Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,098,519 A→G 7.5% intergenic (+116/+39) yggW → / ← yggM HemN family putative oxidoreductase/DUF1202 family putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,098,5190AG 7.5% 288.9 / 8.8 92intergenic (+116/+39)yggW/yggMHemN family putative oxidoreductase/DUF1202 family putative secreted protein
Reads supporting (aligned to +/- strand):  ref base A (46/39);  new base G (3/4);  total (49/43)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TTCGCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTA  >  NC_000913/3098373‑3098667
                                                                                                                                                  |                                                                                                                                                    
ttCGCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCaa                                                                                                                                                     <  4:217032/148‑1 (MQ=255)
ttCGCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAAc                                                                                                                                                    >  2:231206/1‑149 (MQ=255)
   gctgctGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAAcgcc                                                                                                                                                 >  2:398438/1‑149 (MQ=255)
       ctgGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCAGGATGCGGCGTTGACCCCGCAt                                                                                                                                             <  1:813679/149‑1 (MQ=25)
        tgGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCAt                                                                                                                                             <  4:749797/148‑1 (MQ=255)
        tgGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCAt                                                                                                                                             >  3:92006/1‑148 (MQ=255)
        tgGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATc                                                                                                                                            >  1:784701/1‑149 (MQ=255)
        tgGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGCGTTGACCCCGCATc                                                                                                                                            <  1:584334/149‑1 (MQ=255)
          gAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCt                                                                                                                                          >  2:187107/1‑149 (MQ=255)
            gCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTg                                                                                                                                         >  4:319155/1‑148 (MQ=255)
             cTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGc                                                                                                                                        >  4:597120/1‑148 (MQ=255)
             cTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGc                                                                                                                                        >  4:655839/1‑148 (MQ=255)
              tttttCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGc                                                                                                                                        <  4:254202/147‑1 (MQ=255)
              tttttCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGc                                                                                                                                        >  3:254202/1‑147 (MQ=255)
              tttttCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGCGTTGACCCCGCATCCGGcaa                                                                                                                                      <  2:993318/149‑3 (MQ=21)
                  tCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACaaa                                                                                                                                  >  2:265775/1‑149 (MQ=255)
                    tGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAAt                                                                                                                                 <  4:837002/148‑1 (MQ=255)
                        tGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGc                                                                                                                            <  2:481562/149‑1 (MQ=255)
                         gAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAGATCGTGc                                                                                                                            >  3:679406/1‑148 (MQ=255)
                         gAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGc                                                                                                                            <  3:715234/148‑1 (MQ=255)
                           gTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCAc                                                                                                                          >  4:594537/1‑148 (MQ=255)
                            tAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACt                                                                                                                         >  3:878503/1‑148 (MQ=255)
                               aCTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTatat                                                                                                                     <  2:155401/149‑1 (MQ=255)
                                  tGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCa                                                                                                                   >  3:745632/1‑148 (MQ=255)
                                  tGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCa                                                                                                                   >  3:819054/1‑148 (MQ=255)
                                   gTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCaa                                                                                                                  <  3:734088/148‑1 (MQ=255)
                                   gTATTGCCGGATGCGGCGTGAACGCCCTACCCAGCCCACATGTGGCAGCGGTTGTAGGTATGAGAAGACGCGCATGCGTCTCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGTATCCTCCTACAATTGGTGCACTATATCaa                                                                                                                  <  4:655540/148‑1 (MQ=25)
                                      ttGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACtata                                                                                                                      <  2:565540/141‑1 (MQ=255)
                                      ttGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACtata                                                                                                                      >  1:565540/1‑141 (MQ=255)
                                       tGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAactta                                                                                                             <  2:8598/149‑1 (MQ=255)
                                       tGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAactta                                                                                                             <  2:3778/149‑1 (MQ=255)
                                        gCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAactta                                                                                                             <  3:750691/148‑1 (MQ=255)
                                         ccGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAacttact                                                                                                           <  2:253511/149‑1 (MQ=255)
                                             aTGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAAt                                                                                                       <  1:519455/149‑1 (MQ=255)
                                               gcggcgTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATc                                                                                                      <  3:319155/148‑1 (MQ=255)
                                                        aCGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTg                                                                                             <  4:387318/148‑1 (MQ=255)
                                                        aCGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACCATATCAAACTGACTTAATCACCCCATTg                                                                                             >  3:608845/1‑148 (MQ=255)
                                                           ccTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTgcgc                                                                                          >  3:460176/1‑148 (MQ=255)
                                                             ttATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGcgccgc                                                                                       >  1:257653/1‑149 (MQ=255)
                                                             ttATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGcgccgc                                                                                       >  1:707342/1‑149 (MQ=255)
                                                                tCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgt                                                                                    >  2:334870/1‑149 (MQ=255)
                                                                   aGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgt                                                                                 >  2:1038820/1‑149 (MQ=255)
                                                                    gCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgtt                                                                                >  1:380838/1‑149 (MQ=255)
                                                                    gCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgtt                                                                                >  2:575965/1‑149 (MQ=255)
                                                                    gCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgtt                                                                                >  2:147176/1‑149 (MQ=255)
                                                                    gCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgt                                                                                 >  3:926217/1‑148 (MQ=255)
                                                                     ccGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgtt                                                                                >  4:286196/1‑148 (MQ=255)
                                                                     ccGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTc                                                                               <  1:265775/149‑1 (MQ=255)
                                                                      cGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTc                                                                               <  4:395167/148‑1 (MQ=255)
                                                                      cGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCg                                                                              >  2:656023/1‑149 (MQ=255)
                                                                           tgtgGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTAt                                                                          <  4:926217/148‑1 (MQ=255)
                                                                             tgGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCtt                                                                       >  2:265094/1‑149 (MQ=255)
                                                                             tgGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCtt                                                                       >  2:554141/1‑149 (MQ=255)
                                                                             tgGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCtt                                                                       >  2:487207/1‑149 (MQ=255)
                                                                                cAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCtttt                                                                     <  4:70899/148‑1 (MQ=255)
                                                                                  gCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTc                                                                  <  2:642401/149‑1 (MQ=255)
                                                                                    ggTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCCTCCTGCTACAAATCGTGCACTATATCAAACTTCCTTAATCACCCCAGTGCGCCGCAGTTGTTCGGTATCTTTTTTCCa                                                                <  2:335866/149‑1 (MQ=255)
                                                                                       tGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCg                                                             <  2:784701/149‑1 (MQ=255)
                                                                                         tAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGtt                                                           <  2:900160/149‑1 (MQ=255)
                                                                                             tCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCAtt                                                       >  1:622224/1‑149 (MQ=255)
                                                                                                 aTAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGc                                                    <  4:43447/148‑1 (MQ=255)
                                                                                                 aTAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCt                                                   >  1:574473/1‑149 (MQ=255)
                                                                                                    aGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTgg                                                <  1:1006256/149‑1 (MQ=255)
                                                                                                    aGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTgg                                                >  1:367012/1‑149 (MQ=255)
                                                                                                    aGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTgg                                                >  2:317854/1‑149 (MQ=255)
                                                                                                      aCGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGt                                               <  4:917967/148‑1 (MQ=255)
                                                                                                        gcgcAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGa                                            <  1:826338/149‑1 (MQ=255)
                                                                                                          gcAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGat                                           <  4:878503/148‑1 (MQ=255)
                                                                                                                     aTCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgt                                                                                 >  3:67986/1‑99 (MQ=255)
                                                                                                                     aTCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAgttgt                                                                                 <  4:67986/99‑1 (MQ=255)
                                                                                                                     aTCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAAtttt                               <  1:334870/149‑1 (MQ=255)
                                                                                                                      tCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTtc                              >  1:519742/1‑149 (MQ=255)
                                                                                                                       cAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTtc                              >  3:660043/1‑148 (MQ=255)
                                                                                                                        aGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTtcat                            >  2:453112/1‑149 (MQ=255)
                                                                                                                         gACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACta                                                                                                                        >  3:880285/1‑56 (MQ=255)
                                                                                                                         gACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACta                                                                                                                        <  4:880285/56‑1 (MQ=255)
                                                                                                                            gTTGATTGCCGGATGTGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCaaa                        >  1:1001511/1‑149 (MQ=255)
                                                                                                                               gATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAAt                      >  3:824328/1‑148 (MQ=255)
                                                                                                                                 ttGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATcg                    <  4:460176/148‑1 (MQ=255)
                                                                                                                                 ttGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATcg                    >  3:493494/1‑148 (MQ=255)
                                                                                                                                    caggATGCGGCGTTGACCCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGa                 >  4:693805/3‑148 (MQ=255)
                                                                                                                                      ggATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGc                                                    <  2:728582/111‑1 (MQ=255)
                                                                                                                                      ggATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGc                                                    >  1:728582/1‑111 (MQ=255)
                                                                                                                                      ggATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtt              >  2:426312/1‑149 (MQ=255)
                                                                                                                                        aTGCGGCGTTGACCCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTa                                                      >  4:338124/1‑107 (MQ=255)
                                                                                                                                        aTGCGGCGTTGACCCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTa                                                      <  3:338124/107‑1 (MQ=255)
                                                                                                                                        aTGCGGCGTTGACCCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGa                                            >  3:318644/1‑117 (MQ=255)
                                                                                                                                         tGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttt            <  3:655839/148‑1 (MQ=255)
                                                                                                                                          gCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTGTCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACttttt           <  3:277984/148‑1 (MQ=255)
                                                                                                                                           cGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttttt          <  4:824328/148‑1 (MQ=255)
                                                                                                                                           cGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttttt          <  4:1054861/148‑1 (MQ=255)
                                                                                                                                                tCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGt     <  3:597120/148‑1 (MQ=255)
                                                                                                                                                 cAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGtt    >  4:428065/1‑148 (MQ=255)
                                                                                                                                                  aaCGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTa  >  1:961958/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TTCGCTGCTGGAGCTTTTTCTGGCTGAGTAAACTTGTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTTTTACTGTTTA  >  NC_000913/3098373‑3098667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: