Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,440,298 A→T 5.7% intergenic (‑178/+87) yfcJ ← / ← fabB putative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,440,2980AT 5.7% 350.7 / 7.1 102intergenic (‑178/+87)yfcJ/fabBputative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
Reads supporting (aligned to +/- strand):  ref base A (52/44);  new base T (3/3);  total (55/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGTT  >  NC_000913/2440156‑2440444
                                                                                                                                              |                                                                                                                                                  
tCCCCTCCTCATCTCTTTGGTTACAGGGTTCCGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGACATCGACAAAGCGGGAAGTTTGCCTGGAAGTGACTGCGATTGTCAATGATGTGAAAAAGGCAACCATCTGGTTcc                                                                                                                                              >  2:499728/1‑149 (MQ=255)
tCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTcc                                                                                                                                              >  2:629584/1‑149 (MQ=255)
tCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTc                                                                                                                                               <  3:721589/148‑1 (MQ=255)
 ccccGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTccc                                                                                                                                             <  1:651419/149‑1 (MQ=255)
 ccccGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTccc                                                                                                                                             <  2:782435/149‑1 (MQ=255)
   ccGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCtt                                                                                                                                           >  1:284897/1‑149 (MQ=255)
    cGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGCTGGTTCCCttt                                                                                                                                          <  1:561872/149‑1 (MQ=255)
     gTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCtttt                                                                                                                                         <  2:397311/149‑1 (MQ=255)
     gTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCtttt                                                                                                                                         <  2:180383/149‑1 (MQ=255)
     gTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCttt                                                                                                                                          <  3:716894/148‑1 (MQ=255)
      tctcATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCTGATGGTTCCCtttt                                                                                                                                         <  3:262283/148‑1 (MQ=255)
       ctcATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGGGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTg                                                                                                                                        <  3:736831/148‑1 (MQ=255)
          atttctctGGTTTCAGGGTTACCTGGCGTTTGCAGGATTTAGCGCGGACGTGTTTTCAGAAGGAAATCGACAAAGCGGCGAGTTTGCCTGGAACAGGCGGCGATTGTAAATGATGTGAAAGAGGGAACCACAAGGTTCCGTTTTGCGtt                                                                                                                                    <  1:142725/146‑1 (MQ=255)
          aTCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGtt                                                                                                                                    <  1:865662/149‑1 (MQ=255)
             tctctGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGGAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGt                                                                                                                                 >  1:744806/1‑149 (MQ=255)
               tctGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGc                                                                                                                               >  2:647806/1‑149 (MQ=255)
                ctGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGc                                                                                                                               >  3:272332/1‑148 (MQ=255)
                      tCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGc                                                                                                                        <  1:657551/149‑1 (MQ=255)
                          ggTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGc                                                                                                                     >  3:347602/1‑148 (MQ=255)
                            ttACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCAtt                                                                                                                  <  2:614524/149‑1 (MQ=255)
                            ttACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCAt                                                                                                                   >  4:839126/1‑148 (MQ=255)
                             tACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTg                                                                                                                 <  1:812840/149‑1 (MQ=255)
                               cGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTgg                                                                                                                <  3:197613/148‑1 (MQ=255)
                                 gTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGgcgc                                                                                                             >  1:409739/1‑149 (MQ=255)
                                  tGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGcgcg                                                                                                            >  2:690242/1‑149 (MQ=255)
                                        ggCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGt                                                                                                      >  2:897855/1‑149 (MQ=255)
                                            ggATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGc                                                                                                                     >  2:56266/1‑130 (MQ=255)
                                            ggATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGc                                                                                                                     <  1:56266/130‑1 (MQ=255)
                                            ggATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCgg                                                                                                   >  3:431157/1‑148 (MQ=255)
                                            ggATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGa                                                                                                  <  1:629584/149‑1 (MQ=255)
                                            ggATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGa                                                                                                  <  1:589359/149‑1 (MQ=255)
                                               tttAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATg                                                                                                >  3:596018/1‑148 (MQ=255)
                                                  aaCGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGCTGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGAc                                                                                            <  1:499728/149‑1 (MQ=255)
                                                  aaCGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGAc                                                                                            >  1:793461/1‑149 (MQ=255)
                                                  aaCGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGAc                                                                                            >  1:76245/1‑149 (MQ=255)
                                                     gcgTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCt                                                                                         <  1:750717/149‑1 (MQ=255)
                                                          cGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGgcgc                                                                                    <  2:665750/149‑1 (MQ=255)
                                                           gTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGcgcg                                                                                   >  2:896113/1‑149 (MQ=255)
                                                            tCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGt                                                                                  <  1:712572/149‑1 (MQ=255)
                                                            tCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGt                                                                                  >  1:811802/1‑149 (MQ=255)
                                                             cTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGt                                                                                  <  3:839126/148‑1 (MQ=255)
                                                              ttttCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCt                                                                                >  1:99004/1‑149 (MQ=255)
                                                                   aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           >  1:79236/1‑149 (MQ=255)
                                                                   aGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTc                                                                            <  3:366306/148‑1 (MQ=255)
                                                                    gAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTcc                                                                           >  3:229910/1‑148 (MQ=255)
                                                                    gAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCg                                                                          >  2:406363/1‑149 (MQ=255)
                                                                     aaGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTccc                                                                                                                                             >  2:9750/1‑81 (MQ=255)
                                                                     aaGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTccc                                                                                                                                             <  1:9750/81‑1 (MQ=255)
                                                                       ggAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGAcc                                                                       >  1:389365/1‑149 (MQ=255)
                                                                          aaTCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTAc                                                                    >  2:551032/1‑149 (MQ=255)
                                                                           aTCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTg                                                                                                                                        <  2:168862/80‑1 (MQ=255)
                                                                           aTCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTg                                                                                                                                        >  1:168862/1‑80 (MQ=255)
                                                                           aTCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACt                                                                   >  2:288597/1‑149 (MQ=255)
                                                                            tCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACt                                                                   >  4:477165/1‑148 (MQ=255)
                                                                                cAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGa                                                               <  3:74912/148‑1 (MQ=255)
                                                                                  aaGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAAtt                                                            >  1:935913/1‑149 (MQ=255)
                                                                                  aaGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAAt                                                             >  3:12210/1‑148 (MQ=255)
                                                                                   aGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAAtt                                                            <  3:782798/148‑1 (MQ=255)
                                                                                   aGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAAtt                                                            >  3:512883/1‑148 (MQ=255)
                                                                                     cGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTaa                                                          <  4:512883/148‑1 (MQ=255)
                                                                                     cGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAAt                                                         <  2:895052/149‑1 (MQ=255)
                                                                                     cGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAAt                                                         <  2:494816/149‑1 (MQ=255)
                                                                                       ggAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCt                                                                                         <  3:764332/115‑1 (MQ=255)
                                                                                       ggAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCt                                                                                         >  4:764332/1‑115 (MQ=255)
                                                                                       ggAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCt                                                       >  1:527635/1‑149 (MQ=255)
                                                                                        gAAGTTTGCCTGGAACTGGCGGCGATGGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCt                                                       >  3:707634/1‑148 (MQ=255)
                                                                                         aaGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCtt                                                      <  3:848275/148‑1 (MQ=255)
                                                                                           gTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCa                                                   <  2:109028/149‑1 (MQ=255)
                                                                                            tttGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGc                                                                 <  1:1038416/134‑1 (MQ=255)
                                                                                            tttGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGc                                                                 >  2:1038416/1‑134 (MQ=255)
                                                                                            tttGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCa                                                   >  4:58072/1‑148 (MQ=255)
                                                                                            tttGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAg                                                  >  2:7762/1‑149 (MQ=255)
                                                                                            tttGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGCTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCa                                                   >  3:373303/1‑148 (MQ=255)
                                                                                                ccTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTg                                              <  2:985246/149‑1 (MQ=255)
                                                                                                 cTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTg                                              <  4:12210/148‑1 (MQ=255)
                                                                                                  tGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTgc                                             >  4:270660/1‑148 (MQ=255)
                                                                                                   ggAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTgcg                                            >  3:145536/1‑148 (MQ=255)
                                                                                                    gAACTGGCGGCGATTGTCAATGATGTGGAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCa                                          <  2:744806/149‑1 (MQ=255)
                                                                                                             gcgATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGc                                  >  3:768375/1‑148 (MQ=255)
                                                                                                                aTTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTgg                              >  2:626278/1‑149 (MQ=255)
                                                                                                                   gTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTNGCGCGTCTACNCCGACCTACTGCGAATTANTCTTTCAGCTTNCGCATTACCAGCGTGGCGt                           <  1:910295/149‑1 (MQ=255)
                                                                                                                    tCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGtt                          >  2:286777/1‑149 (MQ=255)
                                                                                                                     cAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGtt                          >  3:3813/1‑148 (MQ=255)
                                                                                                                      aaTGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTgg                        >  2:281548/1‑149 (MQ=255)
                                                                                                                       atgatgTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGt                       <  1:286777/149‑1 (MQ=255)
                                                                                                                        tgatgTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTg                      <  1:288597/149‑1 (MQ=255)
                                                                                                                         gatgTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAAt                                                         <  1:281690/113‑1 (MQ=255)
                                                                                                                         gatgTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAAt                                                         >  2:281690/1‑113 (MQ=255)
                                                                                                                           tgtgAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTgccg                   >  1:245362/1‑149 (MQ=255)
                                                                                                                              gcaaaaGGGAACCTGATGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGCATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGccgccg                >  2:634405/3‑149 (MQ=255)
                                                                                                                               aaaaaGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccga               >  1:490711/1‑149 (MQ=255)
                                                                                                                                aaaaGGGAACCTGATGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaa              >  1:408509/1‑149 (MQ=255)
                                                                                                                                   aGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCgccgaagc            <  4:768375/148‑1 (MQ=255)
                                                                                                                                          cATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTg    <  1:690242/149‑1 (MQ=255)
                                                                                                                                          cATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTg    >  1:494745/1‑149 (MQ=255)
                                                                                                                                           aTCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGt   >  2:41733/1‑149 (MQ=255)
                                                                                                                                            tCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGtt  <  2:871540/149‑1 (MQ=255)
                                                                                                                                            tCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGt   >  4:119861/1‑148 (MQ=255)
                                                                                                                                             cAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGtt  <  4:272332/148‑1 (MQ=255)
                                                                                                                                             cAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGtt  <  3:218992/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TCCCCGTCTCATCTCTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTTTTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGCGGCGATTGTCAATGATGTGAAAAAGGGAACCATCAGGTTCCCTTTTGCGTTAGTGCCGGAGGCCGCATTGGCGCGTAACGTCGGATGCGACGCTGGCGCGTCTACTCCGACCTACTGCGAATTAATCTTTCAGCTTGCGCATTACCAGCGTGGCGTTGGTGCCGCCGAAGCCGAAGCTGTT  >  NC_000913/2440156‑2440444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: