Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 448,965 G→A 7.3% H559H (CAC→CAT) ‡ cyoB ← cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913448,9650GA 7.3% 385.3 / 13.2 121H559H (CAC→CAT) ‡cyoBcytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base G (56/56);  new base A (2/7);  total (58/63)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 123 total aligned reads displayed.

CCAGATCATGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGA  >  NC_000913/448818‑449113
                                                                                                                                                   |                                                                                                                                                    
ccAGATCATGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCg                                                                                                                                                      >  3:312975/1‑148 (MQ=255)
ccAGATCATGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGt                                                                                                                                                     <  1:648548/149‑1 (MQ=255)
ccAGATCATGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGt                                                                                                                                                     >  1:165006/1‑149 (MQ=255)
ccAGATCATGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGt                                                                                                                                                     >  1:33796/1‑149 (MQ=255)
 cAGATCATGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCAGCACGTTCGTg                                                                                                                                                    <  2:570119/149‑1 (MQ=255)
  aGATCATGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTg                                                                                                                                                    >  4:515222/1‑148 (MQ=255)
        tGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGc                                                                                                                                             <  1:264877/149‑1 (MQ=255)
        tGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCACGAACGTga                                                                                                                                             <  2:909745/149‑2 (MQ=255)
        tGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCACGAACGTg                                                                                                                                              <  3:589638/148‑1 (MQ=255)
         ggCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGGATGCATCACGTTCACGAACGTga                                                                                                                                             <  4:1026141/148‑2 (MQ=255)
         ggCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGACACCTGCACCACTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCACGAACGtaa                                                                                                                                             <  4:347972/148‑3 (MQ=255)
          gCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCACGAACGTgat                                                                                                                                            <  3:806813/148‑3 (MQ=255)
                    aGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCa                                                                                                                                 >  1:396443/1‑149 (MQ=255)
                         gtggAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGtt                                                                                                                            <  2:320545/149‑1 (MQ=255)
                          tggAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTta                                                                                                                           <  1:646949/149‑1 (MQ=255)
                            gagaAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTtat                                                                                                                          <  3:515222/148‑1 (MQ=255)
                               aaaGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGa                                                                                                                       >  3:921962/1‑148 (MQ=255)
                                   cTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGa                                                                                                                  <  2:624603/149‑1 (MQ=255)
                                   cTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGa                                                                                                                  <  1:241927/149‑1 (MQ=255)
                                    tGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAg                                                                                                                 <  2:103519/149‑1 (MQ=255)
                                     gCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAAc                                                                                                                     <  2:527525/144‑1 (MQ=255)
                                     gCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAAc                                                                                                                     >  1:527525/1‑144 (MQ=255)
                                       aaTGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCg                                                                                                              <  1:728014/149‑1 (MQ=255)
                                        aTGAAGATACCTGCACCGCTGTATTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCg                                                                                                              >  4:774839/1‑148 (MQ=255)
                                         tGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCggg                                                                                                            >  2:502560/1‑149 (MQ=255)
                                         tGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCgg                                                                                                             >  3:987545/1‑148 (MQ=255)
                                          gACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGtt                                                                                                                            >  2:509204/1‑132 (MQ=255)
                                          gACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGtt                                                                                                                            <  1:509204/132‑1 (MQ=255)
                                            cGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGaa                                                                                                         <  1:1014534/149‑1 (MQ=255)
                                            cGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGaa                                                                                                         <  2:448336/149‑1 (MQ=255)
                                             gATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCACTTCCCAGAATGCATCACGTTAGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAag                                                                                                        >  2:751536/1‑149 (MQ=255)
                                               tACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAaga                                                                                                       <  4:369142/148‑1 (MQ=255)
                                                  cTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGt                                                                                                    <  4:130588/148‑1 (MQ=255)
                                                    gCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTtata                                                                                                                         >  2:613558/1‑125 (MQ=255)
                                                    gCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGc                                                                                                 <  1:1029758/149‑1 (MQ=255)
                                                    gCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGc                                                                                                 <  1:1010989/149‑1 (MQ=255)
                                                     cACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGcc                                                                                                <  2:140119/149‑1 (MQ=255)
                                                         gCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCGTTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCAc                                                                                             >  3:736916/1‑148 (MQ=255)
                                                           tGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTGTAGAACGGAGGCGGGGAAGAGGTTGCCCACTcc                                                                                          >  1:787688/1‑149 (MQ=255)
                                                           tGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTcc                                                                                          >  1:1035962/1‑149 (MQ=255)
                                                                    gCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACg                                                                                  <  4:447930/148‑1 (MQ=255)
                                                                       tatGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGcc                                                                               >  3:360654/1‑148 (MQ=255)
                                                                          gAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCAcc                                                                            >  4:426385/1‑148 (MQ=255)
                                                                              ttcttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCAc                                                                        >  3:1004751/1‑148 (MQ=255)
                                                                                 ttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGtt                                                                                                   >  3:794898/1‑118 (MQ=255)
                                                                                 ttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGtt                                                                                                   <  4:794898/118‑1 (MQ=255)
                                                                                  tcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTc                                                                   >  2:1028857/1‑149 (MQ=255)
                                                                                     tAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGc                                                                 >  4:454410/1‑148 (MQ=255)
                                                                                      aGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCa                                                               <  2:504046/149‑1 (MQ=255)
                                                                                        tGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGt                                                             <  2:1035962/149‑1 (MQ=255)
                                                                                         ggTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGt                                                             <  3:855277/148‑1 (MQ=255)
                                                                                           tCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCAATCCAGCGTACGGCCACCCCACGGGTCGCCAGTCa                                                           >  3:865171/1‑148 (MQ=255)
                                                                                            cAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAg                                                          >  4:527971/1‑148 (MQ=255)
                                                                                             aGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGt                                                        >  1:290988/1‑149 (MQ=255)
                                                                                                cTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTGTAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCAc                                                     <  2:787688/149‑1 (MQ=255)
                                                                                                     tataCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAGAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGtt                                                 <  4:628090/148‑1 (MQ=255)
                                                                                                      ataCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCt                                               >  1:459662/1‑149 (MQ=255)
                                                                                                        aCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCaa                                                                                                                                >  4:1013835/1‑66 (MQ=255)
                                                                                                        aCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCaa                                                                                                                                <  3:1013835/66‑1 (MQ=255)
                                                                                                           cTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtc                                           >  4:1052996/1‑148 (MQ=255)
                                                                                                            ttCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcg                                          <  4:240533/148‑1 (MQ=255)
                                                                                                            ttCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcg                                          <  4:1004751/148‑1 (MQ=255)
                                                                                                                   ttCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCAc                                                     <  3:986175/130‑1 (MQ=255)
                                                                                                                      tctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAAt                               >  2:482780/1‑149 (MQ=255)
                                                                                                                        tttCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAg                             >  1:415654/1‑149 (MQ=255)
                                                                                                                        attCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAg                             >  2:386257/2‑149 (MQ=255)
                                                                                                                         ttCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGt                                                                                     >  2:1015767/1‑92 (MQ=255)
                                                                                                                         ttCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGt                                                                                     <  1:1015767/92‑1 (MQ=255)
                                                                                                                         ttCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAg                             >  4:1035336/1‑148 (MQ=255)
                                                                                                                          tCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATa                              <  2:438088/146‑1 (MQ=255)
                                                                                                                          tCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATa                              >  1:438088/1‑146 (MQ=255)
                                                                                                                           cATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTATTCGCGACCGCGACCAGaac                          <  2:165006/149‑2 (MQ=255)
                                                                                                                            aTTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAAc                         <  1:502560/149‑1 (MQ=255)
                                                                                                                             tttCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGc                                                                                                 <  3:609002/76‑1 (MQ=255)
                                                                                                                             tttCCCAGAATGCATCACGTTCGTGAACGTGCCGCACTACGGCCAAGTTATTGAACGGAGGCGGGGAAGAGGTGGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAAc                         >  4:464502/1‑148 (MQ=255)
                                                                                                                              ttCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACg                        >  3:982623/1‑148 (MQ=255)
                                                                                                                              ttCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACg                        >  4:554176/1‑148 (MQ=255)
                                                                                                                                cccAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTAc                     >  1:457853/1‑149 (MQ=255)
                                                                                                                                 ccAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTAc                     >  4:28264/1‑148 (MQ=255)
                                                                                                                                  cAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACa                    >  4:713071/1‑148 (MQ=255)
                                                                                                                                  cAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACAt                   >  1:866185/1‑149 (MQ=255)
                                                                                                                                   aGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATc                  <  1:450234/149‑1 (MQ=255)
                                                                                                                                     aaTGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTg                >  2:76273/1‑149 (MQ=255)
                                                                                                                                     aaTGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTg                <  2:395025/149‑1 (MQ=255)
                                                                                                                                          aTCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGTACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACTCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATaa           >  1:312783/1‑149 (MQ=255)
                                                                                                                                          aTCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATaa           >  2:731085/1‑149 (MQ=255)
                                                                                                                                           tCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGcc                                                                               >  3:1000469/1‑80 (MQ=255)
                                                                                                                                           tCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAc          <  1:523474/149‑1 (MQ=255)
                                                                                                                                           tCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAc          <  2:154371/149‑1 (MQ=255)
                                                                                                                                           tCACGTTCGTGAACGTGCGGCACCACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATaa           <  4:736916/148‑1 (MQ=255)
                                                                                                                                            cACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAc          >  4:859646/1‑148 (MQ=255)
                                                                                                                                            cACGTTCACGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAAt                               <  3:123886/127‑1 (MQ=255)
                                                                                                                                              cGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGa        <  4:730651/148‑1 (MQ=255)
                                                                                                                                               gTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTTGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAg       <  4:813830/148‑1 (MQ=255)
                                                                                                                                               gTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAgg      <  2:396443/149‑1 (MQ=255)
                                                                                                                                                ttCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACg                                                                       >  4:479880/1‑83 (MQ=255)
                                                                                                                                                ttCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACg                                                                       <  3:479880/83‑1 (MQ=255)
                                                                                                                                                 tCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGc     >  3:223763/1‑148 (MQ=255)
                                                                                                                                                 tCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCa    <  2:818621/149‑1 (MQ=255)
                                                                                                                                                   gTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCaga  >  1:267430/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCAGATCATGGCGAAACCGAAGATGGTGGAGAAAGCTGCAATGACGATACCTGCACCGCTGTTTTTCGGCATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGA  >  NC_000913/448818‑449113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: