Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,753,986 A→T 7.4% intergenic (+40/+22) bamE → / ← ratB lipoprotein component of BamABCDE OM biogenesis complex/UPF0125 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,753,9860AT 7.4% 362.8 / 16.1 111intergenic (+40/+22)bamE/ratBlipoprotein component of BamABCDE OM biogenesis complex/UPF0125 family protein
Reads supporting (aligned to +/- strand):  ref base A (54/47);  new base T (3/5);  total (59/52)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 121 total aligned reads displayed.

CCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCT  >  NC_000913/2753838‑2754130
                                                                                                                                                    |                                                                                                                                                
ccAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCaa                                                                                                                                                  >  2:353483/1‑149 (MQ=255)
ccAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCaa                                                                                                                                                  <  2:27842/149‑1 (MQ=255)
 cAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCaa                                                                                                                                                  <  4:974532/148‑1 (MQ=255)
 cAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAAt                                                                                                                                                 <  2:888937/149‑1 (MQ=255)
   gCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGa                                                                                                                                               >  1:612861/1‑149 (MQ=255)
    cAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGa                                                                                                                                               <  3:590512/148‑1 (MQ=255)
       ccAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCAc                                                                                                                                           <  1:168784/149‑1 (MQ=255)
          ggTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCt                                                                                                                                         >  4:1024251/1‑148 (MQ=255)
           gTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttt                                                                                                                                       >  1:1068122/1‑149 (MQ=255)
            tCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCtttt                                                                                                                                      <  2:1068122/149‑1 (MQ=255)
              aTGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCttttt                                                                                                                                     >  3:496269/1‑148 (MQ=255)
               tGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCtttttt                                                                                                                                    <  3:543988/148‑1 (MQ=255)
                 aaGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCACCttttttaa                                                                                                                                  <  4:791098/148‑3 (MQ=255)
                 aaGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctg                                                                                                                                 >  2:563296/1‑149 (MQ=255)
                   ggTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgt                                                                                                                                >  3:263932/1‑148 (MQ=255)
                    gtgtAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtct                                                                                                                              <  1:390476/149‑1 (MQ=255)
                      gtAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgtgtg                                                                                                                             >  3:703468/1‑148 (MQ=255)
                       tAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCaa                                                                                                                                                  <  3:1017261/126‑1 (MQ=255)
                       tAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCaa                                                                                                                                                  >  3:31217/1‑126 (MQ=255)
                       tAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCaa                                                                                                                                                  >  4:1017261/1‑126 (MQ=255)
                       tAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCaa                                                                                                                                                  <  4:31217/126‑1 (MQ=255)
                       tAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTctgtctgt                                                                                                                            <  3:296560/148‑1 (MQ=255)
                         aCTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttat                                                                                                                         <  1:727730/149‑1 (MQ=255)
                           tCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttattt                                                                                                                       >  2:908630/1‑149 (MQ=255)
                           tCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttattt                                                                                                                       >  2:549444/1‑149 (MQ=255)
                            cAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGGGGTAACTAATAATAAAGTGGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttattt                                                                                                                       >  3:463880/1‑148 (MQ=255)
                              gCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttatttat                                                                                                                     >  3:716669/1‑148 (MQ=255)
                              gCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGttatttat                                                                                                                     <  4:496269/148‑1 (MQ=255)
                               cAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTtatttatt                                                                                                                    <  4:463880/148‑1 (MQ=255)
                               cAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTc                                                                                                                   <  1:868038/149‑1 (MQ=255)
                               cAAACGCTGACGCTGACCTTTAACAGTAACGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTtatttatt                                                                                                                    <  3:129930/148‑1 (MQ=255)
                                aaaCGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCg                                                                                                                  >  2:901336/1‑149 (MQ=255)
                                 aaCGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCg                                                                                                                  >  3:46268/1‑148 (MQ=255)
                                  acgctgacgctgACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGc                                                                                                                 >  4:339657/1‑148 (MQ=255)
                                     ctgacgctgacCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGAt                                                                                                             >  1:617344/1‑149 (MQ=255)
                                     ctgacgctgacCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGAt                                                                                                             <  2:893870/149‑1 (MQ=255)
                                        acgctgacCTTTAACAGTAGCGATGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGAtttt                                                                                                          >  2:949468/1‑149 (MQ=255)
                                               ccTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTGATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCt                                                                                                    >  4:1048958/1‑148 (MQ=255)
                                               ccTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTc                                                                                                   <  1:375614/149‑1 (MQ=255)
                                                 tttAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAGTGAGCACCTTTTTTCTGTCTGTTATTTATTCTCTGATTTTTCTGCTCg                                                                                                  >  3:437097/1‑148 (MQ=255)
                                                 tttAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCg                                                                                                  >  3:645741/1‑148 (MQ=255)
                                                    aaCAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTg                                                                                               <  4:933389/148‑1 (MQ=255)
                                                               tgtgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGct                                                                                    <  4:273839/148‑1 (MQ=255)
                                                                 tgtTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCtt                                                                                 >  1:678884/1‑149 (MQ=255)
                                                                   ttGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAg                                                                                      >  3:604241/1‑142 (MQ=255)
                                                                   ttGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAg                                                                                      <  4:604241/142‑1 (MQ=255)
                                                                   ttGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTc                                                                               >  2:628801/1‑149 (MQ=255)
                                                                     gACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCgg                                                                             >  2:29253/1‑149 (MQ=255)
                                                                      aCCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCgg                                                                             >  4:445292/1‑148 (MQ=255)
                                                                      aCCAATATTGATAACAAACCTGCGCTGAGTGGTAACGAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCgg                                                                             <  4:716669/148‑1 (MQ=255)
                                                                       ccAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGa                                                                            >  3:361319/1‑148 (MQ=255)
                                                                       ccAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGAt                                                                           <  1:549444/149‑1 (MQ=255)
                                                                       ccAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGAt                                                                           <  1:1025177/149‑1 (MQ=255)
                                                                       ccAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGAt                                                                           <  2:612861/149‑1 (MQ=255)
                                                                       ccAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGAt                                                                           >  2:284725/1‑149 (MQ=255)
                                                                        cAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATc                                                                          <  2:617344/149‑1 (MQ=255)
                                                                         aaTATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCAcc                                                                                                                                          <  4:193046/84‑1 (MQ=255)
                                                                         aaTATTGACAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCATTGAGCAcc                                                                                                                                          >  3:193046/1‑84 (MQ=255)
                                                                          atatTGATAACAAACGTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTATATGTTATTTATTCGCTGATTTTTCAGCTCGTTGCCTGCGAAGAACTTTCGGATCgg                                                                        >  2:830168/1‑149 (MQ=255)
                                                                            atTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTc                                                                               >  1:251267/1‑140 (MQ=255)
                                                                            atTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTc                                                                               <  2:251267/140‑1 (MQ=255)
                                                                             ttGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCaa                                                                     <  1:901336/149‑1 (MQ=255)
                                                                             ttGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCa                                                                      <  3:1024251/148‑1 (MQ=255)
                                                                              tGATAACAAACCTGCGCTGAGTGGTAACTAATGATAAAGTTGCGCTCAATGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCaa                                                                     <  4:183300/148‑1 (MQ=255)
                                                                                aTAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTCTTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATg                                                                   <  4:98382/148‑1 (MQ=255)
                                                                                aTAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATg                                                                   <  4:108071/148‑1 (MQ=255)
                                                                                  aaCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGAttttt                                                                                                         >  1:571897/1‑108 (MQ=255)
                                                                                   aCAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATgaga                                                                >  4:255267/1‑148 (MQ=255)
                                                                                    cAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTtatttatt                                                                                                                    >  4:213310/1‑95 (MQ=255)
                                                                                              cTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAAt                                                     <  4:645741/148‑1 (MQ=255)
                                                                                                        aCTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGAt                                          <  1:902278/149‑1 (MQ=255)
                                                                                                         cTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATc                                         >  1:50526/1‑149 (MQ=255)
                                                                                                         cTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATc                                         >  2:449740/1‑149 (MQ=255)
                                                                                                           aataatagAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGc                                       >  2:575979/1‑149 (MQ=255)
                                                                                                           aataataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGc                                       >  1:332426/1‑149 (MQ=255)
                                                                                                            ataataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGGTCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGc                                       <  3:831481/148‑1 (MQ=255)
                                                                                                            ataataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGc                                       >  3:756168/1‑148 (MQ=255)
                                                                                                              aataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTc                                                                                                        <  1:690477/81‑1 (MQ=255)
                                                                                                              aataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTc                                                                                                        >  2:690477/1‑81 (MQ=255)
                                                                                                              aataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCcat                                    >  1:453606/1‑149 (MQ=255)
                                                                                                               ataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgt                                                                                                                                >  1:827980/1‑56 (MQ=255)
                                                                                                               ataaAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTtctgt                                                                                                                                <  2:827980/56‑1 (MQ=255)
                                                                                                                   aGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATg                                <  4:390331/148‑1 (MQ=255)
                                                                                                                    gTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGc                               <  4:700253/148‑1 (MQ=255)
                                                                                                                        ctctCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCacac                           <  4:361319/148‑1 (MQ=255)
                                                                                                                        ctctCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACt                          >  2:839641/1‑149 (MQ=255)
                                                                                                                           tcAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTAt                        <  4:454459/148‑1 (MQ=255)
                                                                                                                              aaGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATgaga                                                                >  1:248846/1‑105 (MQ=255)
                                                                                                                              aaGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATgaga                                                                <  2:248846/105‑1 (MQ=255)
                                                                                                                                gACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTa                  <  1:581926/149‑1 (MQ=255)
                                                                                                                                 aCGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTa                  <  4:703468/148‑1 (MQ=255)
                                                                                                                                   gTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGtt               >  2:462105/1‑149 (MQ=255)
                                                                                                                                    ttAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTTATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGtt               <  3:1048958/148‑1 (MQ=255)
                                                                                                                                    ttAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGtt               >  3:415702/1‑148 (MQ=255)
                                                                                                                                       aaaaaGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGc           >  2:789843/1‑149 (MQ=255)
                                                                                                                                         aaaGGTGCTCATTGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCg         >  1:833776/1‑149 (MQ=255)
                                                                                                                                            ggTGCTCAATGAGCACCTTTTTTCTGTCTTTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGa       >  4:1023231/1‑148 (MQ=255)
                                                                                                                                              tGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACg     >  3:549806/1‑148 (MQ=255)
                                                                                                                                              tGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACg     >  3:607513/1‑148 (MQ=255)
                                                                                                                                                 tCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCt  >  4:931992/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CCAGCAACCAGGTCATGAAGGTGTAACTCAGCAAACGCTGACGCTGACCTTTAACAGTAGCGGTGTGTTGACCAATATTGATAACAAACCTGCGCTGAGTGGTAACTAATAATAAAGTTGCTCTCAAAGACGTTAAAAAAGGTGCTCAATGAGCACCTTTTTTCTGTCTGTTATTTATTCGCTGATTTTTCTGCTCGTTGCCTGCGAAGCTCTTTCGGATCGGCAATGAGAGGGCGATAAATCTCCACCCGATCGCCATCATGCACACTATCGCTTAGTTTTGCCGGACGGCT  >  NC_000913/2753838‑2754130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: