Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,982 G→C 5.6% intergenic (+28/+32) yhcG → / ← yhcH DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily/DUF386 family protein, cupin superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,9820GC 5.6% 446.2 / 13.4 125intergenic (+28/+32)yhcG/yhcHDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily/DUF386 family protein, cupin superfamily
Reads supporting (aligned to +/- strand):  ref base G (62/56);  new base C (2/5);  total (64/61)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 129 total aligned reads displayed.

GGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCC  >  NC_000913/3368835‑3369128
                                                                                                                                                   |                                                                                                                                                  
ggTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAg                                                                                                                                                    >  3:964001/1‑148 (MQ=255)
 gTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAgtg                                                                                                                                                  >  2:392770/1‑149 (MQ=255)
  tttGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAgtg                                                                                                                                                  >  4:725575/1‑148 (MQ=255)
  tttGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAgtg                                                                                                                                                  >  4:249307/1‑148 (MQ=255)
    tGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCt                                                                                                                                               <  2:1010209/149‑1 (MQ=255)
        tAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCa                                                                                                                                            >  3:252013/1‑148 (MQ=255)
        tAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCa                                                                                                                                            >  3:645976/1‑148 (MQ=255)
        tAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAg                                                                                                                                           >  1:657521/1‑149 (MQ=255)
        tAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACACTGTCTGCa                                                                                                                                            <  3:786351/148‑1 (MQ=255)
         aaCACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAg                                                                                                                                           >  3:240468/1‑148 (MQ=255)
         aaCACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAg                                                                                                                                           >  3:532099/1‑148 (MQ=255)
         aaCACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAg                                                                                                                                           >  4:554008/1‑148 (MQ=255)
         aaCACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAg                                                                                                                                           >  3:901247/1‑148 (MQ=255)
          acacCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACACTGTCTGCAGaa                                                                                                                                         <  1:464787/149‑1 (MQ=255)
            acCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATc                                                                                                                                       <  1:352998/149‑1 (MQ=255)
             ccGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATc                                                                                                                                       >  3:152561/1‑148 (MQ=255)
             ccGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCt                                                                                                                                      <  2:250994/149‑1 (MQ=255)
                    ggCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTaaaa                                                                                                                                >  3:301827/1‑148 (MQ=255)
                       aaGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGa                                                                                                                             <  4:570960/148‑1 (MQ=255)
                        aGCGAATATAAGTTGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGaa                                                                                                                            >  3:466900/1‑148 (MQ=255)
                           gAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTa                                                                                                                         <  4:152561/148‑1 (MQ=255)
                           gAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTa                                                                                                                         <  4:901247/148‑1 (MQ=255)
                             atatAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAg                                                                                                                       >  3:388054/1‑148 (MQ=255)
                              tataAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGtt                                                                                                                     >  1:823380/1‑149 (MQ=255)
                                 aaGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTa                                                                                                                  >  1:501397/1‑149 (MQ=255)
                                  aGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTa                                                                                                                  <  4:388054/148‑1 (MQ=255)
                                  aGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTa                                                                                                                  <  3:554008/148‑1 (MQ=255)
                                   gATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTaa                                                                                                                 <  3:763501/148‑1 (MQ=255)
                                    aTGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGc                                                                                                               <  2:741207/149‑1 (MQ=255)
                                     tGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGc                                                                                                               >  4:470523/1‑148 (MQ=255)
                                                gatgaGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGc                                                                                                   >  2:348083/1‑149 (MQ=255)
                                                 atgaGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGcc                                                                                                  >  1:640485/1‑149 (MQ=255)
                                                 atgaGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGcc                                                                                                  >  1:961319/1‑149 (MQ=255)
                                                     gaAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTaa                                                                                              >  1:664335/1‑149 (MQ=255)
                                                     gaAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTaa                                                                                              <  2:546310/149‑1 (MQ=255)
                                                      aaaCGGCTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATGCTGTAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTGCAAGCCATTAAATCAGCCtgacc                                                                                             <  2:987732/149‑5 (MQ=255)
                                                      aaaCGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTaa                                                                                              <  3:947545/148‑1 (MQ=255)
                                                        aCGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAAcc                                                                                            >  4:110774/1‑148 (MQ=255)
                                                         cGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTa                                                                                                                  <  4:140035/125‑1 (MQ=255)
                                                         cGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTa                                                                                                                  >  3:140035/1‑125 (MQ=255)
                                                             tCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacc                                                                                      >  2:275839/1‑149 (MQ=255)
                                                             tCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacc                                                                                      >  2:836231/1‑149 (MQ=255)
                                                              cGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacca                                                                                     >  2:746093/1‑149 (MQ=255)
                                                              cGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacc                                                                                      >  4:678269/1‑148 (MQ=255)
                                                                 aGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACaa                                                                                  <  1:738566/149‑1 (MQ=255)
                                                                  gATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACaa                                                                                  <  4:645976/148‑1 (MQ=255)
                                                                   aTGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAAc                                                                                 >  4:91349/1‑148 (MQ=255)
                                                                     gAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCt                                                                               <  4:645252/148‑1 (MQ=255)
                                                                        cTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTaaaa                                                                                                                                >  2:544841/1‑96 (MQ=255)
                                                                         tCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCttttt                                                                           >  3:258929/1‑148 (MQ=255)
                                                                         tCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTa                                                                          >  1:328269/1‑149 (MQ=255)
                                                                          cGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCtt                                                                              >  4:248568/1‑144 (MQ=255)
                                                                          cGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTa                                                                          >  3:767184/1‑148 (MQ=255)
                                                                          cGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTa                                                                          >  3:879463/1‑148 (MQ=255)
                                                                           gTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTaa                                                                         <  3:678269/148‑1 (MQ=255)
                                                                           gTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACACTGTCTGCAGAAtt                                                                                                                                       <  2:449227/86‑2 (MQ=255)
                                                                            ttCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTg                                                                                                                                              >  1:710064/1‑78 (MQ=255)
                                                                            ttCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTg                                                                                                                                              <  2:710064/78‑1 (MQ=255)
                                                                            ttCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAtc                                                                       >  2:118455/1‑149 (MQ=255)
                                                                            ttCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAt                                                                        >  3:247293/1‑148 (MQ=255)
                                                                              cGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAtctc                                                                     <  1:1017131/149‑1 (MQ=255)
                                                                                 acacAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAtctctc                                                                   <  4:240468/148‑1 (MQ=255)
                                                                                  cacaGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCa                                                                 <  1:2264/149‑1 (MQ=255)
                                                                                  cacaGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAAGCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTcc                                                                  <  4:804464/148‑1 (MQ=255)
                                                                                   acaGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAg                                                                <  1:96861/149‑1 (MQ=255)
                                                                                     aGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAgg                                                               >  3:62622/1‑148 (MQ=255)
                                                                                      ggCGGTGCTCGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGc                                                              >  4:167821/1‑148 (MQ=255)
                                                                                         ggTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGc                                                          <  2:397615/149‑1 (MQ=255)
                                                                                               agagGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACa                                                     >  3:565049/1‑148 (MQ=255)
                                                                                                gagGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAAc                                                   >  1:455210/1‑149 (MQ=255)
                                                                                                    aaGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCa                                                >  4:45026/1‑148 (MQ=255)
                                                                                                        cTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTg                                           <  1:746093/149‑1 (MQ=255)
                                                                                                         tataGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTgg                                          >  1:890519/1‑149 (MQ=255)
                                                                                                          ataGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTaaa                                                                                                        <  4:657799/86‑1 (MQ=255)
                                                                                                          ataGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTaaa                                                                                                        >  3:657799/1‑86 (MQ=255)
                                                                                                             gACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGttt                                       >  3:9286/1‑148 (MQ=255)
                                                                                                             gACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGttt                                       >  3:261981/1‑148 (MQ=255)
                                                                                                               cGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTAt                                     >  4:332359/1‑148 (MQ=255)
                                                                                                                gCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTaa                                                                                                                 <  2:482750/71‑1 (MQ=255)
                                                                                                                gCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTaa                                                                                                                 >  1:482750/1‑71 (MQ=255)
                                                                                                                gCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATg                                    <  4:466900/148‑1 (MQ=255)
                                                                                                                gCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGc                                   >  1:163475/1‑149 (MQ=255)
                                                                                                                 ccGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGc                                   >  4:522828/1‑148 (MQ=255)
                                                                                                                  cGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCgg                                 <  2:351044/149‑1 (MQ=255)
                                                                                                                  cGTTAATTTCAGCGGTTTCGAATTATGCAGACAGTGTCTGCAGAATCTTTACAACGAAATAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCTCAACCTCTTTAATCACACCAGGCTCGCCTACAACGCATCCTGGTTTATGCgg                                 <  2:81165/149‑1 (MQ=255)
                                                                                                                    ttAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGtt                               >  1:909090/1‑149 (MQ=255)
                                                                                                                    ttAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGCTTATGCGGt                                >  3:23200/1‑148 (MQ=255)
                                                                                                                        tttCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCAc                            <  3:167821/148‑1 (MQ=255)
                                                                                                                         ttCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCAcc                           <  3:186272/148‑1 (MQ=255)
                                                                                                                         ttCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCt                          <  1:348083/149‑1 (MQ=255)
                                                                                                                         ttCAGCGGTTACGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCt                          <  2:40982/149‑1 (MQ=255)
                                                                                                                              cGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCa                      >  4:1024689/1‑148 (MQ=255)
                                                                                                                                  ttCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATaaa                  >  3:397623/1‑148 (MQ=255)
                                                                                                                                    aagaTTCTGCAGACACTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAacac               >  1:747268/4‑149 (MQ=255)
                                                                                                                                       aTTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGc            <  2:287208/149‑1 (MQ=255)
                                                                                                                                         tCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCg           >  4:156180/1‑148 (MQ=255)
                                                                                                                                              aGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACAtt     <  2:640485/149‑1 (MQ=255)
                                                                                                                                              aGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACAtt     <  2:961319/149‑1 (MQ=255)
                                                                                                                                               gACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTc    <  1:129087/149‑1 (MQ=255)
                                                                                                                                                 cAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATTAACACGGCGAACATTcc   <  4:301827/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCC  >  NC_000913/3368835‑3369128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: