Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,532,268 T→C 8.1% intergenic (+44/‑141) cysZ → / → cysK sulfate transporter, sulfite inhibited/cysteine synthase A, O‑acetylserine sulfhydrolase A subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,532,2680TC 8.1% 383.8 / 18.2 123intergenic (+44/‑141)cysZ/cysKsulfate transporter, sulfite inhibited/cysteine synthase A, O‑acetylserine sulfhydrolase A subunit
Reads supporting (aligned to +/- strand):  ref base T (46/67);  new base C (5/5);  total (51/72)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

Only 100 of 128 total aligned reads displayed.

ACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAG  >  NC_000913/2532120‑2532413
                                                                                                                                                    |                                                                                                                                                 
aCGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGt                                                                                                                                                   <  2:808915/149‑1 (MQ=255)
  gatgatCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTgg                                                                                                                                                 >  2:1035149/1‑149 (MQ=255)
  gatgatCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTg                                                                                                                                                  <  4:589399/148‑1 (MQ=255)
     gatCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCtt                                                                                                                                              >  1:745429/1‑149 (MQ=255)
      atacagctgctTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGACCGATTGCTATCGCGATAATCACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCtt                                                                                                                                              >  4:902474/6‑148 (MQ=255)
      atCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTa                                                                                                                                             <  2:745429/149‑1 (MQ=255)
        cccGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTAt                                                                                                                                            <  4:303851/148‑1 (MQ=255)
        cccGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATg                                                                                                                                           <  1:826290/149‑1 (MQ=255)
          cGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATgcc                                                                                                                                         >  2:1076246/1‑149 (MQ=255)
              gctTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATgccgcc                                                                                                                                      <  4:178396/148‑1 (MQ=255)
              gctTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATgccgcc                                                                                                                                      <  3:995000/148‑1 (MQ=255)
              gctTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGCGGCATAAgccacc                                                                                                                                      <  4:729329/148‑1 (MQ=255)
               ctTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGcccc                                                                                                                                    >  2:992080/1‑149 (MQ=255)
               ctTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGccc                                                                                                                                     <  4:1010220/148‑1 (MQ=255)
                ttAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGcccc                                                                                                                                    >  3:946382/1‑148 (MQ=255)
                   aTCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGTTGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGGTTATGCCGCCCCTTa                                                                                                                                 >  3:990372/1‑148 (MQ=255)
                   aTCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTa                                                                                                                                 <  4:352868/148‑1 (MQ=255)
                    tCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTAtt                                                                                                                               >  2:416300/1‑149 (MQ=255)
                    tCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGCGGCATAAGCCACCCCtgttt                                                                                                                               <  1:418364/149‑5 (MQ=255)
                     cTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTAtt                                                                                                                               <  3:11930/148‑1 (MQ=255)
                       gTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTATACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTcc                                                                                                                             >  3:903263/1‑148 (MQ=255)
                        ttCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGt                                                                                                                                                   <  1:74404/125‑1 (MQ=255)
                        ttCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGt                                                                                                                                                   >  2:74404/1‑125 (MQ=255)
                         tcgtcatGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGAGAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCAt                                                                                                                           <  4:571854/145‑1 (MQ=255)
                           atcatGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCtt                                                                                                                        >  1:482144/1‑149 (MQ=255)
                            tcatGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCtt                                                                                                                        >  4:742612/1‑148 (MQ=255)
                            tcatGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTg                                                                                                                       >  2:355865/1‑149 (MQ=255)
                            tcatGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTg                                                                                                                       <  1:416300/149‑1 (MQ=255)
                              atGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCa                                                                                                                     <  1:738243/149‑1 (MQ=255)
                               tGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCa                                                                                                                     >  3:501509/1‑148 (MQ=255)
                                gCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCAt                                                                                                                    >  4:867777/1‑148 (MQ=255)
                                   cGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTc                                                                                                                 >  3:313067/1‑148 (MQ=255)
                                   cGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCa                                                                                                                <  1:992062/149‑1 (MQ=255)
                                           tttGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTc                                                                                                         <  3:185505/148‑1 (MQ=255)
                                               tgGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCtt                                                                                                     <  4:874200/148‑1 (MQ=255)
                                                ggCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTc                                                                                                    <  3:429496/148‑1 (MQ=255)
                                                ggCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTc                                                                                                    <  4:600676/148‑1 (MQ=255)
                                                ggCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTc                                                                                                    <  4:205498/148‑1 (MQ=255)
                                                  cgcgACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTg                                                                                                  >  4:527798/1‑148 (MQ=255)
                                                       cGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGtatat                                                                                             >  4:491133/1‑148 (MQ=255)
                                                          cGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGa                                                                                          <  4:338503/148‑1 (MQ=255)
                                                           gATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCCGTATATAGata                                                                                        <  2:183513/149‑1 (MQ=255)
                                                                 ggTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGata                                                                                        >  4:85937/1‑143 (MQ=255)
                                                                 ggTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGata                                                                                        <  3:85937/143‑1 (MQ=255)
                                                                 ggTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTa                                                                                   >  3:556628/1‑148 (MQ=255)
                                                                  gTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTaaa                                                                                 >  2:627513/1‑149 (MQ=255)
                                                                      aTTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCt                                                                             <  2:482144/149‑1 (MQ=255)
                                                                       ttGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTCAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCtt                                                                            <  1:188661/149‑1 (MQ=255)
                                                                        tGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCtt                                                                            <  3:777033/148‑1 (MQ=255)
                                                                        tGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTa                                                                           <  2:123228/149‑1 (MQ=255)
                                                                         gCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATATATATGCTAAATCCTTAc                                                                          <  2:816077/149‑1 (MQ=255)
                                                                         gCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTAc                                                                          <  2:1066990/149‑1 (MQ=255)
                                                                             tCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTcc                                                                      >  2:621521/1‑149 (MQ=255)
                                                                              cgcgATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTg                                                                                                  >  3:134632/1‑120 (MQ=255)
                                                                                    aaaCACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATAtt                                                               <  2:939008/149‑1 (MQ=255)
                                                                                       cacGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATtctc                                                            <  2:467337/149‑1 (MQ=255)
                                                                                       cacGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATtct                                                             <  3:101746/148‑1 (MQ=255)
                                                                                          gcgATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTg                                                          <  4:439269/148‑1 (MQ=255)
                                                                                          gcgATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGa                                                         <  1:525368/149‑1 (MQ=255)
                                                                                            gATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCata                                                                 >  2:703647/1‑139 (MQ=255)
                                                                                            gATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAg                                                        <  4:674378/148‑1 (MQ=255)
                                                                                            gATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTCGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCata                                                                 <  1:703647/139‑1 (MQ=255)
                                                                                                tgGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATtct                                                             >  4:355951/1‑139 (MQ=255)
                                                                                                   cGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTAt                                                 >  4:683797/1‑148 (MQ=255)
                                                                                                      tAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCt                                              <  3:491133/148‑1 (MQ=255)
                                                                                                        aCAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTAc                                            >  3:72791/1‑148 (MQ=255)
                                                                                                           agcTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCtgt                                        <  1:28292/147‑1 (MQ=255)
                                                                                                           aTCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCGCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCtgt                                        >  2:922281/1‑149 (MQ=255)
                                                                                                            tCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCtgtt                                       <  2:608831/149‑1 (MQ=255)
                                                                                                            tCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCtgtt                                       >  2:921779/1‑149 (MQ=255)
                                                                                                            tCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCtgt                                        <  4:269652/148‑1 (MQ=255)
                                                                                                             cTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCtgttg                                      <  1:1035149/149‑1 (MQ=255)
                                                                                                                   gTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACGTGTTGTATctc                                >  2:297165/1‑147 (MQ=255)
                                                                                                                   gTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATcc                                 >  3:609560/1‑148 (MQ=255)
                                                                                                                    ttATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCACTTCTGTATATAGATATGCTAAATCCTTACTTCTGCATATTCTCTGTGCGGGTATGCTATCTGTCGTATccc                                >  3:376635/1‑148 (MQ=255)
                                                                                                                     tATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCa                               >  4:255193/1‑148 (MQ=255)
                                                                                                                          tGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTc                          >  3:854408/1‑148 (MQ=255)
                                                                                                                            tAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCAt                        <  4:313067/148‑1 (MQ=255)
                                                                                                                            tAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCAt                        <  4:127888/148‑1 (MQ=255)
                                                                                                                            tAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATa                       <  1:831513/149‑1 (MQ=255)
                                                                                                                            tAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATa                       <  1:114364/149‑1 (MQ=255)
                                                                                                                              aaCCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATAc                      >  3:227765/1‑148 (MQ=255)
                                                                                                                               aCCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAg                    >  2:884109/1‑149 (MQ=255)
                                                                                                                                   tttGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTaa                <  1:297165/149‑1 (MQ=255)
                                                                                                                                   tttGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTa                 <  4:876498/148‑1 (MQ=255)
                                                                                                                                    ttGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTaa                <  4:245571/148‑1 (MQ=255)
                                                                                                                                         tgAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAg          <  1:992080/149‑1 (MQ=255)
                                                                                                                                          gAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAgg         <  1:929670/149‑1 (MQ=255)
                                                                                                                                          gAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAgg         >  2:954961/1‑149 (MQ=255)
                                                                                                                                            ataaGGGGCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATc                                  >  1:1075849/4‑122 (MQ=255)
                                                                                                                                            ataaGGGGCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATc                                  <  2:1075849/119‑1 (MQ=255)
                                                                                                                                              cAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGc        <  3:931396/146‑1 (MQ=255)
                                                                                                                                              cAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGc        >  4:931396/1‑146 (MQ=255)
                                                                                                                                              cAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCAt     <  1:430648/149‑1 (MQ=255)
                                                                                                                                               aGGGGCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATg    >  2:735848/1‑149 (MQ=255)
                                                                                                                                                ggggTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATg    <  4:169473/148‑1 (MQ=255)
                                                                                                                                                ggggTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATg    <  3:683797/148‑1 (MQ=255)
                                                                                                                                                ggggCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTc                          >  1:98294/1‑126 (MQ=255)
                                                                                                                                                ggggCGGCATAAGCCACCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGa   >  2:410888/1‑149 (MQ=255)
                                                                                                                                                  ggTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTACTGTATATAGATATGCTAAATCCTTACTCCCGCATACTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCCTACAGTTAAGGACAGGCCATGAg  <  4:175178/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACGATGATCCCGCTGCTTAATCTGTTCATCATGCCCGTTGCCGTTTGTGGCGCGACGGCGATGTGGGTCGATTGCTATCGCGATAAACACGCGATGTGGCGGTAACAATCTACCGGTTATTTTGTAAACCGTTTGTGTGAAACAGGGGTGGCTTATGCCGCCCCTTATTCCATCTTGCATGTCATTATTTCCCTTCTGTATATAGATATGCTAAATCCTTACTTCCGCATATTCTCTGAGCGGGTATGCTACCTGTTGTATCCCAATTTCATACAGTTAAGGACAGGCCATGAG  >  NC_000913/2532120‑2532413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: