Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 448,368 A→T 5.4% V98D (GTT→GAT) ‡ cyoC ← cytochrome o ubiquinol oxidase subunit III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913448,3680AT 5.4% 439.9 / 9.4 128V98D (GTT→GAT) ‡cyoCcytochrome o ubiquinol oxidase subunit III
Reads supporting (aligned to +/- strand):  ref base A (52/68);  new base T (4/3);  total (57/71)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

ACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCACCAGAA  >  NC_000913/448220‑448515
                                                                                                                                                    |                                                                                                                                                   
  cgtgcgtgCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAAcc                                                                                                                                                   <  2:593616/149‑1 (MQ=255)
  cgtgcgtgCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAAc                                                                                                                                                    <  3:851225/148‑1 (MQ=255)
  cgtgcgtgCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAAc                                                                                                                                                    <  4:846619/148‑1 (MQ=255)
   gtgcgtgcCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAAcc                                                                                                                                                   >  4:535520/1‑148 (MQ=255)
    tgcgtgcCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCt                                                                                                                                                  >  3:665774/1‑148 (MQ=255)
    tgcgtgcCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCt                                                                                                                                                  <  3:872480/148‑1 (MQ=255)
     gcgtgcCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTg                                                                                                                                                 <  4:17184/148‑1 (MQ=255)
      cgtgcCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTgg                                                                                                                                                <  3:1047000/148‑1 (MQ=255)
      cgtgcCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGc                                                                                                                                               <  1:507545/149‑1 (MQ=255)
        tgcCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGTTATCTCCTGGCt                                                                                                                                              <  3:525200/148‑1 (MQ=255)
          ccGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCttt                                                                                                                                            <  4:555254/148‑1 (MQ=255)
          ccGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCttt                                                                                                                                            <  3:697422/148‑1 (MQ=255)
           cGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTttg                                                                                                                                          <  1:425986/149‑1 (MQ=255)
            gACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTttgt                                                                                                                                         <  2:30871/149‑1 (MQ=255)
             aCCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCATGTCAACGCCAGCCAGGAGAGAACCTGGCTTttgt                                                                                                                                         <  4:445102/148‑1 (MQ=255)
             aCCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTttgtt                                                                                                                                        <  2:895668/149‑1 (MQ=255)
             aCCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTttgtt                                                                                                                                        <  2:4336/149‑1 (MQ=255)
             aCCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTttgt                                                                                                                                         >  4:270001/1‑148 (MQ=255)
             aCCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTttgt                                                                                                                                         <  4:285286/148‑1 (MQ=255)
             aCCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTttgt                                                                                                                                         <  4:607407/148‑1 (MQ=255)
              ccAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTttgttg                                                                                                                                       <  2:400260/149‑1 (MQ=255)
                 gcgcAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGttt                                                                                                                                    <  1:230407/149‑1 (MQ=255)
                  cgcAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGtttt                                                                                                                                   <  1:329626/149‑1 (MQ=255)
                   gcAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGttttt                                                                                                                                  <  2:28231/149‑1 (MQ=255)
                   gcAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGtttt                                                                                                                                   <  4:665774/148‑1 (MQ=255)
                    cAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGttttt                                                                                                                                  >  3:635751/1‑148 (MQ=255)
                     agaGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTg                                                                                                                                 <  4:254485/146‑1 (MQ=255)
                      aaGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTa                                                                                                                               <  1:792677/149‑1 (MQ=255)
                           cGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATgg                                                                                                                          <  1:142313/149‑1 (MQ=255)
                                   ggAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACTTGGCGATAgc                                                                                                                   <  3:928000/148‑1 (MQ=255)
                                           cTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATAGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGc                                                                                                           >  4:872306/1‑148 (MQ=255)
                                               gATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTa                                                                                                       <  3:1020367/148‑1 (MQ=255)
                                                 tCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAAc                                                                                                                                                    >  1:741340/1‑101 (MQ=255)
                                                     gACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGAt                                                                                                 <  3:43233/148‑1 (MQ=255)
                                                     gACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATg                                                                                                >  1:261137/1‑149 (MQ=255)
                                                         cATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTAc                                                                                                                              >  3:1020028/1‑115 (MQ=255)
                                                          aTGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAg                                                                                             <  2:775100/147‑1 (MQ=255)
                                                          aTGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGc                                                                                            <  4:233768/148‑1 (MQ=255)
                                                           tGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATACCCTGGCTTTTGTTGTTTTTGTACATGGCGCTAGCCGCCCTGCCGTAGGTGATGGAGCt                                                                                           >  4:1058269/1‑148 (MQ=255)
                                                           tGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCt                                                                                           >  4:1057677/1‑148 (MQ=255)
                                                             ccGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGaa                                                                                        <  1:1043592/149‑1 (MQ=255)
                                                               gTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGttttt                                                                                                                                  >  2:445358/1‑105 (MQ=255)
                                                                ttAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGaacaa                                                                                     >  2:451292/1‑149 (MQ=255)
                                                                 tAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGaacaa                                                                                     >  4:805777/1‑148 (MQ=255)
                                                                  aaCAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCACCATGCCGTAGGTGATGGAGCTGaacaac                                                                                    <  4:762093/148‑1 (MQ=255)
                                                                     aaTCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCa                                                                                >  2:88010/1‑149 (MQ=255)
                                                                          ggTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGaaa                                                                           <  2:273654/149‑1 (MQ=255)
                                                                          ggTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGaa                                                                            <  4:397098/148‑1 (MQ=255)
                                                                          gatGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGaa                                                                            >  4:541637/3‑148 (MQ=255)
                                                                               tGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGttt                                                                       <  3:541637/148‑1 (MQ=255)
                                                                               tGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTc                                                                      <  1:798884/149‑1 (MQ=255)
                                                                                    ttCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGtt                                                                        >  1:481675/1‑142 (MQ=255)
                                                                                    ttCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCa                                                                 <  2:918693/149‑1 (MQ=255)
                                                                                     tCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTTTTTTTGTACATGGCGATAGCCGCCATg                                                                                                            >  1:259181/1‑105 (MQ=255)
                                                                                       aTAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAgaa                                                              >  2:80033/1‑149 (MQ=255)
                                                                                       aTAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAgaa                                                              >  2:635166/1‑149 (MQ=255)
                                                                                       aTAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGCGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAgaa                                                              >  2:143781/1‑149 (MQ=255)
                                                                                         aGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAgaac                                                             >  3:730301/1‑148 (MQ=255)
                                                                                            tttCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAgaacgaac                                                         >  1:961586/1‑149 (MQ=255)
                                                                                            tttCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAgaacgaa                                                          <  3:270001/148‑1 (MQ=255)
                                                                                                 accgcGATAACTCCGGCACCATACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGTTGTTGTAGTTTTTGTACATGGCGATAGCCGCCATGCGATAGGTGAAGGAGCTGGACAACAGCGAGAAAGTTTCAACAAGAACGAACGGCAg                                                    <  2:813883/145‑1 (MQ=255)
                                                                                                  tCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAg                                                    >  4:643337/1‑148 (MQ=255)
                                                                                                  tCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAg                                                    >  4:1020962/1‑148 (MQ=255)
                                                                                                  tCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAg                                                    >  3:648636/1‑148 (MQ=255)
                                                                                                      gATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCg                                                <  3:950857/148‑1 (MQ=255)
                                                                                                        tAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTTGGGGATGGAGCTGAACAACAGCAAGAAAGGTTCAACCAGAACGAACGGCAGTTCGaa                                              >  3:827238/1‑148 (MQ=255)
                                                                                                         aaaTCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGaaa                                             >  4:435797/1‑148 (MQ=255)
                                                                                                              cGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAg                                                                >  3:1039488/1‑124 (MQ=255)
                                                                                                              cGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAg                                                                <  4:1039488/124‑1 (MQ=255)
                                                                                                              cGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTcc                                       <  1:1017731/149‑1 (MQ=255)
                                                                                                              cGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTc                                        <  4:679411/148‑1 (MQ=255)
                                                                                                                  aCCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTa                                    <  3:12650/148‑1 (MQ=255)
                                                                                                                  aCCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTAc                                   <  2:1060566/149‑1 (MQ=255)
                                                                                                                   ccAAACAACCAGGTCAACGCCAGCCAGTAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCTTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTAc                                   <  3:1058269/148‑1 (MQ=255)
                                                                                                                    cAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCt                                 >  1:121725/1‑149 (MQ=255)
                                                                                                                     aaaCAATCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAGCAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCt                                 >  4:895388/1‑148 (MQ=255)
                                                                                                                     aaaCAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGaaaa                                            >  1:1001217/1‑137 (MQ=255)
                                                                                                                     aaaCAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGaaaa                                            <  2:1001217/137‑1 (MQ=255)
                                                                                                                     aaaCAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTg                                >  2:735365/1‑149 (MQ=255)
                                                                                                                      aacaacCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCaa                                                                    >  1:365620/1‑112 (MQ=255)
                                                                                                                      aacaacCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCaa                                                                    <  2:365620/112‑1 (MQ=255)
                                                                                                                            cAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGgcc                         >  1:64061/1‑149 (MQ=255)
                                                                                                                                tCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCt                     >  2:57161/1‑149 (MQ=255)
                                                                                                                                  aaCGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGc                   >  1:969098/1‑149 (MQ=255)
                                                                                                                                   acaaaagccagGTTATCTCCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACCACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGgccgc                       <  2:1060774/139‑1 (MQ=255)
                                                                                                                                   acaaaagccagGTTATCTCCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGgccgc                       >  1:1060774/6‑144 (MQ=255)
                                                                                                                                   aCGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGc                   >  3:483357/1‑148 (MQ=255)
                                                                                                                                   aCGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCg                  <  2:724044/149‑1 (MQ=255)
                                                                                                                                    caaaagccagGTTATCTCCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCgg                 >  2:862004/5‑149 (MQ=255)
                                                                                                                                     aaaagccagGTTATCTCCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCaa                                                                               >  1:536432/4‑86 (MQ=38)
                                                                                                                                       cagccagGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGc              >  1:808080/1‑149 (MQ=255)
                                                                                                                                            agGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGtt          >  4:237807/1‑148 (MQ=255)
                                                                                                                                            agGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGtt          >  3:741295/1‑148 (MQ=255)
                                                                                                                                             ggAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGaaaa                                            <  1:595843/113‑1 (MQ=255)
                                                                                                                                             ggAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTc         >  3:807204/1‑148 (MQ=255)
                                                                                                                                               agaTAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAAt                                           <  1:421986/112‑1 (MQ=255)
                                                                                                                                               agaTAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAAt                                           >  2:421986/1‑112 (MQ=255)
                                                                                                                                               agaTAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCAcc      <  1:854575/149‑1 (MQ=255)
                                                                                                                                                   aaCCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCACCAGaa  <  2:96756/149‑1 (MQ=255)
                                                                                                                                                   aaCCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCACCAGaa  <  2:550352/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCACCAGAA  >  NC_000913/448220‑448515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: