Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,339,818 Δ1 bp 6.7% coding (248/810 nt) mlaF ← ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,339,8180A. 6.7% 422.9 / 21.4 120coding (248/810 nt)mlaFABC transporter maintaining OM lipid asymmetry, ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (60/52);  new base . (4/4);  total (64/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

Only 100 of 122 total aligned reads displayed.

CGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCC  >  NC_000913/3339672‑3339966
                                                                                                                                                  |                                                                                                                                                     
cGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACTTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCAc                                                                                                                                                      <  3:555299/148‑1 (MQ=255)
cGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCAc                                                                                                                                                      <  3:235670/148‑1 (MQ=255)
     tCCAGCTTCATTATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGtata                                                                                                                                                >  1:798990/1‑149 (MQ=255)
     tCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGCACTGtata                                                                                                                                                >  1:9751/1‑149 (MQ=255)
      ccAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGtata                                                                                                                                                >  4:337538/1‑148 (MQ=255)
      ccAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATAc                                                                                                                                               <  2:603700/149‑1 (MQ=255)
       cAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACa                                                                                                                                              >  1:461365/1‑149 (MQ=255)
        aGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACa                                                                                                                                              >  3:769213/1‑148 (MQ=255)
          cTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGgc                                                                                                                                          <  2:821614/149‑1 (MQ=255)
           ttCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGgcg                                                                                                                                         >  2:632228/1‑149 (MQ=255)
           ttCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGgc                                                                                                                                          >  3:290491/1‑148 (MQ=255)
               tcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGa                                                                                                                                      <  3:160070/148‑1 (MQ=255)
                catcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGaa                                                                                                                                     <  3:527976/148‑1 (MQ=255)
                catcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGaa                                                                                                                                     <  4:750371/148‑1 (MQ=255)
                  tcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACga                                                                                                                                  <  1:552741/149‑1 (MQ=255)
                  tcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACg                                                                                                                                   <  4:307227/148‑1 (MQ=255)
                    aCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACgaga                                                                                                                                >  2:594100/1‑149 (MQ=255)
                    aCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGCCTGTATACAGTGCGCAAACggat                                                                                                                                <  2:796097/149‑4 (MQ=255)
                      cGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGAc                                                                                                                               >  3:364453/1‑148 (MQ=255)
                          aaaTGCCACAATGGCGCCGGCAGTTTTGTATGTTCGCGCAATCGATAGGCGGCGATGTCAAAAACTTTCATAGCAGTGAACAACGCCCCGGACTGAAAAATCATTGTCATCCGTTTGCGCACTGTATCCCGGCGCGAACGCGACACCgt                                                                                                                          <  2:502132/147‑2 (MQ=255)
                            aTGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGcc                                                                                                                         <  4:729432/148‑1 (MQ=255)
                                aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGaa                                                                                                                     <  1:386495/148‑1 (MQ=255)
                                aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGaa                                                                                                                     >  2:386495/1‑148 (MQ=255)
                                 acaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAat                                                                                                                    <  4:107821/148‑1 (MQ=255)
                                     tGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATtct                                                                                                               <  2:662059/149‑1 (MQ=255)
                                      ggCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATtct                                                                                                               <  3:961426/148‑1 (MQ=255)
                                                 ttGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa                                                                                                   <  1:616882/149‑1 (MQ=255)
                                                     gTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAt                                                                                               <  1:843812/149‑1 (MQ=255)
                                                         gTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCa                                                                                           >  2:919605/1‑149 (MQ=255)
                                                          ttCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCa                                                                                           >  3:353361/1‑148 (MQ=255)
                                                           tCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCAc                                                                                          >  4:1009891/1‑148 (MQ=255)
                                                               gcAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGa                                                                                     <  2:1009781/149‑1 (MQ=255)
                                                                 aGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGAt                                                                                    <  3:75921/148‑1 (MQ=255)
                                                                    ggATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTgg                                                                                <  2:1060175/149‑1 (MQ=255)
                                                                     gATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGt                                                                               >  1:203546/1‑149 (MQ=255)
                                                                      aTAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGt                                                                               >  3:1008968/1‑148 (MQ=255)
                                                                      aTAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTg                                                                              <  2:882768/149‑1 (MQ=255)
                                                                       tAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGc                                                                             <  2:44734/149‑1 (MQ=255)
                                                                       tAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGc                                                                             >  1:248820/1‑149 (MQ=255)
                                                                          gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGa                                                                           <  4:409746/148‑1 (MQ=255)
                                                                          gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt                                                                          <  2:391106/149‑1 (MQ=255)
                                                                           cGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt                                                                          >  4:671415/1‑148 (MQ=255)
                                                                            gACGTTGTCAAATACGTTCATATCAGAGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATAGTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAtt                                                                         <  4:735465/148‑1 (MQ=255)
                                                                                ttGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGccc                                                                    <  2:1018985/149‑1 (MQ=255)
                                                                                ttGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGcc                                                                     <  3:337538/148‑1 (MQ=255)
                                                                                   tCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGc                                                                  <  3:84738/148‑1 (MQ=255)
                                                                                    cAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGcc                                                                 >  3:328529/1‑148 (MQ=255)
                                                                                       aTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAAt                                                              >  4:14725/1‑148 (MQ=255)
                                                                                         aCGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCa                                                            >  3:430182/1‑148 (MQ=255)
                                                                                           gTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCg                                                                                                                        >  2:458753/1‑86 (MQ=255)
                                                                                           gTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCg                                                                                                                        <  1:458753/86‑1 (MQ=255)
                                                                                           gTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGa                                                          <  4:290491/148‑1 (MQ=255)
                                                                                            ttCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAGTATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGAc                                                         >  3:733038/1‑148 (MQ=255)
                                                                                                tatCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGt                                                    >  1:516609/1‑149 (MQ=255)
                                                                                                tatCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGCCTGTATACAGTGCGCAAACgga                                                                                                                                 >  2:1063353/1‑70 (MQ=21)
                                                                                                tatCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGCCTGTATACAGTGCGCAAACgga                                                                                                                                 <  1:1063353/72‑3 (MQ=21)
                                                                                                 atCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAtt                                                                         >  3:576512/1‑127 (MQ=255)
                                                                                                 atCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAtt                                                                         <  4:576512/127‑1 (MQ=255)
                                                                                                    aGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcg                                                >  1:809051/1‑149 (MQ=255)
                                                                                                     gTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgt                                               >  1:479632/1‑149 (MQ=255)
                                                                                                      tGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtc                                              >  1:141302/1‑149 (MQ=255)
                                                                                                        aacaacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtcgt                                            >  1:758864/1‑149 (MQ=255)
                                                                                                         acaacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtcgt                                            >  4:463290/1‑148 (MQ=255)
                                                                                                         acaacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGtt                                           <  2:479632/149‑1 (MQ=255)
                                                                                                          caacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGtt                                           >  1:717593/1‑148 (MQ=255)
                                                                                                          caacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGtt                                           <  2:717593/148‑1 (MQ=255)
                                                                                                          caacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGtt                                           <  3:1009891/148‑1 (MQ=255)
                                                                                                           aacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGtttt                                         >  2:829078/1‑149 (MQ=255)
                                                                                                            acGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGtttt                                         >  3:744473/1‑148 (MQ=255)
                                                                                                             cGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTa                                        <  4:766289/148‑1 (MQ=255)
                                                                                                             cGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTa                                        <  4:1008968/148‑1 (MQ=255)
                                                                                                                cccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCa                                                                                        >  1:176963/1‑97 (MQ=255)
                                                                                                                cccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCa                                                                                        <  2:176963/97‑1 (MQ=255)
                                                                                                                cccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGa                                    <  2:980714/149‑1 (MQ=255)
                                                                                                                 ccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGa                                    >  3:84943/1‑148 (MQ=255)
                                                                                                                 ccGGACCGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGAt                                   >  2:679444/1‑149 (MQ=255)
                                                                                                                     aCTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGccc                               >  2:231868/1‑149 (MQ=255)
                                                                                                                      cTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGccc                               >  3:698445/1‑148 (MQ=255)
                                                                                                                        gAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCGGCCAATCAGACGGATTAGCGTCGTTTTACCGATGCCCGa                             >  3:697443/1‑148 (MQ=255)
                                                                                                                        gAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGa                                    >  3:795612/1‑141 (MQ=255)
                                                                                                                        gAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGa                                    <  4:795612/141‑1 (MQ=255)
                                                                                                                          aaTAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATgg                          >  1:537025/1‑149 (MQ=255)
                                                                                                                          aaTAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATgg                          >  1:46830/1‑149 (MQ=255)
                                                                                                                           aTAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATgg                          >  4:718211/1‑148 (MQ=255)
                                                                                                                                 tGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCAt                    >  3:731905/1‑148 (MQ=255)
                                                                                                                                    tCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATc                >  2:313474/1‑149 (MQ=255)
                                                                                                                                      aTCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGc              >  1:946157/1‑149 (MQ=255)
                                                                                                                                       tCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCt                                                                                  >  4:278393/1‑80 (MQ=255)
                                                                                                                                       tCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCt                                                                                  <  3:278393/80‑1 (MQ=255)
                                                                                                                                        tcGTTCGCGCCTGTATACAGTGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGa                  <  2:568613/142‑1 (MQ=255)
                                                                                                                                         cGTTCGCGCCTGTATACAGTGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtcg                                             <  3:25289/115‑1 (MQ=255)
                                                                                                                                         cGTTCGCGCCTGTATACAGTGCGCAAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCg            >  3:601156/1‑148 (MQ=255)
                                                                                                                                          gTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgt                                               <  3:79013/112‑1 (MQ=255)
                                                                                                                                          gTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgt                                               >  4:79013/1‑112 (MQ=255)
                                                                                                                                          gTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTg          >  2:908851/1‑149 (MQ=255)
                                                                                                                                           tttGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGa                                    <  3:902739/122‑1 (MQ=255)
                                                                                                                                           tttGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGa                                    >  4:902739/1‑122 (MQ=255)
                                                                                                                                           tttGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTg          >  4:622655/1‑148 (MQ=255)
                                                                                                                                                 cACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTc    >  3:935518/1‑148 (MQ=255)
                                                                                                                                                 cACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTc    <  4:84943/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCC  >  NC_000913/3339672‑3339966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: