Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,864,768	T→C	11.1%	intergenic (+34/+14)	yeaD → / ← yeaE	D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,864,768	0	T	C	11.1%	148.4 / 9.8	54	intergenic (+34/+14)	yeaD/yeaE	D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (24/24);  new base C (3/3);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

GGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTG  >  NC_000913/1864624‑1864915                                                                                                                                                    |                                                                                                                                                    ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCg                                                                                                                                                  >  3:645519/1‑148 (MQ=255)    tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGAT‑GAATTGCACCCGGcac                                                                                                                                              <  1:807999/149‑1 (MQ=255)      cAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGAT‑GCATTGCACCCGGcaca                                                                                                                                             <  4:504157/148‑1 (MQ=255)        aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGcacaca                                                                                                                                           >  4:546864/1‑148 (MQ=255)            aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATc                                                                                                                                      >  1:1059023/1‑149 (MQ=255)             tttgtttgTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAG‑GAAAACTGCACCACCTGCCCAAATCATTCCGCG‑TGCCAAACGGTAATTTTACG‑TAATGTTTGGTTCCCGGTGGCATTCCTTCCGGCCCACAAAATc                                                                                                                                      >  4:252860/1‑148 (MQ=255)               tgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATcac                                                                                                                                    <  4:184948/148‑1 (MQ=255)                          aaaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAgc                                                                                                                        >  2:106898/1‑149 (MQ=255)                                  tACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGttt                                                                                                                 >  3:822431/1‑148 (MQ=255)                                    cGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGttttt                                                                                                               <  3:262058/148‑1 (MQ=255)                                        tCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                           >  3:850307/1‑148 (MQ=255)                                         cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCttt                                                                                                          <  4:645519/148‑1 (MQ=255)                                           aaaaccgaaaaaGTGGCCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                        <  3:252860/141‑1 (MQ=255)                                                gCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTTGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                                                                                  >  1:719731/1‑149 (MQ=255)                                                      aGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                                <  1:95288/129‑1 (MQ=255)                                                      aGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                                >  2:95288/1‑129 (MQ=255)                                                             aaaGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGAT‑GCATTGCACCCGGcaca                                                                                                                                             <  3:264126/93‑1 (MQ=38)                                                             aaaGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGAT‑GCATTGCACCCGGcaca                                                                                                                                             >  4:264126/1‑93 (MQ=37)                                                              aaGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                                                                                     >  3:375451/1‑148 (MQ=255)                                                                 agaAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAg                                                                                  <  4:375451/148‑1 (MQ=255)                                                                    aaCCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCAt                                                                               <  3:546864/148‑1 (MQ=255)                                                                        tGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTa                                                                          >  1:507666/1‑149 (MQ=255)                                                                                   gcgcAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGatat                                                                                             >  1:1070392/1‑120 (MQ=255)                                                                                   gcgcAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGatat                                                                                             <  2:1070392/120‑1 (MQ=255)                                                                                    cgcAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                               <  1:106898/149‑1 (MQ=255)                                                                                        aTCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaagaa                                                            >  4:92921/1‑148 (MQ=255)                                                                                        aTCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaagaa                                                            >  3:1030054/1‑148 (MQ=255)                                                                                            aTTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGtt                                                        <  3:235836/148‑1 (MQ=255)                                                                                            aTTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGtt                                                        <  4:746849/148‑1 (MQ=255)                                                                                                   ttGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCa                                                >  2:975348/1‑149 (MQ=255)                                                                                                      cGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGGAGAAAGTTCGACCTCAAg                                              <  4:963650/148‑1 (MQ=255)                                                                                                          aCGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcaca                                          <  3:520562/148‑1 (MQ=255)                                                                                                            gTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGcc                                       <  2:1059023/149‑1 (MQ=255)                                                                                                             ttAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGcc                                       <  4:822431/148‑1 (MQ=255)                                                                                                                  tttACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGaaa                                                           <  1:149382/124‑1 (MQ=255)                                                                                                                  tttACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGaaa                                                           >  2:149382/1‑124 (MQ=255)                                                                                                                  tttACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAtt                                  >  2:735327/1‑149 (MQ=255)                                                                                                                       gTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                             >  2:892793/1‑149 (MQ=255)                                                                                                                       gTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTGATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGTCGCATTttgtt                             <  1:877222/149‑1 (MQ=255)                                                                                                                        ttAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                                     >  3:816884/1‑124 (MQ=255)                                                                                                                        ttAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                                     <  4:816884/124‑1 (MQ=255)                                                                                                                        ttAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgttg                            <  1:695077/149‑1 (MQ=255)                                                                                                                         tAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgttg                            <  4:925582/148‑1 (MQ=255)                                                                                                                             gttgtGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACAt                       >  2:135448/1‑149 (MQ=255)                                                                                                                               tgtgtgCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg                     <  1:735327/149‑1 (MQ=255)                                                                                                                                      cggat‑GCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc               >  4:6206/5‑148 (MQ=255)                                                                                                                                          t‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTgg           <  4:113683/148‑1 (MQ=255)                                                                                                                                             cattGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGcc         >  3:1009369/4‑148 (MQ=255)                                                                                                                                              aaTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGc       >  2:466117/1‑149 (MQ=255)                                                                                                                                              aaTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGc       >  1:1016385/1‑149 (MQ=255)                                                                                                                                                  cATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCtttt   <  2:539458/149‑1 (MQ=255)                                                                                                                                                  cATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCttt    <  3:1042182/148‑1 (MQ=255)                                                                                                                                                   aTCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTg  >  2:169731/1‑149 (MQ=255)                                                                                                                                                   aTCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTg  >  2:840053/1‑149 (MQ=255)                                                                                                                                                    tCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTg  >  4:735784/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCA‑CATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAA‑TTTACGTTAATGTTGTGTGCCGGGT‑GCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTG  >  NC_000913/1864624‑1864915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑