Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 847,215 A→T 8.0% intergenic (‑96/+43) glnP ← / ← glnH glutamine transporter subunit/glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913847,2150AT 8.0% 415.5 / 18.0 123intergenic (‑96/+43)glnP/glnHglutamine transporter subunit/glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (58/55);  new base T (3/7);  total (61/62)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 124 total aligned reads displayed.

CCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCT  >  NC_000913/847067‑847362
                                                                                                                                                    |                                                                                                                                                    
ccTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATa                                                                                                                                                      <  1:439336/149‑1 (MQ=255)
       tCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGgaga                                                                                                                                              <  2:802517/149‑1 (MQ=255)
        cAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGgaga                                                                                                                                              <  4:738740/148‑1 (MQ=255)
        cAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGgaga                                                                                                                                              <  4:110896/148‑1 (MQ=255)
        cAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGagag                                                                                                                                             <  2:80771/149‑1 (MQ=255)
        cAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGagag                                                                                                                                             <  2:290015/149‑1 (MQ=255)
        cAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGagag                                                                                                                                             <  1:484311/149‑1 (MQ=255)
         aGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTCATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGGAGAgg                                                                                                                                            <  2:879838/149‑1 (MQ=255)
          gAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGGAGAgga                                                                                                                                           <  1:1061281/149‑2 (MQ=255)
          gAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGGAGAgga                                                                                                                                           <  2:485484/149‑2 (MQ=255)
             gCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCTATGGGGAGAggatt                                                                                                                                         <  4:736363/148‑4 (MQ=255)
              cGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTACTTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAACCCTCTCCCCTATGGGGAGAggatta                                                                                                                                        <  4:609060/148‑5 (MQ=255)
                gAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGc                                                                                                                                     >  1:198918/1‑149 (MQ=255)
                         gCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGtt                                                                                                                            >  1:396844/1‑149 (MQ=255)
                           cAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAAc                                                                                                                                        <  2:844426/135‑1 (MQ=255)
                           cAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAAc                                                                                                                                        >  1:844426/1‑135 (MQ=255)
                            aGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGtttt                                                                                                                          >  3:505009/1‑148 (MQ=255)
                            aGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGCGCGtttt                                                                                                                          <  4:91525/148‑1 (MQ=255)
                              aTGGCACTCCGGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGa                                                                                                                                          >  3:845719/1‑130 (MQ=255)
                                   aCTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGgtg                                                                                                                   <  4:413645/148‑1 (MQ=255)
                                    cTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGgtgt                                                                                                                  <  3:449450/148‑1 (MQ=255)
                                     tCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACa                                                                                                                       >  1:643138/1‑142 (MQ=255)
                                     tCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACa                                                                                                                       <  2:643138/142‑1 (MQ=255)
                                      ccAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAg                                                                                                                <  3:37421/148‑1 (MQ=255)
                                         gTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGtt                                                                                                            <  1:273255/149‑1 (MQ=255)
                                         gTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGt                                                                                                             >  3:407972/1‑148 (MQ=255)
                                          tCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAACCCTCTCCCCTATGGGGAGAggattagg                                                                                                                                      <  1:802156/122‑7 (MQ=255)
                                           cAAACTGCATATGTTTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGt                                                                                                             <  4:102732/145‑1 (MQ=255)
                                           cAAACTGCATATGTTTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGt                                                                                                             >  3:102732/1‑145 (MQ=255)
                                              aCTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGATTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGttatta                                                                                                        >  3:680664/1‑148 (MQ=255)
                                               cTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTattatt                                                                                                      >  1:222215/1‑149 (MQ=255)
                                                  cATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCg                                                                                                   <  2:198918/149‑1 (MQ=255)
                                                    tatGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGt                                                                                                 >  2:459417/1‑149 (MQ=255)
                                                        ttgttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCa                                                                                              >  4:1052666/1‑148 (MQ=255)
                                                          gttCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTa                                                                                           >  2:47577/1‑149 (MQ=255)
                                                            tCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGGGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGGGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTCTTATTTCGGTTCAGTAc                                                                                          >  4:895600/1‑148 (MQ=255)
                                                            tCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTAc                                                                                          >  3:771135/1‑148 (MQ=255)
                                                              cTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGa                                                                                       >  2:385120/1‑149 (MQ=255)
                                                              cTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGa                                                                                       >  1:197771/1‑149 (MQ=255)
                                                                gTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAAc                                                                                     >  2:274535/1‑149 (MQ=255)
                                                                    ccGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCAt                                                                                  <  4:53279/148‑1 (MQ=255)
                                                                        ggtgTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCAtttttt                                                                             <  2:680984/149‑1 (MQ=255)
                                                                           gtAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTa                                                                          <  2:843120/149‑1 (MQ=255)
                                                                            tAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAg                                                                         >  1:49941/1‑149 (MQ=255)
                                                                              aataatCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGAt                                                                       >  2:943083/1‑149 (MQ=255)
                                                                               ataatCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGAt                                                                       >  4:475973/1‑148 (MQ=255)
                                                                                   tCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCg                                                                   >  4:392888/1‑148 (MQ=255)
                                                                                   tCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGt                                                                  >  1:665034/1‑149 (MQ=255)
                                                                                   tCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGt                                                                  >  2:212923/1‑149 (MQ=255)
                                                                                   tCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGt                                                                  >  1:1001541/1‑149 (MQ=255)
                                                                                   tCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGt                                                                  >  1:597400/1‑149 (MQ=255)
                                                                                    cAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCTCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGtt                                                                 <  2:79813/149‑1 (MQ=255)
                                                                                        ttCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTa                                                                                                                         <  4:240955/89‑1 (MQ=255)
                                                                                        ttCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTa                                                                                                                         >  3:240955/1‑89 (MQ=255)
                                                                                        ttCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTa                                                              <  3:69864/148‑1 (MQ=255)
                                                                                        ttCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTa                                                              <  4:592756/148‑1 (MQ=255)
                                                                                        ttCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTa                                                              <  4:27896/148‑1 (MQ=255)
                                                                                             ggTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTcc                                                        >  1:278347/1‑149 (MQ=255)
                                                                                             ggTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTcc                                                        >  2:728398/1‑149 (MQ=255)
                                                                                             ggTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTcc                                                        <  2:250564/149‑1 (MQ=255)
                                                                                                aattaatGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGtt                                                     <  1:66865/149‑1 (MQ=255)
                                                                                                 attaatGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTtc                                                    >  2:1078026/1‑149 (MQ=255)
                                                                                                  ttaatGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTtct                                                   >  2:253674/1‑149 (MQ=255)
                                                                                                  ttaatGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTtct                                                   >  1:576398/1‑149 (MQ=255)
                                                                                                   taatGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTtct                                                   <  4:505009/148‑1 (MQ=255)
                                                                                                      tGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTcgcg                                               >  2:40078/1‑149 (MQ=255)
                                                                                                           ccGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGt                                                                  <  4:365246/125‑1 (MQ=255)
                                                                                                           ccGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGt                                                                  >  3:365246/1‑125 (MQ=255)
                                                                                                           ccGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAgg                                           >  4:135762/1‑148 (MQ=255)
                                                                                                           ccGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAgg                                           <  3:658593/148‑1 (MQ=255)
                                                                                                             ggAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGtt                                        >  2:428002/1‑149 (MQ=255)
                                                                                                                gCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTc                                     >  1:439027/1‑149 (MQ=255)
                                                                                                                  gggTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCaa                                   <  1:943083/149‑1 (MQ=255)
                                                                                                                  gggTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCaa                                   <  2:1001541/149‑1 (MQ=255)
                                                                                                                         cgcCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCAttttt                                                                              <  2:237185/99‑1 (MQ=255)
                                                                                                                         cgcCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCAttttt                                                                              >  1:237185/1‑99 (MQ=255)
                                                                                                                           ccccTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGt                           <  3:135762/148‑1 (MQ=255)
                                                                                                                           ccccTCACCCAAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTTTCGCGCAGGGTTTTCAACGCGTCGtt                          <  2:459160/149‑1 (MQ=255)
                                                                                                                             ccTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTg                         <  3:608647/148‑1 (MQ=255)
                                                                                                                              cTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGAc                       <  2:396844/149‑1 (MQ=255)
                                                                                                                                 aCCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACttt                    <  2:987252/149‑1 (MQ=255)
                                                                                                                                    cTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGt                  >  3:33502/1‑148 (MQ=255)
                                                                                                                                     tAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTattatt                                                                                                      >  3:910488/1‑63 (MQ=255)
                                                                                                                                     tAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTattatt                                                                                                      <  4:910488/63‑1 (MQ=255)
                                                                                                                                      aaTCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCa                >  4:116537/1‑148 (MQ=255)
                                                                                                                                         ccTCTCCCCTATGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCa            >  1:985475/1‑149 (MQ=255)
                                                                                                                                          ctctCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAggg                                          >  2:184577/1‑118 (MQ=255)
                                                                                                                                          ctctCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAggg                                          <  1:184577/118‑1 (MQ=255)
                                                                                                                                           tctcCCCTATGGGGGGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCTTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAg           >  4:1012669/1‑148 (MQ=255)
                                                                                                                                           tctcCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCgg                                                                                                  <  4:92353/61‑1 (MQ=255)
                                                                                                                                           tctcCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCgg                                                                                                  >  3:92353/1‑61 (MQ=255)
                                                                                                                                           tctcCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAg           <  3:392888/148‑1 (MQ=255)
                                                                                                                                             tcCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCt         <  4:680664/148‑1 (MQ=255)
                                                                                                                                               cccATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCtcgt      >  1:33344/1‑149 (MQ=255)
                                                                                                                                               cccATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACAGAACCACTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCACGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCCGCtcg       >  4:687304/1‑148 (MQ=255)
                                                                                                                                               cccATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTAGTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCtcgt      >  1:615223/1‑149 (MQ=255)
                                                                                                                                                ccATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCtcgtc     >  1:89401/1‑149 (MQ=255)
                                                                                                                                                ccATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCtcgt      <  4:2695/148‑1 (MQ=255)
                                                                                                                                                 cATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCtcgtcg    >  1:107061/1‑149 (MQ=255)
                                                                                                                                                   tAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCt  >  1:14843/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATCAAATTCAGGGTAATTAATGGCCCCGGAGCGGGTTTGCGCCCCTCACCCTAATCCTCTCCCCATAGGGGAGAGGGAACTGCCAGTGCGTTTTACAGGTGTAGCGTTATTATTTCGGTTCAGTACCGAACCATTTTTTGTAGATTTCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCT  >  NC_000913/847067‑847362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: