Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,295,809:1 +T 7.0% intergenic (+15/+627) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,295,8091.T 7.0% 402.8 / 27.3 100intergenic (+15/+627)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (37/56);  new base T (3/4);  total (40/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-01

Only 100 of 108 total aligned reads displayed.

CTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATG  >  NC_000913/4295661‑4295955
                                                                                                                                                     |                                                                                                                                                   
gttaagcgtcgcatcaggcgttgatgtcggatgcggcataaacgccttatccgacctacaaaatcacgctaaatcaggctattgcgctgtctggcgtaggcctgataagacgcggcaagcgtcgcatcaggcattcacacATTGCCggg                                                                                                                                                      <  1:428411‑M1/9‑1 (MQ=255)
 ttaagcgtcgcatcaggcgttgatgtcggatgcggcataaacgccttatccgacctacaaaatcacgctaaatcaggctattgcgctgtctggcgtaggcctgataagacgcggcaagcgtcgcatcaggcattcacacATTGCCggg                                                                                                                                                      >  3:22139‑M1/140‑148 (MQ=255)
  gcgcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCggg‑‑g                                                                                                                                                   <  3:26930/148‑1 (MQ=255)
  gcgcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑Ga                                                                                                                                                  <  1:387720/149‑1 (MQ=255)
    gcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑Gat                                                                                                                                                 <  4:600530/148‑1 (MQ=255)
          cGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCAc                                                                                                                                           <  4:899428/148‑1 (MQ=255)
              caggcgttgatgtcggatgcggcataaacgccttatccgacctacaaaatcacgctaaatcaggctattgcgctgtctggcgtaggcctgataagacgcggcaagcgtcgcatcaggcattcacacATTGCCGGG‑‑GATATCCACCCgg                                                                                                                                       <  4:15169‑M1/22‑1 (MQ=255)
                 gcgttgatgtcggatgcggcataaacgccttatccgacctacaaaatcacgctaaatcaggctattgcgctgtctggcgtaggcctgataagacgcggcaagcgtcgcatcaggcattcacacATTGCCGGG‑‑GATATCCACCCGGCaa                                                                                                                                    >  3:15169‑M1/124‑148 (MQ=255)
                  gTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAAt                                                                                                                                   >  4:55641/1‑148 (MQ=255)
                      tGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCggg‑‑g                                                                                                                                                   <  1:867223/128‑1 (MQ=255)
                      tGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCggg‑‑g                                                                                                                                                   >  2:867223/1‑128 (MQ=255)
                      tGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAAtgtgtg                                                                                                                              <  2:869694/149‑1 (MQ=255)
                       gcgcTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAAtgtgtg                                                                                                                              <  3:49380/148‑1 (MQ=255)
                        cgcTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGaa                                                                                                                            <  2:472448/149‑1 (MQ=255)
                        cgcTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGa                                                                                                                             <  4:429529/148‑1 (MQ=255)
                           tGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATg                                                                                                                          <  4:573199/148‑1 (MQ=255)
                               ggTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGa                                                                                                                     <  2:279041/149‑1 (MQ=255)
                                     ggTGAATGCAAAGTAGGAACACAAATTTGACAGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCTGG‑‑GATATCCATCTGGCAATGTGTGAATGCCTGATGCTAc                                                                                                               <  2:453294/149‑1 (MQ=255)
                                      gTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCGGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACg                                                                                                              >  1:742494/1‑149 (MQ=255)
                                       tGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACg                                                                                                              <  4:740199/148‑1 (MQ=255)
                                          ttGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCtt                                                                                                           >  3:795572/1‑148 (MQ=255)
                                            gCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGcc                                                                                                        >  2:512950/1‑149 (MQ=255)
                                            gCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGc                                                                                                         <  4:397052/148‑1 (MQ=255)
                                             ccAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGcc                                                                                                        >  4:708999/1‑148 (MQ=255)
                                                aGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCcgcg                                                                                                     <  4:983879/148‑1 (MQ=255)
                                                 gTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTc                                                                                                   <  1:803761/149‑1 (MQ=255)
                                                 gTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTc                                                                                                   <  1:707872/149‑1 (MQ=255)
                                                  tGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAAtgtg                                                                                                                                >  3:669539/1‑119 (MQ=255)
                                                  tGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGa                                                                                                                     <  3:643311/130‑1 (MQ=255)
                                                  tGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGa                                                                                                                     >  4:643311/1‑130 (MQ=255)
                                                        cacaAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCggg                                                                                                                                                      <  3:29616/93‑1 (MQ=255)
                                                        cacaAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCggg                                                                                                                                                      >  4:29616/1‑93 (MQ=255)
                                                        cacaAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                             <  4:644830/148‑1 (MQ=255)
                                                         acaAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                           <  2:946599/149‑1 (MQ=255)
                                                         acaAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                           >  1:506111/1‑149 (MQ=255)
                                                          caAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                           >  4:841682/1‑148 (MQ=255)
                                                          caAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                          <  2:700619/149‑1 (MQ=255)
                                                            aaTTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                                        >  2:29443/1‑149 (MQ=255)
                                                              tttGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                                                      >  1:715634/1‑149 (MQ=255)
                                                                  aCCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTAATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc                                                                                   <  4:962958/148‑1 (MQ=255)
                                                                   ccGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCAACCGGCAATGTGTGCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCGTACGCCa                                                                                  <  4:714448/148‑1 (MQ=255)
                                                                         gAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcg                                                                           >  1:280177/1‑149 (MQ=255)
                                                                         gAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcg                                                                           >  2:479937/1‑149 (MQ=255)
                                                                          aaaGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcg                                                                           >  4:23549/1‑148 (MQ=255)
                                                                              cgcTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAt                                                                       <  3:918988/148‑1 (MQ=255)
                                                                              cgcTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa                                                                      <  1:603471/149‑1 (MQ=255)
                                                                                 tGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGTGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGGGCAATAGcc                                                                   <  1:924894/149‑1 (MQ=255)
                                                                                 tGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc                                                                   <  2:691560/149‑1 (MQ=255)
                                                                                 tGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                    <  3:841682/148‑1 (MQ=255)
                                                                                 tGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                    <  4:477102/148‑1 (MQ=255)
                                                                                   gCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAt                                                                       >  4:211716/1‑143 (MQ=255)
                                                                                    cTTATGAGGGTGAAGTGCTGGGCAAATGTGATAAAACTGCGGATCGATAAGTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCAGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                                                 <  3:23448/148‑1 (MQ=37)
                                                                                     ttATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAt                                                               >  1:46348/1‑149 (MQ=255)
                                                                                         gAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAg                                                           >  1:804538/1‑149 (MQ=255)
                                                                                          aGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAg                                                           <  3:336849/148‑1 (MQ=255)
                                                                                            cGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGt                                                        <  2:715634/149‑1 (MQ=255)
                                                                                             gTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTg                                                       >  1:754210/1‑149 (MQ=255)
                                                                                                aaGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                               >  1:144383/1‑106 (MQ=255)
                                                                                                aaGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                               <  2:144383/106‑1 (MQ=255)
                                                                                                  gTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt                                                  >  1:940600/1‑149 (MQ=255)
                                                                                                  gTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt                                                  <  1:1038835/149‑1 (MQ=255)
                                                                                                   tGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAAtg                                                                                                                                  >  3:36306/1‑68 (MQ=255)
                                                                                                   tGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAAtg                                                                                                                                  <  4:36306/68‑1 (MQ=255)
                                                                                                     cTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                <  3:23549/148‑1 (MQ=255)
                                                                                                     cTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                <  3:988355/148‑1 (MQ=255)
                                                                                                     cTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                                               <  2:280177/149‑1 (MQ=255)
                                                                                                     cTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                                               <  1:479937/149‑1 (MQ=255)
                                                                                                              tttGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATa                                      >  2:268090/1‑149 (MQ=255)
                                                                                                               ttGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATa                                      >  3:527956/1‑148 (MQ=255)
                                                                                                               ttGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATa                                      >  3:744315/1‑148 (MQ=255)
                                                                                                                tGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATaa                                     <  4:784694/148‑1 (MQ=255)
                                                                                                                   tAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                 <  4:1026950/85‑1 (MQ=255)
                                                                                                                   tAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                 >  3:1026950/1‑85 (MQ=255)
                                                                                                                    aaaaCTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGt                                <  2:46348/149‑1 (MQ=255)
                                                                                                                       aCTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGttt                              >  3:133125/1‑148 (MQ=255)
                                                                                                                         tGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt                            <  3:625679/148‑1 (MQ=255)
                                                                                                                           cGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGc                          <  3:124155/148‑1 (MQ=255)
                                                                                                                             gATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGc                       >  1:296647/1‑149 (MQ=255)
                                                                                                                                cAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCAt                     <  3:160190/148‑1 (MQ=255)
                                                                                                                                 aataatTGAAGATTGCCGGG‑‑GATATCCACCCGGCGATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATcc                   >  2:817468/1‑149 (MQ=255)
                                                                                                                                 aataatTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATcc                   >  1:717301/1‑149 (MQ=255)
                                                                                                                                  ataatTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATcc                   >  4:798252/1‑148 (MQ=255)
                                                                                                                                   taatTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCg                  >  3:92225/1‑148 (MQ=255)
                                                                                                                                     ttcacacATTGCCGGGTGGATATCC‑‑CCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATGAGGCGTTTAt                            <  4:989645‑M1/129‑1 (MQ=255)
                                                                                                                                     ttcacacATTGCCGGGTGGATATCC‑‑CCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATGAGGCGTTTAt                            >  3:989645‑M1/8‑136 (MQ=255)
                                                                                                                                     atTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGAc                <  4:527956/148‑1 (MQ=255)
                                                                                                                                      ttGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACAt              >  1:381969/1‑149 (MQ=255)
                                                                                                                                       cacacATTGCCGGGTGGATATCC‑‑CCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAg                                                                                 <  4:519087‑M1/76‑1 (MQ=255)
                                                                                                                                       cacacATTGCCGGGTGGATATCC‑‑CCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAg                                                                                 >  3:519087‑M1/6‑81 (MQ=255)
                                                                                                                                         cacATTGCCGGGTGGATATCC‑‑CCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCaa           <  2:700480‑M1/146‑1 (MQ=255)
                                                                                                                                          aGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt                            >  2:348750/1‑131 (MQ=255)
                                                                                                                                          aGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt                            <  1:348750/131‑1 (MQ=255)
                                                                                                                                           cATTGCCGGGTGGATATCC‑‑CCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCgg                                         <  3:1047972‑M1/116‑1 (MQ=255)
                                                                                                                                           cATTGCCGGGTGGATATCC‑‑CCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCgg                                         >  4:1047972‑M1/2‑117 (MQ=255)
                                                                                                                                             ttGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATaa                                     >  4:822625/1‑119 (MQ=255)
                                                                                                                                             ttGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATaa                                     <  3:822625/119‑1 (MQ=255)
                                                                                                                                              tGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCt      <  2:847668/149‑1 (MQ=255)
                                                                                                                                                ccGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTg     <  4:744315/148‑1 (MQ=255)
                                                                                                                                                ccGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGa    <  1:553730/149‑1 (MQ=255)
                                                                                                                                                  ggg‑‑gATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATg  >  2:633374/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGG‑‑GATATCCACCCGGCAATGTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATG  >  NC_000913/4295661‑4295955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: