| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,954,256 | T→A | 7.2% | I272N (ATT→AAT) | cmoB → | tRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,954,256 | 0 | T | A | 7.2% | 370.5 / 14.4 | 107 | I272N (ATT→AAT) | cmoB | tRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (50/49); new base A (4/4); total (54/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
GAAACGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCG > NC_000913/1954114‑1954401 | gAAACGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGa > 2:201122/1‑149 (MQ=255) aaaCGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGa > 4:325243/1‑148 (MQ=255) aaaCGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt < 1:201122/149‑1 (MQ=255) aaaCGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCAATGCGGAt < 1:78688/149‑1 (MQ=255) aaCGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAtg > 1:58596/1‑149 (MQ=255) aCGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAtgt < 2:782925/149‑1 (MQ=255) aCGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAtgt < 2:28581/149‑1 (MQ=255) gCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGa < 2:494538/149‑1 (MQ=255) gCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGa < 1:444587/149‑1 (MQ=255) cTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGa > 4:244655/1‑148 (MQ=255) cTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAg > 2:725554/1‑149 (MQ=255) tGGTTATTGATGGTGATGAGAACACTGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAg > 4:903708/1‑148 (MQ=255) tGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGc < 2:1007775/149‑1 (MQ=255) ggTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGc > 4:191228/1‑148 (MQ=255) ttATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGtt > 1:472611/1‑149 (MQ=255) ttATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGt < 3:133170/148‑1 (MQ=255) aTTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTac < 1:725554/149‑1 (MQ=255) gATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTaccac < 2:133802/149‑1 (MQ=255) ggTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTAccacca > 3:794726/1‑148 (MQ=255) tgatgaGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGCAGATATCCGCATTGCGGATGTGAGCGTTACCACcaca > 3:174581/1‑148 (MQ=255) atgaGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACagaa < 2:570522/149‑1 (MQ=255) tgaGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACagaa > 4:1011851/1‑148 (MQ=255) gaACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACcaca < 2:967085/142‑1 (MQ=255) gaACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACcaca > 1:967085/1‑142 (MQ=255) aaCACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGagca > 2:1053119/1‑149 (MQ=255) aaCACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGagca > 2:139660/1‑149 (MQ=255) acacGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGagcag < 1:761942/149‑1 (MQ=255) acacGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGagca > 4:1026240/1‑148 (MQ=255) gTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGAc > 3:799064/1‑148 (MQ=255) gTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGAc > 4:173332/1‑148 (MQ=255) tGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACg < 3:907493/148‑1 (MQ=255) tACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCCGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGaa > 2:860544/1‑149 (MQ=255) aGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCgg < 4:585323/104‑1 (MQ=255) aGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCgg > 3:585323/1‑104 (MQ=255) ggCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAatggat < 1:415563/149‑1 (MQ=255) cGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAatggatgg < 1:902203/149‑1 (MQ=255) gATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt > 2:180660/1‑149 (MQ=255) gTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTAcc < 2:58596/149‑1 (MQ=255) aCGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGaa < 2:573649/149‑1 (MQ=255) gcAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCAc > 1:1053641/1‑126 (MQ=255) gcAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCAc < 2:1053641/126‑1 (MQ=255) gcAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGc > 2:341599/1‑149 (MQ=255) cAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGc > 4:162441/1‑148 (MQ=255) aaaTGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTg < 2:784388/149‑1 (MQ=255) aaTGTGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTAGTTTTGTAGATATCCGCAATGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTg > 4:903302/1‑148 (MQ=255) gTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGAt < 1:341599/149‑1 (MQ=255) tGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGtt < 1:1035237/88‑1 (MQ=255) tGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTcc < 1:139660/149‑1 (MQ=255) tGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTc > 3:787481/1‑148 (MQ=255) gTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTcc > 4:5243/1‑148 (MQ=255) tCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGc < 2:1058450/73‑1 (MQ=255) tCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGc > 1:1058450/1‑73 (MQ=255) cTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTc > 4:188793/1‑148 (MQ=255) cTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTc > 4:448787/1‑148 (MQ=255) tATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGa > 2:54333/1‑149 (MQ=255) aTTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGa > 4:419876/1‑148 (MQ=255) ttCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGAcc > 3:482919/1‑148 (MQ=255) ttCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGAcc > 3:45170/1‑148 (MQ=255) tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGATGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATTGTTACCGAATCGCTGGCCGATTTCCTCGACCca < 2:632503/149‑1 (MQ=255) cATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCca < 4:787481/148‑1 (MQ=255) cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATg > 1:291852/1‑149 (MQ=255) cgcgCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCgg < 1:155568/149‑1 (MQ=255) cgcgCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCgg < 2:331447/149‑1 (MQ=255) cgcgCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCgg < 1:824741/149‑1 (MQ=255) cgcgCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCg < 4:799064/148‑1 (MQ=255) ggcgctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTaaa < 3:903708/148‑1 (MQ=255) tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTg > 2:906459/1‑149 (MQ=255) tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTg < 1:394353/149‑1 (MQ=255) tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTACGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGt < 3:903302/148‑1 (MQ=255) aaaaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGaa < 2:787554/149‑1 (MQ=255) aaaaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGa < 3:86061/148‑1 (MQ=255) aaaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAg < 1:439987/149‑1 (MQ=255) gCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTAt < 4:482919/148‑1 (MQ=255) gaagaaGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGGAAAACGGTGGAAGGTTATCCg > 3:95613/1‑148 (MQ=255) aagaagTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGc > 2:267435/1‑149 (MQ=255) aagaagTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGc > 2:1009211/1‑149 (MQ=255) gaagTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCAc < 2:947610/149‑1 (MQ=255) aagTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCAc > 4:515749/1‑148 (MQ=255) agTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCt < 1:906459/149‑1 (MQ=255) gTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgc > 2:424736/1‑149 (MQ=255) tttGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcg < 3:5243/148‑1 (MQ=255) ttGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcgc < 4:706811/148‑1 (MQ=255) tAGATATCCGCATTGCGGATGTGAGCGTAACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGt > 3:859856/1‑148 (MQ=255) tcacatCCGCAATGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTATCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGt > 1:400485/5‑117 (MQ=255) tcacatCCGCAATGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTATCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGt < 2:400485/113‑1 (MQ=255) aGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTg > 3:282450/1‑148 (MQ=255) aGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTg > 3:373125/1‑148 (MQ=255) gATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCt < 1:424736/149‑1 (MQ=255) acatCCGCAATGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGa < 1:943680/89‑1 (MQ=255) acatCCGCAATGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGc < 1:828897/145‑1 (MQ=255) acatCCGCAATGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGc > 2:828897/3‑147 (MQ=255) tatCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGa < 1:826571/149‑1 (MQ=255) tCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGAt < 3:419876/148‑1 (MQ=255) ccGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTg > 2:755741/1‑149 (MQ=255) cGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTgc > 2:512445/1‑149 (MQ=255) gCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGa > 3:74753/1‑94 (MQ=255) gCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGa < 4:74753/94‑1 (MQ=255) gCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTgcg > 1:873049/1‑149 (MQ=255) gCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTgcg > 2:512111/1‑149 (MQ=255) cATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTgcg > 3:372394/1‑148 (MQ=255) | GAAACGCTGGTTATTGATGGTGATGAGAACACGGTGCTGGTACCAGGCGATCGTTACGCGCAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCG > NC_000913/1954114‑1954401 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |