Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,795,228 G→C 7.5% intergenic (‑76/+25) btuC ← / ← ihfA vitamin B12 ABC transporter permease/integration host factor (IHF), DNA‑binding protein, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,795,2280GC 7.5% 221.3 / 8.0 67intergenic (‑76/+25)btuC/ihfAvitamin B12 ABC transporter permease/integration host factor (IHF), DNA‑binding protein, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (31/31);  new base C (3/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

TCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGT  >  NC_000913/1795086‑1795367
                                                                                                                                              |                                                                                                                                           
tCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGc                                                                                                                                       >  1:533100/1‑149 (MQ=255)
 cAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGc                                                                                                                                       >  4:58353/1‑148 (MQ=255)
    aaCTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCttt                                                                                                                                   <  2:560335/149‑1 (MQ=255)
    aaCTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCttt                                                                                                                                   >  2:651090/1‑149 (MQ=255)
     aCTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCTCTGCGGCCtttt                                                                                                                                  <  2:535409/149‑1 (MQ=255)
          cAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTttagtt                                                                                                                             <  1:651090/149‑1 (MQ=255)
          cAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTttagtt                                                                                                                             <  2:787068/149‑1 (MQ=255)
           aGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTttagtta                                                                                                                            >  2:350857/1‑149 (MQ=255)
              cATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGAt                                                                                                                         <  2:533100/149‑1 (MQ=255)
              cATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGAt                                                                                                                         <  2:787637/149‑1 (MQ=255)
                  ataaCCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGa                                                                                                                     <  2:272681/149‑1 (MQ=255)
                  ataaCCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGa                                                                                                                     <  1:801164/149‑1 (MQ=255)
                        aGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACt                                                                                                                >  3:513534/1‑148 (MQ=255)
                           gAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCtttt                                                                                                                                  >  1:337409/1‑127 (MQ=255)
                           gAATATGTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCtttt                                                                                                                                  <  2:337409/127‑1 (MQ=255)
                             atatTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCt                                                                                                           >  3:811820/1‑148 (MQ=255)
                              tatTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCtt                                                                                                          <  4:618107/148‑1 (MQ=255)
                               atTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCttt                                                                                                         <  3:93017/148‑1 (MQ=255)
                                ttttGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTg                                                                                                        <  3:58353/148‑1 (MQ=255)
                                 tttGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTgg                                                                                                       >  3:928702/1‑148 (MQ=255)
                                  ttGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGc                                                                                                     <  1:512574/149‑1 (MQ=255)
                                     tCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGa                                                                                                   >  4:420396/1‑148 (MQ=255)
                                     tCGCGGATGTTGGCGGGCAAGTGTCAGCATGGTATGCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGaa                                                                                                  >  2:1046857/1‑149 (MQ=255)
                                                  cGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGAccc                                                                                     <  1:863229/149‑1 (MQ=255)
                                                     gCAAGTGTCAGCATGGTATCCGTTCTGCTTAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTGGTCTTTGGGCGAAGCGTTTTCTACCCGGc                                                                                  >  2:420804/1‑149 (MQ=255)
                                                        aGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCtt                                                                                <  4:167122/148‑1 (MQ=255)
                                                        aGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCtt                                                                                <  4:928702/148‑1 (MQ=255)
                                                           gtCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTa                                                                            >  1:930614/1‑149 (MQ=255)
                                                            tCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTaa                                                                           <  2:412149/149‑1 (MQ=255)
                                                             cAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTaa                                                                           <  4:811820/148‑1 (MQ=255)
                                                             cAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAAc                                                                          <  2:65304/149‑1 (MQ=255)
                                                                         cGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGt                                                              <  2:196478/149‑1 (MQ=255)
                                                                          gTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCttt                                                                                                                                   >  2:381371/1‑79 (MQ=255)
                                                                          gTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCttt                                                                                                                                   <  1:381371/79‑1 (MQ=255)
                                                                            tCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCAGCCCGGGTCTg                                                           <  1:560679/149‑1 (MQ=255)
                                                                              tgctgAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGaa                                                         <  1:420804/149‑1 (MQ=255)
                                                                               gctgAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGaa                                                         <  3:840355/148‑1 (MQ=255)
                                                                                ctgAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAg                                                        >  4:313046/1‑148 (MQ=255)
                                                                                 tgAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTg                                                                                                        <  4:308340/99‑1 (MQ=255)
                                                                                 tgAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTg                                                                                                        >  3:308340/1‑99 (MQ=255)
                                                                                      tgtCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGa                                                          <  3:906103/140‑1 (MQ=255)
                                                                                      tgtCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGa                                                          >  4:906103/1‑140 (MQ=255)
                                                                                       gtCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTcacca                                                 <  4:513534/148‑1 (MQ=255)
                                                                                        tCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTca                                                    >  2:802876/1‑144 (MQ=255)
                                                                                        tCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTca                                                    <  1:802876/144‑1 (MQ=255)
                                                                                        tCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCAcg                                               >  1:774498/1‑149 (MQ=255)
                                                                                              cGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAATAAACGCCGCAGAGCGGCTTTGTTAGTTAGTTCAGATTACTCGTCTTTGGACGAGGCGTTTACGACCCGTCTGTTTAACTTCTGCCCGGGTCTGAACGTCACCACGCCCCg                                          <  4:987443/148‑1 (MQ=255)
                                                                                                ttGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGc                                       >  2:476832/1‑149 (MQ=255)
                                                                                                    ttttACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGt                                    >  3:11407/1‑148 (MQ=255)
                                                                                                           gTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGaa                            >  2:216294/1‑149 (MQ=255)
                                                                                                            tGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGaa                            >  4:903239/1‑148 (MQ=255)
                                                                                                                tctTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATcc                       >  1:147529/1‑149 (MQ=255)
                                                                                                                tctTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATcc                       <  1:350857/149‑1 (MQ=255)
                                                                                                                tctTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATcc                       >  1:403092/1‑149 (MQ=255)
                                                                                                                 ctTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATcc                       >  4:333347/1‑148 (MQ=255)
                                                                                                                          tGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGtt              >  3:323796/1‑148 (MQ=255)
                                                                                                                              aagaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCTTCGCCCGTTTTCgg         >  1:322807/1‑149 (MQ=255)
                                                                                                                               agaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCgg         >  4:743032/1‑148 (MQ=255)
                                                                                                                               agaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCgg         <  4:323796/148‑1 (MQ=255)
                                                                                                                                gaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGt       >  1:995408/1‑149 (MQ=255)
                                                                                                                                  aaaaaGGCCGCTCTGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTa                                                                            <  4:505985/78‑1 (MQ=38)
                                                                                                                                  aaaaaGGCCGCTCTGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTa                                                                            >  3:505985/1‑78 (MQ=38)
                                                                                                                                  aaaaaGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATCACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGtt      <  4:84103/148‑1 (MQ=255)
                                                                                                                                   aaaaGGCCGCTCTGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTAc    >  1:42862/1‑149 (MQ=255)
                                                                                                                                    aaaGGGCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGCCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACAACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTCTTCGGGTTACg   >  1:862676/1‑149 (MQ=255)
                                                                                                                                     aaGGCCGCTCTGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTGTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTCATGGGAATATCCTCACCCGTGTTCGGGTTACGt  >  2:404842/1‑149 (MQ=255)
                                                                                                                                     aaGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACg   >  4:319348/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                           
TCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGT  >  NC_000913/1795086‑1795367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: