BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,724,413	A→T	8.6%	S96C (AGC→TGC)	yfiM →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,724,413	0	A	T	8.6%	353.2 / 17.5	104	S96C (AGC→TGC)	yfiM	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base A (49/46); new base T (5/4); total (54/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

ATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCA  >  NC_000913/2724270‑2724560

aTCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCAc                                                                                                                                                 <  4:200629/148‑1 (MQ=255)

 tCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCg                                                                                                                                               >  1:520803/1‑149 (MQ=255)

   aGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCTTGCACCGGc                                                                                                                                             <  1:23572/149‑1 (MQ=255)

   aGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCgg                                                                                                                                              <  4:333717/148‑1 (MQ=255)

    ggggATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCt                                                                                                                                            >  1:437845/1‑149 (MQ=255)

     gggATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCta                                                                                                                                           >  1:91777/1‑149 (MQ=255)

     gggATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCt                                                                                                                                            >  3:707165/1‑148 (MQ=255)

      ggATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCta                                                                                                                                           >  3:601627/1‑148 (MQ=255)

        aTGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCtata                                                                                                                                         >  3:691495/1‑148 (MQ=255)

            gCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGt                                                                                                                                     <  4:500474/148‑1 (MQ=255)

            gCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTc                                                                                                                                    <  2:376063/149‑1 (MQ=255)

             ccGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTc                                                                                                                                    >  3:595068/1‑148 (MQ=255)

               gggATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTgg                                                                                                                                 <  1:240616/149‑1 (MQ=255)

                ggATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCta                                                                                                                                           >  2:806940/1‑138 (MQ=255)

                ggATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCta                                                                                                                                           <  1:806940/138‑1 (MQ=255)

                     gCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTg                                                                                                                           >  2:475318/1‑149 (MQ=255)

                      cAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTg                                                                                                                           <  4:695548/148‑1 (MQ=255)

                      cAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGa                                                                                                                          <  2:165027/149‑1 (MQ=255)

                        gTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAAc                                                                                                                             >  2:648352/1‑144 (MQ=255)

                        gTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAAc                                                                                                                             <  1:648352/144‑1 (MQ=255)

                            cATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCt                                                                                                                                   >  3:93282/1‑134 (MQ=255)

                            cATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCt                                                                                                                                   <  4:93282/134‑1 (MQ=255)

                                tttGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCTTGCACCGGcga                                                                                                                                           >  1:828484/1‑120 (MQ=255)

                                tttGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCTTGCACCGGcga                                                                                                                                           <  2:828484/122‑3 (MQ=255)

                                    gATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGa                                                                                                            <  2:377009/149‑1 (MQ=255)

                                     aTTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGa                                                                                                            <  4:936339/148‑1 (MQ=255)

                                      ttGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGAc                                                                                                           >  4:625352/1‑148 (MQ=255)

                                           gTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATc                                                                                                      >  4:830333/1‑148 (MQ=255)

                                            ttCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCtata                                                                                                                                         <  1:66348/112‑1 (MQ=255)

                                            ttCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCtata                                                                                                                                         >  2:66348/1‑112 (MQ=255)

                                             tctcTGTCAGTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATccc                                                                                                    >  3:109781/1‑148 (MQ=255)

                                              ctctGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCccct                                                                                                  >  2:507589/1‑149 (MQ=255)

                                               tctGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCccct                                                                                                  >  4:380607/1‑148 (MQ=255)

                                                 tGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCcccttcc                                                                                               <  1:1036580/149‑1 (MQ=255)

                                                  gTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCcccttccc                                                                                              >  1:265614/1‑149 (MQ=255)

                                                     aGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCttt                                                                                           >  2:128328/1‑149 (MQ=255)

                                                      gTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCttt                                                                                           <  3:378865/148‑1 (MQ=255)

                                                        ttGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGc                                                                                         >  4:1051217/1‑148 (MQ=255)

                                                         tGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGa                                                                                       <  1:507589/149‑1 (MQ=255)

                                                         tGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCAGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCg                                                                                        <  4:1029349/148‑1 (MQ=255)

                                                                 tCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCa                                                                                >  4:836835/1‑148 (MQ=255)

                                                                  cAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATc                                                                              <  1:1033828/149‑1 (MQ=255)

                                                                   aaaaGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCcccttccc                                                                                              <  1:21239/132‑1 (MQ=255)

                                                                   aaaaGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCcccttccc                                                                                              >  2:21239/1‑132 (MQ=255)

                                                                   aaaaGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCa                                                                             >  2:579033/1‑149 (MQ=255)

                                                                     aaGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTTGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCaaa                                                                           <  2:520803/149‑1 (MQ=255)

                                                                     aaGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCaaa                                                                           <  2:91777/149‑1 (MQ=255)

                                                                     aaGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCaaa                                                                           <  2:523617/149‑1 (MQ=255)

                                                                     aaGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCaaa                                                                           <  1:968933/149‑1 (MQ=255)

                                                                     aaGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCaa                                                                            <  3:812393/148‑1 (MQ=255)

                                                                     aaGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCaa                                                                            >  3:728553/1‑148 (MQ=255)

                                                                     aaGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGACACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCaa                                                                            <  4:601618/148‑1 (MQ=255)

                                                                      agagCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCAt                                                                                                       >  3:168058/1‑120 (MQ=255)

                                                                      agagCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCAt                                                                                                       <  4:168058/120‑1 (MQ=255)

                                                                        agCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGa                                                                         <  3:1051217/148‑1 (MQ=255)

                                                                         gCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGaaa                                                                       <  2:291264/149‑1 (MQ=255)

                                                                                 aTAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGaaa                                                                >  3:628573/1‑148 (MQ=255)

                                                                                      cgccCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGt                                                                                    >  3:864251/1‑123 (MQ=255)

                                                                                      cgccCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGt                                                                                    <  4:864251/123‑1 (MQ=255)

                                                                                       gccCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGccacc                                                         >  2:583654/1‑149 (MQ=255)

                                                                                         tccGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCTATGTAGCATCAAACAAACCAGAAACGCcaccac                                                       >  2:734299/2‑149 (MQ=255)

                                                                                         cccGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGccacca                                                        >  4:785239/1‑148 (MQ=255)

                                                                                         cccGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCcaccac                                                       <  1:1059375/149‑1 (MQ=255)

                                                                                               gggAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCAt                                                 <  1:38446/149‑1 (MQ=255)

                                                                                                ggAGCGGTTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATc                                                >  1:346373/1‑149 (MQ=255)

                                                                                                   gCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAg                                             >  1:857221/1‑149 (MQ=255)

                                                                                                   gCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAg                                             >  2:245349/1‑149 (MQ=255)

                                                                                                       ctggagctggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTc                                          >  3:230485/1‑148 (MQ=255)

                                                                                                               ggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCa                                  >  4:318124/1‑148 (MQ=255)

                                                                                                                 aaGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAACAAAACCAGAAACGCCACCACCGTCATCAAGGTCACATACCaga                                >  4:859750/1‑148 (MQ=255)

                                                                                                                  aGGATTTGACCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCagaag                              >  2:714067/1‑149 (MQ=255)

                                                                                                                   ggATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCagaag                              >  4:1058947/1‑148 (MQ=255)

                                                                                                                     aTTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAgaagaa                            <  3:35436/148‑1 (MQ=255)

                                                                                                                     aTTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGaagaag                           <  1:475318/149‑1 (MQ=255)

                                                                                                                       ttGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTcac                                        <  4:277641/134‑1 (MQ=255)

                                                                                                                       ttGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTcac                                        >  3:277641/1‑134 (MQ=255)

                                                                                                                       ttGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAgg                          <  4:107982/148‑1 (MQ=255)

                                                                                                                           ccTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGtt                     <  2:494189/149‑1 (MQ=255)

                                                                                                                            cTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGt                                           >  3:929403/1‑126 (MQ=255)

                                                                                                                            cTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGt                                           <  4:929403/126‑1 (MQ=255)

                                                                                                                             tGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGttt                    <  4:137504/148‑1 (MQ=255)

                                                                                                                              gggATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTTCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTcc                  >  2:519287/1‑149 (MQ=255)

                                                                                                                              gggATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCGTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTc                   >  3:886396/1‑148 (MQ=255)

                                                                                                                                 aTGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCa                 <  4:257969/147‑1 (MQ=255)

                                                                                                                                 aTGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCa                 >  3:257969/1‑147 (MQ=255)

                                                                                                                                   gTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTc              >  3:307245/1‑148 (MQ=255)

                                                                                                                                   atagCCGGTGCTTGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTc              >  3:1046087/4‑148 (MQ=255)

                                                                                                                                    tCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTcc             <  3:836835/148‑1 (MQ=255)

                                                                                                                                     cGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTa           <  2:265614/149‑1 (MQ=255)

                                                                                                                                     agCCGGTGCTTGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGaa                                                                        >  2:197709/2‑88 (MQ=255)

                                                                                                                                     agCCGGTGCTTGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGaa                                                                        <  1:197709/87‑1 (MQ=255)

                                                                                                                                      gCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTAt          <  1:128328/149‑1 (MQ=255)

                                                                                                                                       ccGGTGCTTGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCATCACGGCCATCaa                                              >  3:871364/1‑112 (MQ=255)

                                                                                                                                       ccGGTGCTTGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCaa                                              <  4:871364/112‑1 (MQ=255)

                                                                                                                                       ccGGTGCTTGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTAt          >  4:260195/1‑148 (MQ=255)

                                                                                                                                       ccGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTAtt         <  2:613099/149‑1 (MQ=255)

                                                                                                                                       ccGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTAt          <  3:859750/148‑1 (MQ=255)

                                                                                                                                        cGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCAtt               >  4:945635/1‑142 (MQ=255)

                                                                                                                                              aaGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCa  >  1:862762/1‑149 (MQ=255)

                                                                                                                                               aGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCa  >  4:431910/1‑148 (MQ=255)

ATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCA  >  NC_000913/2724270‑2724560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`