Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,480,662 T→A 5.7% H167L (CAC→CTC)  kefG ← potassium‑efflux system ancillary protein for KefB, glutathione‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,480,6620TA 5.7% 378.8 / 10.3 106H167L (CAC→CTC) kefGpotassium‑efflux system ancillary protein for KefB, glutathione‑regulated
Reads supporting (aligned to +/- strand):  ref base T (43/57);  new base A (2/4);  total (45/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 108 total aligned reads displayed.

CAGCGCCAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGC  >  NC_000913/3480514‑3480810
                                                                                                                                                    |                                                                                                                                                    
caGCGCCAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGt                                                                                                                                                      <  2:917683/149‑1 (MQ=255)
  gcgcCAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTgg                                                                                                                                                    <  1:1031232/149‑1 (MQ=255)
      cAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTc                                                                                                                                                 >  4:889870/1‑148 (MQ=255)
       aaTACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCg                                                                                                                                                >  4:702005/1‑148 (MQ=255)
       aaTACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCg                                                                                                                                                <  4:428943/148‑1 (MQ=255)
       aaTACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCg                                                                                                                                                >  3:538388/1‑148 (MQ=255)
           cccAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAga                                                                                                                                           <  2:424319/149‑2 (MQ=255)
            ccAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCCGCt                                                                                                                                          >  2:411862/1‑149 (MQ=255)
            ccAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAga                                                                                                                                           <  3:583778/148‑2 (MQ=255)
            ccAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAga                                                                                                                                           <  3:378067/148‑2 (MQ=255)
                 cgcgATTCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCtg                                                                                                                                      <  3:232568/148‑1 (MQ=255)
                         cAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCt                                                                                                                                          >  2:545208/1‑136 (MQ=255)
                         cAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCt                                                                                                                                          <  1:545208/136‑1 (MQ=255)
                           gCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGcc                                                                                                                           <  1:53664/149‑1 (MQ=255)
                                    cAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGcc                                                                                                                   <  4:448036/148‑1 (MQ=255)
                                     aGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGccc                                                                                                                  <  3:275085/148‑1 (MQ=255)
                                     aGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGccc                                                                                                                  <  3:1024375/148‑1 (MQ=255)
                                       ccACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGt                                                                                                               >  1:170856/1‑149 (MQ=255)
                                        cACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTa                                                                                                              <  1:209322/149‑1 (MQ=255)
                                             ccgcGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAaata                                                                                                          >  3:178752/1‑148 (MQ=255)
                                                 gAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCt                                                                                                                                                  <  1:638021/104‑1 (MQ=255)
                                                 gAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCt                                                                                                                                                  >  2:638021/1‑104 (MQ=255)
                                                 gAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgat                                                                                                     <  1:144222/149‑1 (MQ=255)
                                                 gAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatga                                                                                                      <  3:389587/148‑1 (MQ=255)
                                                  aaGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgatg                                                                                                    <  2:181817/149‑1 (MQ=255)
                                                  aaGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgatg                                                                                                    <  1:1028378/149‑1 (MQ=255)
                                                   agagAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgatg                                                                                                    <  3:755660/148‑1 (MQ=255)
                                                    gagaAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGTCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATgg                                                                                                   <  3:124483/148‑1 (MQ=255)
                                                     agaAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGa                                                                                                 <  1:221003/149‑1 (MQ=255)
                                                        aaCAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACtt                                                                                              >  2:610486/1‑149 (MQ=255)
                                                              aCTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                        <  1:950869/149‑1 (MQ=255)
                                                               cTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                        >  4:921535/1‑148 (MQ=255)
                                                               cTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                        <  4:272181/148‑1 (MQ=255)
                                                               cTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                        >  3:260641/1‑148 (MQ=255)
                                                               cTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGCCTCGCCCAGTAAATAATGATGGGACTTAACCAAt                                                                                       >  1:526464/1‑149 (MQ=255)
                                                               cTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGACACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                        <  3:739901/148‑1 (MQ=255)
                                                                tCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATg                                                                                      >  2:926359/1‑149 (MQ=255)
                                                                tCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATg                                                                                      >  2:389561/1‑149 (MQ=255)
                                                                    gCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTaa                                                                                            <  4:156212/139‑1 (MQ=255)
                                                                      gAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCg                                                                                >  1:782407/1‑149 (MQ=255)
                                                                        gTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCgg                                                                               <  3:678237/148‑1 (MQ=255)
                                                                           aaaaaTCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGcaca                                                                           >  2:114797/1‑149 (MQ=255)
                                                                           aaaaaTCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGcaca                                                                           >  2:776330/1‑149 (MQ=255)
                                                                           aaaaaTCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGcaca                                                                           <  1:389561/149‑1 (MQ=255)
                                                                                tCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgat                                                                                                     <  1:452621/118‑1 (MQ=255)
                                                                                   gAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGTACATGCccgcc                                                                   <  1:562399/149‑1 (MQ=255)
                                                                                    aaCCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCccgcc                                                                   >  4:202497/1‑148 (MQ=255)
                                                                                       cTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTgcgc                                                                                                                                 <  1:104757/83‑1 (MQ=255)
                                                                                       cTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTgcgc                                                                                                                                 >  2:104757/1‑83 (MQ=255)
                                                                                        ttCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCa                                                               >  3:67835/1‑148 (MQ=255)
                                                                                        ttCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCa                                                               <  4:1039861/148‑1 (MQ=255)
                                                                                        ttCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAg                                                              >  1:423286/1‑149 (MQ=255)
                                                                                         tCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAg                                                              >  4:307015/1‑148 (MQ=255)
                                                                                         tCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAg                                                              >  3:823467/1‑148 (MQ=255)
                                                                                         tCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGtt                                                            <  2:356094/149‑1 (MQ=255)
                                                                                          ccATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGtt                                                            <  2:949513/149‑1 (MQ=255)
                                                                                           cATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATggg                                                                                                  >  2:165561/1‑110 (MQ=255)
                                                                                           cATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATggg                                                                                                  <  1:165561/110‑1 (MQ=255)
                                                                                           cATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTc                                                           >  2:566360/1‑149 (MQ=255)
                                                                                              cAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaa                                                         <  3:702005/148‑1 (MQ=255)
                                                                                              cAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaa                                                         <  4:954961/148‑1 (MQ=255)
                                                                                               aGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCTGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAg                                                       <  1:870114/149‑1 (MQ=255)
                                                                                               aGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaaa                                                        <  4:178752/148‑1 (MQ=255)
                                                                                               aGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaaa                                                        <  3:213371/148‑1 (MQ=255)
                                                                                               aGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAg                                                       <  1:610486/149‑1 (MQ=255)
                                                                                               aGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAg                                                       <  2:423286/149‑1 (MQ=255)
                                                                                               aGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAg                                                       <  1:349702/149‑1 (MQ=255)
                                                                                               aGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAg                                                       <  1:776330/149‑1 (MQ=255)
                                                                                                gCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAgg                                                      >  2:324843/1‑149 (MQ=255)
                                                                                                 cGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAggg                                                     <  1:86637/149‑1 (MQ=255)
                                                                                                  ggCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAgggg                                                    <  1:926359/149‑1 (MQ=255)
                                                                                                      tcctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCa                                                                                    <  1:148841/113‑1 (MQ=255)
                                                                                                      tcctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCa                                                                                    >  2:148841/1‑113 (MQ=255)
                                                                                                      tcctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCgca                                                <  2:946419/149‑1 (MQ=255)
                                                                                                      tcctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCgca                                                <  2:434903/149‑1 (MQ=255)
                                                                                                        ctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCgcagc                                              >  2:294119/1‑149 (MQ=255)
                                                                                                           gagaCAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGcac                                            <  3:921535/148‑1 (MQ=255)
                                                                                                            agaCAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACAt                                          <  1:411862/149‑1 (MQ=255)
                                                                                                                  cGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATggg                                                                                                  <  3:546756/87‑1 (MQ=255)
                                                                                                                  cGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATggg                                                                                                  >  4:546756/1‑87 (MQ=255)
                                                                                                                  cGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTc                                     >  3:248781/1‑148 (MQ=255)
                                                                                                                  cGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCa                                    <  2:374430/149‑1 (MQ=255)
                                                                                                                          ccagccagTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAg                            >  1:57381/1‑149 (MQ=255)
                                                                                                                                 gTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAAt                                                                                       <  1:429132/83‑1 (MQ=255)
                                                                                                                                 gTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAAt                                                                                       >  2:429132/1‑83 (MQ=255)
                                                                                                                                 gTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTc                      >  4:597654/1‑148 (MQ=255)
                                                                                                                                    aCCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAgcg                  >  1:235905/1‑149 (MQ=255)
                                                                                                                                     ccGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAgcgc                 >  2:66260/1‑149 (MQ=255)
                                                                                                                                     ccGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAgcg                  >  3:17188/1‑148 (MQ=255)
                                                                                                                                      cGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGcgcg                >  2:447858/1‑149 (MQ=255)
                                                                                                                                        caggagctGGCGAGCCACGCCAGAGCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGt               >  4:210883/7‑148 (MQ=255)
                                                                                                                                            ctctGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATa           <  4:731428/148‑1 (MQ=255)
                                                                                                                                             tctGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATaa          >  3:515822/1‑148 (MQ=255)
                                                                                                                                             tctGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATaa          <  4:823467/148‑1 (MQ=255)
                                                                                                                                             gctGGCGAGCCACGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAg                                                       <  1:509976/102‑1 (MQ=255)
                                                                                                                                                ggCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGt      <  1:99501/149‑1 (MQ=255)
                                                                                                                                                 gCGTGGCTCGCCAGCTCCTGTGCGCTTTTCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                        <  1:881628/66‑1 (MQ=255)
                                                                                                                                                 gCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                        >  2:881628/1‑66 (MQ=255)
                                                                                                                                                   gTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGtt                                                            >  2:414885/1‑92 (MQ=255)
                                                                                                                                                    tGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGc  <  1:447858/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGCGCCAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGC  >  NC_000913/3480514‑3480810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: