Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,873,919 C→T 9.7% G25D (GGT→GAT)  cysC ← adenosine 5'‑phosphosulfate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,873,9190CT 9.7% 337.9 / 20.9 114G25D (GGT→GAT) cysCadenosine 5'‑phosphosulfate kinase
Reads supporting (aligned to +/- strand):  ref base C (51/51);  new base T (4/7);  total (55/59)
Fisher's exact test for biased strand distribution p-value = 5.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.34e-01

Only 100 of 116 total aligned reads displayed.

ATCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCG  >  NC_000913/2873771‑2874067
                                                                                                                                                    |                                                                                                                                                    
aTCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGCGGCCGGTAAACCACAGCACTACACCACGATGAc                                                                                                                                                      >  2:596729/1‑149 (MQ=255)
   gCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgt                                                                                                                                                   >  1:1030146/1‑149 (MQ=255)
   gCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgt                                                                                                                                                   <  1:1067887/149‑1 (MQ=255)
   gCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCg                                                                                                                                                    <  3:764958/148‑1 (MQ=255)
    cTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACGCCGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgt                                                                                                                                                   >  4:688466/1‑148 (MQ=255)
    cTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgt                                                                                                                                                   >  4:521461/1‑148 (MQ=255)
     tGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtgg                                                                                                                                                 <  2:441313/149‑1 (MQ=255)
     tGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtgg                                                                                                                                                 >  1:868161/1‑149 (MQ=255)
      gCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCgtggt                                                                                                                                                <  2:274846/149‑1 (MQ=255)
      gCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCAACgtggt                                                                                                                                                <  2:763130/149‑1 (MQ=255)
      gCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtggt                                                                                                                                                <  2:6437/149‑1 (MQ=255)
         tAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACACCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                             <  2:400003/149‑1 (MQ=255)
          aaTCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                             <  4:701533/148‑1 (MQ=255)
          aaTCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTa                                                                                                                                             >  3:306512/1‑148 (MQ=255)
          aaTCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTa                                                                                                                                             >  3:435997/1‑148 (MQ=255)
           aTCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTAg                                                                                                                                            <  4:492319/148‑1 (MQ=255)
           aTCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGc                                                                                                                                           <  1:1077860/149‑1 (MQ=255)
             ccGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTc                                                                                                                                         <  2:868161/149‑1 (MQ=255)
             ccGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTc                                                                                                                                         >  2:105653/1‑149 (MQ=255)
                  gCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgtt                                                                                                                                    >  2:128681/1‑149 (MQ=255)
                  gCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgt                                                                                                                                     <  3:330670/148‑1 (MQ=255)
                   cGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgtt                                                                                                                                    >  4:418871/1‑148 (MQ=255)
                     aaCATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgtt                                                                                                                                 <  1:596729/149‑1 (MQ=255)
                      aCATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGttgttg                                                                                                                                >  2:52531/1‑149 (MQ=255)
                          tGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGcac                                                                                                                             <  3:521461/148‑1 (MQ=255)
                            tCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGt                                                                                                                          >  1:552947/1‑149 (MQ=255)
                             cGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTg                                                                                                                         >  2:240938/1‑149 (MQ=255)
                              gCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTg                                                                                                                         >  4:347440/1‑148 (MQ=255)
                                cATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGAc                                                                                                                       >  3:774004/1‑148 (MQ=255)
                                   ccAGCAGATACGTACTGACGCCGAGTCTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGa                                                                                                                   <  1:962514/149‑1 (MQ=255)
                                    cagcagATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGa                                                                                                                   <  4:400233/148‑1 (MQ=255)
                                     agcagATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATg                                                                                                                 <  1:481155/149‑1 (MQ=255)
                                            aCGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATg                                                                                                           <  4:600628/148‑1 (MQ=255)
                                              gTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCa                                                                                                        >  1:438875/1‑149 (MQ=255)
                                                  tGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGa                                                                                                                        >  2:958521/1‑129 (MQ=255)
                                                  tGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGa                                                                                                                        <  1:958521/129‑1 (MQ=255)
                                                  tGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAgac                                                                                                     >  4:655267/1‑148 (MQ=255)
                                                    aCGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAgacgac                                                                                                  >  1:385268/1‑149 (MQ=255)
                                                    aCGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAgacgac                                                                                                  <  2:438875/149‑1 (MQ=255)
                                                      gCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGt                                                                                                >  1:81952/1‑149 (MQ=255)
                                                      gCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGt                                                                                                >  2:603964/1‑149 (MQ=255)
                                                       ccGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGt                                                                                                <  3:677530/148‑1 (MQ=255)
                                                          aGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGtttt                                                                                             <  4:119104/148‑1 (MQ=255)
                                                          aGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTc                                                                                            <  2:316233/149‑1 (MQ=255)
                                                           gTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCg                                                                                           >  1:950524/1‑149 (MQ=255)
                                                            tttATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGt                                                                                          >  1:653830/1‑149 (MQ=255)
                                                            tttATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGt                                                                                          >  2:468398/1‑149 (MQ=255)
                                                             ttATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTc                                                                                         <  1:173129/149‑1 (MQ=255)
                                                              tATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTc                                                                                         >  4:442770/1‑148 (MQ=255)
                                                              tATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACAGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCa                                                                                        <  1:600161/149‑1 (MQ=255)
                                                               aTGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCAt                                                                                       <  2:413479/149‑1 (MQ=255)
                                                               aTGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCAt                                                                                       >  1:271772/1‑149 (MQ=255)
                                                                    aCGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAgc                                                                                  >  2:28926/1‑149 (MQ=255)
                                                                    aCGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAgc                                                                                  >  1:540058/1‑149 (MQ=255)
                                                                    aCGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAgc                                                                                  <  2:823611/149‑1 (MQ=255)
                                                                    aCGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAg                                                                                   >  3:271974/1‑148 (MQ=255)
                                                                       cctcctCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATg                                                                                                           >  4:530251/1‑121 (MQ=255)
                                                                       cctcctCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATg                                                                                                           <  3:530251/121‑1 (MQ=255)
                                                                       cctcctCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAgcgc                                                                                >  3:12915/1‑148 (MQ=255)
                                                                         tcctccAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGc                                                                                     <  1:225425/141‑1 (MQ=255)
                                                                         tcctccAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGc                                                                                     >  2:225425/1‑141 (MQ=255)
                                                                          cctccAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGCTGACCGTGGTGTAGCTCGGGTTGTTGCACAGTGACCGGTTGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCat                                                                             >  4:325896/1‑148 (MQ=255)
                                                                          cctccAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCat                                                                             <  4:366347/148‑1 (MQ=255)
                                                                           ctccAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCatta                                                                           >  1:36899/1‑149 (MQ=255)
                                                                                gcgcCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                             <  2:873221/78‑1 (MQ=38)
                                                                                gcgcCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                             >  1:873221/1‑78 (MQ=38)
                                                                                           aCCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCaaa                                                           <  1:1016917/149‑1 (MQ=255)
                                                                                               ttGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGTCGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcg                                                        <  4:668421/148‑1 (MQ=255)
                                                                                                        cGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTc                                                                                            >  2:200567/1‑103 (MQ=255)
                                                                                                        cGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTc                                                                                            <  1:200567/103‑1 (MQ=255)
                                                                                                              cGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGc                                         >  4:770011/1‑148 (MQ=255)
                                                                                                              cGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCg                                        >  1:231124/1‑149 (MQ=255)
                                                                                                               ggAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGTCGTTTTCGTCATGCAGCGCCattat                                                                          <  2:744419/114‑1 (MQ=255)
                                                                                                               ggAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCattat                                                                          >  1:744419/1‑114 (MQ=255)
                                                                                                                   agGCCGGTAAACCACAGCACTACGCCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAg                                   <  2:131520/149‑1 (MQ=255)
                                                                                                                    ggCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATCTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAg                                   >  4:348657/1‑148 (MQ=255)
                                                                                                                     gCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTg                                 <  1:824443/149‑1 (MQ=255)
                                                                                                                      ccGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTgg                                <  1:88337/149‑1 (MQ=255)
                                                                                                                      ccGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTg                                 <  3:770011/148‑1 (MQ=255)
                                                                                                                      ccGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTATTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTgg                                <  1:128681/149‑1 (MQ=255)
                                                                                                                       cGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTgg                                <  4:480326/148‑1 (MQ=255)
                                                                                                                        ggTAAACCACAGCACTACACCACGATGACCGTGTTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCACCCCAGTGCGGAAAGTGGc                               >  3:751973/1‑148 (MQ=255)
                                                                                                                         gTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGcga                             <  2:1005579/149‑1 (MQ=255)
                                                                                                                             aCCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGaa                         >  1:1041076/1‑149 (MQ=255)
                                                                                                                             aCCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGaa                         >  2:798701/1‑149 (MQ=255)
                                                                                                                              ccACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGaa                         <  3:348657/148‑1 (MQ=255)
                                                                                                                              ccACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGAGCGCCCCAGTGCGGAAAGTGGCGACGaa                         >  4:678681/1‑148 (MQ=255)
                                                                                                                               cacaGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAAcc                       >  1:292688/1‑149 (MQ=255)
                                                                                                                                 caGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCatt                                                                            <  4:236198/94‑1 (MQ=255)
                                                                                                                                 caGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCatt                                                                            >  3:236198/1‑94 (MQ=255)
                                                                                                                                  aGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCaga                    <  2:1041076/149‑1 (MQ=255)
                                                                                                                                  aGCACTACACCACGACGATCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCAATTATTTATCCCCCAGCAAACCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCaga                    <  2:896812/149‑1 (MQ=255)
                                                                                                                                   gCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCagag                   <  1:798701/149‑1 (MQ=255)
                                                                                                                                       tACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTgg                                <  2:1036177/132‑1 (MQ=255)
                                                                                                                                       tACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTgg                                >  1:1036177/1‑132 (MQ=255)
                                                                                                                                        acacCACGGTCATCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCAtt               >  3:839304/1‑148 (MQ=255)
                                                                                                                                        acacCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCAtt               >  3:884797/1‑148 (MQ=255)
                                                                                                                                            cacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAAtt          >  2:568929/1‑149 (MQ=255)
                                                                                                                                               gATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTcc        <  3:442770/148‑1 (MQ=255)
                                                                                                                                                    ccGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCg  <  2:950524/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCG  >  NC_000913/2873771‑2874067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: