Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,477,878 T→A 6.6% intergenic (+20/+29) slyX → / ← slyD phi X174 lysis protein/FKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,8780TA 6.6% 310.2 / 11.1 91intergenic (+20/+29)slyX/slyDphi X174 lysis protein/FKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base T (48/37);  new base A (3/3);  total (51/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.04e-01

GACCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCGTGGTGG  >  NC_000913/3477732‑3478024
                                                                                                                                                  |                                                                                                                                                  
gACCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAtt                                                                                                                                                   <  4:264349/148‑1 (MQ=255)
       cATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCtt                                                                                                                                           <  2:813026/149‑1 (MQ=255)
       cATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCt                                                                                                                                            <  3:32662/148‑1 (MQ=255)
       cATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTCAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCt                                                                                                                                            >  3:208545/1‑148 (MQ=255)
        aTGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCttt                                                                                                                                          >  2:783251/1‑149 (MQ=255)
        aTGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCttt                                                                                                                                          <  1:572406/149‑1 (MQ=255)
             aTGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTg                                                                                                                                      >  3:523079/1‑148 (MQ=255)
              tGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGt                                                                                                                                     >  3:185729/1‑148 (MQ=255)
                gagaTGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCa                                                                                                                                   >  3:440261/1‑148 (MQ=255)
                  gaTGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCATGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACtt                                                                                                                                >  2:951317/1‑149 (MQ=255)
                  gaTGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACtt                                                                                                                                <  1:790584/149‑1 (MQ=255)
                      gCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAGACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACttttt                                                                                                                             >  3:768956/1‑148 (MQ=255)
                      gCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTc                                                                                                                            >  2:72901/1‑149 (MQ=255)
                       cGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCg                                                                                                                           >  2:818871/1‑149 (MQ=255)
                        gAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCgg                                                                                                                          >  1:1065207/1‑149 (MQ=255)
                         aaaCTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCttttt                                                                                                                                        <  4:163801/134‑1 (MQ=255)
                         aaaCTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCttttt                                                                                                                                        >  3:163801/1‑134 (MQ=255)
                              gcgcGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAg                                                                                                                    <  2:138649/149‑1 (MQ=255)
                                gcgATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGt                                                                                                                   <  3:844857/148‑1 (MQ=255)
                                 cgATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCt                                                                                                                                            >  4:946321/1‑122 (MQ=255)
                                 cgATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCt                                                                                                                                            <  3:946321/122‑1 (MQ=255)
                                 cgATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTgg                                                                                                                 <  2:624588/149‑1 (MQ=255)
                                  gATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgc                                                                                                                >  1:279331/1‑149 (MQ=255)
                                      atcTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgcaac                                                                                                             <  3:611536/148‑1 (MQ=255)
                                       tcTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgcaaccg                                                                                                           >  1:1036050/1‑149 (MQ=255)
                                        cTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgcaaccg                                                                                                           >  3:48042/1‑148 (MQ=255)
                                                  tgACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGtt                                                                                                >  1:457652/1‑149 (MQ=255)
                                                    aCCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTg                                                                                               <  4:963216/148‑1 (MQ=255)
                                                    aCCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTg                                                                                               >  3:681249/1‑148 (MQ=255)
                                                    aCCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTg                                                                                               <  4:523079/148‑1 (MQ=255)
                                                     ccGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCtttt                                                                                                                                         >  4:937608/1‑105 (MQ=255)
                                                     ccGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCtttt                                                                                                                                         <  3:937608/105‑1 (MQ=255)
                                                      cGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCt                                                                                            <  1:783251/149‑1 (MQ=255)
                                                       gagaAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCt                                                                                            >  4:841483/1‑148 (MQ=255)
                                                        agaAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCtt                                                                                           >  4:585714/1‑148 (MQ=255)
                                                        agaAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCtt                                                                                           >  4:279896/1‑148 (MQ=255)
                                                         gaAGTTAAAAGCCAGCCAGCCGTGGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTATTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCttt                                                                                          >  3:662400/1‑148 (MQ=255)
                                                          aaGTTAAAAGCCAGCCAGCCTTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTAc                                                                                        <  2:457652/149‑1 (MQ=255)
                                                          aaGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTAc                                                                                        >  1:433053/1‑149 (MQ=255)
                                                           aGTCAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTAc                                                                                        <  4:208545/148‑1 (MQ=255)
                                                            gTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgcaa                                                                                                              <  1:425083/125‑1 (MQ=255)
                                                            gTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgcaa                                                                                                              >  2:425083/1‑125 (MQ=255)
                                                             ttAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCg                                                                                      <  3:841483/148‑1 (MQ=255)
                                                             ttAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGc                                                                                     >  2:1008804/1‑149 (MQ=255)
                                                              tAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGcc                                                                                    >  1:1009196/1‑149 (MQ=255)
                                                              tAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGc                                                                                     >  4:555787/1‑148 (MQ=255)
                                                                 aaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCcaca                                                                                 >  2:1011427/1‑149 (MQ=255)
                                                                  agccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCAcag                                                                                >  2:625113/1‑149 (MQ=255)
                                                                    ccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCAcagca                                                                              <  2:23432/149‑1 (MQ=255)
                                                                    ccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCAcagc                                                                               <  3:741528/148‑1 (MQ=255)
                                                                      agccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACagcagc                                                                            >  2:268220/1‑149 (MQ=255)
                                                                         cagccGTCGAACATCGCCTCGCAGGCTGAAGAAACGCCACCGCCACATTTTTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCt                                                                          <  4:662400/148‑1 (MQ=255)
                                                                           gccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTg                                                                                               <  3:770459/125‑1 (MQ=255)
                                                                           gccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTg                                                                                               >  4:770459/1‑125 (MQ=255)
                                                                               tCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCa                                                                    >  4:335133/1‑148 (MQ=255)
                                                                                cGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCAc                                                                   >  3:493757/1‑148 (MQ=255)
                                                                                  aaCATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCgt                                                                >  2:801877/1‑149 (MQ=255)
                                                                                      tCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGtt                                                             >  4:663590/1‑148 (MQ=255)
                                                                                       cgcgTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTc                                                            >  4:624560/1‑148 (MQ=255)
                                                                                          gTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATg                                                         <  3:852779/148‑1 (MQ=255)
                                                                                                 gCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGAt                                                 >  1:738593/1‑149 (MQ=255)
                                                                                                  cTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATc                                                <  1:818871/149‑1 (MQ=255)
                                                                                                   tGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCg                                               >  1:669958/1‑149 (MQ=255)
                                                                                                    gaagaaACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGt                                              <  2:1009196/149‑1 (MQ=255)
                                                                                                     aagaaACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTg                                             <  1:72901/149‑1 (MQ=255)
                                                                                                             ccaccgccacATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGa                                      <  3:279896/148‑1 (MQ=255)
                                                                                                              caccgccacATTATTAAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTAGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGAcc                                    >  1:5316/1‑149 (MQ=255)
                                                                                                               accgccacATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGAccg                                   <  1:1008804/149‑1 (MQ=255)
                                                                                                                ccgccacATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGAccg                                   >  4:636932/1‑148 (MQ=255)
                                                                                                                    cacaTTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACcgccgc                               <  4:1027547/148‑1 (MQ=255)
                                                                                                                    cacaTTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCgca                              >  2:394349/1‑149 (MQ=255)
                                                                                                                       attattGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCgcagc                            >  4:633429/1‑148 (MQ=255)
                                                                                                                         tattGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTACATGACCGTGATCGTAGCCATGACCGCCGCAGCaa                          <  4:768956/148‑1 (MQ=255)
                                                                                                                               ggCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACcgtcg                    >  4:747990/1‑148 (MQ=255)
                                                                                                                                gCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtg                  >  1:108286/1‑149 (MQ=255)
                                                                                                                                     aaaaaaGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcgt             >  1:330014/1‑149 (MQ=255)
                                                                                                                                      aaaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcgtg            >  1:524416/1‑149 (MQ=255)
                                                                                                                                         aaGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGAt          <  4:493757/148‑1 (MQ=255)
                                                                                                                                         aaGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATc         <  2:377689/149‑1 (MQ=255)
                                                                                                                                          aGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGt                                                                                                                   >  4:296059/1‑42 (MQ=255)
                                                                                                                                          aGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGt                                                                                                                   <  3:296059/42‑1 (MQ=255)
                                                                                                                                           gCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCg        >  3:98858/1‑148 (MQ=255)
                                                                                                                                            cGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgtg      <  1:424006/149‑1 (MQ=255)
                                                                                                                                            cGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgtg      <  1:341116/149‑1 (MQ=255)
                                                                                                                                            cGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgt       <  3:69550/148‑1 (MQ=255)
                                                                                                                                            cGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGGCGTGATCgt       >  3:279729/1‑148 (MQ=255)
                                                                                                                                              gggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgtgg     <  3:624560/148‑1 (MQ=255)
                                                                                                                                               ggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgtggtg   <  1:291963/149‑1 (MQ=255)
                                                                                                                                               ggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgtggt    >  3:22495/1‑148 (MQ=255)
                                                                                                                                                gATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCtt                                                                         <  3:110622/78‑1 (MQ=255)
                                                                                                                                                gATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCtt                                                                         >  4:110622/1‑78 (MQ=255)
                                                                                                                                                 aTTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgtggtgg  <  3:633429/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GACCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCGTGGTGG  >  NC_000913/3477732‑3478024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑