Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,360,643 T→A 11.0% intergenic (+27/‑533) gltD → / → gltF glutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,360,6430TA11.0% 229.2 / 17.7 73intergenic (+27/‑533)gltD/gltFglutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (31/34);  new base A (4/4);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

GACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAATT  >  NC_000913/3360496‑3360790
                                                                                                                                                   |                                                                                                                                                   
gACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACt                                                                                                                                                     >  4:75584/1‑148 (MQ=255)
 aCCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACtgt                                                                                                                                                   <  2:685859/149‑1 (MQ=255)
 aCCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACtg                                                                                                                                                    <  4:634314/148‑1 (MQ=255)
           cGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGa                                                                                                                                          >  3:126775/1‑148 (MQ=255)
            gAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACACAGTCTGGCGaa                                                                                                                                         <  4:658816/148‑1 (MQ=255)
                 tCTTTGCTGGCGGTGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAAttcat                                                                                                                                    <  4:710152/148‑5 (MQ=255)
                 tCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGGATTCGCCAGACACAGTCTGGCGAAttcatt                                                                                                                                   <  2:684106/149‑6 (MQ=255)
                 tCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCaaa                                                                                                                                    >  3:1002760/1‑148 (MQ=255)
                 tCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCACACGGTATTATGAGCTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCaaa                                                                                                                                    <  3:98123/148‑1 (MQ=255)
                   tttGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAAtt                                                                                                                                  <  4:126775/148‑1 (MQ=255)
                   tttGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTa                                                                                                                                 >  2:868582/1‑149 (MQ=255)
                     tGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTAc                                                                                                                                <  4:1002760/148‑1 (MQ=255)
                      gCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACt                                                                                                                               <  3:607273/148‑1 (MQ=255)
                                      tCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCgttgt                                                                                                              >  2:225936/1‑149 (MQ=255)
                                       ccGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAACTTACTGACGAAGTGCGCgttgt                                                                                                              >  4:696840/1‑148 (MQ=255)
                                       ccGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACACAGTCTGGCGa                                                                                                                                          <  3:613651/120‑1 (MQ=255)
                                       ccGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGCCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACACAGTCTGGCGa                                                                                                                                          >  4:613651/1‑120 (MQ=255)
                                          tGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTc                                                                                                          <  1:392153/149‑1 (MQ=255)
                                           ggTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTc                                                                                                          <  4:391493/148‑1 (MQ=255)
                                            gTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTg                                                                                                        >  2:601663/1‑149 (MQ=255)
                                             ttCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACACAGTCTGGCGAAttca                                                                                                                                     >  3:1049296/1‑116 (MQ=255)
                                             ttCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACACAGTCTGGCGAAttca                                                                                                                                     <  4:1049296/119‑4 (MQ=255)
                                                 gATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCaaa                                                                                                   >  1:498572/1‑149 (MQ=255)
                                                 gATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACACAGTCTGGCg                                                                                                                                           >  1:905171/1‑109 (MQ=255)
                                                  aTCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAAGGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCaaa                                                                                                   >  3:169484/1‑148 (MQ=255)
                                                     tggtggTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCg                                                                                               <  1:260009/149‑1 (MQ=255)
                                                     tggtggTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCAGGCAAATGc                                                                                                <  3:67549/148‑1 (MQ=255)
                                                      ggtggtGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCg                                                                                               >  4:209372/1‑148 (MQ=255)
                                                        tggtgACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGc                                                                                             <  3:75584/148‑1 (MQ=255)
                                                         ggtgACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTc                                                                                                          >  4:75894/1‑134 (MQ=255)
                                                         ggtgACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTc                                                                                                          <  3:75894/134‑1 (MQ=255)
                                                           tgACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATg                                                                                          <  3:830973/148‑1 (MQ=255)
                                                           tgACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGa                                                                                         <  2:790862/149‑1 (MQ=255)
                                                            gACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAg                                                                                        >  1:812117/1‑149 (MQ=255)
                                                                gCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGt                                                                                     >  3:364102/1‑148 (MQ=255)
                                                                  tATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTg                                                                                  >  1:809777/1‑149 (MQ=255)
                                                                   aTTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTg                                                                                  >  4:211342/1‑148 (MQ=255)
                                                                    ttGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGAc                                                                                <  2:498572/149‑1 (MQ=255)
                                                                        cGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCttt                                                                            <  2:812117/149‑1 (MQ=255)
                                                                          aaGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCgt                                                                                                                 >  4:258029/1‑110 (MQ=255)
                                                                          aaGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCgt                                                                                                                 <  3:258029/110‑1 (MQ=255)
                                                                           aGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTcc                                                                          >  4:904088/1‑148 (MQ=255)
                                                                               cGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCtata                                                                      <  3:349965/148‑1 (MQ=255)
                                                                               cGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTataa                                                                     <  2:747634/149‑1 (MQ=255)
                                                                                  aaGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTATGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTataata                                                                   <  3:757253/148‑1 (MQ=255)
                                                                                   aGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAg                                                                  <  3:696840/148‑1 (MQ=255)
                                                                                          aGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAAtt                                                          >  1:850990/1‑149 (MQ=255)
                                                                                           gACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAAtt                                                          <  4:794034/148‑1 (MQ=255)
                                                                                            aCGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAAtttt                                                        >  1:364532/1‑149 (MQ=255)
                                                                                             cGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAAtttt                                                        <  3:371992/148‑1 (MQ=255)
                                                                                               gTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCaa                                                     >  1:216608/1‑149 (MQ=255)
                                                                                                   tatGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTcc                                                                          <  1:509858/124‑1 (MQ=255)
                                                                                                   tatGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTcc                                                                          >  2:509858/1‑124 (MQ=255)
                                                                                                     tGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAAtt                                               <  1:868582/149‑1 (MQ=255)
                                                                                                     tGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAAt                                                >  4:427648/1‑148 (MQ=255)
                                                                                                        aCTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATtgt                                             <  4:305050/148‑1 (MQ=255)
                                                                                                        aCTGGCAGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATtgt                                             >  4:105149/1‑148 (MQ=255)
                                                                                                             ctggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCttt                                        >  4:142869/1‑148 (MQ=255)
                                                                                                              tggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCgg                                                                                              >  2:134360/1‑93 (MQ=255)
                                                                                                              tggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCgg                                                                                              <  1:134360/93‑1 (MQ=255)
                                                                                                              tggAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGt                                      >  2:951995/1‑149 (MQ=255)
                                                                                                                gAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTc                                    >  1:482354/1‑149 (MQ=255)
                                                                                                                 aaGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTTCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTc                                    >  4:733528/1‑148 (MQ=255)
                                                                                                                                aaCAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAg                     <  3:427648/148‑1 (MQ=255)
                                                                                                                                   aaTGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGa                  <  4:169484/148‑1 (MQ=255)
                                                                                                                                   aaTGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGa                  <  3:209372/148‑1 (MQ=255)
                                                                                                                                    aTGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAg                >  2:358137/1‑149 (MQ=255)
                                                                                                                                        aTTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGcaca            >  2:887070/1‑149 (MQ=255)
                                                                                                                                        aTTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGcac             <  4:551504/148‑1 (MQ=255)
                                                                                                                                        aTTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGcac             <  3:249106/148‑1 (MQ=255)
                                                                                                                                         ttCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGcaca            >  3:443167/1‑148 (MQ=255)
                                                                                                                                         ttCGCCAGACACAGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACAt           >  2:752451/1‑149 (MQ=255)
                                                                                                                                          tCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATc          <  1:951995/149‑1 (MQ=255)
                                                                                                                                            gCCAGTCTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCaa        <  2:162767/149‑1 (MQ=255)
                                                                                                                                               aGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCa     >  1:402201/1‑149 (MQ=255)
                                                                                                                                                  cTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAAtt  <  2:482354/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAGTGAAGCACATCAAGCAATT  >  NC_000913/3360496‑3360790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: