Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,331,979 T→C 8.2% T253A (ACG→GCG)  yhbE ← EamA family inner membrane putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,331,9790TC 8.2% 341.0 / 16.2 110T253A (ACG→GCG) yhbEEamA family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base T (56/45);  new base C (4/5);  total (60/50)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 119 total aligned reads displayed.

CGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCAGAGGG  >  NC_000913/3331831‑3332127
                                                                                                                                                    |                                                                                                                                                    
cGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGt                                                                                                                                                      <  2:619758/149‑1 (MQ=255)
 gATGACCAATTGCGGAATACATCGCGCGCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGtg                                                                                                                                                     >  2:471956/1‑149 (MQ=255)
  aTGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGtga                                                                                                                                                    <  1:442988/149‑1 (MQ=255)
  aTGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGtga                                                                                                                                                    <  1:249156/149‑1 (MQ=255)
   tGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGtgat                                                                                                                                                   <  2:888851/149‑1 (MQ=255)
   tGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGtga                                                                                                                                                    <  4:488942/148‑1 (MQ=255)
        aaTGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATc                                                                                                                                               >  4:558554/1‑148 (MQ=255)
              ggAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCa                                                                                                                                        >  1:174852/1‑149 (MQ=255)
              ggAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGCGATCATCACGCTc                                                                                                                                         <  3:439669/148‑1 (MQ=255)
              ggAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGCGATCATCACGCTCa                                                                                                                                        <  1:883469/149‑1 (MQ=255)
               gAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGCGATCATCACGCTCa                                                                                                                                        <  4:811084/148‑1 (MQ=255)
                 aTACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCAcc                                                                                                                                      <  3:9335/148‑1 (MQ=255)
                     aTCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTgcgc                                                                                                                                 >  2:789290/1‑149 (MQ=255)
                     aTCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTgcgc                                                                                                                                 >  1:569554/1‑149 (MQ=255)
                        ccgcCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGctg                                                                                                                               <  3:403823/147‑1 (MQ=255)
                          gcCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGctgcct                                                                                                                            <  1:408530/149‑1 (MQ=255)
                          gcCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGctgcct                                                                                                                            <  1:202083/149‑1 (MQ=255)
                              gCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCa                                                                                                                        >  2:103909/1‑149 (MQ=255)
                                aaCCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGc                                                                                                                      >  2:387136/1‑149 (MQ=255)
                                    aCGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGat                                                                                                                   >  3:505630/1‑147 (MQ=255)
                                    aCGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGc                                                                                                                  >  1:110348/1‑149 (MQ=255)
                                        cAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATc                                                                                                              <  2:1064551/149‑1 (MQ=255)
                                          aaCGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCg                                                                                                             >  4:771179/1‑148 (MQ=255)
                                          aaCGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGc                                                                                                            <  1:78448/149‑1 (MQ=255)
                                           aCGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCt                                                                                                           >  1:338228/1‑149 (MQ=255)
                                             gCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGCGATCATCACGCt                                                                                                                                          >  1:143686/1‑116 (MQ=255)
                                             gCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGCGATCATCACGCt                                                                                                                                          <  2:26418/116‑1 (MQ=255)
                                             gCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGCGATCATCACGCt                                                                                                                                          <  2:143686/116‑1 (MQ=255)
                                               aCCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCg                                                                                                       >  1:839129/1‑149 (MQ=255)
                                                         ccTAAACGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGgcgc                                                                                              >  3:186094/1‑148 (MQ=255)
                                                         ccTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATcgc                                                                                                                                             >  2:902012/1‑101 (MQ=255)
                                                         ccTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATcgc                                                                                                                                             <  1:902012/101‑1 (MQ=255)
                                                          cTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCa                                                                                            >  2:602637/1‑149 (MQ=255)
                                                          cTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCa                                                                                            >  1:879160/1‑149 (MQ=255)
                                                            aaaaGGTTTAACGTCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCat                                                                                           >  4:685442/1‑148 (MQ=255)
                                                            aaaaGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCat                                                                                           >  3:970701/1‑148 (MQ=255)
                                                            aaaaGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCat                                                                                           >  4:322435/1‑148 (MQ=255)
                                                             aaaGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCata                                                                                          >  3:169985/1‑148 (MQ=255)
                                                               aGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATc                                                                                        <  3:135544/148‑1 (MQ=255)
                                                                 gTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCt                                                                                                                                     <  2:500217/101‑1 (MQ=255)
                                                                 gTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCt                                                                                                                                     >  1:500217/1‑101 (MQ=255)
                                                                   ttAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTAc                                                                                    >  3:426488/1‑148 (MQ=255)
                                                                      aCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCaa                                                                                 >  4:161586/1‑148 (MQ=255)
                                                                      aCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCaa                                                                                 <  3:399012/148‑1 (MQ=255)
                                                                      aCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAg                                                                                <  1:136064/149‑1 (MQ=255)
                                                                      aCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAg                                                                                <  1:228288/149‑1 (MQ=255)
                                                                      aCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCAAATCCTACCaa                                                                                 <  3:685442/148‑1 (MQ=255)
                                                                      aCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCCGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTGCCAAg                                                                                >  1:1052797/1‑149 (MQ=255)
                                                                        aTCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGt                                                                              >  2:37587/1‑149 (MQ=255)
                                                                         tCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCatat                                                                                         >  3:798369/1‑137 (MQ=255)
                                                                         tCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCatat                                                                                         <  4:798369/137‑1 (MQ=255)
                                                                          cGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTAt                                                                            <  1:1007345/149‑1 (MQ=255)
                                                                          cGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGACAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTa                                                                             >  3:680547/1‑148 (MQ=255)
                                                                            gTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTc                                                                          >  1:725143/1‑149 (MQ=255)
                                                                             tCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCt                                                                                      >  4:22442/1‑136 (MQ=255)
                                                                             tCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCt                                                                                      <  3:22442/136‑1 (MQ=255)
                                                                                ggCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTc                                                                      <  2:551074/149‑1 (MQ=255)
                                                                                 gCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTcc                                                                     >  1:52329/1‑149 (MQ=255)
                                                                                    aagaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGc                                                                   >  3:829205/1‑148 (MQ=255)
                                                                                    aagaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGc                                                                   >  4:263710/1‑148 (MQ=255)
                                                                                    aagaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGc                                                                   >  3:219900/1‑148 (MQ=255)
                                                                                     agaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCt                                                                                      <  4:334164/128‑1 (MQ=255)
                                                                                       aaaTCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaaaa                                                               >  1:272109/1‑149 (MQ=255)
                                                                                         aTCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaaaaaa                                                             <  2:999449/149‑1 (MQ=255)
                                                                                         aTCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaaaaaa                                                             >  2:598720/1‑149 (MQ=255)
                                                                                           cGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAAtt                                                           <  1:659871/149‑1 (MQ=255)
                                                                                                            aaaaGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGcc                                           >  3:835845/1‑148 (MQ=255)
                                                                                                            aaaaGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGcc                                           >  3:632033/1‑148 (MQ=255)
                                                                                                             aaaGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAg                                         >  2:190530/1‑149 (MQ=255)
                                                                                                             aaaGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAg                                         >  1:527149/1‑149 (MQ=255)
                                                                                                               aGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGt                                        <  3:677777/148‑1 (MQ=255)
                                                                                                               aGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTg                                       <  1:387136/149‑1 (MQ=255)
                                                                                                                gATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTg                                       >  3:257886/1‑148 (MQ=255)
                                                                                                                    tGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTaa                                  >  2:74594/1‑149 (MQ=255)
                                                                                                                    tGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTaa                                  <  1:617136/149‑1 (MQ=255)
                                                                                                                    tGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTaa                                  >  2:602095/1‑149 (MQ=255)
                                                                                                                     gAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAg                                 <  2:52329/149‑1 (MQ=255)
                                                                                                                       aaaaaaCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCt                               >  1:214480/1‑149 (MQ=255)
                                                                                                                        aaaaaCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCt                               >  4:1048830/1‑148 (MQ=255)
                                                                                                                          aaaCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTgcg                            >  1:99248/1‑149 (MQ=255)
                                                                                                                               gCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTAt                        <  3:1048830/148‑1 (MQ=255)
                                                                                                                               gCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTAt                        <  3:177882/148‑1 (MQ=255)
                                                                                                                                cGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCa                      <  2:697687/149‑1 (MQ=255)
                                                                                                                                  tAAACAGTGGTGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGgc                                                                                                <  2:1006433/73‑1 (MQ=255)
                                                                                                                                  tAAACAGTGGTGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGgc                                                                                                >  1:1006433/1‑73 (MQ=255)
                                                                                                                                   aaaCAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACt                    >  4:741959/1‑148 (MQ=255)
                                                                                                                                   aaaCAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTc                   <  2:533261/149‑1 (MQ=255)
                                                                                                                                    aaCAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTcc                  <  2:110348/149‑1 (MQ=255)
                                                                                                                                    aaCAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTcc                  <  1:403647/149‑1 (MQ=255)
                                                                                                                                     aCAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCGGCCAGTGGCTAAGCTGCGCTATCACTcc                  <  4:446573/148‑1 (MQ=255)
                                                                                                                                        gTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAgg               >  3:122526/1‑148 (MQ=255)
                                                                                                                                           gggTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCtt            <  3:263710/148‑1 (MQ=255)
                                                                                                                                           aggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCttt           >  2:301210/2‑149 (MQ=255)
                                                                                                                                           aggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCttt           >  2:757733/2‑149 (MQ=255)
                                                                                                                                             gTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGc         <  1:643589/149‑1 (MQ=255)
                                                                                                                                               gAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGcc        <  4:169985/148‑1 (MQ=255)
                                                                                                                                               gAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCAAGGCTTTGcc        <  3:602044/148‑1 (MQ=255)
                                                                                                                                                 gCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATCTCCTACCAAGGTATTCAGTGCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCag      <  3:651390/148‑1 (MQ=255)
                                                                                                                                                  cGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCagag    <  1:74594/149‑1 (MQ=255)
                                                                                                                                                    tgatgaTCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCAGAggg  <  2:214480/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGATGACCAATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCAGAGGG  >  NC_000913/3331831‑3332127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: