Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,333,050 T→A 5.6% K24* (AAG→TAG)  rpmA ← 50S ribosomal subunit protein L27

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,333,0500TA 5.6% 375.3 / 10.2 107K24* (AAG→TAG) rpmA50S ribosomal subunit protein L27
Reads supporting (aligned to +/- strand):  ref base T (51/50);  new base A (3/3);  total (54/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

Only 100 of 107 total aligned reads displayed.

TTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCCTGCT  >  NC_000913/3332905‑3333195
                                                                                                                                                 |                                                                                                                                                 
ttAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTaa                                                                                                                                                 >  4:755762/1‑148 (MQ=255)
ttAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTaa                                                                                                                                                 <  4:408276/148‑1 (MQ=255)
ttAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGACGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTaa                                                                                                                                                 <  3:623187/148‑1 (MQ=255)
   aCTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTAAACGc                                                                                                                                              >  4:55014/1‑148 (MQ=255)
     ttCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCa                                                                                                                                           >  2:596701/1‑149 (MQ=255)
      tCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCa                                                                                                                                           >  3:309039/1‑148 (MQ=255)
           tttCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGtt                                                                                                                                     >  2:430288/1‑149 (MQ=255)
           tttCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGt                                                                                                                                      >  3:551709/1‑148 (MQ=255)
           tttCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGAATCGCCACCGAAACGCTTAACGCCCAGGCGtt                                                                                                                                     >  2:1033311/1‑149 (MQ=255)
              cACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTa                                                                                                                                   <  4:46183/148‑1 (MQ=255)
               aCTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTAAACGCCTGGGCGTTAAg                                                                                                                                  <  3:55014/148‑1 (MQ=255)
                  ttACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCtt                                                                                                                               <  4:551709/148‑1 (MQ=255)
                     ccGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTg                                                                                                                            <  3:755762/148‑1 (MQ=255)
                            cTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATcgcg                                                                                                                     <  4:89798/148‑1 (MQ=255)
                            cTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATcgcg                                                                                                                     <  4:783658/148‑1 (MQ=255)
                            cTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATc                                                                                                                        <  1:1069453/145‑1 (MQ=255)
                            cTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATc                                                                                                                        >  2:1069453/1‑145 (MQ=255)
                             tttAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGa                                                                                                                    <  3:638168/148‑1 (MQ=255)
                             tttAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTAGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGAc                                                                                                                   <  1:789230/149‑1 (MQ=255)
                                 gCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCCCGGTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGtt                                                                                                               <  1:972657/149‑1 (MQ=255)
                                   aaaCAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTAc                                                                                                             >  1:854383/1‑149 (MQ=255)
                                    aaCAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTAAACGCCTGGGCGttaa                                                                                                                                   >  4:486719/1‑124 (MQ=255)
                                    aaCAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGATACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTAc                                                                                                             >  3:317636/1‑148 (MQ=255)
                                     aCAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACgt                                                                                                           <  2:683882/149‑1 (MQ=255)
                                     aCAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACg                                                                                                            >  3:739456/1‑148 (MQ=255)
                                      cAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACgtg                                                                                                          <  1:979328/149‑1 (MQ=255)
                                       agagTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACgtg                                                                                                          <  3:61135/148‑1 (MQ=255)
                                        gagTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACgtgt                                                                                                         <  4:495630/148‑1 (MQ=255)
                                               gTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAgccgc                                                                                                 >  2:416994/1‑149 (MQ=255)
                                                tCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAgccgc                                                                                                 >  3:774442/1‑148 (MQ=255)
                                                  aCGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCTGGGCGTTAAGcgttt                                                                                                                             >  4:169721/1‑114 (MQ=255)
                                                  aCGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCTGGGCGTTAAGcgttt                                                                                                                             <  3:169721/118‑5 (MQ=255)
                                                  aCGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCTGGGCGTTAAGcgttt                                                                                                                             <  4:307273/118‑5 (MQ=255)
                                                  aCGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCTGGGCGTTAAGcgttt                                                                                                                             >  3:307273/1‑114 (MQ=255)
                                                  aCGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAg                                                                                              >  1:325859/1‑149 (MQ=255)
                                                   cGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAgg                                                                                                                                         >  4:796325/1‑105 (MQ=255)
                                                   cGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAgg                                                                                                                                         <  3:796325/105‑1 (MQ=255)
                                                     aCCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGcc                                                                                            >  4:661075/1‑148 (MQ=255)
                                                        gCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCttt                                                                                         >  3:1004035/1‑148 (MQ=255)
                                                           aCCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTAt                                                                                     >  2:522080/1‑149 (MQ=255)
                                                             cTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTAtgt                                                                                   <  2:505898/149‑1 (MQ=255)
                                                             cTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTAtgt                                                                                   <  2:401358/149‑1 (MQ=255)
                                                                   tAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCAtt                                                                             >  2:603879/1‑149 (MQ=255)
                                                                        ccAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGaaa                                                                        <  1:416994/149‑1 (MQ=255)
                                                                        ccAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGaa                                                                         <  4:393670/148‑1 (MQ=255)
                                                                            cGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCTAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAAtct                                                                     <  4:108210/148‑1 (MQ=255)
                                                                               ggAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAAtctctc                                                                  <  4:739456/148‑1 (MQ=255)
                                                                               ggAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAAtctct                                                                   >  3:924887/1‑147 (MQ=255)
                                                                               ggAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAAtctct                                                                   <  4:924887/147‑1 (MQ=255)
                                                                                gAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTAt                                                                                     >  1:919902/1‑128 (MQ=255)
                                                                                gAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTctcc                                                                 >  4:95537/1‑148 (MQ=255)
                                                                                gAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGAGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTAt                                                                                     <  2:919902/128‑1 (MQ=255)
                                                                                  aTTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCa                                                              <  1:982679/149‑1 (MQ=255)
                                                                                   tttGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAg                                                             >  2:697227/1‑149 (MQ=255)
                                                                                        tACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTAt                                                                                     <  1:745110/120‑1 (MQ=255)
                                                                                         aCCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTa                                                       <  1:430288/149‑1 (MQ=255)
                                                                                           cACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGt                                                                                                                >  3:115346/1‑90 (MQ=255)
                                                                                           cACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGt                                                                                                                <  4:115346/90‑1 (MQ=255)
                                                                                                tGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCt                                                 >  3:713542/1‑148 (MQ=255)
                                                                                                 gACGAACGATGATGCTACCCTCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTGAGGCGCTGa                                               >  1:77594/1‑149 (MQ=255)
                                                                                                 gACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGa                                               <  1:600435/149‑1 (MQ=255)
                                                                                                  acgaacgaTGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGAt                                              <  1:508558/149‑1 (MQ=255)
                                                                                                    gaacgaTGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGc                                            <  1:20661/149‑1 (MQ=255)
                                                                                                      acgaTGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGcc                                           <  3:38323/148‑1 (MQ=255)
                                                                                                      acgaTGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGcc                                           >  3:205643/1‑148 (MQ=255)
                                                                                                      acgaTGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCa                                          <  1:1030966/149‑1 (MQ=255)
                                                                                                      acgaTGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCa                                          <  2:854383/149‑1 (MQ=255)
                                                                                                        gatgatGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGt                                        >  1:755211/1‑149 (MQ=255)
                                                                                                             tgCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGcc                                           >  4:944465/1‑141 (MQ=255)
                                                                                                              gCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAAtttt                                  <  1:981406/149‑1 (MQ=255)
                                                                                                                tACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTca                                >  1:436795/1‑149 (MQ=255)
                                                                                                                tACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTca                                >  1:789964/1‑149 (MQ=255)
                                                                                                                 aCCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCa                                                              >  3:367420/1‑118 (MQ=255)
                                                                                                                 aCCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCa                                                              <  4:367420/118‑1 (MQ=255)
                                                                                                                 aCCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTcac                               >  2:857649/1‑149 (MQ=255)
                                                                                                                  cccGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTcaca                              >  1:1065935/1‑149 (MQ=255)
                                                                                                                     gCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCa                           <  1:1033311/149‑1 (MQ=255)
                                                                                                                        aGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTg                        <  1:522080/149‑1 (MQ=255)
                                                                                                                        aGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTg                        >  2:461699/1‑149 (MQ=255)
                                                                                                                        aGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTg                        <  2:789964/149‑1 (MQ=255)
                                                                                                                           aCGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAAc                     <  1:697227/149‑1 (MQ=255)
                                                                                                                              gATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCAc                  >  1:310202/1‑149 (MQ=255)
                                                                                                                                  cGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGAc              <  2:989426/149‑1 (MQ=255)
                                                                                                                                   gCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCATTAATTTTCACATCAGTGAACCACTGACg             >  2:641225/1‑149 (MQ=255)
                                                                                                                                   gCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACg             >  1:188076/1‑149 (MQ=255)
                                                                                                                                    ccACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACg             >  4:424468/1‑148 (MQ=255)
                                                                                                                                    ccACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACg             >  4:210876/1‑148 (MQ=255)
                                                                                                                                     cACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGAt           >  1:319886/1‑149 (MQ=255)
                                                                                                                                      aCCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGAt           >  4:608023/1‑148 (MQ=255)
                                                                                                                                        cGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATgg         <  4:309039/148‑1 (MQ=255)
                                                                                                                                          aaaCGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACgt            <  3:55556/143‑2 (MQ=255)
                                                                                                                                          aaaCGCTTAACGCCCAGGCGTTTAGCTTCTGAATAGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACgt            >  4:55556/1‑142 (MQ=255)
                                                                                                                                           aaCGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGaa                                                                         <  3:678157/81‑1 (MQ=255)
                                                                                                                                           aaCGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGaa                                                                         >  4:678157/1‑81 (MQ=255)
                                                                                                                                           aaCGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCct     >  2:709009/1‑149 (MQ=255)
                                                                                                                                           aaCGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCct     >  1:556327/1‑149 (MQ=255)
                                                                                                                                            aagCTAAACGCCTGGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGaa                                                                         >  3:1008806/3‑80 (MQ=255)
                                                                                                                                            aagCTAAACGCCTGGGCGTCTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGaa                                                                         <  4:1008806/78‑1 (MQ=255)
                                                                                                                                             cGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCctgc   <  1:820853/149‑1 (MQ=255)
                                                                                                                                               cTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTGACGTGTGGAGCCGCCAACCTTTGTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTGACATCAGTGAACCACTGACGATCGCCctgct  >  3:655089/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCCTGCT  >  NC_000913/3332905‑3333195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: