Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,331,983 G→C 8.2% I251M (ATC→ATG yhbE ← EamA family inner membrane putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,331,9830GC 8.2% 377.7 / 20.4 110I251M (ATC→ATGyhbEEamA family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base G (56/45);  new base C (4/5);  total (60/50)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 116 total aligned reads displayed.

AATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCAGAGGGAAGA  >  NC_000913/3331839‑3332131
                                                                                                                                                |                                                                                                                                                    
aaTGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATc                                                                                                                                                   >  4:558554/1‑148 (MQ=255)
      ggAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGCGATCATCACGCTc                                                                                                                                             <  3:439669/148‑1 (MQ=255)
      ggAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGCGATCATCACGCTCa                                                                                                                                            <  1:883469/149‑1 (MQ=255)
         aTACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCAcc                                                                                                                                          <  3:9335/148‑1 (MQ=255)
             aTCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTgcgc                                                                                                                                     >  2:789290/1‑149 (MQ=255)
                ccgcCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGctg                                                                                                                                   <  3:403823/147‑1 (MQ=255)
                 cgcCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGctgcc                                                                                                                                 <  1:168212/149‑1 (MQ=255)
                  gcCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGctgcct                                                                                                                                <  1:202083/149‑1 (MQ=255)
                  gcCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGctgcct                                                                                                                                <  1:408530/149‑1 (MQ=255)
                      gCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCa                                                                                                                            >  2:103909/1‑149 (MQ=255)
                            aCGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGat                                                                                                                       >  3:505630/1‑147 (MQ=255)
                            aCGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGc                                                                                                                      >  1:110348/1‑149 (MQ=255)
                                cAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATc                                                                                                                  <  2:1064551/149‑1 (MQ=255)
                                  aaCGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCg                                                                                                                 >  4:771179/1‑148 (MQ=255)
                                   aCGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCt                                                                                                               >  1:338228/1‑149 (MQ=255)
                                     gCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGCGATCATCACGCt                                                                                                                                              >  1:26418/1‑116 (MQ=255)
                                     gCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGCGATCATCACGCt                                                                                                                                              <  2:143686/116‑1 (MQ=255)
                                     gCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGCGATCATCACGCt                                                                                                                                              >  1:143686/1‑116 (MQ=255)
                                     gCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGCGATCATCACGCt                                                                                                                                              <  2:26418/116‑1 (MQ=255)
                                       aCCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCg                                                                                                           >  1:839129/1‑149 (MQ=255)
                                       aCCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCg                                                                                                           >  2:73085/1‑149 (MQ=255)
                                                 ccTAAACGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGgcgc                                                                                                  >  3:186094/1‑148 (MQ=255)
                                                 ccTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATcgc                                                                                                                                                 <  1:902012/101‑1 (MQ=255)
                                                  cTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCa                                                                                                >  2:602637/1‑149 (MQ=255)
                                                  cTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCa                                                                                                >  1:879160/1‑149 (MQ=255)
                                                    aaaaGGTTTAACGTCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCat                                                                                               >  4:685442/1‑148 (MQ=255)
                                                    aaaaGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCat                                                                                               >  3:970701/1‑148 (MQ=255)
                                                    aaaaGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCat                                                                                               >  4:322435/1‑148 (MQ=255)
                                                     aaaGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCatat                                                                                             >  1:626202/1‑149 (MQ=255)
                                                     aaaGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCata                                                                                              >  3:169985/1‑148 (MQ=255)
                                                      aaGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATc                                                                                            <  2:174852/149‑1 (MQ=255)
                                                       aGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATc                                                                                            <  3:135544/148‑1 (MQ=255)
                                                         gTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCt                                                                                                                                         <  2:500217/101‑1 (MQ=255)
                                                         gTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCt                                                                                                                                         >  1:500217/1‑101 (MQ=255)
                                                          tttAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTa                                                                                         >  4:602044/1‑148 (MQ=255)
                                                           ttAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTAc                                                                                        >  3:426488/1‑148 (MQ=255)
                                                           ttAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTAc                                                                                        >  4:298684/1‑148 (MQ=255)
                                                             aaCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCaa                                                                                     <  1:440144/149‑1 (MQ=255)
                                                              aCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCaa                                                                                     <  3:399012/148‑1 (MQ=255)
                                                              aCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCaa                                                                                     >  4:161586/1‑148 (MQ=255)
                                                              aCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAg                                                                                    <  1:228288/149‑1 (MQ=255)
                                                              aCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAg                                                                                    <  1:136064/149‑1 (MQ=255)
                                                              aCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCAAATCCTACCaa                                                                                     <  3:685442/148‑1 (MQ=255)
                                                              aCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCCGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTGCCAAg                                                                                    >  1:1052797/1‑149 (MQ=255)
                                                                aTCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGt                                                                                  >  2:37587/1‑149 (MQ=255)
                                                                 tCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCatat                                                                                             >  3:798369/1‑137 (MQ=255)
                                                                 tCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCatat                                                                                             <  4:798369/137‑1 (MQ=255)
                                                                  cGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTAt                                                                                <  1:1007345/149‑1 (MQ=255)
                                                                  cGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGACAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTa                                                                                 >  3:680547/1‑148 (MQ=255)
                                                                    gTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTc                                                                              >  1:725143/1‑149 (MQ=255)
                                                                     tCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCt                                                                                          >  4:22442/1‑136 (MQ=255)
                                                                     tCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCt                                                                                          <  3:22442/136‑1 (MQ=255)
                                                                      cTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCa                                                                             <  3:683061/148‑1 (MQ=255)
                                                                        ggCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTc                                                                          <  2:551074/149‑1 (MQ=255)
                                                                         gCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTcc                                                                         >  1:52329/1‑149 (MQ=255)
                                                                         gCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTc                                                                          <  3:52474/148‑1 (MQ=255)
                                                                            aagaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGc                                                                       >  4:263710/1‑148 (MQ=255)
                                                                            aagaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGc                                                                       >  3:219900/1‑148 (MQ=255)
                                                                             agaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCt                                                                                          <  4:334164/128‑1 (MQ=255)
                                                                               aaaTCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaaaa                                                                   >  1:272109/1‑149 (MQ=255)
                                                                                 aTCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCaaaaaa                                                                 <  2:999449/149‑1 (MQ=255)
                                                                                   cGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAAtt                                                               <  1:659871/149‑1 (MQ=255)
                                                                                   cGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAAtt                                                               >  2:466455/1‑149 (MQ=255)
                                                                                      gccaggccagTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTaaa                                                            <  2:5961/149‑1 (MQ=255)
                                                                                                    aaaaGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGcc                                               >  3:835845/1‑148 (MQ=255)
                                                                                                    aaaaGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGcc                                               >  3:632033/1‑148 (MQ=255)
                                                                                                     aaaGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAg                                             >  1:527149/1‑149 (MQ=255)
                                                                                                       aGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGt                                            <  3:677777/148‑1 (MQ=255)
                                                                                                        gATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTg                                           >  3:257886/1‑148 (MQ=255)
                                                                                                           cTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCt                                        >  3:185382/1‑148 (MQ=255)
                                                                                                            tGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTaa                                      >  2:602095/1‑149 (MQ=255)
                                                                                                            tGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTaa                                      <  1:617136/149‑1 (MQ=255)
                                                                                                            tGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTaa                                      >  2:74594/1‑149 (MQ=255)
                                                                                                             gAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAg                                     <  2:52329/149‑1 (MQ=255)
                                                                                                               aaaaaaCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCt                                   >  1:214480/1‑149 (MQ=255)
                                                                                                                aaaaaCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCt                                   >  4:1048830/1‑148 (MQ=255)
                                                                                                                  aaaCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTgcg                                >  1:99248/1‑149 (MQ=255)
                                                                                                                       gCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTAt                            <  3:1048830/148‑1 (MQ=255)
                                                                                                                        cGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCa                          <  2:697687/149‑1 (MQ=255)
                                                                                                                          tAAACAGTGGTGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGgc                                                                                                    >  1:1006433/1‑73 (MQ=255)
                                                                                                                          tAAACAGTGGTGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGgc                                                                                                    <  2:1006433/73‑1 (MQ=255)
                                                                                                                           aaaCAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACt                        >  4:741959/1‑148 (MQ=255)
                                                                                                                           aaaCAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTc                       <  2:533261/149‑1 (MQ=255)
                                                                                                                            aaCAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTcc                      <  2:110348/149‑1 (MQ=255)
                                                                                                                            aaCAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTcc                      <  1:403647/149‑1 (MQ=255)
                                                                                                                             aCAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCa                     >  2:955993/1‑149 (MQ=255)
                                                                                                                                gTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAgg                   >  3:122526/1‑148 (MQ=255)
                                                                                                                                   gggTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCtt                <  3:263710/148‑1 (MQ=255)
                                                                                                                                   aggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCttt               >  2:757733/2‑149 (MQ=255)
                                                                                                                                   aggTGAGCGCGATCATCACGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCttt               >  2:301210/2‑149 (MQ=255)
                                                                                                                                     gTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGc             <  1:643589/149‑1 (MQ=255)
                                                                                                                                         gCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATCTCCTACCAAGGTATTCAGTGCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCag          <  3:651390/148‑1 (MQ=255)
                                                                                                                                          cGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCagag        <  1:789290/149‑1 (MQ=255)
                                                                                                                                          cGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCagag        <  1:74594/149‑1 (MQ=255)
                                                                                                                                            tgatgaTCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCAGAggg      <  2:214480/149‑1 (MQ=255)
                                                                                                                                              atgatCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCAGAGGGaa    <  2:338228/149‑1 (MQ=255)
                                                                                                                                              atgatCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCAGAGGGa     >  4:111929/1‑148 (MQ=255)
                                                                                                                                               tgatCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCAGAGGGAag   >  2:384683/1‑149 (MQ=255)
                                                                                                                                                gatCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCACGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCAGAGGGAag   <  4:186094/148‑1 (MQ=255)
                                                                                                                                                gatCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCAGAGGGAaga  <  1:955993/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
AATGGCGGAATACATCGCGCCCGCAACCACGACAAACGCACCGAGATAACCTAAAAGGTTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGCGCCATATCCTACCAAGGTATTCAGTCCGCAAAAAATTAAACATGCGAGCTGCCAGTGGCTAAGCTGCGCTATCACTCCAGGCTTTGCCAGAGGGAAGA  >  NC_000913/3331839‑3332131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: