Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,256,441 T→G 8.2% T71P (ACG→CCG)  yhaM ← putative L‑serine dehydratase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,256,4410TG 8.2% 312.9 / 10.9 93T71P (ACG→CCG) yhaMputative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand):  ref base T (48/37);  new base G (4/4);  total (52/41)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGG  >  NC_000913/3256302‑3256585
                                                                                                                                           |                                                                                                                                                
gagaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGaa                                                                                                                                          >  4:547226/1‑148 (MQ=255)
 agaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACg                                                                                                                                        <  2:718183/149‑1 (MQ=255)
 agaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACg                                                                                                                                        >  2:631960/1‑149 (MQ=255)
 agaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATCGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAAc                                                                                                                                         >  3:492915/1‑148 (MQ=255)
  gaCTCTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACg                                                                                                                                        >  4:902737/1‑148 (MQ=255)
    cTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGt                                                                                                                                      <  3:192843/148‑1 (MQ=255)
    cTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGt                                                                                                                                      <  4:508077/148‑1 (MQ=255)
     tttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGc                                                                                                                                                <  1:337927/137‑1 (MQ=255)
     tttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGc                                                                                                                                                >  2:337927/1‑137 (MQ=255)
     tttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGa                                                                                                                                    >  1:961824/1‑149 (MQ=255)
      ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGa                                                                                                                                    >  3:456199/1‑148 (MQ=255)
       tCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGAc                                                                                                                                   <  3:547226/148‑1 (MQ=255)
         cccGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGcc                                                                                                                                <  1:822261/149‑1 (MQ=255)
          ccgccgCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGccc                                                                                                                               >  2:720036/1‑149 (MQ=255)
          ccgccgCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGcc                                                                                                                                <  4:289253/148‑1 (MQ=255)
                      gTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttcttc                                                                                                                   <  1:236890/149‑1 (MQ=255)
                      gTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttcttc                                                                                                                   >  2:27885/1‑149 (MQ=255)
                       tGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttcttc                                                                                                                   >  3:448/1‑148 (MQ=255)
                         cTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtcat                                                                                                                 <  4:561457/148‑1 (MQ=255)
                          tttGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtcatca                                                                                                               <  2:459263/149‑1 (MQ=255)
                           ttGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCACCCCGGCGTTGGCATTTCCCCCTAACGCCCCCAGCGCCGCCGCAATCGGCAACCCCACCATTCCCCGGCCGGGACCGGTGCCGCCCAGACCGTTCTtcatca                                                                                                               >  3:345550/1‑148 (MQ=255)
                                aTCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTg                                                                                                         <  2:961824/149‑1 (MQ=255)
                                 tCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTgg                                                                                                                                    >  4:1006828/1‑120 (MQ=255)
                                 tCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTgg                                                                                                                                    <  3:1006828/121‑2 (MQ=255)
                                  cGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTgg                                                                                                        >  3:932427/1‑148 (MQ=255)
                                       aTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGaaa                                                                                                   >  4:5360/1‑148 (MQ=255)
                                       aTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGaaa                                                                                                   >  3:845194/1‑148 (MQ=255)
                                       aTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAAc                                                                                                  <  2:980614/149‑1 (MQ=255)
                                           ccTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAg                                                                                              <  2:242475/149‑1 (MQ=255)
                                            cTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAg                                                                                              <  3:804826/148‑1 (MQ=255)
                                                 cTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTc                                                                                         >  3:167387/1‑148 (MQ=255)
                                                   gTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                                                                      <  2:131495/149‑1 (MQ=255)
                                                         tCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttctt                                                                                                                    <  4:862952/113‑1 (MQ=255)
                                                         tCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttctt                                                                                                                    >  3:862952/1‑113 (MQ=255)
                                                         tCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTc                                                                                >  2:904699/1‑149 (MQ=255)
                                                           tttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTACCGCCCCCAGCGCCGCCGCAATCGGCAGCCCCTTCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCGTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCa                                                                               <  4:89299/148‑1 (MQ=255)
                                                            ttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCaa                                                                              >  4:764903/1‑148 (MQ=255)
                                                            ttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCaa                                                                              >  4:542067/1‑148 (MQ=255)
                                                               aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCg                                                                           <  3:189280/148‑1 (MQ=255)
                                                                 cACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGAc                                                                        >  1:407618/1‑149 (MQ=255)
                                                                    ttCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTggggc                                                                                                                                 >  2:230202/1‑85 (MQ=37)
                                                                    ttCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTggggc                                                                                                                                 <  1:230202/89‑5 (MQ=37)
                                                                    ttCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCtt                                                                     >  2:930976/1‑149 (MQ=255)
                                                                    ttCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCt                                                                      <  4:448/148‑1 (MQ=255)
                                                                     tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCGGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCtt                                                                     >  3:648816/1‑148 (MQ=255)
                                                                     tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCtt                                                                     <  4:932427/148‑1 (MQ=255)
                                                                     tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTc                                                                    >  1:1063159/1‑149 (MQ=255)
                                                                      ccAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGa                                                                         >  1:690281/1‑143 (MQ=255)
                                                                      ccAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTCCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGa                                                                         <  2:690281/143‑1 (MQ=255)
                                                                       cAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc                                                                   >  4:407198/1‑148 (MQ=255)
                                                                       cAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc                                                                   <  3:746857/148‑1 (MQ=255)
                                                                       cAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCACCCCCACCATTCCCGTGCCGGGATCGATGACGCCCAGACCGTTCGTCATCAGAATTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTGCa                                                                  <  2:534391/149‑1 (MQ=255)
                                                                        aGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCa                                                                  >  4:1052970/1‑148 (MQ=255)
                                                                         gCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGt                                                                <  2:214195/149‑1 (MQ=255)
                                                                         gCCCGGCGTTGGCAATTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAg                                                                 >  3:690963/1‑148 (MQ=255)
                                                                            cGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCt                                                             <  2:155920/149‑1 (MQ=255)
                                                                             ggCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTg                                                            >  1:871184/1‑149 (MQ=255)
                                                                              gCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGc                                                           <  2:285283/149‑1 (MQ=255)
                                                                               cGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGc                                                           <  3:407198/148‑1 (MQ=255)
                                                                                  tGGCATTTCCACCTAACGCCCCCAGCGGCGGCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGGTTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgc                                                        >  4:648792/1‑148 (MQ=255)
                                                                                  tGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgca                                                       <  1:9497/149‑1 (MQ=255)
                                                                                  tGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgc                                                        >  3:295752/1‑148 (MQ=255)
                                                                                   ggCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGtt                                                                                 >  2:1030329/1‑122 (MQ=255)
                                                                                   ggCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGtt                                                                                 <  1:1030329/122‑1 (MQ=255)
                                                                                   ggCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgca                                                       <  4:492915/148‑1 (MQ=255)
                                                                                    gCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcagc                                                     >  2:3038/1‑149 (MQ=255)
                                                                                       tttCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAc                                                  >  1:86753/1‑149 (MQ=255)
                                                                                             ccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCtt                                                                     >  3:1010170/1‑124 (MQ=255)
                                                                                             ccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCtt                                                                     <  4:1010170/124‑1 (MQ=255)
                                                                                             ccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccg                                             >  3:842701/1‑148 (MQ=255)
                                                                                               tAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCCTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgcc                                           >  4:380602/1‑148 (MQ=255)
                                                                                                 aCGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACcgccgccgc                                         >  4:546284/1‑148 (MQ=255)
                                                                                                  cGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCgccgccgcc                                        >  3:104015/1‑148 (MQ=255)
                                                                                                       ccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgcc                                  >  1:742332/1‑149 (MQ=255)
                                                                                                        cAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACg                                                                                   <  4:432116/99‑1 (MQ=255)
                                                                                                        cAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACg                                                                                   >  3:432116/1‑99 (MQ=255)
                                                                                                         aGCGCCGCCACAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgcca                                 >  4:540726/1‑148 (MQ=255)
                                                                                                          gcgcCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgccag                                <  4:456199/148‑1 (MQ=255)
                                                                                                                 cgcAATCGGCAGCCCCACCATTCACGTGACGGGAACGGTGACGCCCAGAGCGTTCTTCATCAGATTTGGCGAAACTCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCTCCGCCGCCAGCGCCAGTGAAATc                         <  3:663285/148‑1 (MQ=255)
                                                                                                                   cAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCgg                       >  4:33459/1‑148 (MQ=255)
                                                                                                                    aaTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGt                      <  4:800717/148‑1 (MQ=255)
                                                                                                                     aTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTc                    <  1:27885/149‑1 (MQ=255)
                                                                                                                       cGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAg                  <  1:930976/149‑1 (MQ=255)
                                                                                                                           ggcgtcaccgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTAc               >  3:8322/6‑148 (MQ=255)
                                                                                                                            gCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACa              >  3:9523/1‑148 (MQ=255)
                                                                                                                             ccccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATc            >  2:997211/1‑149 (MQ=255)
                                                                                                                              cccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcc           >  2:302973/1‑149 (MQ=255)
                                                                                                                               ccaccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCACCAGTGAAATCGGTTCAGTACATccc          >  1:730299/1‑149 (MQ=255)
                                                                                                                                caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATccc          >  4:227959/1‑148 (MQ=255)
                                                                                                                                 accaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCa         >  3:104488/1‑148 (MQ=255)
                                                                                                                                     ttCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGcc    >  2:577970/1‑149 (MQ=255)
                                                                                                                                        ccGTGCCGGGAACGGTTACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCgg  <  3:902737/148‑1 (MQ=255)
                                                                                                                                        ccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCTGCCAGCGCCAGTGAAACCGGTTCAGTACATCCCAGcggcgg  <  4:958016/148‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                
GAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGG  >  NC_000913/3256302‑3256585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: