BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,560,330	G→T	5.3%	C25F (TGC→TTC)	yffL →	CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,560,330	0	G	T	5.3%	543.2 / 14.9	151	C25F (TGC→TTC)	yffL	CPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (81/62); new base T (4/4); total (85/66)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.03e-01

Only 100 of 152 total aligned reads displayed.

TTTGTTTTGTGTGGTTTAACTATCACCTGATAAGAAACCTGTATTCAATTTGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACC‑TGCGCTACCTGGACACATTAAACCGACTT  >  NC_000913/2560117‑2560589

tttgttttgtGTGTTTTAACTATCACCTGATAAGAAACCTGTATTCAATTTGCCGGATTCGTCCGGCTGTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTGTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCACGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAAc                                                                                                                                                                                                     <  1:144193/279‑1 (MQ=255)

tttgttttgtGTGGTTTAACTATCACCTGATAAGAAACCTGTATTCAATTTGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTa                                                                                                                                                                                                         >  4:156735/1‑275 (MQ=255)

        gtgtgGTTTAACTATCACCTGATAAGAAACCTGTATTCAATTTGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCGGCAACATGCCGGGGGCGGTATGAGAAACAAGCAAAAGGAGAATTTCCCAGGTCGGATATGCTAGCAGTAGAAAAAGa                                                                                                                                                                                                 >  2:646650/1‑275 (MQ=255)

                            gATAAGAAACCTGTATTCAATTTGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGGAGAACAAGAAACAAACAATGATGTTCGTc                                                                                                                                                                             >  2:452411/1‑275 (MQ=255)

                                                                      tcttttTCGCTCATTATTCCATTATTCCAATTAAGTAGAATAAATTATTAAATATCAAGAACTTAAATCCATGTTTGCCACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCgg                                                                                                                                                                                                                                                                  <  7:342155/148‑1 (MQ=255)

                                                                      tcttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggg                                                                                                                                                                                                                                                                 <  5:724313/149‑1 (MQ=255)

                                                                                tAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATga                                                                                                                                                                                                                                                       >  5:240008/1‑149 (MQ=255)

                                                                                      ttccattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGAGGTATGAGAAACa                                                                                                                                                                                                                                                 >  6:758908/1‑149 (MQ=255)

                                                                                         cattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGc                                                                                                                                                                                                                                              <  6:389719/149‑1 (MQ=255)

                                                                                             attccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTaaa                                                                                                                                                                                                                                          <  5:248603/149‑1 (MQ=255)

                                                                                             attccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTaa                                                                                                                                                                                                                                           <  8:212466/148‑1 (MQ=255)

                                                                                              ttccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTaaa                                                                                                                                                                                                                                          <  8:3548/148‑1 (MQ=255)

                                                                                              ttccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAAAGACAAAACACgaaa                                                                                                           >  4:399291/1‑274 (MQ=255)

                                                                                               tccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCGGGGGctttta                                                                                                                                                                                                                                                         <  8:731783/132‑6 (MQ=38)

                                                                                                  aaTTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGc                                                                                                                                                                                                                                                              <  8:651756/124‑1 (MQ=255)

                                                                                                   aTTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGgaga                                                                                                                                                                                                                                     <  7:209187/148‑1 (MQ=255)

                                                                                                       aGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAAttt                                                                                                                                                                                                                                 <  7:218776/148‑1 (MQ=255)

                                                                                                         tGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCt                                                                                                                                                                                                                                             <  6:924100/134‑1 (MQ=255)

                                                                                                         tGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtctc                                                                                                                                                                                                                              >  6:820282/1‑149 (MQ=255)

                                                                                                         tGGAATAAAATATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGACACAAGCt                                                                                                                                                                                                                                             >  5:924100/1‑134 (MQ=255)

                                                                                                          ggAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCa                                                                                                                                                                                                                             <  6:997103/149‑1 (MQ=255)

                                                                                                            aaTAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCAt                                                                                                                                                                                                                            >  7:712682/1‑148 (MQ=255)

                                                                                                            aaTAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATg                                                                                                                                                                                                                           >  6:106038/1‑149 (MQ=255)

                                                                                                             aTAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATa                                                                                            >  4:557708/1‑275 (MQ=255)

                                                                                                                aaTTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTg                                                                                                                                                                                                                       >  6:600095/1‑149 (MQ=255)

                                                                                                                 attattCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGa                                                                                                                                                                                                                      <  6:53138/149‑1 (MQ=255)

                                                                                                                     ttCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAAGTTCTCAt                                                                                                                                                                                                                            <  1:40306/139‑1 (MQ=255)

                                                                                                                      tCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCGCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATg                                                                                                                                                                                                                  <  7:417719/148‑1 (MQ=255)

                                                                                                                      tCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGaa                                                                                                                                                                                                                                    >  3:8409/1‑130 (MQ=255)

                                                                                                                      tCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGaa                                                                                                                                                                                                                                    <  1:8409/130‑1 (MQ=255)

                                                                                                                      tCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATg                                                                                                                                                                                                                  <  8:312464/148‑1 (MQ=255)

                                                                                                                      tCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGc                                                                                                                                                                                                                 <  6:125596/149‑1 (MQ=255)

                                                                                                                         atatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTa                                                                                                                                                                                                               >  7:783472/1‑148 (MQ=255)

                                                                                                                             cAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAg                                                                                                                                                                                                           <  7:968596/148‑1 (MQ=255)

                                                                                                                             cAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGt                                                                                                                                                                                                          >  6:216735/1‑149 (MQ=255)

                                                                                                                              aaGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGt                                                                                                                                                                                                          <  8:542732/148‑1 (MQ=255)

                                                                                                                                    ttAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACaa                                                                                                                                                                                                   >  6:37986/1‑149 (MQ=255)

                                                                                                                                      aaaTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAg                                                                                                                                                                                                  <  7:86175/148‑1 (MQ=255)

                                                                                                                                      aaaTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACCCCAGATAATCAAGAGGAAACAGATCATCAcaa                                                                   >  4:331689/1‑273 (MQ=255)

                                                                                                                                         tCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACaa                                                                                                                                                                                                                                                <  8:409696/99‑1 (MQ=255)

                                                                                                                                         tCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACaa                                                                                                                                                                                                                                                >  7:409696/1‑99 (MQ=255)

                                                                                                                                         tCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaac                                                                                                                                                                                              >  5:300800/1‑149 (MQ=255)

                                                                                                                                          ccATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaac                                                                                                                                                                                              >  7:366303/1‑148 (MQ=255)

                                                                                                                                            aTGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacaaa                                                                                                                                                                                           <  5:600095/149‑1 (MQ=255)

                                                                                                                                              gTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTACAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAACTTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTACACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCaaaa                                                            >  2:608508/1‑274 (MQ=255)

                                                                                                                                              gTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACa                                                                                                                                                                 >  1:105261/1‑173 (MQ=255)

                                                                                                                                              gTTTGCAACGAAAGAGCCAGAGTTCGAAAATAGGNACAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACa                                                                                                                                                                 <  3:105261/173‑1 (MQ=255)

                                                                                                                                                     aCGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatgatg                                                                                                                                                                                   >  7:578459/1‑148 (MQ=255)

                                                                                                                                                      cGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGt                                                                                                                                                                                  >  8:127030/1‑148 (MQ=255)

                                                                                                                                                      cGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGt                                                                                                                                                                                  >  7:298633/1‑148 (MQ=255)

                                                                                                                                                       gAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGtt                                                                                                                                                                                 >  8:819044/1‑148 (MQ=255)

                                                                                                                                                       gAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGAGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTc                                                                                                                                                                                <  5:758908/149‑1 (MQ=255)

                                                                                                                                                        aaaGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTc                                                                                                                                                                                >  7:557227/1‑148 (MQ=255)

                                                                                                                                                        aaaGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTc                                                                                                                                                                                >  8:90523/1‑148 (MQ=255)

                                                                                                                                                             tCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTa                                                                                                                                                                                                               >  6:863593/1‑112 (MQ=255)

                                                                                                                                                              ccAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAAt                                                                                                                                                                          >  8:794900/1‑148 (MQ=255)

                                                                                                                                                                agagTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAAtttt                                                                                                                                                                       >  5:191629/1‑149 (MQ=255)

                                                                                                                                                                 gagTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAAtttt                                                                                                                                                                       >  8:743777/1‑148 (MQ=255)

                                                                                                                                                                    ttCGAAAATATGATCAACACAAATCAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGTAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                                                                                                                                                   <  1:32989/149‑1 (MQ=255)

                                                                                                                                                                    ttCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATTTCTGATATGCTATCAGTAGAACAAGANACAAACANTGANGTTCGTNCATTTTTaaa                                                                                                                                                                   >  3:32989/1‑149 (MQ=255)

                                                                                                                                                                      cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGaaa                                                                                                                                                                                                                                                   >  4:780774/1‑67 (MQ=255)

                                                                                                                                                                      cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAACGCACCCAAAAACAAAGAGACTAAACACGAAGAACACACACCAGATAATCACGAGGAAACAGATCAACACGAGACAAAACAGCAGAAGCAAGACTGGCgcgaca                                   >  4:303423/1‑270 (MQ=255)

                                                                                                                                                                       gAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACaa                                                                                                                                                                >  5:39908/1‑149 (MQ=255)

                                                                                                                                                                        aaaaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtc                                                                                                                                                                                                                                >  8:63393/1‑84 (MQ=255)

                                                                                                                                                                        aaaaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtc                                                                                                                                                                                                                                <  7:63393/84‑1 (MQ=255)

                                                                                                                                                                               gATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGc                                                                                                                                                                                                                 <  5:433906/92‑1 (MQ=255)

                                                                                                                                                                                 tCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAg                                                                                                                                                                                                           <  6:1078039/96‑1 (MQ=255)

                                                                                                                                                                                   aaCACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGt                                                                                                                                                     >  7:742116/1‑148 (MQ=255)

                                                                                                                                                                                   aaCACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGt                                                                                                                                                     <  8:578459/148‑1 (MQ=255)

                                                                                                                                                                                    acacAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAagag                                                                                                                        >  4:297982/1‑176 (MQ=255)

                                                                                                                                                                                      acaAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtct                                                                                                                                                                                                                               >  5:343732/1‑71 (MQ=255)

                                                                                                                                                                                       caAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACgg                                                                                                                                                >  5:586495/1‑149 (MQ=255)

                                                                                                                                                                                       caAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACg                                                                                                                                                 >  7:896847/1‑148 (MQ=255)

                                                                                                                                                                                        aaaTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGa                                                                                                                                               >  6:2613/1‑149 (MQ=255)

                                                                                                                                                                                        aaaTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTa                                                                                                                    <  2:297982/176‑1 (MQ=255)

                                                                                                                                                                                         aaTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtc                                                                                                                                                                                                                                <  5:108198/67‑1 (MQ=255)

                                                                                                                                                                                          aTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGACAATCACGGGGCAAAACACAAAGAGCAATCCTGGCGTGGCAACCATGC‑CTACCTGGAaaca              >  4:668602/1‑275 (MQ=255)

                                                                                                                                                                                          aTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACAAGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAAAAACAGGAGCAAGCCTGGCGGGGCAAAC‑TGGGCCACCAGGAaaca              >  4:115051/1‑275 (MQ=255)

                                                                                                                                                                                              aaGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACC‑TGCGCTACCTGGACACATTAAACCg      >  1:95094/1‑279 (MQ=255)

                                                                                                                                                                                                gCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCa                                                                                                                                       <  5:133288/149‑1 (MQ=255)

                                                                                                                                                                                                 cccccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCa                                                                                                                                       >  8:364384/1‑148 (MQ=255)

                                                                                                                                                                                                 cccccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCa                                                                                                                                       >  8:427182/1‑148 (MQ=255)

                                                                                                                                                                                                  ccccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAcc                                                                                                                                     >  6:1041920/1‑149 (MQ=255)

                                                                                                                                                                                                  ccccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAANGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGACAGATAATCACGAANAAACAAATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGCGNCNACA‑TGCACCACCTGGACACATTaaaaccacct  >  3:53101/1‑272 (MQ=255)

                                                                                                                                                                                                   cccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAccc                                                                                                                                    >  6:172373/1‑149 (MQ=255)

                                                                                                                                                                                                   cccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAccc                                                                                                                                    <  6:300800/149‑1 (MQ=255)

                                                                                                                                                                                                   cccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAccc                                                                                                                                    >  5:58833/1‑149 (MQ=255)

                                                                                                                                                                                                    ccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGaa                                                                                                                           >  3:208597/1‑157 (MQ=255)

                                                                                                                                                                                                        aaTGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCaaaa                                                                                                                                <  8:241901/148‑1 (MQ=255)

                                                                                                                                                                                                          tgctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCAc                                                                     >  4:475341/1‑205 (MQ=255)

                                                                                                                                                                                                            ttgcAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGaa                                                                                                                           <  5:1041920/148‑1 (MQ=255)

                                                                                                                                                                                                            ctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGa                                                                                                                            <  7:685566/148‑1 (MQ=255)

                                                                                                                                                                                                             tgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGaa                                                                                                                           >  7:247444/1‑148 (MQ=255)

                                                                                                                                                                                                              gcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAgg                                                                 <  2:475341/205‑1 (MQ=255)

                                                                                                                                                                                                                aaCATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAagag                                                                                                                        <  8:298633/148‑1 (MQ=255)

                                                                                                                                                                                                                aaCATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAgaga                                                                                                                       <  5:172373/149‑1 (MQ=255)

                                                                                                                                                                                                                aaCATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGACATGCTAGCAGTCGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAagag                                                                                                                        <  7:1058442/148‑1 (MQ=255)

                                                                                                                                                                                                                  cATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAac                                                                                                                 >  2:83221/1‑153 (MQ=255)

                                                                                                                                                                                                                    tGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTACACAAAGTTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTaa                                                                                                                   <  5:820282/149‑1 (MQ=255)

                                                                                                                                                                                                                    tGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAag                                                                                                                          >  2:273815/1‑142 (MQ=255)

TTTGTTTTGTGTGGTTTAACTATCACCTGATAAGAAACCTGTATTCAATTTGCCGGATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACTAAACACGAAGAACACACGCCAGATAATCACGAGGAAACAGATCATCACGAGGCAAAACAGCAGGAGCAAGCCTGGCGTGGCAACC‑TGCGCTACCTGGACACATTAAACCGACTT  >  NC_000913/2560117‑2560589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`