Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,328,182 A→T 7.7% intergenic (+99/+57) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,328,1820AT 7.7% 352.9 / 13.5 104intergenic (+99/+57)yhbY/greARNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
Reads supporting (aligned to +/- strand):  ref base A (43/52);  new base T (4/4);  total (47/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 106 total aligned reads displayed.

GGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATC  >  NC_000913/3328035‑3328326
                                                                                                                                                   |                                                                                                                                                 
ggTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGGGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCAt                                                                                                                                                  >  1:744845/1‑149 (MQ=255)
ggTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCa                                                                                                                                                   >  3:117603/1‑148 (MQ=255)
ggTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCa                                                                                                                                                   >  4:1031816/1‑148 (MQ=255)
 gTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATa                                                                                                                                                 >  2:47592/1‑149 (MQ=255)
    cTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCg                                                                                                                                             >  1:767768/1‑149 (MQ=255)
     tttATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCgg                                                                                                                                            >  1:129909/1‑149 (MQ=255)
         tCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTATGCGGCCt                                                                                                                                         <  4:509394/148‑1 (MQ=255)
         tCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCtt                                                                                                                                        >  1:570008/1‑149 (MQ=255)
         tCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCtt                                                                                                                                        <  2:83869/149‑1 (MQ=255)
           gCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCtttt                                                                                                                                      <  1:148648/149‑1 (MQ=255)
            cccAACTAAAGAACGTAAAATCTCGCTGCCACGCTCAGATCATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCtttt                                                                                                                                      <  4:689571/148‑1 (MQ=255)
              cAACTAAAGAACGTAAAATCTTGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCGGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTc                                                                                                                                    <  4:117603/148‑1 (MQ=255)
              cAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTcc                                                                                                                                   >  2:269471/1‑149 (MQ=255)
              cAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTcc                                                                                                                                   <  2:696608/149‑1 (MQ=255)
                aCTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTATGCGGCCttttatcaa                                                                                                                                 <  2:591093/149‑6 (MQ=255)
                aCTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctt                                                                                                                                 <  2:548571/149‑1 (MQ=255)
                aCTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCct                                                                                                                                  >  3:132984/1‑148 (MQ=255)
                  tAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttc                                                                                                                               <  1:269471/149‑1 (MQ=255)
                    aaGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttctt                                                                                                                             >  1:316836/1‑149 (MQ=255)
                    aaGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttctt                                                                                                                             >  2:843123/1‑149 (MQ=255)
                    aaGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttct                                                                                                                              <  4:364547/148‑1 (MQ=255)
                       aaCGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTAc                                                                                                                          <  2:461501/149‑1 (MQ=255)
                       aaCGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTAc                                                                                                                          <  1:47592/149‑1 (MQ=255)
                       aaCGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTACTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTa                                                                                                                           >  3:273391/1‑148 (MQ=255)
                           tAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATa                                                                                                                      >  1:499422/1‑149 (MQ=255)
                            aaaaTCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATa                                                                                                                      >  3:991317/1‑148 (MQ=255)
                            aaaaTCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATAc                                                                                                                     <  2:129909/149‑1 (MQ=255)
                             aaaTCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCttt                                                                                                                                       >  3:263118/1‑130 (MQ=255)
                                tctcGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATc                                                                                                                  <  4:782888/148‑1 (MQ=255)
                                   cGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACa                                                                                                              >  1:77016/1‑149 (MQ=255)
                                    gCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACAt                                                                                                             >  1:562589/1‑149 (MQ=255)
                                      tGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCt                                                                                                           <  2:938944/149‑1 (MQ=255)
                                       gCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCtt                                                                                                          <  1:321332/149‑1 (MQ=255)
                                       gCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCt                                                                                                           <  4:991317/148‑1 (MQ=255)
                                       gCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCt                                                                                                           <  4:511478/148‑1 (MQ=255)
                                         cACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGa                                                                                                        <  1:487720/149‑1 (MQ=255)
                                         cACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTATGCGGCCttttat                                                                                                                                    <  4:483305/121‑3 (MQ=255)
                                             cTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTa                                                                                                     >  3:920717/1‑148 (MQ=255)
                                                aGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTgg                                                                                                 <  2:639447/149‑1 (MQ=255)
                                                aGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTg                                                                                                  <  4:635349/148‑1 (MQ=255)
                                                     aTCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTaa                                                                                             <  4:132984/148‑1 (MQ=255)
                                                         tAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCtt                                                                                        >  1:97499/1‑149 (MQ=255)
                                                         tAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCtt                                                                                        >  2:929833/1‑149 (MQ=255)
                                                         tAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCt                                                                                         >  4:949597/1‑148 (MQ=255)
                                                          aaaGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCt                                                                                                           <  3:761178/129‑1 (MQ=255)
                                                          aaaGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCt                                                                                                           >  4:761178/1‑129 (MQ=255)
                                                          aaaGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTa                                                                                       <  2:228569/149‑1 (MQ=255)
                                                           aaGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTa                                                                                       <  4:10361/148‑1 (MQ=255)
                                                            aGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTAc                                                                                      <  3:855976/148‑1 (MQ=255)
                                                             gTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACa                                                                                     <  3:949597/148‑1 (MQ=255)
                                                                 cacacaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACa                                                                                     >  1:315404/1‑144 (MQ=255)
                                                                 cacacaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACa                                                                                     <  2:315404/144‑1 (MQ=255)
                                                                   cacaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTAtt                                                                               >  3:744397/1‑148 (MQ=255)
                                                                   cacaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTAtt                                                                               >  3:114622/1‑148 (MQ=255)
                                                                     caTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGGGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCa                                                                            <  2:744845/149‑1 (MQ=255)
                                                                     caTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTcc                                                                             >  4:922227/1‑148 (MQ=255)
                                                                     caTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTcc                                                                             >  4:664373/1‑148 (MQ=255)
                                                                     caTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTcc                                                                             >  3:211117/1‑148 (MQ=255)
                                                                      aTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCAc                                                                           >  1:830481/1‑149 (MQ=255)
                                                                             gtgtAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCCCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAAt                                                                     >  3:1007727/1‑148 (MQ=255)
                                                                               gtAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTa                                                                   <  3:253110/148‑1 (MQ=255)
                                                                                tAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTa                                                                                 <  1:4276/133‑1 (MQ=255)
                                                                                tAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTa                                                                                 >  2:4276/1‑133 (MQ=255)
                                                                                  aaaCGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCttttt                                                                                                                                     <  3:498066/79‑1 (MQ=255)
                                                                                  aaaCGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCttttt                                                                                                                                     >  4:498066/1‑79 (MQ=255)
                                                                                    aCGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCAGGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGAAATTCTTACAGGTATTCCACCTTAATTACTTCaa                                                             >  1:880630/1‑149 (MQ=255)
                                                                                       aGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCaaa                                                            <  2:779280/147‑1 (MQ=255)
                                                                                       aGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCaaa                                                            >  1:779280/1‑147 (MQ=255)
                                                                                         gggTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCt                                                        >  2:918947/1‑149 (MQ=255)
                                                                                            ttttCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACt                                                     <  2:499422/149‑1 (MQ=255)
                                                                                                        aggAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCgg                                          <  3:42222/148‑1 (MQ=255)
                                                                                                           agagCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGt                                       <  3:664373/148‑1 (MQ=255)
                                                                                                            gagCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGttt                                     <  2:880630/149‑1 (MQ=255)
                                                                                                            gagCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGttt                                     <  2:570008/149‑1 (MQ=255)
                                                                                                              gCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGtttt                                    <  4:211117/148‑1 (MQ=255)
                                                                                                               cAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtga                                  <  1:302460/149‑1 (MQ=255)
                                                                                                                aaaaTGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtgat                                 <  2:562589/149‑1 (MQ=255)
                                                                                                                aaaaTGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtga                                  <  3:1051442/148‑1 (MQ=255)
                                                                                                                 aaaTGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtgat                                 >  4:44318/1‑148 (MQ=255)
                                                                                                                              tGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAAcatcat                   >  2:146179/1‑149 (MQ=255)
                                                                                                                                ttCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtct                 <  2:77016/149‑1 (MQ=255)
                                                                                                                                  cGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttc               <  1:558940/149‑1 (MQ=255)
                                                                                                                                   gTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACCTCTTGAGTATTAGGTAATTCTTACAGGTATTCCCCCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttct              >  1:843655/1‑149 (MQ=255)
                                                                                                                                   gTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttc               <  3:996758/148‑1 (MQ=255)
                                                                                                                                    ttGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCt                                                        >  3:527640/1‑106 (MQ=255)
                                                                                                                                    ttGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCt                                                        <  4:527640/106‑1 (MQ=255)
                                                                                                                                    ttGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttctt             >  2:301659/1‑149 (MQ=255)
                                                                                                                                       aTAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttcttct           >  4:266584/1‑148 (MQ=255)
                                                                                                                                        aaaaaGGCCGCTATGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtgat                                 <  2:937428/124‑1 (MQ=255)
                                                                                                                                        aaaaaGGCCGCTATGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtgat                                 >  1:937428/2‑125 (MQ=255)
                                                                                                                                          aaaGGCCGCTATGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCCCCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttctt             >  1:16668/1‑143 (MQ=255)
                                                                                                                                          aaaGGCCGCTATGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttctt             <  2:16668/143‑1 (MQ=255)
                                                                                                                                           aaGGCCGCATAGCGGCCTTTTTCCTTTCTTTAGAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtga                                  <  1:1058136/121‑1 (MQ=255)
                                                                                                                                           aaGGCCGCATAGCGGCCTTTTTCCTTTCTTTAGAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtga                                  >  2:1058136/1‑121 (MQ=255)
                                                                                                                                           aaGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGc       <  3:242426/148‑1 (MQ=255)
                                                                                                                                            aGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtctt                <  4:1016690/138‑1 (MQ=255)
                                                                                                                                            aGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtctt                >  3:1016690/1‑138 (MQ=255)
                                                                                                                                              gCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCTACCTTAATTACTTCAAATTCTACTGCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGa    >  3:872460/1‑148 (MQ=255)
                                                                                                                                              gCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGAt   <  1:671985/149‑1 (MQ=255)
                                                                                                                                               ccGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATc  <  2:325951/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATC  >  NC_000913/3328035‑3328326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: