Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,079,615 C→T 5.2% intergenic (‑285/+29) cmtB ← / ← tktA putative mannitol‑specific enzyme IIA component of PTS/transketolase 1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,079,6150CT 5.2% 315.3 / 4.2 96intergenic (‑285/+29)cmtB/tktAputative mannitol‑specific enzyme IIA component of PTS/transketolase 1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (55/36);  new base T (3/2);  total (58/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 101 total aligned reads displayed.

AGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAG  >  NC_000913/3079471‑3079763
                                                                                                                                                |                                                                                                                                                    
aGATATAACATAAAATGTCAATACGCATATCGTGATGCATATTACACAATTGATATTTATTTCATATAAATATTTGTGATCTACAACACGACTTATCTATTGCTTGTCCGCAAACGCACATATCAAGGTAATAAAAAAGGTCGCCGaa                                                                                                                                                   <  4:906336/148‑1 (MQ=255)
     taACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGAc                                                                                                                                              >  4:272449/1‑148 (MQ=255)
       acaacaAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtt                                                                                                                                           <  2:1044708/149‑1 (MQ=255)
         aacaaATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtttt                                                                                                                                         >  1:959002/1‑149 (MQ=255)
         aacaaATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtttt                                                                                                                                         >  1:810122/1‑149 (MQ=255)
          acaaATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCTTCTGCGACCttttt                                                                                                                                        <  2:752934/149‑1 (MQ=255)
          acaaATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtttt                                                                                                                                         >  4:1011128/1‑148 (MQ=255)
          acaaATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtttt                                                                                                                                         <  3:587273/148‑1 (MQ=255)
                gTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCg                                                                                                                                   >  4:489975/1‑148 (MQ=255)
                gTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCg                                                                                                                                   >  4:48973/1‑148 (MQ=255)
                     tACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATTTAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGc                                                                                                                             >  2:850530/1‑149 (MQ=255)
                        gCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTaa                                                                                                                          >  1:20533/1‑149 (MQ=255)
                                  aTGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGt                                                                                                                >  1:529549/1‑149 (MQ=255)
                                        atTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCttt                                                                                                           <  3:59609/148‑1 (MQ=255)
                                             aTAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCtt                                                                                                      >  4:254652/1‑148 (MQ=255)
                                             aTAATTGATATTTATTGAATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCttt                                                                                                     <  2:963417/149‑1 (MQ=255)
                                              tAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCttt                                                                                                     >  4:1053066/1‑148 (MQ=255)
                                                aTTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTa                                                                                                                       <  2:229282/128‑1 (MQ=255)
                                                aTTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTa                                                                                                                       >  1:229282/1‑128 (MQ=255)
                                                 ttGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGca                                                                                                 >  1:664951/1‑149 (MQ=255)
                                                     tatttattGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCt                                                                                                                                            <  2:254202/102‑1 (MQ=255)
                                                     tatttattGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCt                                                                                                                                            >  1:254202/1‑102 (MQ=255)
                                                     tatttattGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGcaacaa                                                                                             <  1:750271/149‑1 (MQ=255)
                                                       tttattGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCaacaac                                                                                            >  4:851282/1‑148 (MQ=255)
                                                         tattGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGt                                                                                          >  3:174590/1‑148 (MQ=255)
                                                          attGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGtt                                                                                         <  3:182177/148‑1 (MQ=255)
                                                          attGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTa                                                                                        <  1:800162/149‑1 (MQ=255)
                                                           ttGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTAt                                                                                       <  2:20533/149‑1 (MQ=255)
                                                            tGCATATAAATATTTTTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACg                                                                                           >  3:540214/1‑144 (MQ=255)
                                                            tGCATATAAATATTTTTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACg                                                                                           <  4:540214/144‑1 (MQ=255)
                                                               atatAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCaa                                                                                    >  4:758478/1‑148 (MQ=255)
                                                                tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAAc                                                                                   <  3:254652/148‑1 (MQ=255)
                                                                tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAAc                                                                                   <  3:305712/148‑1 (MQ=255)
                                                                aataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAAc                                                                                   >  3:676388/2‑148 (MQ=255)
                                                                 ataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACa                                                                                  <  3:1053066/148‑1 (MQ=255)
                                                                 ataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAg                                                                                 >  2:623497/1‑149 (MQ=255)
                                                                  taAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCACCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGt                                                                                >  2:977632/1‑149 (MQ=255)
                                                                    aaTATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTg                                                                               >  4:10991/1‑148 (MQ=255)
                                                                    aaTATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGa                                                                              >  1:449819/1‑149 (MQ=255)
                                                                     atatTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGa                                                                              <  4:451679/148‑1 (MQ=255)
                                                                      tatTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAg                                                                            <  2:810122/149‑1 (MQ=255)
                                                                               tCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAActct                                                                   >  1:513944/1‑149 (MQ=255)
                                                                                cTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAActct                                                                   >  3:817317/1‑148 (MQ=255)
                                                                                cTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCtt                                                                  >  2:529916/1‑149 (MQ=255)
                                                                                   caacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCa                                                                >  3:184399/1‑148 (MQ=255)
                                                                                     acacGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCaaa                                                              >  4:563891/1‑148 (MQ=255)
                                                                                     acacGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAc                                                             >  2:747670/1‑149 (MQ=255)
                                                                                         gCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAcagca                                                         <  1:747670/149‑1 (MQ=255)
                                                                                         gCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAcagca                                                         >  1:210556/1‑149 (MQ=255)
                                                                                         gCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAcagc                                                          <  4:174590/148‑1 (MQ=255)
                                                                                           cTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACagcagc                                                       >  2:990235/1‑149 (MQ=255)
                                                                                               tCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGctct                                                    >  3:341382/1‑148 (MQ=255)
                                                                                                cTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTg                                                   >  4:334343/1‑148 (MQ=255)
                                                                                                cTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGc                                                  <  1:529916/149‑1 (MQ=255)
                                                                                                  aTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGcc                                                 >  4:530664/1‑148 (MQ=255)
                                                                                                   ttGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCgg                                               >  1:776273/1‑149 (MQ=255)
                                                                                                   ttGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCACTTCTTTTGCTTTCGCAACAACGCTATCAACAGTGAAGCCGACCTCTTCAAACAGCAGCTCTGCCgg                                               >  2:694521/1‑149 (MQ=255)
                                                                                                    tGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGa                                              <  2:513944/149‑1 (MQ=255)
                                                                                                      cTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAg                                             <  4:185897/148‑1 (MQ=255)
                                                                                                       ttGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACATCGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCa                                           >  2:886502/1‑149 (MQ=255)
                                                                                                        tGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCa                                           >  3:791711/1‑148 (MQ=255)
                                                                                                        tGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCa                                           >  3:256830/1‑148 (MQ=255)
                                                                                                         gTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGa                                         <  2:529549/149‑1 (MQ=255)
                                                                                                          tCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGa                                         <  3:530664/148‑1 (MQ=255)
                                                                                                           ccGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAcagc                                                          <  3:928804/130‑1 (MQ=255)
                                                                                                           ccGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAcagc                                                          >  4:928804/1‑130 (MQ=255)
                                                                                                            cGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTc                                      >  1:738502/1‑149 (MQ=255)
                                                                                                              cAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCa                                     <  4:341382/148‑1 (MQ=255)
                                                                                                              cAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCAc                                    <  1:990235/149‑1 (MQ=255)
                                                                                                                aaCGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCAcc                                   >  4:921502/1‑148 (MQ=255)
                                                                                                                  cGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGaa                                <  1:623497/149‑1 (MQ=255)
                                                                                                                   ggACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGaa                                <  4:817728/148‑1 (MQ=255)
                                                                                                                   ggACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACAGAAg                               >  2:202250/1‑149 (MQ=255)
                                                                                                                    gACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAgg                              >  1:135302/1‑149 (MQ=255)
                                                                                                                      cATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAggt                             <  3:489975/148‑1 (MQ=255)
                                                                                                                      cATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAggt                             <  3:48973/148‑1 (MQ=255)
                                                                                                                        tatCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAggtgg                           >  4:912297/1‑148 (MQ=255)
                                                                                                                         atCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCCGCTCTGCCGGAGCAGATTCACCGAAggtggt                          <  4:676388/148‑1 (MQ=255)
                                                                                                                         atCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTc                         >  1:969724/1‑149 (MQ=255)
                                                                                                                          tCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTc                         >  3:611260/1‑148 (MQ=255)
                                                                                                                            aaGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATa                      >  1:941954/1‑149 (MQ=255)
                                                                                                                             aGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATa                      >  4:943110/1‑148 (MQ=255)
                                                                                                                             aGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATa                      >  4:256185/1‑148 (MQ=255)
                                                                                                                               gTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACcg                   >  2:1000951/1‑149 (MQ=255)
                                                                                                                                 aataaAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACcgac                 <  1:1037417/149‑1 (MQ=255)
                                                                                                                                 aataaAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACcgac                 <  2:738502/149‑1 (MQ=255)
                                                                                                                                 aataaAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACcgac                 >  2:511541/1‑149 (MQ=255)
                                                                                                                                     aaaaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGAt              >  3:893900/1‑148 (MQ=255)
                                                                                                                                     aaaaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGAt              <  4:184399/148‑1 (MQ=255)
                                                                                                                                     aaaaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATa             >  2:544318/1‑149 (MQ=255)
                                                                                                                                      aaaaGGTCGCTTCGGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAg            >  1:560417/1‑149 (MQ=255)
                                                                                                                                       aaaGGTCGCTTCGGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGAtt                                       <  2:57467/121‑1 (MQ=255)
                                                                                                                                       aaaGGTCGCTTCGGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGAtt                                       >  1:57467/1‑121 (MQ=255)
                                                                                                                                       aaaGGTCGCTTCGGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGc           >  2:776850/1‑149 (MQ=255)
                                                                                                                                          ggTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCAc         <  4:817317/148‑1 (MQ=255)
                                                                                                                                               ccGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTc                                                                 >  1:655293/1‑87 (MQ=255)
                                                                                                                                               ccGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTc                                                                 <  2:655293/87‑1 (MQ=255)
                                                                                                                                               ccGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTc    >  3:355774/1‑148 (MQ=255)
                                                                                                                                                cGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCa   <  3:334343/148‑1 (MQ=255)
                                                                                                                                                cGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAg  <  2:776273/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
AGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAG  >  NC_000913/3079471‑3079763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: