Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,648,273 C→G 7.2% intergenic (+127/‑21) agrA → / → agrB inactive antisense sRNA/antisense sRNA antitoxin for the DinQ toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,648,2730CG 7.2% 384.8 / 18.0 111intergenic (+127/‑21)agrA/agrBinactive antisense sRNA/antisense sRNA antitoxin for the DinQ toxin
Reads supporting (aligned to +/- strand):  ref base C (52/51);  new base G (3/5);  total (55/56)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01

Only 100 of 112 total aligned reads displayed.

ACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAAT  >  NC_000913/3648126‑3648421
                                                                                                                                                   |                                                                                                                                                    
aCGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACt                                                                                                                                                     >  1:530397/1‑149 (MQ=255)
 cGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACt                                                                                                                                                     >  3:209319/1‑148 (MQ=255)
 cGCAAAGTCGGTTTTTTTACATCCGGATTCAGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACt                                                                                                                                                     <  3:861342/148‑1 (MQ=255)
    aaaGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCt                                                                                                                                                 >  1:881810/1‑149 (MQ=255)
     aaGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCt                                                                                                                                                 >  4:812182/1‑148 (MQ=255)
        tCGGTTTGTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATa                                                                                                                                             >  2:919196/1‑149 (MQ=255)
         cGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCAGTGGCTaacat                                                                                                                                            <  1:553618/149‑4 (MQ=255)
          ggTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCAGTGGCTaacatt                                                                                                                                           <  2:154045/149‑5 (MQ=255)
          ggTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCAGTGGCTaacatt                                                                                                                                           <  1:210141/149‑5 (MQ=255)
          ggTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCAGTGGCTaacat                                                                                                                                            <  3:283439/148‑4 (MQ=255)
             ttttttACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTAtt                                                                                                                                        <  1:198633/149‑1 (MQ=255)
               ttttACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGa                                                                                                                                      <  1:425490/149‑1 (MQ=255)
                 ttACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGc                                                                                                                                    >  1:37242/1‑149 (MQ=255)
                  tACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGc                                                                                                                                    <  3:980708/148‑1 (MQ=255)
                     aTCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGtt                                                                                                                                >  2:366855/1‑149 (MQ=255)
                     aTCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGt                                                                                                                                 <  4:779366/148‑1 (MQ=255)
                      tCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTa                                                                                                                               <  2:965952/149‑1 (MQ=255)
                      tCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTa                                                                                                                               >  2:234701/1‑149 (MQ=255)
                       ccGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTa                                                                                                                               >  4:748075/1‑148 (MQ=255)
                       ccGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTa                                                                                                                               >  3:772024/1‑148 (MQ=255)
                        cGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAACGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCTACTGCCTAAAAGTATTGAGCTGTTAg                                                                                                                              >  3:69232/1‑148 (MQ=255)
                            ttCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATaa                                                                                                                          >  4:420140/1‑148 (MQ=255)
                              cgcaCAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGa                                                                                                                        <  3:702603/145‑1 (MQ=255)
                              cGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGaa                                                                                                                       <  2:276120/149‑1 (MQ=255)
                                 aCAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAActc                                                                                                                    <  1:244710/149‑1 (MQ=255)
                                  cAAGGCTTAATATGACGATGACCCAGTGAAAGTATGTAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAActc                                                                                                                    <  3:867970/148‑1 (MQ=255)
                                  cAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAActct                                                                                                                   <  2:881810/149‑1 (MQ=255)
                                   aaGGCGTAATATGACGATGACCCAGTGAAAGTAGATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCGAATAGTATTGAGCTGTTAGATAAGAActctc                                                                                                                  >  1:308420/1‑149 (MQ=255)
                                           atatGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTcca                                                                                                          <  2:373635/149‑1 (MQ=255)
                                             atGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTccagc                                                                                                        >  2:888899/1‑149 (MQ=255)
                                              tGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTccagcc                                                                                                       >  2:935907/1‑149 (MQ=255)
                                              tGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTccagcc                                                                                                       >  2:518153/1‑149 (MQ=255)
                                              tGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTccagcc                                                                                                       >  2:989576/1‑149 (MQ=255)
                                                 cGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTccagccag                                                                                                     <  4:772024/148‑1 (MQ=255)
                                                 cGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCaga                                                                                                    <  2:420973/149‑1 (MQ=255)
                                                     gACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGcca                                                                                                >  1:3496/1‑149 (MQ=255)
                                                      aCCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGccac                                                                                               <  1:248381/149‑1 (MQ=255)
                                                        ccAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAActctct                                                                                                                 <  2:778401/129‑1 (MQ=255)
                                                        ccAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAActctct                                                                                                                 >  1:778401/1‑129 (MQ=255)
                                                         cAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGa                                                                                                                                      <  4:289957/107‑1 (MQ=255)
                                                         cAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGa                                                                                                                                      >  3:289957/1‑107 (MQ=255)
                                                         cAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGccacca                                                                                             >  3:167540/1‑148 (MQ=255)
                                                         cAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCaa                                                                                            <  1:208807/149‑1 (MQ=255)
                                                          aGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGaa                                                                                                                       <  2:467801/121‑1 (MQ=255)
                                                           gTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACt                                                                                          >  1:237872/1‑149 (MQ=255)
                                                            tGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTc                                                                                         >  2:108526/1‑149 (MQ=255)
                                                             gAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTc                                                                                         <  4:434127/148‑1 (MQ=255)
                                                              aaaGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCa                                                                                        <  3:252101/148‑1 (MQ=255)
                                                                aGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGa                                                                                                                             <  3:1009466/109‑1 (MQ=255)
                                                                aGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCACTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGa                                                                                                                             >  4:1009466/1‑109 (MQ=255)
                                                                    tataAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTgg                                                                                 >  1:443517/1‑149 (MQ=255)
                                                                       aaaTCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGaaa                                                                              <  1:469846/149‑1 (MQ=255)
                                                                       aaaTCGTCACTGCGATATATACCGAAGTGCTCCCTACGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGaa                                                                               >  3:575453/1‑148 (MQ=255)
                                                                         aTCGTCACTGCGTTATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAg                                                                             >  3:478935/1‑148 (MQ=255)
                                                                         aTCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAg                                                                             >  3:1037659/1‑148 (MQ=255)
                                                                             ccACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTaaa                                                                         <  3:842788/147‑1 (MQ=255)
                                                                              cACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTaaaa                                                                        >  4:853970/1‑148 (MQ=255)
                                                                                 tGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAAcc                                                                    <  2:748358/149‑1 (MQ=255)
                                                                                 tGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAAc                                                                     <  4:209319/148‑1 (MQ=255)
                                                                                   cGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCg                                                                   <  4:167540/148‑1 (MQ=255)
                                                                                   cGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCg                                                                   <  4:508250/148‑1 (MQ=255)
                                                                                   cGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGa                                                                  <  2:143911/149‑1 (MQ=255)
                                                                                      tatataCCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTGACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGAct                                                                >  3:872027/1‑147 (MQ=255)
                                                                                         ataCCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCaaa                                                            <  2:545901/149‑1 (MQ=255)
                                                                                           aCCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAg                                                           <  3:876901/148‑1 (MQ=255)
                                                                                               aaGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCgg                                                       >  4:166502/1‑148 (MQ=255)
                                                                                                 gTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttt                                                    <  2:237872/149‑1 (MQ=255)
                                                                                                   gCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttt                                                  <  2:308420/149‑1 (MQ=255)
                                                                                                     tccctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttttt                                                >  1:406652/1‑149 (MQ=255)
                                                                                                     tccctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGtttttt                                                 >  3:150704/1‑148 (MQ=255)
                                                                                                       cctccGCCTGCTGAAGCAATCGCTAATGCTTGCAATGTTAGCCACTCGCTAATAGTATTGAGCTGTAAGATCAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGtttttttg                                               >  4:680116/1‑147 (MQ=25)
                                                                                                       cctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCGACTGGCTAATAGTATTGAGATGtt                                                                                                                                <  1:914115/67‑1 (MQ=255)
                                                                                                       cctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGtt                                                                                                                                >  2:34118/1‑67 (MQ=255)
                                                                                                       cctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGtt                                                                                                                                <  1:34118/67‑1 (MQ=255)
                                                                                                       cctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc                                              >  1:1076142/1‑149 (MQ=255)
                                                                                                        ctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttt                                                  >  3:730671/1‑144 (MQ=255)
                                                                                                        ctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttt                                                  <  4:730671/144‑1 (MQ=255)
                                                                                                        ctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc                                              >  3:642188/1‑148 (MQ=255)
                                                                                                         tccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGt                                            <  1:989576/149‑1 (MQ=255)
                                                                                                                   gaagaaATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCa                                   >  3:413505/1‑148 (MQ=255)
                                                                                                                   gaagaaATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAg                                  >  1:113508/1‑149 (MQ=255)
                                                                                                                    aagaaATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGGGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAg                                  >  3:690401/1‑148 (MQ=255)
                                                                                                                          tCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTTAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGActtcct                           >  2:776081/1‑149 (MQ=255)
                                                                                                                          tCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTg                                        <  3:110824/136‑1 (MQ=255)
                                                                                                                           cGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGAcctcct                           <  3:500619/148‑1 (MQ=255)
                                                                                                                           cGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCtt                          <  1:798901/149‑1 (MQ=255)
                                                                                                                              tAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTc                        >  4:212442/1‑148 (MQ=255)
                                                                                                                                   cTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATg                   >  4:771603/1‑148 (MQ=255)
                                                                                                                                    ttGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGaa                 <  2:246641/149‑1 (MQ=255)
                                                                                                                                    ttGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGa                  <  3:533732/148‑1 (MQ=255)
                                                                                                                                    ttGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAACGaa                 <  2:113508/149‑1 (MQ=255)
                                                                                                                                     aatactattAGCCAGTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCaaa                     <  1:385277/137‑1 (MQ=255)
                                                                                                                                     aatactattAGCCAGTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCaaa                     >  2:385277/8‑144 (MQ=255)
                                                                                                                                     aatactattAGCCAGTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAAt                >  1:217558/8‑149 (MQ=255)
                                                                                                                                       tactattAGCCAGTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAg              >  1:776976/6‑149 (MQ=255)
                                                                                                                                            ttAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCaa          >  3:851846/1‑148 (MQ=255)
                                                                                                                                                  aCTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCaaaa   <  2:530397/149‑1 (MQ=255)
                                                                                                                                                  aCTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCaaa    <  3:853970/148‑1 (MQ=255)
                                                                                                                                                  aCTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCaaa    >  3:465617/1‑148 (MQ=255)
                                                                                                                                                   cTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAAt  >  1:92365/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAAT  >  NC_000913/3648126‑3648421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: