Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,121,588:1 +C 6.7% intergenic (‑34/+21) wcaJ ← / ← cpsG colanic biosynthesis UDP‑glucose lipid carrier transferase/phosphomannomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,121,5881.C 6.7% 452.8 / 25.3 105intergenic (‑34/+21)wcaJ/cpsGcolanic biosynthesis UDP‑glucose lipid carrier transferase/phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base . (48/50);  new base C (5/2);  total (53/52)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGCCAGTC  >  NC_000913/2121441‑2121734
                                                                                                                                                    |                                                                                                                                                  
ttCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTg                                                                                                                                                     <  4:432206/148‑1 (MQ=255)
 tCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTgg                                                                                                                                                   <  3:825704/148‑1 (MQ=255)
  cGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTggg                                                                                                                                                  >  4:7120/1‑148 (MQ=255)
   gCAAACCAACCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGt                                                                                                                                                <  1:9409/149‑1 (MQ=255)
     aaaCCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTa                                                                                                                                               <  4:123368/148‑1 (MQ=255)
      aaCCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTaa                                                                                                                                              >  4:937264/1‑148 (MQ=255)
      aaCCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAg                                                                                                                                             <  1:253806/149‑1 (MQ=255)
         cAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAggg                                                                                                                                           <  3:222222/148‑1 (MQ=255)
         cAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGa                                                                                                                                          >  1:74824/1‑149 (MQ=255)
          aGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGa                                                                                                                                          >  4:194394/1‑148 (MQ=255)
           gCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGaa                                                                                                                                         >  3:768270/1‑148 (MQ=255)
           gCCATACTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAg                                                                                                                                        >  2:525622/1‑149 (MQ=255)
             cATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGAt                                                                                                                                      >  1:633445/1‑149 (MQ=255)
             cATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGAt                                                                                                                                      <  2:149259/149‑1 (MQ=255)
              aTAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATc                                                                                                                                     <  1:577594/149‑1 (MQ=255)
                 gTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCg                                                                                                                                   <  3:60524/148‑1 (MQ=255)
                  tCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGa                                                                                                                                  >  3:1041515/1‑148 (MQ=255)
                      gCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCg                                                                                                                                   >  3:447388/1‑143 (MQ=255)
                      gCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCg                                                                                                                                   <  4:447388/143‑1 (MQ=255)
                       cAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTAc                                                                                                                            <  2:619228/149‑1 (MQ=255)
                         aaCATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCg                                                                                                                                   <  4:577742/140‑1 (MQ=255)
                         aaCATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCg                                                                                                                                   >  3:577742/1‑140 (MQ=255)
                         aaCATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTc                                                                                                                          >  1:905043/1‑149 (MQ=255)
                             tgatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGtt                                                                                                                       <  4:768270/148‑1 (MQ=255)
                              gatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCa                                                                                                                     <  2:748690/149‑1 (MQ=255)
                              gatgGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCa                                                                                                                     <  1:525622/149‑1 (MQ=255)
                                 ggTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCa                                                                                                                  >  1:1007964/1‑149 (MQ=255)
                                   tGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGATATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAAc                                                                                                                <  2:894368/149‑1 (MQ=255)
                                       atCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTca                                                                                                            >  2:365855/1‑149 (MQ=255)
                                        tCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGTCATTACTCGTTCAGCCACGTcag                                                                                                           <  2:858472/149‑1 (MQ=255)
                                        tCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTcag                                                                                                           <  2:905043/149‑1 (MQ=255)
                                           gAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGa                                                                                                                                  <  3:965617/123‑1 (MQ=255)
                                           gAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGa                                                                                                                                  >  4:965617/1‑123 (MQ=255)
                                           gAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCCACTGCGGGTAAggg                                                                                                                                           >  4:144297/1‑114 (MQ=255)
                                           gAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCCACTGCGGGTAAggg                                                                                                                                           <  3:144297/114‑1 (MQ=255)
                                            aaaaGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCATACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCaga                                                                                                       <  2:791377/149‑1 (MQ=255)
                                             aaaGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCagag                                                                                                      >  2:596970/1‑149 (MQ=255)
                                               aGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgt                                                                                                     >  3:732171/1‑148 (MQ=255)
                                                    tGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgcgt                                                                                               <  2:962636/149‑1 (MQ=255)
                                                    tGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgcg                                                                                                <  4:14521/148‑1 (MQ=255)
                                                        ccATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgcgttcgc                                                                                           >  1:522618/1‑149 (MQ=255)
                                                         cATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgcgttcgc                                                                                           >  3:1004120/1‑148 (MQ=255)
                                                           tAGAGATTAACGCTGCATTGGCTTTCGCTCGCTCGCGCTTTTTTAGATTTATCATCGTTGTTCGTGTTATGAGCCCCTTACCCGCAGTGGGGTAAGCGAAGATCCGACATCACTCGTTCAGCAACGTCAGCAGATTACGCGTTCGCGCt                                                                                        >  2:81113/1‑149 (MQ=255)
                                                           tAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCTTCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCgcgc                                                                                         >  3:249609/1‑148 (MQ=255)
                                                             gagaTTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTc                                                                                      <  2:18771/149‑1 (MQ=255)
                                                               gaTTAACGGTGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTcc                                                                                     <  3:656468/148‑1 (MQ=255)
                                                                aTTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCa                                                                                    <  4:1004120/148‑1 (MQ=255)
                                                                  tAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATc                                                                                  <  4:391825/148‑1 (MQ=255)
                                                                        tGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGc                                                                            >  4:865298/1‑148 (MQ=255)
                                                                           aTTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgcgttc                                                                                             <  1:894747/128‑1 (MQ=255)
                                                                           aTTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgttcgcgttc                                                                                             >  2:894747/1‑128 (MQ=255)
                                                                            ttGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACAt                                                                        >  4:880673/1‑148 (MQ=255)
                                                                             tGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATc                                                                       >  3:36330/1‑148 (MQ=255)
                                                                               gTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCAcc                                                                    >  1:1037360/1‑149 (MQ=255)
                                                                                     gctcgctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACcgcgcg                                                               >  4:916209/1‑148 (MQ=255)
                                                                                     gctcgctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGa                                                              <  2:672166/149‑1 (MQ=255)
                                                                                       tcgctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGAt                                                             >  4:197856/1‑148 (MQ=255)
                                                                                         gctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTc                                                           <  4:261899/148‑1 (MQ=255)
                                                                                         gctcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTc                                                           <  4:758454/148‑1 (MQ=255)
                                                                                           tcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgt                                                                                                     <  1:949353/104‑1 (MQ=255)
                                                                                           tcgcGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAgt                                                                                                     >  2:949353/1‑104 (MQ=255)
                                                                                            cgcgCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCCACTGCGGGTAAgg                                                                                                                                            >  3:443769/1‑64 (MQ=25)
                                                                                            cgcgCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCCACTGCGGGTAAgg                                                                                                                                            <  4:443769/64‑1 (MQ=25)
                                                                                              cgcTTTTTAAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACAtt                                                     <  2:927049/149‑1 (MQ=255)
                                                                                               gcTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACAtt                                                     <  4:36330/148‑1 (MQ=255)
                                                                                                 ttttttAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAg                                                  >  2:410387/1‑149 (MQ=255)
                                                                                                         aTTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGcacc                                           >  4:391811/1‑148 (MQ=255)
                                                                                                          tttGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGcacca                                          >  3:735991/1‑148 (MQ=255)
                                                                                                          tttGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGcacca                                          >  3:751316/1‑148 (MQ=255)
                                                                                                               cATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCATAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCATGCGCACCACCGGtt                                    <  1:807407/149‑1 (MQ=255)
                                                                                                                aTCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTc                                   <  2:522618/149‑1 (MQ=255)
                                                                                                                aTCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTc                                   >  2:938405/1‑149 (MQ=255)
                                                                                                                   gttgttCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGt                                >  2:587997/1‑149 (MQ=255)
                                                                                                                     tgttCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTa                               >  4:599703/1‑148 (MQ=255)
                                                                                                                       ttCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTg                            <  2:404048/149‑1 (MQ=255)
                                                                                                                        tCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTgg                           <  1:496304/149‑1 (MQ=255)
                                                                                                                        tCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTgg                           <  1:155531/149‑1 (MQ=255)
                                                                                                                          cTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCTCCGGTTCGGTATTGGa                          <  4:403715/148‑1 (MQ=255)
                                                                                                                           tGTTATTGGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCagag                                                                                                      >  2:574575/1‑71 (MQ=255)
                                                                                                                           tGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAgg                        >  1:49880/1‑149 (MQ=255)
                                                                                                                           tGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAgg                        >  1:1029287/1‑149 (MQ=255)
                                                                                                                            gTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGt                       <  1:48019/149‑1 (MQ=255)
                                                                                                                             ttattaGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTg                      >  2:320093/1‑149 (MQ=255)
                                                                                                                              tattaGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTg                      >  4:301455/1‑148 (MQ=255)
                                                                                                                                  aGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCa                  <  3:865298/148‑1 (MQ=255)
                                                                                                                                   ttcccTTACCCCACTGCGGGTAAGGGGAGGTCCGACTTTACTCGTTCAGCAACGTCAGCTGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGGGCGATTCCAGATTCAGGCGCACCAACGGTTCGGTATTGGAGGTGCGCAgg                >  1:360635/3‑149 (MQ=255)
                                                                                                                                    tcccTTACCCCACTGCGGGTAAGGGGAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAgg                >  4:934455/2‑148 (MQ=255)
                                                                                                                                    tcccTTACCCCACTGCGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAgg                >  4:11759/2‑148 (MQ=255)
                                                                                                                                        ttACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTaa            <  3:891198/148‑1 (MQ=255)
                                                                                                                                         tACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCg                                                                              >  4:961787/1‑81 (MQ=255)
                                                                                                                                         tACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCg                                                                              <  3:961787/81‑1 (MQ=255)
                                                                                                                                         tACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGTGCAGGTTAAAg          <  1:560876/149‑1 (MQ=255)
                                                                                                                                         tACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTaaa           >  3:1030803/1‑148 (MQ=255)
                                                                                                                                          aCCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGGGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGt                       >  1:681429/1‑135 (MQ=255)
                                                                                                                                          aCCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGGGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGt                       <  2:681429/135‑1 (MQ=255)
                                                                                                                                          aCCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAgc         <  1:938405/149‑1 (MQ=255)
                                                                                                                                             cGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAgcgc       <  3:542024/148‑1 (MQ=255)
                                                                                                                                             cGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAgcgc       >  4:545010/1‑148 (MQ=255)
                                                                                                                                             cGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAgcgc       >  4:1050244/1‑148 (MQ=255)
                                                                                                                                                  tGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGCCAGTc  <  4:768609/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTCGCAAACCAGCCATAGTCCGGCAAACATGATGGTGATATCTGAAAAGCGTTGCACCATAGAGATTAACGATGCATTGGTTTTCGCTCGCTCGCGCTTTTTTAGATTTGTCATCGTTGTTCCTGTTATTAGCCCCTTACCCGCAGTGGGGTAAGGGAAGATCCGACATTACTCGTTCAGCAACGTCAGCAGAGTTCGCGTTCGCGCTTCCATCAGCGGCACATCACCGCGCGATTCCACATTCAGGCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGCCAGTC  >  NC_000913/2121441‑2121734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: