Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,333,058 A→G 8.2% L21P (CTG→CCG) ‡ rpmA ← 50S ribosomal subunit protein L27

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,333,0580AG 8.2% 332.0 / 16.5 110L21P (CTG→CCG) ‡rpmA50S ribosomal subunit protein L27
Reads supporting (aligned to +/- strand):  ref base A (48/53);  new base G (4/5);  total (52/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.81e-02

Only 100 of 113 total aligned reads displayed.

TTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCCTGCTGCTTACGATAG  >  NC_000913/3332910‑3333206
                                                                                                                                                    |                                                                                                                                                    
ttCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCa                                                                                                                                                      >  2:596701/1‑149 (MQ=255)
 tCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCa                                                                                                                                                      >  3:309039/1‑148 (MQ=255)
      tttCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGtt                                                                                                                                                >  2:430288/1‑149 (MQ=255)
          aCTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTAAACGCCTGGGCGTTAAg                                                                                                                                             <  3:55014/148‑1 (MQ=255)
             ttACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCtt                                                                                                                                          <  4:551709/148‑1 (MQ=255)
                ccGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTg                                                                                                                                       <  3:755762/148‑1 (MQ=255)
                       cTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATcgcg                                                                                                                                <  4:783658/148‑1 (MQ=255)
                       cTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATcgcg                                                                                                                                <  4:89798/148‑1 (MQ=255)
                       cTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATc                                                                                                                                   <  1:1069453/145‑1 (MQ=255)
                       cTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATc                                                                                                                                   >  2:1069453/1‑145 (MQ=255)
                        tttAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGa                                                                                                                               <  4:545948/148‑1 (MQ=255)
                        tttAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGa                                                                                                                               <  3:638168/148‑1 (MQ=255)
                        tttAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTAGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGAc                                                                                                                              <  1:789230/149‑1 (MQ=255)
                            gCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCCCGGTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGtt                                                                                                                          <  1:972657/149‑1 (MQ=255)
                              aaaCAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTAc                                                                                                                        >  1:854383/1‑149 (MQ=255)
                               aaCAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTAAACGCCTGGGCGttaa                                                                                                                                              <  3:486719/126‑3 (MQ=255)
                               aaCAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTAAACGCCTGGGCGttaa                                                                                                                                              >  4:486719/1‑124 (MQ=255)
                               aaCAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGATACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTAc                                                                                                                        >  3:317636/1‑148 (MQ=255)
                                aCAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACgt                                                                                                                      <  2:683882/149‑1 (MQ=255)
                                aCAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACg                                                                                                                       >  3:739456/1‑148 (MQ=255)
                                 cAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACgtg                                                                                                                     <  1:979328/149‑1 (MQ=255)
                                  agagTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACgtg                                                                                                                     <  3:61135/148‑1 (MQ=255)
                                   gagTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACgtgt                                                                                                                    <  4:495630/148‑1 (MQ=255)
                                          gTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAgccgc                                                                                                            >  2:416994/1‑149 (MQ=255)
                                             aCGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCTGGGCGTTAAGcgttt                                                                                                                                        <  3:169721/118‑5 (MQ=255)
                                             aCGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCTGGGCGTTAAGcgttt                                                                                                                                        >  4:169721/1‑114 (MQ=255)
                                             aCGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCTGGGCGTTAAGcgttt                                                                                                                                        >  3:307273/1‑114 (MQ=255)
                                             aCGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCTGGGCGTTAAGcgttt                                                                                                                                        <  4:307273/118‑5 (MQ=255)
                                             aCGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAg                                                                                                         >  1:325859/1‑149 (MQ=255)
                                              cGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAgg                                                                                                                                                    <  3:796325/105‑1 (MQ=255)
                                              cGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAgg                                                                                                                                                    >  4:796325/1‑105 (MQ=255)
                                                aCCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGcc                                                                                                       >  4:661075/1‑148 (MQ=255)
                                                   gCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCttt                                                                                                    >  3:1004035/1‑148 (MQ=255)
                                                        cTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTAtgt                                                                                              <  2:401358/149‑1 (MQ=255)
                                                        cTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTAtgt                                                                                              <  2:505898/149‑1 (MQ=255)
                                                              tAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCAtt                                                                                        >  2:603879/1‑149 (MQ=255)
                                                                   ccAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGaaa                                                                                   <  1:416994/149‑1 (MQ=255)
                                                                   ccAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGaa                                                                                    <  4:393670/148‑1 (MQ=255)
                                                                       cGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCTAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAAtct                                                                                <  4:108210/148‑1 (MQ=255)
                                                                          ggAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAAtctct                                                                              >  3:924887/1‑147 (MQ=255)
                                                                          ggAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAAtctct                                                                              <  4:924887/147‑1 (MQ=255)
                                                                           gAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTAt                                                                                                >  1:919902/1‑128 (MQ=255)
                                                                           gAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTctcc                                                                            >  4:95537/1‑148 (MQ=255)
                                                                           gAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGAGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTAt                                                                                                <  2:919902/128‑1 (MQ=255)
                                                                             aTTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCa                                                                         <  1:982679/149‑1 (MQ=255)
                                                                              tttGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAg                                                                        >  2:697227/1‑149 (MQ=255)
                                                                                   tACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTAt                                                                                                >  2:745110/1‑120 (MQ=255)
                                                                                   tACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTAt                                                                                                <  1:745110/120‑1 (MQ=255)
                                                                                    aCCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTa                                                                  <  1:430288/149‑1 (MQ=255)
                                                                                      cACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGt                                                                                                                           >  3:115346/1‑90 (MQ=255)
                                                                                           tGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCt                                                            >  3:713542/1‑148 (MQ=255)
                                                                                            gACGAACGATGATGCTACCCTCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTGAGGCGCTGa                                                          >  1:77594/1‑149 (MQ=255)
                                                                                            gACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGa                                                          <  1:600435/149‑1 (MQ=255)
                                                                                             acgaacgaTGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGAt                                                         <  1:508558/149‑1 (MQ=255)
                                                                                               gaacgaTGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGc                                                       <  1:20661/149‑1 (MQ=255)
                                                                                                 acgaTGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGcc                                                      <  3:38323/148‑1 (MQ=255)
                                                                                                 acgaTGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCa                                                     <  1:1030966/149‑1 (MQ=255)
                                                                                                   gatgatGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGt                                                   >  1:755211/1‑149 (MQ=255)
                                                                                                      gatgCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAAt                                                >  1:989426/1‑149 (MQ=255)
                                                                                                        tgCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGcc                                                      >  4:944465/1‑141 (MQ=255)
                                                                                                        tgCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGcc                                                      <  3:944465/141‑1 (MQ=255)
                                                                                                         gCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAAtttt                                             <  1:981406/149‑1 (MQ=255)
                                                                                                           tACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTca                                           >  1:436795/1‑149 (MQ=255)
                                                                                                           tACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTca                                           >  1:789964/1‑149 (MQ=255)
                                                                                                            aCCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCa                                                                         <  4:367420/118‑1 (MQ=255)
                                                                                                            aCCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCa                                                                         >  3:367420/1‑118 (MQ=255)
                                                                                                            aCCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTcac                                          >  2:857649/1‑149 (MQ=255)
                                                                                                             cccGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTcaca                                         >  1:1065935/1‑149 (MQ=255)
                                                                                                                gCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCa                                      <  1:1033311/149‑1 (MQ=255)
                                                                                                                   aGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTg                                   >  2:461699/1‑149 (MQ=255)
                                                                                                                   aGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTg                                   <  1:522080/149‑1 (MQ=255)
                                                                                                                   aGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTg                                   <  2:789964/149‑1 (MQ=255)
                                                                                                                      aCGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAAc                                <  1:697227/149‑1 (MQ=255)
                                                                                                                         gATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCAc                             >  1:310202/1‑149 (MQ=255)
                                                                                                                             cGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGAc                         <  2:989426/149‑1 (MQ=255)
                                                                                                                              gCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCATTAATTTTCACATCAGTGAACCACTGACg                        >  2:641225/1‑149 (MQ=255)
                                                                                                                              gCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACg                        >  1:188076/1‑149 (MQ=255)
                                                                                                                               ccACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACg                        >  4:424468/1‑148 (MQ=255)
                                                                                                                               ccACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACg                        >  4:210876/1‑148 (MQ=255)
                                                                                                                                 aCCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGAt                      >  4:608023/1‑148 (MQ=255)
                                                                                                                                   cGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATgg                    <  4:309039/148‑1 (MQ=255)
                                                                                                                                     aaaCGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACgt                       <  3:55556/143‑2 (MQ=255)
                                                                                                                                     aaaCGCTTAACGCCCAGGCGTTTAGCTTCTGAATAGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACgt                       >  4:55556/1‑142 (MQ=255)
                                                                                                                                      aaCGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGaa                                                                                    >  4:678157/1‑81 (MQ=255)
                                                                                                                                      aaCGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGaa                                                                                    <  3:678157/81‑1 (MQ=255)
                                                                                                                                      aaCGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCct                >  2:709009/1‑149 (MQ=255)
                                                                                                                                      aaCGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCct                >  1:556327/1‑149 (MQ=255)
                                                                                                                                       aagCTAAACGCCTGGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGaa                                                                                    >  3:1008806/3‑80 (MQ=255)
                                                                                                                                       aagCTAAACGCCTGGGCGTCTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGaa                                                                                    <  4:1008806/78‑1 (MQ=255)
                                                                                                                                          cTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTGACGTGTGGAGCCGCCAACCTTTGTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTGACATCAGTGAACCACTGACGATCGCCctgct             >  3:655089/1‑148 (MQ=255)
                                                                                                                                             aaCGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCCTgctgct          <  4:1004035/148‑1 (MQ=255)
                                                                                                                                                 cccAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGaaa                                                                                   >  3:881162/1‑71 (MQ=255)
                                                                                                                                                 cccAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGaaa                                                                                   <  4:881162/71‑1 (MQ=255)
                                                                                                                                                 cccAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCCTGCTGCTTACGa     <  1:461699/149‑1 (MQ=255)
                                                                                                                                                  ccAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAgg                                                                       >  1:232256/1‑82 (MQ=255)
                                                                                                                                                  ccAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAgg                                                                       <  2:232256/82‑1 (MQ=255)
                                                                                                                                                   cAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCCTGCTGCTTACGAt    <  4:317636/148‑1 (MQ=255)
                                                                                                                                                   cAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCCTGCTGCTTACGAt    <  3:248567/148‑1 (MQ=255)
                                                                                                                                                   cAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCCTGCTGCTTACGATa   <  1:596701/149‑1 (MQ=255)
                                                                                                                                                    aGGCGTTTAGCTTCTGAATCGCGACCGGTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCCTGCTGCTTACGATAg  >  2:108637/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTTAGCGCCAGCGTGGAATTTGGTACCACGTTGACGAACGATGATGCTACCCGCCAGAACGGATTCGCCACCGAAACGCTTAACGCCCAGGCGTTTAGCTTCTGAATCGCGACCGTTACGTGTGGAGCCGCCAGCCTTTTTATGTGCCATTTGAAATCTCTCCTCAGGTCTTAGGCGCTGATGCCAGTAATTTTCACATCAGTGAACCACTGACGATGGCCCTGCTGCTTACGATAG  >  NC_000913/3332910‑3333206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: