Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,480,666 T→A 5.6% S166C (AGC→TGC) ‡ kefG ← potassium‑efflux system ancillary protein for KefB, glutathione‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,480,6660TA 5.6% 376.3 / 9.8 108S166C (AGC→TGC) ‡kefGpotassium‑efflux system ancillary protein for KefB, glutathione‑regulated
Reads supporting (aligned to +/- strand):  ref base T (43/59);  new base A (2/4);  total (45/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01

Only 100 of 112 total aligned reads displayed.

CAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTT  >  NC_000913/3480520‑3480814
                                                                                                                                                  |                                                                                                                                                    
cAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTc                                                                                                                                                     >  4:889870/1‑148 (MQ=255)
 aaTACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCg                                                                                                                                                    >  3:538388/1‑148 (MQ=255)
 aaTACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCg                                                                                                                                                    <  4:428943/148‑1 (MQ=255)
 aaTACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCg                                                                                                                                                    >  4:702005/1‑148 (MQ=255)
     cccAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAga                                                                                                                                               <  2:424319/149‑2 (MQ=255)
      ccAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCCGCt                                                                                                                                              >  2:411862/1‑149 (MQ=255)
      ccAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAga                                                                                                                                               <  3:583778/148‑2 (MQ=255)
      ccAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAga                                                                                                                                               <  3:378067/148‑2 (MQ=255)
         gCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGAGCCACGCCAga                                                                                                                                               >  1:65490/1‑144 (MQ=255)
           cgcgATTCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCtg                                                                                                                                          <  3:232568/148‑1 (MQ=255)
                   cAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCt                                                                                                                                              <  1:545208/136‑1 (MQ=255)
                     gCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGcc                                                                                                                               <  1:53664/149‑1 (MQ=255)
                                 ccACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGt                                                                                                                   >  1:170856/1‑149 (MQ=255)
                                  cACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTa                                                                                                                  <  1:209322/149‑1 (MQ=255)
                                       ccgcGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAaata                                                                                                              >  3:178752/1‑148 (MQ=255)
                                           gAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCt                                                                                                                                                      >  2:638021/1‑104 (MQ=255)
                                           gAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCt                                                                                                                                                      <  1:638021/104‑1 (MQ=255)
                                           gAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgat                                                                                                         <  1:144222/149‑1 (MQ=255)
                                           gAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatga                                                                                                          <  3:389587/148‑1 (MQ=255)
                                            aaGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgatg                                                                                                        <  2:181817/149‑1 (MQ=255)
                                            aaGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgatg                                                                                                        <  1:1028378/149‑1 (MQ=255)
                                             agagAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgatg                                                                                                        <  3:755660/148‑1 (MQ=255)
                                              gagaAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGTCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATgg                                                                                                       <  3:124483/148‑1 (MQ=255)
                                               agaAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGa                                                                                                     <  1:221003/149‑1 (MQ=255)
                                                  aaCAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACtt                                                                                                  >  2:610486/1‑149 (MQ=255)
                                                        aCTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                            <  1:950869/149‑1 (MQ=255)
                                                         cTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                            >  3:260641/1‑148 (MQ=255)
                                                         cTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                            <  4:272181/148‑1 (MQ=255)
                                                         cTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                            >  4:921535/1‑148 (MQ=255)
                                                         cTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGCCTCGCCCAGTAAATAATGATGGGACTTAACCAAt                                                                                           >  1:526464/1‑149 (MQ=255)
                                                         cTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGACACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                            <  3:739901/148‑1 (MQ=255)
                                                          tCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATg                                                                                          >  2:926359/1‑149 (MQ=255)
                                                              gCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTaa                                                                                                <  4:156212/139‑1 (MQ=255)
                                                              gCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTaa                                                                                                >  3:156212/1‑139 (MQ=255)
                                                                gAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCg                                                                                    >  1:782407/1‑149 (MQ=255)
                                                                   tAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGc                                                                                  <  3:579736/148‑1 (MQ=255)
                                                                     aaaaaTCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGcaca                                                                               <  1:389561/149‑1 (MQ=255)
                                                                     aaaaaTCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGcaca                                                                               >  2:776330/1‑149 (MQ=255)
                                                                      aaaaTCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGcaca                                                                               >  4:213371/1‑148 (MQ=255)
                                                                          tCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgat                                                                                                         >  2:452621/1‑118 (MQ=255)
                                                                          tCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAatgat                                                                                                         <  1:452621/118‑1 (MQ=255)
                                                                             gAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGTACATGCccgcc                                                                       <  1:562399/149‑1 (MQ=255)
                                                                              aaCCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCccgcc                                                                       >  4:202497/1‑148 (MQ=255)
                                                                                 cTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTgcgc                                                                                                                                     <  1:104757/83‑1 (MQ=255)
                                                                                 cTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTgcgc                                                                                                                                     >  2:104757/1‑83 (MQ=255)
                                                                                  ttCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCa                                                                   >  3:67835/1‑148 (MQ=255)
                                                                                  ttCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCa                                                                   <  4:1039861/148‑1 (MQ=255)
                                                                                  ttCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAg                                                                  >  1:423286/1‑149 (MQ=255)
                                                                                   tCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAg                                                                  >  3:823467/1‑148 (MQ=255)
                                                                                   tCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAg                                                                  >  4:307015/1‑148 (MQ=255)
                                                                                   tCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGtt                                                                <  2:356094/149‑1 (MQ=255)
                                                                                    ccATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGtt                                                                <  2:949513/149‑1 (MQ=255)
                                                                                     cATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATggg                                                                                                      >  2:165561/1‑110 (MQ=255)
                                                                                     cATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATggg                                                                                                      <  1:165561/110‑1 (MQ=255)
                                                                                     cATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTc                                                               >  2:566360/1‑149 (MQ=255)
                                                                                        cAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaa                                                             <  4:954961/148‑1 (MQ=255)
                                                                                        cAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaa                                                             <  3:702005/148‑1 (MQ=255)
                                                                                         aGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCTGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAg                                                           <  1:870114/149‑1 (MQ=255)
                                                                                         aGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCaaa                                                            <  3:213371/148‑1 (MQ=255)
                                                                                         aGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAg                                                           <  1:349702/149‑1 (MQ=255)
                                                                                         aGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAg                                                           <  2:423286/149‑1 (MQ=255)
                                                                                         aGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAg                                                           <  1:610486/149‑1 (MQ=255)
                                                                                          gCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAgg                                                          >  2:324843/1‑149 (MQ=255)
                                                                                           cGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAggg                                                         <  1:86637/149‑1 (MQ=255)
                                                                                            ggCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAgggg                                                        <  1:926359/149‑1 (MQ=255)
                                                                                                tcctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCa                                                                                        <  1:148841/113‑1 (MQ=255)
                                                                                                tcctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCa                                                                                        >  2:148841/1‑113 (MQ=255)
                                                                                                tcctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCgca                                                    <  2:946419/149‑1 (MQ=255)
                                                                                                tcctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCgca                                                    <  2:434903/149‑1 (MQ=255)
                                                                                                  ctGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCgcagc                                                  >  2:294119/1‑149 (MQ=255)
                                                                                                     gagaCAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGcac                                                <  3:921535/148‑1 (MQ=255)
                                                                                                      agaCAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACAt                                              <  1:411862/149‑1 (MQ=255)
                                                                                                            cGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATggg                                                                                                      <  3:546756/87‑1 (MQ=255)
                                                                                                            cGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATggg                                                                                                      >  4:546756/1‑87 (MQ=255)
                                                                                                            cGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTc                                         >  3:248781/1‑148 (MQ=255)
                                                                                                            cGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCa                                        <  2:374430/149‑1 (MQ=255)
                                                                                                                    ccagccagTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAg                                >  1:57381/1‑149 (MQ=255)
                                                                                                                           gTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAAt                                                                                           <  1:429132/83‑1 (MQ=255)
                                                                                                                           gTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAAt                                                                                           >  2:429132/1‑83 (MQ=255)
                                                                                                                           gTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTc                          >  4:597654/1‑148 (MQ=255)
                                                                                                                               ccGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAgcgc                     >  2:66260/1‑149 (MQ=255)
                                                                                                                               ccGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAgcgc                     <  2:239080/149‑1 (MQ=255)
                                                                                                                               ccGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAgcg                      >  3:17188/1‑148 (MQ=255)
                                                                                                                                cGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGcgcg                    >  2:447858/1‑149 (MQ=255)
                                                                                                                                  caggagctGGCGAGCCACGCCAGAGCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGt                   >  4:210883/7‑148 (MQ=255)
                                                                                                                                      ctctGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATa               <  4:731428/148‑1 (MQ=255)
                                                                                                                                       tctGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATaa              <  4:823467/148‑1 (MQ=255)
                                                                                                                                       tctGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATaa              >  3:515822/1‑148 (MQ=255)
                                                                                                                                          ggCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGt          <  1:99501/149‑1 (MQ=255)
                                                                                                                                           gCGTGGCTCGCCAGCTCCTGTGCGCTTTTCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                            <  1:881628/66‑1 (MQ=255)
                                                                                                                                           gCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCaa                                                                                            >  2:881628/1‑66 (MQ=255)
                                                                                                                                             gTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGtt                                                                >  2:414885/1‑92 (MQ=255)
                                                                                                                                             gTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGtt                                                                <  1:414885/92‑1 (MQ=255)
                                                                                                                                              tGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGc      <  1:447858/149‑1 (MQ=255)
                                                                                                                                               ggCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGc      <  4:260641/148‑1 (MQ=255)
                                                                                                                                                gCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCa     <  3:889870/148‑1 (MQ=255)
                                                                                                                                                 cTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCa                                        >  4:418938/1‑112 (MQ=255)
                                                                                                                                                 cTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCa                                        <  3:418938/112‑1 (MQ=255)
                                                                                                                                                  tCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAGTGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACtt  >  1:922192/1‑149 (MQ=255)
                                                                                                                                                  tCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACtt  >  1:204433/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CAATACCCAGCCGCGATGCCAGCGGCACCGCAGCCACCGCCGCGAAGAGAAACAGCACTCCTGCGAGTAAAAAATCGGAACCTTCCATCAGCGGCCTCCTGGAGACAGCGGATTTGCCAGCCAGTCACCGTAGGCTCTGGCGTGGCTCGCCAGCTCCTGTGCGCTTTGCCGTCTCGCCCAGTAAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTT  >  NC_000913/3480520‑3480814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: