Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,440,866 A→G 5.2% L247P (CTG→CCG) ‡ fabB ← 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,440,8660AG 5.2% 449.1 / 8.9 135L247P (CTG→CCG) ‡fabB3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
Reads supporting (aligned to +/- strand):  ref base A (68/60);  new base G (2/5);  total (70/65)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 136 total aligned reads displayed.

GTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCA  >  NC_000913/2440718‑2441011
                                                                                                                                                    |                                                                                                                                                 
 ttCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAg                                                                                                                                                  >  1:522766/1‑149 (MQ=255)
    aGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAgcgc                                                                                                                                               >  1:703330/1‑149 (MQ=255)
        aaTCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCTGGCGCgtgg                                                                                                                                           <  2:1055833/149‑2 (MQ=255)
        aaTCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCgtgt                                                                                                                                           >  2:365182/1‑149 (MQ=255)
        aaTCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCgtg                                                                                                                                            <  3:300436/148‑1 (MQ=255)
        aaTCGATTGGCGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCTGGCGCgtg                                                                                                                                            <  4:710933/148‑1 (MQ=255)
           cGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGtt                                                                                                                                          >  4:927887/1‑147 (MQ=255)
           cGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGtt                                                                                                                                          <  3:927887/147‑1 (MQ=255)
           cGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTcc                                                                                                                                        >  1:467094/1‑149 (MQ=255)
              ttGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGc                                                                                                                                     <  1:987811/149‑1 (MQ=255)
               tGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGct                                                                                                                                    >  2:760317/1‑149 (MQ=255)
               tGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGc                                                                                                                                     >  4:384484/1‑148 (MQ=255)
                ggggTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGctc                                                                                                                                   >  2:332092/1‑149 (MQ=255)
                 gggTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGctc                                                                                                                                   <  3:148091/148‑1 (MQ=255)
                  ggTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGctct                                                                                                                                  >  3:375555/1‑148 (MQ=255)
                     aTCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCaa                                                                                                                              <  2:830530/149‑1 (MQ=255)
                        aaCGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCa                                                                                                                           >  1:1036763/1‑149 (MQ=255)
                         aCGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCa                                                                                                                           >  3:351106/1‑148 (MQ=255)
                           gCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTa                                                                                                                        >  1:189833/1‑149 (MQ=255)
                            ccATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGCTCCAGCTCTTCAACCACTAc                                                                                                                       <  1:70368/149‑1 (MQ=255)
                                 cATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATAc                                                                                                                  <  1:1058221/149‑1 (MQ=255)
                                  aTCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATAcc                                                                                                                 <  2:423098/149‑1 (MQ=255)
                                  aTCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATAc                                                                                                                  <  3:776092/148‑1 (MQ=255)
                                     gCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATAccgc                                                                                                               <  3:362289/148‑1 (MQ=255)
                                      ccATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATAccgccg                                                                                                             >  1:884515/1‑149 (MQ=255)
                                        aTCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGGTGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATAccgccgcc                                                                                                           >  2:666966/1‑149 (MQ=255)
                                         tCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATAccgccgccg                                                                                                          >  2:749914/1‑149 (MQ=255)
                                              aTGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATAc                                                                                                                  >  2:161033/1‑136 (MQ=255)
                                              aTGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATAc                                                                                                                  <  1:161033/136‑1 (MQ=255)
                                                gCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTCTTCCAGCTATTCAACCACTACCATACCCCCGCCGCCAGCGa                                                                                                   >  2:162420/1‑149 (MQ=255)
                                                  aGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATa                                                                                                 >  1:768222/1‑149 (MQ=255)
                                                   gCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATa                                                                                                 <  3:4858/148‑1 (MQ=255)
                                                        cTGCGCCTTCGCCAGACGGAGCAACCATGTTTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGaaa                                                                                           <  1:698124/149‑1 (MQ=255)
                                                              cTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTc                                                                                      >  3:108902/1‑148 (MQ=255)
                                                                  gCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGt                                                                                 >  2:301984/1‑149 (MQ=255)
                                                                   ccAGACGTAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTg                                                                                >  2:573058/1‑149 (MQ=255)
                                                                        cGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGcgt                                                                           >  2:284981/1‑149 (MQ=255)
                                                                        cGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGcgt                                                                           <  2:522766/149‑1 (MQ=255)
                                                                         ggAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGcgt                                                                           <  3:178361/148‑1 (MQ=255)
                                                                            gCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTa                                                                       >  1:551079/1‑149 (MQ=255)
                                                                               aCCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGt                                                                    >  2:691715/1‑149 (MQ=255)
                                                                                ccATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTa                                                                   >  1:384930/1‑149 (MQ=255)
                                                                                 cATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTa                                                                   >  3:1043030/1‑148 (MQ=255)
                                                                                   tGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACgg                                                                <  2:467094/149‑1 (MQ=255)
                                                                                   tGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACg                                                                 >  3:600937/1‑148 (MQ=255)
                                                                                    gTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAgcgc                                                                                                                                               <  2:1064596/69‑1 (MQ=255)
                                                                                    gTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAgcgc                                                                                                                                               >  1:1064596/1‑69 (MQ=255)
                                                                                        gCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAgg                                                            >  4:369905/1‑148 (MQ=255)
                                                                                            cATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCtttt                                                       >  2:219246/1‑149 (MQ=255)
                                                                                              tCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGaaa                                                                                           <  2:603436/111‑1 (MQ=255)
                                                                                              tCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGaaa                                                                                           >  1:603436/1‑111 (MQ=255)
                                                                                              tCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACg                                                                 <  3:222248/137‑1 (MQ=255)
                                                                                                  agGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCggg                                                 <  2:352122/149‑1 (MQ=255)
                                                                                                  agGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCgg                                                  <  4:236469/148‑1 (MQ=255)
                                                                                                  agGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCgg                                                  <  4:1043030/148‑1 (MQ=255)
                                                                                                     ttGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGgtg                                              >  1:58818/1‑149 (MQ=255)
                                                                                                     ttGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGgt                                               >  4:327068/1‑148 (MQ=255)
                                                                                                       gcgcCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTc                                            >  1:833055/1‑149 (MQ=255)
                                                                                                         gcCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAg                                                                     >  2:976428/1‑122 (MQ=255)
                                                                                                         gcCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAg                                                                     <  1:976428/122‑1 (MQ=255)
                                                                                                          tcGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGt                                          <  3:93095/147‑1 (MQ=255)
                                                                                                          ccGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTcc                                                                                                                                        >  3:706479/1‑54 (MQ=255)
                                                                                                          ccGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGtt                                         <  1:365182/149‑1 (MQ=255)
                                                                                                          ccGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGt                                          <  3:735831/148‑1 (MQ=255)
                                                                                                             tAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGt                                       <  4:923669/148‑1 (MQ=255)
                                                                                                             tAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGt                                       >  4:171321/1‑148 (MQ=255)
                                                                                                               gCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTAtt                                    <  1:573058/149‑1 (MQ=255)
                                                                                                                 cAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGt                                                                                       <  4:747170/96‑1 (MQ=255)
                                                                                                                 cAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGt                                                                                       >  3:747170/1‑96 (MQ=255)
                                                                                                                 cAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCgggg                                                >  4:177512/1‑135 (MQ=255)
                                                                                                                 cAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCgggg                                                <  3:177512/135‑1 (MQ=255)
                                                                                                                 cAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGgtg                                              <  1:340953/137‑1 (MQ=255)
                                                                                                                 cAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTta                                  >  1:396319/1‑149 (MQ=255)
                                                                                                                  aaCGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCACCCACTACCATACCGCCGCCGCCAGCGATATCGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTtag                                 <  2:70269/149‑1 (MQ=255)
                                                                                                                  aaCGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTtag                                 >  1:890836/1‑149 (MQ=255)
                                                                                                                  aaCGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTta                                  <  3:693608/148‑1 (MQ=255)
                                                                                                                  aaCGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTta                                  <  4:338730/148‑1 (MQ=255)
                                                                                                                   aCGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTCCCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAg                                                                              <  4:664972/103‑1 (MQ=255)
                                                                                                                   aCGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTg                                                                                <  4:533551/101‑1 (MQ=255)
                                                                                                                   aCGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAg                                                                              >  3:664972/1‑103 (MQ=255)
                                                                                                                   aCGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTa                                                                       >  4:601577/1‑110 (MQ=255)
                                                                                                                   aCGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTa                                                                       <  3:601577/110‑1 (MQ=255)
                                                                                                                      aTTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTtagtag                              <  3:384484/148‑1 (MQ=255)
                                                                                                                      aTTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGa                             <  1:532190/149‑1 (MQ=255)
                                                                                                                         tCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACa                           <  3:694286/148‑1 (MQ=255)
                                                                                                                            gCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAgc                         <  1:779048/147‑1 (MQ=255)
                                                                                                                            gCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAgc                         >  2:779048/1‑147 (MQ=255)
                                                                                                                               tAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCAcc                    >  2:1036616/1‑149 (MQ=255)
                                                                                                                                aGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCAcc                    >  4:65845/1‑148 (MQ=255)
                                                                                                                                aGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTAAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCTTTGTATTTAGTAGACAGCGCAcc                    >  4:653669/1‑148 (MQ=255)
                                                                                                                                 gATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCa                  >  1:909700/1‑149 (MQ=255)
                                                                                                                                       tggaacacGCGCTGGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGt            >  2:587030/6‑149 (MQ=255)
                                                                                                                                           ccacGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGa         >  4:208874/1‑148 (MQ=255)
                                                                                                                                            cacGCGCTGGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTc           >  4:581998/1‑145 (MQ=255)
                                                                                                                                            cacGCGCTGGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTc           <  3:581998/145‑1 (MQ=255)
                                                                                                                                            cacGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAAc                                                                                          >  1:644206/1‑66 (MQ=255)
                                                                                                                                            cacGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGaa        <  3:835764/148‑1 (MQ=255)
                                                                                                                                             acGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAAtt      >  2:830945/1‑149 (MQ=255)
                                                                                                                                              cgcgCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAAtt      >  4:721335/1‑148 (MQ=255)
                                                                                                                                                  ccAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCa  <  3:327068/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GTTCAGGTAATCGATTGGGGTATCAACGCCATGCATCGCCATCTTCATGCAGCGTACTGCGCCTTCGCCAGACGGAGCAACCATGTCTGCACCATCAGAGGTTGCGCCGTAGCCAACGATTTCAGCATAGATGTGAGCACCACGCGCCAGCGCGTGTTCCAGCTCTTCAACCACTACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCA  >  NC_000913/2440718‑2441011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: