Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 983,117 G→A 13.6% G15S (GGT→AGT)  ycbK → M15A protease‑related family periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913983,1170GA13.6% 277.2 / 31.0 103G15S (GGT→AGT) ycbKM15A protease‑related family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (44/45);  new base A (7/7);  total (51/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

Only 100 of 109 total aligned reads displayed.

TGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTCGATGGCAGAGGCTATATTCA  >  NC_000913/982969‑983262
                                                                                                                                                    |                                                                                                                                                 
tgctgGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTtg                                                                                                                                                   <  2:176677/149‑1 (MQ=255)
  ctgGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTtggt                                                                                                                                                 >  1:1050450/1‑149 (MQ=255)
     gggTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGc                                                                                                                                              >  2:175216/1‑149 (MQ=255)
       gTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGt                                                                                                                                            >  1:996449/1‑149 (MQ=255)
       gTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGt                                                                                                                                            <  2:709018/149‑1 (MQ=255)
        ttGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGtt                                                                                                                                           <  2:131341/149‑1 (MQ=255)
        ttGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTAGTGGCGtt                                                                                                                                           <  1:33925/149‑1 (MQ=255)
              cATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACt                                                                                                                                      >  3:266512/1‑148 (MQ=255)
                tCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCgg                                                                                                                                   >  1:291041/1‑149 (MQ=255)
                tCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCgg                                                                                                                                   <  2:198529/149‑1 (MQ=255)
                 cTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGt                                                                                                                                  <  1:442858/149‑1 (MQ=255)
                   tGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTgc                                                                                                                                >  2:390813/1‑149 (MQ=255)
                          tGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGAAGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATc                                                                                                                         >  1:771637/1‑149 (MQ=255)
                             tttACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTAGTGGCGTTGCACTCGGTGCCGCCATCCTg                                                                                                                      >  1:236144/1‑149 (MQ=255)
                               tACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGcc                                                                                                                    <  1:782173/149‑1 (MQ=255)
                                  gggCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGAc                                                                                                                 >  1:143140/1‑149 (MQ=255)
                                       gTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTg                                                                                                            >  2:446000/1‑149 (MQ=255)
                                       gTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTg                                                                                                            >  2:513288/1‑149 (MQ=255)
                                        ttAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTg                                                                                                            <  4:56956/148‑1 (MQ=255)
                                        ttAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGc                                                                                                           >  1:416758/1‑149 (MQ=255)
                                            ggTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGttt                                                                                                       <  2:1050450/149‑1 (MQ=255)
                                            ggTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGttt                                                                                                       >  1:896060/1‑149 (MQ=255)
                                              tGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTg                                                                                                      >  4:378384/1‑148 (MQ=255)
                                                ccTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCaa                                                                                                   <  1:972379/149‑1 (MQ=255)
                                                       tgCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACActctc                                                                                            <  2:771637/149‑1 (MQ=255)
                                                          gcCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTAGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTa                                                                                          <  3:412437/148‑1 (MQ=255)
                                                           ccAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTAcc                                                                                        >  1:569592/1‑149 (MQ=255)
                                                                 gTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGc                                                                                  >  1:431805/1‑149 (MQ=255)
                                                                   gCATATAAACGATAACATTGACCTGTAGACTTGATTGTCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTAGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGcc                                                                                 <  3:819935/148‑1 (MQ=255)
                                                                   gCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATTCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGcc                                                                                 <  3:690687/148‑1 (MQ=255)
                                                                   gCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTAGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGccc                                                                                >  2:625708/1‑149 (MQ=255)
                                                                    cATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTAGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCcg                                                                               >  1:367435/1‑149 (MQ=255)
                                                                     atatAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCcgc                                                                              >  1:625842/1‑149 (MQ=255)
                                                                         aaaCGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCAt                                                                          >  1:679707/1‑149 (MQ=255)
                                                                          aaCGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTAGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCAtt                                                                         >  2:679805/1‑149 (MQ=255)
                                                                           aCGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCAttt                                                                        >  1:72749/1‑149 (MQ=255)
                                                                           aCGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCAttt                                                                        <  2:428131/149‑1 (MQ=255)
                                                                           aCGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCAtt                                                                         <  3:488351/148‑1 (MQ=255)
                                                                           aCGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTAGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCAttt                                                                        >  1:898129/1‑149 (MQ=255)
                                                                            cGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCAtttt                                                                       <  1:313970/149‑1 (MQ=255)
                                                                             gATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTg                                                                      >  1:50166/1‑149 (MQ=255)
                                                                              aTAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCAt                                                                          <  4:165436/144‑1 (MQ=255)
                                                                              aTAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCAt                                                                          >  3:165436/1‑144 (MQ=255)
                                                                               tAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGa                                                                     >  3:491904/1‑148 (MQ=255)
                                                                                 aCATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGacac                                                                  >  1:667666/1‑149 (MQ=255)
                                                                                 aCATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGaca                                                                   >  3:25068/1‑148 (MQ=255)
                                                                                   aTTGACCTGTAGACTTGATTATCATCGACAAATTCGCCGCTAACCTCCGCAAATTGCTGGGGCTTGGTGGCGTTGCACTCGGTCCCGCCATCTTGCCGACCCCTGCGTTTCGAACCCTCTCTCCCCCACGCCCTCGCATTTTGACACt                                                                 <  4:162886/148‑1 (MQ=255)
                                                                                           gTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACt                                                                 >  4:569300/1‑140 (MQ=255)
                                                                                           gTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCaataat                                                          <  3:779822/147‑1 (MQ=255)
                                                                                           gTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCaataat                                                          >  4:779822/1‑147 (MQ=255)
                                                                                           gTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCt                                                        <  2:996449/149‑1 (MQ=255)
                                                                                             aGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCtt                                                       <  4:849037/148‑1 (MQ=255)
                                                                                                cTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATa                                                   >  2:77635/1‑149 (MQ=255)
                                                                                                  tGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATAc                                                  >  4:511712/1‑148 (MQ=255)
                                                                                                     ttATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGga                                              <  2:1077567/149‑1 (MQ=255)
                                                                                                     ttATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGga                                              <  2:572795/149‑1 (MQ=255)
                                                                                                     ttATCATGGACAAATTCGACGCTAATCCCCTCAAATTGCTGGCGCTTAGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCgg                                               >  4:648699/1‑148 (MQ=255)
                                                                                                      tATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGga                                              >  4:40307/1‑148 (MQ=255)
                                                                                                      tATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGga                                              >  3:593772/1‑148 (MQ=255)
                                                                                                       aTCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGgaga                                            <  1:390813/149‑1 (MQ=255)
                                                                                                       aTCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGgag                                             <  4:25068/148‑1 (MQ=255)
                                                                                                       aTCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGgag                                             <  3:931124/148‑1 (MQ=255)
                                                                                                        tCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGTagag                                           >  2:96296/1‑149 (MQ=255)
                                                                                                          aTGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGtc                                         <  1:997345/149‑1 (MQ=255)
                                                                                                          aTGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGtc                                         <  1:175216/149‑1 (MQ=255)
                                                                                                            ggACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGtca                                        >  4:1039214/1‑148 (MQ=255)
                                                                                                             gACAAATTCGACGCTAATCGCCTCAAATTGCTGGCGCTTAGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGtcaa                                       <  3:648699/148‑1 (MQ=255)
                                                                                                              aCAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGgag                                             >  4:814586/1‑141 (MQ=255)
                                                                                                              aCAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGgag                                             <  3:814586/141‑1 (MQ=255)
                                                                                                               cAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGtcaatc                                     >  3:934078/1‑148 (MQ=255)
                                                                                                               cAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTAGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGtcaatc                                     >  3:417571/1‑148 (MQ=255)
                                                                                                                aaaTTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTCTGACACTCAATAATCTTCATACCGGAGAGtcaatcaa                                   >  2:865389/1‑149 (MQ=255)
                                                                                                                 aaTTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGtcaatcaa                                   <  3:378384/148‑1 (MQ=255)
                                                                                                                  aTTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAg                                 <  2:667666/149‑1 (MQ=255)
                                                                                                                   ttCGACGCTAATCGCCGCAAATTGCTGGCGCTTAGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAg                                 <  4:417571/148‑1 (MQ=255)
                                                                                                                     cGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCgg                              >  1:516913/1‑149 (MQ=255)
                                                                                                                        cGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAg                            >  4:869682/1‑148 (MQ=255)
                                                                                                                            aaTCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAg                                 >  3:485165/1‑139 (MQ=255)
                                                                                                                            aaTCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAg                                 <  4:485165/139‑1 (MQ=255)
                                                                                                                            aaTCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCCGAGAGTCAATCAAAGCGGAGttttt                       <  2:143140/149‑1 (MQ=255)
                                                                                                                             gcgccagcAATTTGCGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTCg                     >  1:485364/7‑149 (MQ=255)
                                                                                                                             aTCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGttttt                       <  4:697828/148‑1 (MQ=255)
                                                                                                                             aTCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTc                      <  1:954543/149‑1 (MQ=255)
                                                                                                                             aTCGCCGCAAATTGCTGGCGCTTAGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTc                      <  1:679805/149‑1 (MQ=255)
                                                                                                                               cgccgcAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTCg                     <  4:934078/148‑1 (MQ=255)
                                                                                                                                gccgcAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTCGa                    <  4:95642/148‑1 (MQ=255)
                                                                                                                                 ccgcAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTCGATg                  <  2:416758/149‑1 (MQ=255)
                                                                                                                                  cgcAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGtca                                        <  3:967664/126‑1 (MQ=255)
                                                                                                                                  cgcAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTCGATg                  <  3:925304/148‑1 (MQ=255)
                                                                                                                                  cgcAAATTGCTGGCGCTTAGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTCGATgg                 <  2:236144/149‑1 (MQ=255)
                                                                                                                                      aaTTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTCGATGGCaga             <  2:434894/149‑1 (MQ=255)
                                                                                                                                                gcTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTCGAt                   <  2:941051/133‑1 (MQ=255)
                                                                                                                                                gcTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTCGAt                   >  1:941051/1‑133 (MQ=255)
                                                                                                                                                gcTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTCGATGGCAGAGGCTATATTc   <  1:225824/149‑1 (MQ=255)
                                                                                                                                                 cTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTCGATGGCAGAGGCTATATTc   >  3:609027/1‑148 (MQ=255)
                                                                                                                                                  ttGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTCGATGGCAGAGGCTATATTCa  <  3:869682/148‑1 (MQ=255)
                                                                                                                                                   tggtggCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTa                                                                                          >  4:396797/1‑59 (MQ=255)
                                                                                                                                                   tggtggCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTa                                                                                          <  3:396797/59‑1 (MQ=255)
                                                                                                                                                   tggtggCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGtttt                        >  4:34534/1‑125 (MQ=255)
                                                                                                                                                   tggtggCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGtttt                        <  3:34534/125‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TGCTGGGGTTGAGTCATCTTGACGTCTGCTTTACGGGCGGTTAAGGTGCCTCTTGTGCGCCAGAAGTGCATATAAACGATAACATTGACCTGTAGACTTGATTATCATGGACAAATTCGACGCTAATCGCCGCAAATTGCTGGCGCTTGGTGGCGTTGCACTCGGTGCCGCCATCCTGCCGACCCCTGCGTTTGCAACACTCTCTACCCCACGCCCGCGCATTTTGACACTCAATAATCTTCATACCGGAGAGTCAATCAAAGCGGAGTTTTTCGATGGCAGAGGCTATATTCA  >  NC_000913/982969‑983262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: