Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,098,515 G→C 7.6% intergenic (+112/+43) yggW → / ← yggM HemN family putative oxidoreductase/DUF1202 family putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,098,5150GC 7.6% 312.1 / 13.5 92intergenic (+112/+43)yggW/yggMHemN family putative oxidoreductase/DUF1202 family putative secreted protein
Reads supporting (aligned to +/- strand):  ref base G (46/39);  new base C (3/4);  total (49/43)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

TTAAATTCGCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTT  >  NC_000913/3098368‑3098659
                                                                                                                                                   |                                                                                                                                                
ttAAATTCGCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCggg                                                                                                                                                  >  3:749797/1‑148 (MQ=255)
  aaaTTCGCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTc                                                                                                                                               >  2:363924/1‑149 (MQ=255)
     ttCGCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCaa                                                                                                                                             <  4:217032/148‑1 (MQ=255)
     ttCGCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAAc                                                                                                                                            >  2:231206/1‑149 (MQ=255)
        gctgctGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAAcgcc                                                                                                                                         >  2:398438/1‑149 (MQ=255)
            ctgGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCAGGATGCGGCGTTGACCCCGCAt                                                                                                                                     <  1:813679/149‑1 (MQ=25)
             tgGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCAt                                                                                                                                     <  4:749797/148‑1 (MQ=255)
             tgGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCAt                                                                                                                                     >  3:92006/1‑148 (MQ=255)
             tgGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATc                                                                                                                                    >  1:784701/1‑149 (MQ=255)
             tgGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGCGTTGACCCCGCATc                                                                                                                                    <  1:584334/149‑1 (MQ=255)
               gAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCt                                                                                                                                  >  2:187107/1‑149 (MQ=255)
                 gCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTg                                                                                                                                 >  4:319155/1‑148 (MQ=255)
                  cTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGc                                                                                                                                >  4:655839/1‑148 (MQ=255)
                  cTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGc                                                                                                                                >  4:597120/1‑148 (MQ=255)
                   tttttCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGc                                                                                                                                <  4:254202/147‑1 (MQ=255)
                   tttttCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGc                                                                                                                                >  3:254202/1‑147 (MQ=255)
                   tttttCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGCGTTGACCCCGCATCCGGcaa                                                                                                                              <  2:993318/149‑3 (MQ=21)
                       tCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACaaa                                                                                                                          >  2:265775/1‑149 (MQ=255)
                         tGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAAt                                                                                                                         <  4:837002/148‑1 (MQ=255)
                             tGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGc                                                                                                                    <  2:481562/149‑1 (MQ=255)
                              gAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAGATCGTGc                                                                                                                    >  3:679406/1‑148 (MQ=255)
                              gAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGc                                                                                                                    <  3:715234/148‑1 (MQ=255)
                                gTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCAc                                                                                                                  >  4:594537/1‑148 (MQ=255)
                                 tAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACt                                                                                                                 >  3:878503/1‑148 (MQ=255)
                                    aCTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTatat                                                                                                             <  2:155401/149‑1 (MQ=255)
                                       tGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCa                                                                                                           >  3:819054/1‑148 (MQ=255)
                                       tGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCa                                                                                                           >  3:745632/1‑148 (MQ=255)
                                        gTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCaa                                                                                                          <  3:734088/148‑1 (MQ=255)
                                        gTATTGCCGGATGCGGCGTGAACGCCCTACCCAGCCCACATGTGGCAGCGGTTGTAGGTATGAGAAGACGCGCATGCGTCTCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGTATCCTCCTACAATTGGTGCACTATATCaa                                                                                                          <  4:655540/148‑1 (MQ=25)
                                           ttGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACtata                                                                                                              >  1:565540/1‑141 (MQ=255)
                                           ttGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACtata                                                                                                              <  2:565540/141‑1 (MQ=255)
                                            tGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAactta                                                                                                     <  2:8598/149‑1 (MQ=255)
                                            tGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAactta                                                                                                     <  2:3778/149‑1 (MQ=255)
                                             gCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAactta                                                                                                     <  3:750691/148‑1 (MQ=255)
                                              ccGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAacttact                                                                                                   <  2:253511/149‑1 (MQ=255)
                                                  aTGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAAt                                                                                               <  1:519455/149‑1 (MQ=255)
                                                    gcggcgTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATc                                                                                              <  3:319155/148‑1 (MQ=255)
                                                             aCGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTg                                                                                     <  4:387318/148‑1 (MQ=255)
                                                             aCGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACCATATCAAACTGACTTAATCACCCCATTg                                                                                     >  3:608845/1‑148 (MQ=255)
                                                                ccTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTgcgc                                                                                  >  3:460176/1‑148 (MQ=255)
                                                                  ttATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGcgccgc                                                                               >  1:707342/1‑149 (MQ=255)
                                                                  ttATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGcgccgc                                                                               >  1:257653/1‑149 (MQ=255)
                                                                     tCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgt                                                                            >  2:334870/1‑149 (MQ=255)
                                                                        aGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgt                                                                         >  2:1038820/1‑149 (MQ=255)
                                                                         gCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgtt                                                                        >  2:575965/1‑149 (MQ=255)
                                                                         gCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgtt                                                                        >  2:147176/1‑149 (MQ=255)
                                                                         gCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgtt                                                                        >  1:380838/1‑149 (MQ=255)
                                                                         gCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgt                                                                         >  3:926217/1‑148 (MQ=255)
                                                                          ccGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgtt                                                                        >  4:286196/1‑148 (MQ=255)
                                                                          ccGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTc                                                                       <  1:265775/149‑1 (MQ=255)
                                                                           cGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTc                                                                       <  4:395167/148‑1 (MQ=255)
                                                                           cGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCg                                                                      >  2:656023/1‑149 (MQ=255)
                                                                                tgtgGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTAt                                                                  <  4:926217/148‑1 (MQ=255)
                                                                                  tgGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCtt                                                               >  2:265094/1‑149 (MQ=255)
                                                                                  tgGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCtt                                                               >  2:554141/1‑149 (MQ=255)
                                                                                  tgGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCtt                                                               >  2:487207/1‑149 (MQ=255)
                                                                                     cAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCtttt                                                             <  4:70899/148‑1 (MQ=255)
                                                                                       gCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTc                                                          <  2:642401/149‑1 (MQ=255)
                                                                                         ggTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCCTCCTGCTACAAATCGTGCACTATATCAAACTTCCTTAATCACCCCAGTGCGCCGCAGTTGTTCGGTATCTTTTTTCCa                                                        <  2:335866/149‑1 (MQ=255)
                                                                                            tGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCg                                                     <  2:784701/149‑1 (MQ=255)
                                                                                              tAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGtt                                                   <  2:900160/149‑1 (MQ=255)
                                                                                                  tCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCAtt                                               >  1:622224/1‑149 (MQ=255)
                                                                                                      aTAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGc                                            <  4:43447/148‑1 (MQ=255)
                                                                                                      aTAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCt                                           >  1:574473/1‑149 (MQ=255)
                                                                                                         aGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTgg                                        >  2:317854/1‑149 (MQ=255)
                                                                                                         aGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTgg                                        <  1:1006256/149‑1 (MQ=255)
                                                                                                         aGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTgg                                        >  1:367012/1‑149 (MQ=255)
                                                                                                           aCGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGt                                       <  4:917967/148‑1 (MQ=255)
                                                                                                             gcgcAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGa                                    <  1:826338/149‑1 (MQ=255)
                                                                                                               gcAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGat                                   <  4:878503/148‑1 (MQ=255)
                                                                                                                          aTCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgt                                                                         <  4:67986/99‑1 (MQ=255)
                                                                                                                          aTCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgt                                                                         >  3:67986/1‑99 (MQ=255)
                                                                                                                          aTCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAAtttt                       <  1:334870/149‑1 (MQ=255)
                                                                                                                           tCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTtc                      >  1:519742/1‑149 (MQ=255)
                                                                                                                            cAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTtc                      >  3:660043/1‑148 (MQ=255)
                                                                                                                             aGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTtcat                    >  2:453112/1‑149 (MQ=255)
                                                                                                                              gACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACta                                                                                                                >  3:880285/1‑56 (MQ=255)
                                                                                                                              gACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACta                                                                                                                <  4:880285/56‑1 (MQ=255)
                                                                                                                                 gTTGATTGCCGGATGTGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCaaa                >  1:1001511/1‑149 (MQ=255)
                                                                                                                                    gATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAAt              >  3:824328/1‑148 (MQ=255)
                                                                                                                                      ttGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATcg            <  4:460176/148‑1 (MQ=255)
                                                                                                                                      ttGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATcg            >  3:493494/1‑148 (MQ=255)
                                                                                                                                         caggATGCGGCGTTGACCCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGa         >  4:693805/3‑148 (MQ=255)
                                                                                                                                           ggATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGc                                            >  1:728582/1‑111 (MQ=255)
                                                                                                                                           ggATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGc                                            <  2:728582/111‑1 (MQ=255)
                                                                                                                                           ggATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtt      >  2:426312/1‑149 (MQ=255)
                                                                                                                                             aTGCGGCGTTGACCCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTa                                              >  4:338124/1‑107 (MQ=255)
                                                                                                                                             aTGCGGCGTTGACCCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTa                                              <  3:338124/107‑1 (MQ=255)
                                                                                                                                             aTGCGGCGTTGACCCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGa                                    >  3:318644/1‑117 (MQ=255)
                                                                                                                                              tGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttt    <  3:655839/148‑1 (MQ=255)
                                                                                                                                               gCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTGTCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACttttt   <  3:277984/148‑1 (MQ=255)
                                                                                                                                                cGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttttt  <  4:1054861/148‑1 (MQ=255)
                                                                                                                                                cGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttttt  <  4:824328/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TTAAATTCGCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTT  >  NC_000913/3098368‑3098659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑