Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,059,704 A→T 5.0% intergenic (‑3/‑49) yqfE ← / → argP pseudogene, LysR family/transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,059,7040AT 5.0% 404.6 / 7.4 116intergenic (‑3/‑49)yqfE/argPpseudogene, LysR family/transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
Reads supporting (aligned to +/- strand):  ref base A (46/64);  new base T (2/4);  total (48/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

AATAACGCCACGCCATTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGCATTACACAAT  >  NC_000913/3059556‑3059849
                                                                                                                                                    |                                                                                                                                                 
aaTAACGCCACGCCATTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACaaaa                                                                                                                                                   <  1:1056966/149‑1 (MQ=255)
  tAACGCCACGCCATTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAAtt                                                                                                                                                 <  1:226627/149‑1 (MQ=255)
  tAACGCCACGCCATTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAAt                                                                                                                                                  <  3:408062/148‑1 (MQ=255)
    acgccacgccATTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATtgt                                                                                                                                               <  1:179533/149‑1 (MQ=255)
    acgccacgccATTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATtg                                                                                                                                                <  4:42379/148‑1 (MQ=255)
     cgccacgccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATtgtg                                                                                                                                              <  1:801202/149‑1 (MQ=255)
     cgccacgccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATtgt                                                                                                                                               <  4:50137/148‑1 (MQ=255)
     cgccacgccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATtgt                                                                                                                                               <  4:263995/148‑1 (MQ=255)
         acgccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAATTTTGTGTCag                                                                                                                                          <  2:615267/149‑2 (MQ=255)
         acgccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCCAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAATTTTGTGTCa                                                                                                                                           <  4:94157/148‑1 (MQ=255)
         accccaTTAGACACGACCAGGGCGAGAACTCGCCGAGCCGAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCAt                                                                                                                                          >  2:204811/4‑149 (MQ=255)
           gccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAg                                                                                                                                        >  2:650996/1‑149 (MQ=255)
            ccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAg                                                                                                                                        <  3:843300/148‑1 (MQ=255)
            ccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGt                                                                                                                                       <  1:352832/149‑1 (MQ=255)
            ccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGt                                                                                                                                       <  2:1035302/149‑1 (MQ=255)
               ttCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCa                                                                                                                                    >  2:3489/1‑149 (MQ=255)
                 cAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAgg                                                                                                                                  <  2:848649/149‑1 (MQ=255)
                  aaCACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAgg                                                                                                                                  <  3:852351/148‑1 (MQ=255)
                  aaCACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGa                                                                                                                                 >  2:494802/1‑149 (MQ=255)
                  aaCACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGa                                                                                                                                 >  1:15450/1‑149 (MQ=255)
                   acacAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGa                                                                                                                                 <  3:63837/148‑1 (MQ=255)
                     acaACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGaaaa                                                                                                                              >  1:474941/1‑149 (MQ=255)
                      caACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGaaaa                                                                                                                              >  3:568712/1‑148 (MQ=255)
                        aCCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAg                                                                                                                            <  4:568712/148‑1 (MQ=255)
                           aGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCAtt                                                                                                                        >  1:1071054/1‑149 (MQ=255)
                             ggCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATtgt                                                                                                                                               <  4:411925/124‑1 (MQ=255)
                                  gAACGCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAgg                                                                                                                 >  2:641834/1‑149 (MQ=255)
                                      tCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCa                                                                                                             <  2:838318/149‑1 (MQ=255)
                                         cTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGa                                                                                                           <  4:68056/148‑1 (MQ=255)
                                            aGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTAc                                                                                                                     <  4:569878/135‑1 (MQ=255)
                                                     cATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAg                                                                                                                  >  1:952290/1‑129 (MQ=255)
                                                      aTTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGa                                                                                                           >  2:400370/1‑135 (MQ=255)
                                                      aTTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGa                                                                                                           <  1:400370/135‑1 (MQ=255)
                                                           cgcAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCACCCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGccc                                                                                        <  2:185053/149‑1 (MQ=255)
                                                            gcAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCg                                                                                       >  2:21300/1‑149 (MQ=255)
                                                                 tCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTa                                                                                  >  1:522058/1‑149 (MQ=255)
                                                                    cTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATtgtg                                                                                                                                              >  2:977569/1‑86 (MQ=255)
                                                                    cTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCa                                                                                                                   >  3:532438/1‑113 (MQ=255)
                                                                    cTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCa                                                                                                                   <  4:532438/113‑1 (MQ=255)
                                                                      gATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGaa                                                                             <  2:513109/149‑1 (MQ=255)
                                                                      gATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGaa                                                                             <  2:918870/149‑1 (MQ=255)
                                                                        tCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAgg                                                                                                                 >  3:473788/1‑111 (MQ=255)
                                                                        tCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAgg                                                                                                                 <  4:473788/111‑1 (MQ=255)
                                                                        tCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAAc                                                                            <  4:44393/148‑1 (MQ=255)
                                                                        tCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAAc                                                                            <  3:347562/148‑1 (MQ=255)
                                                                         cTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTAGGTATATGCAACCTGACACAAAATTGTGGCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACAt                                                                          <  2:1071054/149‑1 (MQ=255)
                                                                           tttAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTa                                                                        <  1:641834/149‑1 (MQ=255)
                                                                            ttAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTa                                                                        >  4:478367/1‑148 (MQ=255)
                                                                            ttAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTAc                                                                       <  2:522058/149‑1 (MQ=255)
                                                                            ttAAGCTGCGATTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTAGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTa                                                                        <  3:583164/148‑1 (MQ=255)
                                                                              aaGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAg                                                                     >  1:455216/1‑149 (MQ=255)
                                                                                 cTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGc                                                                                                                           <  3:751138/92‑1 (MQ=255)
                                                                                   gcgcTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCAc                                                                 >  3:87051/1‑148 (MQ=255)
                                                                                   gcgcTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACt                                                                >  1:845321/1‑149 (MQ=255)
                                                                                    cgcTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACt                                                                >  3:222201/1‑148 (MQ=255)
                                                                                     gcTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTgg                                                              >  2:84246/1‑149 (MQ=255)
                                                                                     gcTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTgg                                                              >  1:756049/1‑149 (MQ=255)
                                                                                       ttttAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAAt                                                                                                 >  2:894955/1‑112 (MQ=255)
                                                                                       ttttAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAAt                                                                                                 <  1:894955/112‑1 (MQ=255)
                                                                                       ttttAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGAt                                                            >  2:3648/1‑149 (MQ=255)
                                                                                           aaCCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCg                                                         <  4:9265/148‑1 (MQ=255)
                                                                                                        aaTGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACg                                           <  4:807083/148‑1 (MQ=255)
                                                                                                         aTGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGa                                          >  2:695036/1‑149 (MQ=255)
                                                                                                            ttACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAgg                                        <  4:401029/148‑1 (MQ=255)
                                                                                                            ttACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAgg                                        >  3:401029/1‑148 (MQ=255)
                                                                                                               cccGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGAtt                                     >  4:124377/1‑148 (MQ=255)
                                                                                                                ccgccgACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTg                                   >  1:1032993/1‑149 (MQ=255)
                                                                                                                 cgccgACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGTATGCGGTGATACGTGAACGAGGATTTg                                   <  3:71877/148‑1 (MQ=255)
                                                                                                                 cgccgACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTg                                   <  3:648621/148‑1 (MQ=255)
                                                                                                                 cgccgACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTg                                   >  4:851458/1‑148 (MQ=255)
                                                                                                                 cgccgACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGa                                  <  1:571841/149‑1 (MQ=255)
                                                                                                                   ccgACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAg                                 <  3:750415/148‑1 (MQ=255)
                                                                                                                       cGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAgcgcgc                            <  2:462708/149‑1 (MQ=255)
                                                                                                                       cGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAgcgcg                             <  3:48819/148‑1 (MQ=255)
                                                                                                                                tataTGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACaaaa                   <  1:454729/149‑1 (MQ=255)
                                                                                                                                 atatGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTg                                   <  4:1018145/132‑1 (MQ=255)
                                                                                                                                 atatGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTg                                   >  3:1018145/1‑132 (MQ=255)
                                                                                                                                 atatGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACaaaa                   <  3:287319/148‑1 (MQ=255)
                                                                                                                                   atGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGc                 <  4:222201/148‑1 (MQ=255)
                                                                                                                                    tGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCtg               <  1:1010869/149‑1 (MQ=255)
                                                                                                                                     gCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCGATGAAACGCCCGGACTACAGAACAGTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAACtg               <  4:144193/148‑1 (MQ=255)
                                                                                                                                     gCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCtgt              >  1:599565/1‑149 (MQ=255)
                                                                                                                                     gCAACCTGACACAAAATTGTGACATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCtg               >  4:814173/1‑148 (MQ=255)
                                                                                                                                      cAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCtgtg             <  1:300264/149‑1 (MQ=255)
                                                                                                                                       aaCCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGc            >  1:136195/1‑149 (MQ=255)
                                                                                                                                        aCCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGa                                                     >  2:963879/1‑107 (MQ=255)
                                                                                                                                        aCCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGa                                                     <  1:963879/107‑1 (MQ=255)
                                                                                                                                        aCCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGa                                       >  4:1021999/1‑121 (MQ=255)
                                                                                                                                        aCCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGa                                       <  3:1021999/121‑1 (MQ=255)
                                                                                                                                         tatGACACAATTTTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGCAt          >  1:558377/3‑149 (MQ=255)
                                                                                                                                         ccTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGcgcgcg                           >  4:148143/1‑132 (MQ=255)
                                                                                                                                         ccTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGcgcgcg                           <  3:148143/132‑1 (MQ=255)
                                                                                                                                          cTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACg                                                 >  4:294376/1‑109 (MQ=255)
                                                                                                                                          cTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACg                                                 <  3:294376/109‑1 (MQ=255)
                                                                                                                                           tGACACAATTTTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGCATTa        >  2:954509/1‑149 (MQ=255)
                                                                                                                                             acacAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGCATTaca      <  1:3489/149‑1 (MQ=255)
                                                                                                                                                caAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCaa                                                                                                  <  1:162475/54‑1 (MQ=255)
                                                                                                                                                caAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCaa                                                                                                  >  2:162475/1‑54 (MQ=255)
                                                                                                                                                caAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGCATTAcaca    >  3:44857/1‑148 (MQ=255)
                                                                                                                                                 aaaaTTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGCATTACACAAt  >  1:266595/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AATAACGCCACGCCATTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGCATTACACAAT  >  NC_000913/3059556‑3059849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: