Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,009,733 C→T 7.7% intergenic (+21/‑88) yedF → / → yedK putative TusA family sulfurtransferase/DUF159 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,009,7330CT 7.7% 372.1 / 15.1 116intergenic (+21/‑88)yedF/yedKputative TusA family sulfurtransferase/DUF159 family protein
Reads supporting (aligned to +/- strand):  ref base C (58/49);  new base T (5/4);  total (63/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 121 total aligned reads displayed.

TCCTGGAAGTGGTGAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAG  >  NC_000913/2009585‑2009881
                                                                                                                                                    |                                                                                                                                                    
tCCTGGAAGTGGTGAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGAcc                                                                                                                                                      <  2:832149/149‑1 (MQ=255)
    ggAAGTGGTGAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCcaca                                                                                                                                                  <  1:606379/149‑1 (MQ=255)
    ggAAGTGGTGAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCcac                                                                                                                                                   <  3:991532/148‑1 (MQ=255)
       aGTGGTGAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGc                                                                                                                                                <  4:821941/148‑1 (MQ=255)
        gtggtgAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCg                                                                                                                                               <  3:785820/148‑1 (MQ=255)
        gtggtgAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGAGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGGGTCACAGCg                                                                                                                                               <  3:280060/148‑1 (MQ=255)
          ggtgAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGGGTCACAGCGGGa                                                                                                                                            <  1:1011543/149‑1 (MQ=255)
             gAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACa                                                                                                                                         <  1:814082/149‑1 (MQ=255)
               gCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACAt                                                                                                                                        <  4:513627/148‑1 (MQ=255)
                  aCTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGtt                                                                                                                                    <  2:1018920/149‑1 (MQ=255)
                  aCTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGt                                                                                                                                     >  4:278526/1‑148 (MQ=255)
                    tGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTa                                                                                                                                   >  4:62261/1‑148 (MQ=255)
                        cGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCttt                                                                                                                              <  1:437965/149‑1 (MQ=255)
                         gCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCttt                                                                                                                              <  3:843797/148‑1 (MQ=255)
                          cAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGt                                                                                                                            <  1:889617/149‑1 (MQ=255)
                              cGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGATCATTCGTTATTTAATTCAGAAGTAAACCCTCATTCCCGCTGTGACCGACAGAGGGAACATCGTTATCTTTGTATc                                                                                                                         >  3:893583/1‑148 (MQ=255)
                               gATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCt                                                                                                                                <  2:419632/140‑1 (MQ=255)
                               gATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCt                                                                                                                                >  1:419632/1‑140 (MQ=255)
                                aTCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATcaca                                                                                                                      >  1:530457/1‑149 (MQ=255)
                                aTCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATcac                                                                                                                       >  4:91594/1‑148 (MQ=255)
                                 tCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATcaca                                                                                                                      >  3:886737/1‑148 (MQ=255)
                                   aataatATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAg                                                                                                                   >  2:489034/1‑149 (MQ=255)
                                   aataatATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAg                                                                                                                   >  1:993064/1‑149 (MQ=255)
                                        tatTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGAtttt                                                                                                              >  1:280977/1‑149 (MQ=255)
                                         atTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGGGTCACAGCGGGaatgagg                                                                                                                                      >  2:122644/1‑119 (MQ=39)
                                         atTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGGGTCACAGCGGGaatgagg                                                                                                                                      <  1:122644/124‑6 (MQ=39)
                                           tCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTa                                                                                                                                   >  3:595698/1‑125 (MQ=255)
                                             cACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTa                                                                                                                                   >  2:468046/1‑123 (MQ=255)
                                             cACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTa                                                                                                                                   <  1:468046/123‑1 (MQ=255)
                                             cACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCg                                                                                                         >  1:119057/1‑149 (MQ=255)
                                                     gcgcGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCt                                                                                                                                <  2:507941/118‑1 (MQ=255)
                                                       gcgTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTcc                                                                                               >  2:886900/1‑149 (MQ=255)
                                                           aaTCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTAtt                                                                                           >  1:1074144/1‑149 (MQ=255)
                                                             tCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCt                                                                                         >  2:559814/1‑149 (MQ=255)
                                                              cACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCt                                                                                         >  4:111963/1‑148 (MQ=255)
                                                               aCGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCttt                                                                                       >  1:585134/1‑149 (MQ=255)
                                                               aCGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCttt                                                                                       >  1:284968/1‑149 (MQ=255)
                                                                cGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCtttt                                                                                      <  1:548432/149‑1 (MQ=255)
                                                                 ggCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCgg                                                                                                                                              >  1:162035/1‑92 (MQ=255)
                                                                 ggCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCgg                                                                                                                                              <  2:162035/92‑1 (MQ=255)
                                                                     ataCGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGt                                                                                  <  4:10128/148‑1 (MQ=255)
                                                                                gATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCagga                                                                      >  2:1022791/1‑149 (MQ=255)
                                                                                gATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCagga                                                                      <  2:530457/149‑1 (MQ=255)
                                                                                gATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCagg                                                                       >  3:797430/1‑148 (MQ=255)
                                                                                gATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCagg                                                                       <  3:774687/148‑1 (MQ=255)
                                                                                    ttCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGtt                                                                  >  1:103118/1‑149 (MQ=255)
                                                                                     tCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGa                                                                                                                  >  1:559896/1‑100 (MQ=255)
                                                                                     tCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGa                                                                                                                  <  2:559896/100‑1 (MQ=255)
                                                                                     tCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGtt                                                                  >  4:137557/1‑148 (MQ=255)
                                                                                     tCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGtt                                                                  <  3:674272/148‑1 (MQ=255)
                                                                                      cAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGttt                                                                 <  4:713646/148‑1 (MQ=255)
                                                                                      cAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTc                                                                >  2:125533/1‑149 (MQ=255)
                                                                                      cAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTc                                                                >  2:712492/1‑149 (MQ=255)
                                                                                       aGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTc                                                                <  1:1043144/148‑1 (MQ=255)
                                                                                       aGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTc                                                                >  2:1043144/1‑148 (MQ=255)
                                                                                         cAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGa                                                                              <  4:243697/132‑1 (MQ=255)
                                                                                         cAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGa                                                                              >  3:243697/1‑132 (MQ=255)
                                                                                         cAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTAt                                                             <  1:1022791/149‑1 (MQ=255)
                                                                                         cAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTAt                                                             <  1:66688/149‑1 (MQ=255)
                                                                                             aCGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTAtgtgt                                                         <  2:156834/149‑1 (MQ=255)
                                                                                               gggCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTgg                                                       >  1:713052/1‑149 (MQ=255)
                                                                                                  ccGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGAc                                                     >  4:150489/1‑148 (MQ=255)
                                                                                                    gACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCt                                                  <  1:559814/149‑1 (MQ=255)
                                                                                                     aCCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTAtgtg                                                          <  4:791470/140‑1 (MQ=255)
                                                                                                     aCCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTAtgtg                                                          >  3:791470/1‑140 (MQ=255)
                                                                                                     aCCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCt                                                  >  4:69851/1‑148 (MQ=255)
                                                                                                      ccATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCttt                                                <  1:509005/149‑1 (MQ=255)
                                                                                                        aTTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTTTTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGc                                              <  2:175963/149‑1 (MQ=255)
                                                                                                         ttCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGc                                                                                                                                                >  4:661244/1‑50 (MQ=255)
                                                                                                         ttCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGc                                                                                                                                                <  3:661244/50‑1 (MQ=255)
                                                                                                         ttCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCa                                                                                    >  2:170249/1‑110 (MQ=255)
                                                                                                         ttCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCa                                                                                    <  1:170249/110‑1 (MQ=255)
                                                                                                            gTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCaa                                          >  1:653868/1‑149 (MQ=255)
                                                                                                             ttATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATTGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCaa                                          >  3:854540/1‑148 (MQ=255)
                                                                                                             ttATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCaa                                          <  3:278526/148‑1 (MQ=255)
                                                                                                              tATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAAt                                         >  4:448063/1‑148 (MQ=255)
                                                                                                                tttAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATg                                                                               <  3:732089/108‑1 (MQ=255)
                                                                                                                tttAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATg                                                                               >  4:732089/1‑108 (MQ=255)
                                                                                                                 ttAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATccc                                      >  4:250585/1‑148 (MQ=255)
                                                                                                                   aaTTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCaa                                    <  4:820445/148‑1 (MQ=255)
                                                                                                                        aGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGt                              >  2:1066461/1‑149 (MQ=255)
                                                                                                                           aGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGa                            <  3:62261/148‑1 (MQ=255)
                                                                                                                           aGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAAGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGa                            <  3:604388/148‑1 (MQ=255)
                                                                                                                                tCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTa                      >  1:345533/1‑149 (MQ=255)
                                                                                                                                  ccTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTAcc                    >  1:1067172/1‑149 (MQ=255)
                                                                                                                                    atgttCCCGCTGTGGGTCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCCTTGCCCAATCCCAAACGCGTGAAGATTACCtt                  >  1:1064649/4‑149 (MQ=255)
                                                                                                                                      aTTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATc                                        <  1:358090/125‑1 (MQ=255)
                                                                                                                                      aTTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATc                                        >  2:358090/1‑125 (MQ=255)
                                                                                                                                         cccGCTGTGGGTCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTgcgc              >  1:259027/1‑148 (MQ=255)
                                                                                                                                         cccGCTGTGGGTCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTgcgc              <  2:259027/148‑1 (MQ=255)
                                                                                                                                         cccGCTGTGGGTCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCt             >  1:195508/1‑149 (MQ=255)
                                                                                                                                          ccGCTGTGGGTCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCt             >  3:393607/1‑148 (MQ=255)
                                                                                                                                          ccGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCtt            >  1:1032233/1‑149 (MQ=255)
                                                                                                                                          ccGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCtt            >  2:851100/1‑149 (MQ=255)
                                                                                                                                           cGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTtc           <  2:653868/149‑1 (MQ=255)
                                                                                                                                             cTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTtctc         <  2:807583/149‑1 (MQ=255)
                                                                                                                                                tgACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTcgcg      >  1:181937/1‑149 (MQ=255)
                                                                                                                                                    ccACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAg  >  1:294866/1‑149 (MQ=255)
                                                                                                                                                    ccACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAg  >  2:965005/1‑149 (MQ=255)
                                                                                                                                                    ccACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAg  >  1:434020/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCCTGGAAGTGGTGAGCGACTGTCCGCAGTCGATCAATAATATTCCACTGGATGCGCGTAATCACGGCTATACGGTGCTGGATATTCAGCAAGACGGGCCGACCATTCGTTATTTAATTCAGAAGTAATCCCTCATTCCCGCTGTGACCCACAGCGGGAACATCGTTACCTTTGTATCACATAGATTTTACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGTGTGGACGCTTTGCCCAATCCCAAACGCGTGAAGATTACCTTGCGCTTCTCGCGGAAG  >  NC_000913/2009585‑2009881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: