Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,305,209 G→A 19.1% L516L (CTG→TTG)  mqo ← malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,305,2090GA19.1% 149.9 / 29.6 68L516L (CTG→TTG) mqomalate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base G (42/13);  new base A (9/4);  total (51/17)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.80e-01

ATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCA  >  NC_000913/2305076‑2305355
                                                                                                                                     |                                                                                                                                                  
aTCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTa                                                                                                                                     >  2:1060288/1‑149 (MQ=255)
aTCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTa                                                                                                                                     >  2:473394/1‑149 (MQ=255)
  ccGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTAc                                                                                                                                    >  3:173397/1‑148 (MQ=255)
        tACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAACACTTCGCTGGTGTACTGCAAc                                                                                                                              >  4:739687/1‑148 (MQ=255)
           cATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGAGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTcgc                                                                                                                          >  1:703884/1‑149 (MQ=255)
            ntGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTcgc                                                                                                                          >  3:18333/2‑148 (MQ=255)
                   aTGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCAGGTGTACTGCAACTCGCGTTCTGtt                                                                                                                  <  1:381584/149‑1 (MQ=255)
                       ggCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTa                                                                                                                                     <  4:1022702/126‑1 (MQ=255)
                       ggCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTa                                                                                                                                     >  3:1022702/1‑126 (MQ=255)
                             tCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGt                                                                                                                        <  2:375802/133‑1 (MQ=255)
                             tCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGt                                                                                                                        >  1:375802/1‑133 (MQ=255)
                                 tACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCAc                                                                                                     >  3:535351/1‑148 (MQ=255)
                                    aaCGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTAGTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAACACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGcc                                                                                                            <  4:760756/138‑2 (MQ=255)
                                    aaCGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAACACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCt                                                                                                            >  3:760756/1‑138 (MQ=255)
                                    aaCGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAACACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGtt                                                                                                 >  1:164754/1‑149 (MQ=255)
                                     aCGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGtt                                                                                                 >  4:423081/1‑148 (MQ=255)
                                     aCGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGtt                                                                                                 >  3:824247/1‑148 (MQ=255)
                                      cGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAACACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCa                                                                                               >  2:787433/1‑149 (MQ=255)
                                        cAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAg                                                                                              >  3:445347/1‑148 (MQ=255)
                                                 ccACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTcc                                                                                     >  3:241402/1‑148 (MQ=255)
                                                 ccACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAACACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTcc                                                                                     >  4:833391/1‑148 (MQ=255)
                                                      tcttTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAg                                                                                >  3:181829/1‑148 (MQ=255)
                                                      tcttTTTGAACGGGTTGCGGTTTCAACTGCGGTTACGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAg                                                                                >  4:1049274/1‑148 (MQ=255)
                                                        tttttGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTc                                                                                                >  2:423066/1‑130 (MQ=255)
                                                        tttttGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTc                                                                                                >  2:571540/1‑130 (MQ=255)
                                                        tttttGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTc                                                                                                <  1:571540/130‑1 (MQ=255)
                                                        tttttGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTc                                                                                                <  1:423066/130‑1 (MQ=255)
                                                        tttttGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACg                                                                             >  2:94671/1‑149 (MQ=255)
                                                               aCGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGAt                                                                      >  2:616681/1‑149 (MQ=255)
                                                                cGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATc                                                                     >  1:765303/1‑149 (MQ=255)
                                                                    ttGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCt                                                                  <  4:173397/148‑1 (MQ=255)
                                                                        ggTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCa                                                              <  3:1049274/148‑1 (MQ=255)
                                                                          tttCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACGTCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACg                                                           >  1:942237/1‑149 (MQ=255)
                                                                           ttCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAACACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACg                                                           >  3:728686/1‑148 (MQ=255)
                                                                                cTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAACACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCt                                                     <  2:164754/149‑1 (MQ=255)
                                                                                 tGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAACACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTg                                                    >  2:967043/1‑149 (MQ=255)
                                                                                            cGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCggg                                          >  4:995316/1‑148 (MQ=255)
                                                                                             gTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCt                                        >  1:275608/1‑149 (MQ=255)
                                                                                              tACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTg                                       >  2:940305/1‑149 (MQ=255)
                                                                                               aCTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTgg                                      >  1:228975/1‑149 (MQ=255)
                                                                                                  aTCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGaa                                    >  3:882654/1‑148 (MQ=255)
                                                                                                     tgctgcCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAacac                                <  1:1060288/149‑1 (MQ=255)
                                                                                                      gctgcCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACg                               >  1:4103/1‑149 (MQ=255)
                                                                                                         gcctgcGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGAt                             <  4:181829/148‑1 (MQ=255)
                                                                                                         gcctgcGGCTTGTCGTAGTTCAACCCCAACACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGAt                             <  3:739687/148‑1 (MQ=255)
                                                                                                          cctgcGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATc                            >  4:766028/1‑148 (MQ=255)
                                                                                                             gcGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCAGCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGcc                         >  4:865844/1‑148 (MQ=255)
                                                                                                                 cTTGTCGTAGTTCAACCCCAACACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAAt                     <  4:728686/148‑1 (MQ=255)
                                                                                                                  ttGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGc                                         >  3:201905/1‑127 (MQ=255)
                                                                                                                  ttGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGc                                         <  4:201905/127‑1 (MQ=255)
                                                                                                                   tGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATAc                   >  4:322275/1‑148 (MQ=255)
                                                                                                                     tCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACCCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACtt                 <  4:241402/148‑1 (MQ=255)
                                                                                                                       gTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACttttt              >  2:215617/1‑149 (MQ=255)
                                                                                                                        tAGTTCAACCCCAACACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACttttt              >  3:773647/1‑148 (MQ=255)
                                                                                                                         aGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTc             >  3:100342/1‑148 (MQ=255)
                                                                                                                           ttCAACCCCAGCATTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGATCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTACCATTGCGAGCTTGAAACACGATAGCCCAATACTTTTTCcag          >  2:307711/1‑149 (MQ=255)
                                                                                                                           ttCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCcag          >  1:899054/1‑149 (MQ=255)
                                                                                                                            tCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCcag          >  3:718644/1‑148 (MQ=255)
                                                                                                                              aaCCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCcagcag       >  2:1025042/1‑149 (MQ=255)
                                                                                                                              aaCCCCAACACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCcagcag       >  1:937631/1‑149 (MQ=255)
                                                                                                                                ccccAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGAt     >  1:945637/1‑149 (MQ=255)
                                                                                                                                 cccAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAacac                                >  1:607070/1‑121 (MQ=255)
                                                                                                                                 cccAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAacac                                <  2:607070/121‑1 (MQ=255)
                                                                                                                                  ccAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTTCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTc   >  1:132921/1‑149 (MQ=255)
                                                                                                                                  ccAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTc             >  3:316164/1‑139 (MQ=255)
                                                                                                                                  ccAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTc             <  4:316164/139‑1 (MQ=255)
                                                                                                                                   cAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTc   >  3:921490/1‑148 (MQ=255)
                                                                                                                                   cAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCa  >  1:379019/1‑149 (MQ=255)
                                                                                                                                     |                                                                                                                                                  
ATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGCGTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCA  >  NC_000913/2305076‑2305355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: