Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,703,251 A→T 5.6% intergenic (+17/+17) add → / ← ydgJ adenosine deaminase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,703,2510AT 5.6% 307.1 / 7.4 89intergenic (+17/+17)add/ydgJadenosine deaminase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (42/42);  new base T (2/3);  total (44/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

ATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGC  >  NC_000913/1703104‑1703396
                                                                                                                                                   |                                                                                                                                                 
ataCCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCaaa                                                                                                                                                  <  1:18583/149‑1 (MQ=255)
       tGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCAcc                                                                                                                                            <  4:886165/148‑1 (MQ=255)
         ccGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCAccatc                                                                                                                                         <  2:754295/149‑1 (MQ=255)
           gcgcCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCAccatcca                                                                                                                                       <  1:652161/149‑1 (MQ=255)
                aGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCAtttt                                                                                                                                   >  3:990699/1‑148 (MQ=255)
                   tgctgGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAACTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCAt                                                                                                                               <  2:921758/149‑1 (MQ=255)
                    gctgGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCa                                                                                                                                <  4:33251/147‑1 (MQ=255)
                    gctgGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCa                                                                                                                                >  3:33251/1‑147 (MQ=255)
                       gggTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCaa                                                                                                                           <  1:301757/149‑1 (MQ=255)
                       gggTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCa                                                                                                                            >  4:594312/1‑148 (MQ=255)
                                  cgAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTc                                                                                                                <  2:921405/149‑1 (MQ=255)
                                  cgAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTc                                                                                                                <  2:203105/149‑1 (MQ=255)
                                      cAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCACCATCCAttt                                                                                                                                    >  4:724738/1‑125 (MQ=255)
                                      cAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCATTTGCACCATCCAttt                                                                                                                                    <  3:724738/125‑1 (MQ=255)
                                      cAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTcgcg                                                                                                             >  3:119018/1‑148 (MQ=255)
                                           ccGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATg                                                                                                        >  4:156960/1‑148 (MQ=255)
                                           ccGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATg                                                                                                        >  3:674848/1‑148 (MQ=255)
                                             gCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTATCAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGtt                                                                                                      <  4:674848/148‑1 (MQ=255)
                                               cAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTg                                                                                                    <  3:638430/148‑1 (MQ=255)
                                                aGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTgg                                                                                                   <  4:119018/148‑1 (MQ=255)
                                                aGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGc                                                                                                  <  1:544248/149‑1 (MQ=255)
                                                  gCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCgg                                                                                                >  2:990699/1‑149 (MQ=255)
                                                   cacaGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGa                                                                                               >  1:121986/1‑149 (MQ=255)
                                                       gATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTgg                                                                                                   >  1:100741/1‑141 (MQ=255)
                                                       gATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTgg                                                                                                   <  2:100741/141‑1 (MQ=255)
                                                        aTTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGa                                                                                          <  2:146686/149‑1 (MQ=255)
                                                         ttAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGa                                                                                          <  3:928892/148‑1 (MQ=255)
                                                         ttAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGa                                                                                          <  4:208491/148‑1 (MQ=255)
                                                          tAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCTATGTTTGGCGGATTCGATg                                                                                        <  2:147213/149‑1 (MQ=255)
                                                                tCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAg                                                                                   >  4:160205/1‑148 (MQ=255)
                                                                tCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGc                                                                                  <  2:817298/149‑1 (MQ=255)
                                                                 cTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCt                                                                                 >  2:984169/1‑149 (MQ=255)
                                                                   ggAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCtca                                                                               >  2:14721/1‑149 (MQ=255)
                                                                   ggAAATGGCTGTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCtc                                                                                >  4:615194/1‑148 (MQ=255)
                                                                    gAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCtca                                                                               <  3:156960/148‑1 (MQ=255)
                                                                     aaaTGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCtcaat                                                                             >  1:477781/1‑149 (MQ=255)
                                                                       aTGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCtcaatca                                                                           >  2:864267/1‑149 (MQ=255)
                                                                         gtttttCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAAc                                                                         >  2:659100/4‑149 (MQ=255)
                                                                          tttttCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAAc                                                                         >  4:390463/3‑148 (MQ=255)
                                                                          tttttCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAAc                                                                         >  3:924750/3‑148 (MQ=255)
                                                                          gCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAAc                                                                         >  4:669200/1‑148 (MQ=255)
                                                                            tttCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTc                                                                       <  3:187137/148‑1 (MQ=255)
                                                                               ccTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCAt                                                                    <  4:32607/148‑1 (MQ=255)
                                                                                cTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTa                                                                  >  1:495288/1‑149 (MQ=255)
                                                                                cTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTa                                                                  >  1:585617/1‑149 (MQ=255)
                                                                                        tGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGAtt                                                          <  2:706330/149‑1 (MQ=255)
                                                                                         gAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGAt                                                                                         >  4:978872/1‑117 (MQ=255)
                                                                                         gAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGAt                                                                                         <  3:978872/117‑1 (MQ=255)
                                                                                         gAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGAtt                                                          >  3:478252/1‑148 (MQ=255)
                                                                                         gAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGAtt                                                          >  4:1046729/1‑148 (MQ=255)
                                                                                          aGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTg                                                         <  3:708602/148‑1 (MQ=255)
                                                                                            gAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGcc                                                       <  4:605985/148‑1 (MQ=255)
                                                                                             aaaaaCGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGt                                                                                                       <  4:234179/99‑1 (MQ=255)
                                                                                             aaaaaCGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGt                                                                                                       >  3:234179/1‑99 (MQ=255)
                                                                                             aaaaaCGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCt                                                      >  3:72659/1‑148 (MQ=255)
                                                                                             aaaaaCGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCt                                                      >  4:319943/1‑148 (MQ=255)
                                                                                             aaaaaCGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTg                                                     <  2:477781/149‑1 (MQ=255)
                                                                                                      cACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCgg                                            <  2:585617/149‑1 (MQ=255)
                                                                                                       aCTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGa                                           >  1:31974/1‑149 (MQ=255)
                                                                                                        cTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGaa                                          <  2:679562/149‑1 (MQ=255)
                                                                                                            gagaAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCg                                       <  3:1046729/148‑1 (MQ=255)
                                                                                                              gaAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGa                                     >  3:10254/1‑148 (MQ=255)
                                                                                                              gaAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGa                                     >  4:1024583/1‑148 (MQ=255)
                                                                                                              gaAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGa                                     >  4:849176/1‑148 (MQ=255)
                                                                                                              gaAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGAt                                    >  1:750325/1‑149 (MQ=255)
                                                                                                               aaaaaGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGAt                                    <  4:993769/148‑1 (MQ=255)
                                                                                                                 aaaGTCGCCGCGCAGTAACAAAATGGATGGTGCAAATGGACCAGCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGAttt                                  >  4:89517/1‑148 (MQ=255)
                                                                                                                  aaGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGAtttt                                 <  3:1024583/148‑1 (MQ=255)
                                                                                                                            gAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCa                      <  2:31974/149‑1 (MQ=255)
                                                                                                                             aaGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCa                      <  4:72659/148‑1 (MQ=255)
                                                                                                                                tAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGc                                               >  3:22491/1‑120 (MQ=255)
                                                                                                                                tAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGc                                               <  4:22491/120‑1 (MQ=255)
                                                                                                                                  gaaaaaTGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTaa                 >  3:918952/3‑148 (MQ=255)
                                                                                                                                  aCAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCtcaat                                                                             <  2:246441/88‑1 (MQ=255)
                                                                                                                                  aCAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCtcaat                                                                             >  1:246441/1‑88 (MQ=255)
                                                                                                                                  aCAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAAc                >  2:748157/1‑149 (MQ=255)
                                                                                                                                   cAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAAc                >  3:853345/1‑148 (MQ=255)
                                                                                                                                    aaaaTGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGc              >  1:587241/1‑149 (MQ=255)
                                                                                                                                       atggatggTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATc           <  1:14721/149‑1 (MQ=255)
                                                                                                                                         ggatggTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCa          >  4:584082/1‑148 (MQ=255)
                                                                                                                                         ggatggTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCa          <  3:849176/148‑1 (MQ=255)
                                                                                                                                           atggTGCATTTGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGAt                                                                                              >  4:192317/1‑62 (MQ=37)
                                                                                                                                           atggTGCATTTGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGAt                                                                                              <  3:192317/62‑1 (MQ=37)
                                                                                                                                           atggTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTGTCTATCGCCATTTAACGCATCACg        >  4:817109/1‑148 (MQ=255)
                                                                                                                                            tggTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGaa      <  1:748157/149‑1 (MQ=255)
                                                                                                                                              gTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAAt     <  3:390463/148‑1 (MQ=255)
                                                                                                                                              gTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAAt     <  4:918952/148‑1 (MQ=255)
                                                                                                                                               tGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGaa      >  3:1018117/1‑146 (MQ=255)
                                                                                                                                               tGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGaa      <  4:1018117/146‑1 (MQ=255)
                                                                                                                                                 cAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGc  <  4:924750/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTGAGGAAAAACGCGCACTGCGAGAAAAAGTCGCCGCGAAGTAACAAAATGGATGGTGCAAATGCACCATCCATTTTTCATGCAAGGCACAAAGTCGCGCGATGTTTGGCGGATTCGATGCCCAGCTCAATCAACTCCATTACCTGGATTGCCTGGCTTGCCGGAACCGGATTTTCACCATCGCCATTTAACGCATCACGAATAGC  >  NC_000913/1703104‑1703396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: