGGTCTCCAGCAGTTCTATTcaa > 4:52161/1‑275 (MQ=255) cGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCa > 8:420308/1‑148 (MQ=255) acaaacaaCCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTg < 8:856187/141‑1 (MQ=255) acaaacaaCCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACgttg > 6:938544/1‑149 (MQ=255) caaacaaCCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACgttg > 8:776022/1‑148 (MQ=255) aacaacCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTc < 5:171196/149‑1 (MQ=255) aacaacCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTc > 5:260572/1‑149 (MQ=255) aacaacCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGCACTTCCGGTTCATCAGCATGCGGTATGCAGGATCTTTCTGCTCAGCGTCGGGTTTATCGACATTACGGGTAGTCAGCCGTTCGATTAAATGACacgc > 4:463765/1‑272 (MQ=255) caacCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCt < 5:991371/149‑1 (MQ=255) acCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTg > 8:164900/1‑148 (MQ=255) ccAGTTCGACCGGCCACTCAGGCTTTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATg < 2:660646/275‑1 (MQ=255) cAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTa < 7:183814/125‑1 (MQ=255) aGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCt > 8:720735/1‑130 (MQ=255) tCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCa > 8:227233/1‑148 (MQ=255) tCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAg < 5:711963/149‑1 (MQ=255) ggCCACTCACGCTGTGCAAGCTGGCCAACTAAGTCCGGGTCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCCTCCAGCTtgc < 7:697354/148‑1 (MQ=255) gCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCgg > 4:697911/1‑60 (MQ=255) gCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGT‑GAAcc > 2:268197/1‑274 (MQ=255) cTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGt < 2:697911/60‑1 (MQ=255) gggCAAGCGGGACAACTAAGACCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTAACGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGT‑GAACCAGTGCACTCATTTGAc < 1:87581/276‑1 (MQ=255) tgCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCgaga < 7:567131/148‑1 (MQ=255) cAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATg < 1:196472/173‑1 (MQ=255) cAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATg > 3:196472/1‑173 (MQ=255) ctgtGGACAACTAAGTCCGGGGCAATCCCGGCGGGCGTGCCGGGAGTGGTTCAAAAC‑CGTTGGGTTTTAACCATTTGTTGCTCAGGAATTTAACGTTGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGGTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGT‑GAACCGGTGCACTCATTTGACCt < 4:688361/272‑1 (MQ=255) gACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGtt > 5:627715/1‑149 (MQ=255) aCAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGtt > 7:910191/1‑148 (MQ=255) aCTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTtcat < 7:276357/148‑1 (MQ=255) cTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTtcatca > 6:106280/1‑149 (MQ=255) tAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCGCAGGATTTTAACGTAGGCGCTGGCACGTTTTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGCATGCCCGATCTTTCTGCCCAGCGTCCGTTTTATCCACATTACGGGTATCCAGCAGGTCGATTAAATTCCAGCCTACTGAA‑AGTGGAAANAGTGTNNTCATTTTACCTTTGttttgtcgaggc > 3:14699/1‑269 (MQ=255) aaGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGc < 6:504718/149‑1 (MQ=255) aaGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTCTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAg > 8:703597/1‑148 (MQ=255) tCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGT‑GAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTc > 4:437542/1‑275 (MQ=255) cGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCg < 5:332059/149‑1 (MQ=255) ggCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTtcat < 8:587302/138‑1 (MQ=255) ggCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTtcat > 7:587302/1‑138 (MQ=255) gCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAACCGAATGAAGAGT‑GAACCGGTGGAACCATTTGACCTTTTTTCAGGCCAggcttgt > 4:80303/1‑268 (MQ=255) gCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACAGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACCTTACGGGTATCCAGCAGTTCGATTAAATGCCCGCCGAATGAAGAGT‑GAACCTGTGCACTCATTTGACCTTTGTTCAGGGGAGTTAGGTCGTCa > 1:74353/1‑279 (MQ=255) cAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTAt > 7:384710/1‑148 (MQ=255) cAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCAGCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGT‑GAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGt < 2:330745/275‑1 (MQ=255) actCCCGGCGAGCCCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACCTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGt < 5:922602/128‑1 (MQ=255) actCCCGGCGAGCCCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTGGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGt > 6:922602/3‑130 (MQ=255) aaTCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATg < 7:988878/148‑1 (MQ=255) ctCCCGGCGAGCCCGCCGGGATT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCtt > 7:521440/2‑104 (MQ=255) aTCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGT‑GAACCGGTGCACTCATTTGACCTTTGt < 2:80303/255‑1 (MQ=255) tCCCGGCGAGCCCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCAc > 6:102079/1‑149 (MQ=255) cccGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCAc > 8:142243/1‑148 (MQ=255) cccGGCGAGCCCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCa > 7:148722/1‑147 (MQ=255) cccGGCGAGCCCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCAc > 8:222774/1‑148 (MQ=255) cggcggGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGAt < 5:1000790/149‑1 (MQ=255) ggcggGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTtg < 8:117203/100‑1 (MQ=255) gcggGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCt < 5:357371/149‑1 (MQ=255) gcggGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCt < 6:639766/149‑1 (MQ=255) gggCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCttt < 6:260572/149‑1 (MQ=255) gCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCt > 5:978025/1‑149 (MQ=255) | AGCGTTTCCACCACATAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT‑GATCACAACACGTTGGGTTTTAACCATTAGTTGCTCAGGATTTTAACGTAGGCGCTGGCACGTTGTTCCTGCATCCAGCTTGCTGCTTCTTCCGAGAACTTACGGTTCATCAGCATGCGGTATGCACGATCTTTCTGCGCAGCGTCGGTTTTATCGACATTACGGGTATCCAGCAGTTCGATTAAATGCCAGCCGAATGAAGAGT‑GAACCGGTGCACTCATTTGACCTTTGTTCAGGCGAGTCAGGGCGTCACGGAA > NC_000913/53115‑53646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |