Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,233,154 T→A 5.2% intergenic (‑124/+22) dsbB ← / ← nhaB oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,233,1540TA 5.2% 476.2 / 12.4 136intergenic (‑124/+22)dsbB/nhaBoxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (68/60);  new base A (3/4);  total (71/65)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 138 total aligned reads displayed.

AACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGC  >  NC_000913/1233006‑1233300
                                                                                                                                                    |                                                                                                                                                  
aaCATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCAtt                                                                                                                                                    >  1:128869/1‑149 (MQ=255)
aaCATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCAtt                                                                                                                                                    <  1:760796/149‑1 (MQ=255)
   aTTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGTAAACCGGGCATTtat                                                                                                                                                 >  1:112450/1‑149 (MQ=255)
    ttGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTtata                                                                                                                                                <  1:6410/149‑1 (MQ=255)
    ttGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTtat                                                                                                                                                 >  4:777660/1‑148 (MQ=255)
       gTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATg                                                                                                                                              <  4:180272/148‑1 (MQ=255)
        ttCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGc                                                                                                                                             >  4:533669/1‑148 (MQ=255)
         tccaaaaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGcc                                                                                                                                            <  3:932741/145‑1 (MQ=255)
         tCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGccc                                                                                                                                           <  2:494537/149‑1 (MQ=255)
         tCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGccc                                                                                                                                           <  2:819321/149‑1 (MQ=255)
         tCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGcc                                                                                                                                            >  4:467868/1‑148 (MQ=255)
           aaaaaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCgg                                                                                                                                         >  1:628006/1‑149 (MQ=255)
            aaaaTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCgg                                                                                                                                         >  4:837843/1‑148 (MQ=255)
                tCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAg                                                                                                                                     <  4:747912/148‑1 (MQ=255)
                    aaCATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttgtt                                                                                                                                <  1:88341/149‑1 (MQ=255)
                    aaCATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCAAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttgtt                                                                                                                                <  2:328584/149‑1 (MQ=255)
                      cATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTa                                                                                                                               >  4:390722/1‑148 (MQ=255)
                       aTAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATc                                                                                                                             >  1:888591/1‑149 (MQ=255)
                            cATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAg                                                                                                                         <  4:430881/148‑1 (MQ=255)
                            cATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAg                                                                                                                         >  4:296565/1‑148 (MQ=255)
                            cATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGc                                                                                                                        <  1:568289/149‑1 (MQ=255)
                             aTTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttgtt                                                                                                                                >  2:1071693/1‑140 (MQ=255)
                                tCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGtt                                                                                                                     >  4:402470/1‑148 (MQ=255)
                                 cccTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTg                                                                                                                    <  3:130383/148‑1 (MQ=255)
                                   cTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCt                                                                                                                  >  3:913964/1‑148 (MQ=255)
                                   cTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTa                                                                                                                 >  2:983514/1‑149 (MQ=255)
                                     gCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTAt                                                                                                                <  3:296565/148‑1 (MQ=255)
                                      cAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATcc                                                                                                              <  1:535203/149‑1 (MQ=255)
                                       aaTATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATcc                                                                                                              <  4:913964/148‑1 (MQ=255)
                                           tGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGcc                                                                                                          >  3:861575/1‑148 (MQ=255)
                                             cATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttgtt                                                                                                                                >  3:822973/1‑124 (MQ=255)
                                              aTAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGTAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGttt                                                                                                                                      >  2:318901/1‑115 (MQ=255)
                                              aTAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCAt                                                                                                       >  3:822145/1‑148 (MQ=255)
                                              aTAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGttt                                                                                                                                      <  1:318901/117‑3 (MQ=255)
                                               tAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCAttt                                                                                                     <  1:663864/149‑1 (MQ=255)
                                               tAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCAtt                                                                                                      >  3:189928/1‑148 (MQ=255)
                                               tAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATGTATATGCACGGTAAGTTGTTATCAAAGCTTTGCTATCCAGCCCAttt                                                                                                     <  2:832606/149‑1 (MQ=255)
                                                 gagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGc                                                                                                   >  2:875677/1‑149 (MQ=255)
                                                 gagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGc                                                                                                   >  1:742888/1‑149 (MQ=255)
                                                     gcgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaa                                                                                               <  2:628006/149‑1 (MQ=255)
                                                      cgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa                                                                                              <  1:103378/149‑1 (MQ=255)
                                                      cgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa                                                                                              <  1:866481/149‑1 (MQ=255)
                                                        cAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAAc                                                                                             >  4:78648/1‑148 (MQ=255)
                                                           tttAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCAtt                                                                                         >  1:802816/1‑149 (MQ=255)
                                                            ttAAACCAATTCATTCGCTGCGCCACCAGGAGGCATCAAAAATCGGACGCAATTGGATAATTCTCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAATCGTTGCTATCCAGCCCATTTGCATAAACCAtt                                                                                         >  3:890484/1‑148 (MQ=255)
                                                            ttAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCAtt                                                                                         >  3:631896/1‑148 (MQ=255)
                                                               aaCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGt                                                                                     <  2:973263/149‑1 (MQ=255)
                                                                aCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGtt                                                                                    <  2:645888/149‑1 (MQ=255)
                                                                aCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGtt                                                                                    >  1:1035562/1‑149 (MQ=255)
                                                                  cAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGc                                                                                                                        >  1:196392/1‑111 (MQ=255)
                                                                  cAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACACAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGt                                                                                                                      <  4:556743/113‑1 (MQ=255)
                                                                  cAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACACAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGt                                                                                                                      >  3:556743/1‑113 (MQ=255)
                                                                   aaTTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTAc                                                                                  >  4:226477/1‑148 (MQ=255)
                                                                   aaTTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTAc                                                                                  <  3:402470/148‑1 (MQ=255)
                                                                     ttcattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAg                                                                                >  3:465909/1‑148 (MQ=255)
                                                                      tcattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAggg                                                                              <  1:586783/149‑1 (MQ=255)
                                                                      tcattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAgg                                                                               <  4:869124/148‑1 (MQ=255)
                                                                        attcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGc                                                                            >  1:78376/1‑149 (MQ=255)
                                                                         ttcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCa                                                                           >  2:376474/1‑149 (MQ=255)
                                                                          tcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCaa                                                                          <  2:943892/149‑1 (MQ=255)
                                                                            gCTGCGCCACCAGTAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGc                                                                        <  2:783377/149‑1 (MQ=255)
                                                                                    accaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACt                                                                 >  4:474469/1‑148 (MQ=255)
                                                                                     ccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTc                                                                >  3:112099/1‑148 (MQ=255)
                                                                                      caGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCg                                                               <  3:6974/148‑1 (MQ=255)
                                                                                      caGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGa                                                              <  2:19607/149‑1 (MQ=255)
                                                                                         gaggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGc                                                           >  2:713441/1‑149 (MQ=255)
                                                                                          aggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGc                                                           <  3:952533/148‑1 (MQ=255)
                                                                                               gcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCAt                                                                                                 <  2:1001673/105‑1 (MQ=255)
                                                                                               gcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCAt                                                                                                 >  1:1001673/1‑105 (MQ=255)
                                                                                                caAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAg                                                                         <  4:427517/128‑1 (MQ=255)
                                                                                                caAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAg                                                                         >  3:427517/1‑128 (MQ=255)
                                                                                                caAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCACTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACtcgtcggcttgct                                                     >  4:277575/1‑141 (MQ=255)
                                                                                                 aaaaaTAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCaga                                                        <  2:882879/144‑1 (MQ=255)
                                                                                                  aaaaTAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGccc                                                                                                         <  4:985265/94‑1 (MQ=255)
                                                                                                  aaaaTAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGccc                                                                                                         >  3:985265/1‑94 (MQ=255)
                                                                                                  aaaaTAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAg                                                   >  3:815790/1‑148 (MQ=255)
                                                                                                          aCGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAgt                                          >  2:243764/1‑149 (MQ=255)
                                                                                                           cGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGACAAGCGTAAACTCGACGCAGAGCAAGCCGACGAgt                                          >  4:690996/1‑148 (MQ=255)
                                                                                                             cAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAgtgt                                        >  3:296778/1‑148 (MQ=255)
                                                                                                               aTTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCag                                                         >  3:671698/1‑129 (MQ=255)
                                                                                                               aTTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCag                                                         <  4:671698/129‑1 (MQ=255)
                                                                                                                ttGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAgg                                    <  1:713441/149‑1 (MQ=255)
                                                                                                                    aCAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAg                                           >  4:46276/1‑138 (MQ=255)
                                                                                                                     cAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAg                               <  1:618334/149‑1 (MQ=255)
                                                                                                                      aaTTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAgg                              >  1:778991/1‑149 (MQ=255)
                                                                                                                      aaTTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAgg                              <  2:718076/149‑1 (MQ=255)
                                                                                                                       aTTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAgg                              >  4:82957/1‑148 (MQ=255)
                                                                                                                        ttATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAggg                             <  3:582120/148‑1 (MQ=255)
                                                                                                                            cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTaa                        <  1:376474/149‑1 (MQ=255)
                                                                                                                            cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTaa                        <  2:238128/149‑1 (MQ=255)
                                                                                                                                  aaaaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgacg                                             <  3:317694/122‑1 (MQ=255)
                                                                                                                                  aaaaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgacg                                             >  4:317694/1‑122 (MQ=255)
                                                                                                                                  aaaaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAg                   >  3:488614/1‑148 (MQ=255)
                                                                                                                                    aaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAg                                                   >  4:884799/1‑114 (MQ=255)
                                                                                                                                    aaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAg                                                   <  3:884799/114‑1 (MQ=255)
                                                                                                                                       acttaCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCag                                                         >  4:470164/5‑105 (MQ=255)
                                                                                                                                       acttaCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGAGGCAAGCGTAAACTCGACGCag                                                         <  3:470164/101‑1 (MQ=255)
                                                                                                                                          taCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATcc           >  3:850459/2‑148 (MQ=255)
                                                                                                                                             cGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCcaca        >  4:600947/1‑148 (MQ=255)
                                                                                                                                              gggCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCacac       >  4:949809/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGC  >  NC_000913/1233006‑1233300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: