Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,000,244 G→C 6.2% intergenic (+99/+48) uvrD → / ← yigE DNA‑dependent ATPase I and helicase II/DUF2233 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,000,2440GC 6.2% 341.9 / 11.4 97intergenic (+99/+48)uvrD/yigEDNA‑dependent ATPase I and helicase II/DUF2233 family protein
Reads supporting (aligned to +/- strand):  ref base G (45/46);  new base C (3/3);  total (48/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 105 total aligned reads displayed.

GGGTATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCAAGA  >  NC_000913/4000097‑4000391
                                                                                                                                                   |                                                                                                                                                   
gggTATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTAcgccg                                                                                                                                                     <  3:521299/148‑1 (MQ=255)
    aTTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATc                                                                                                                                                 >  3:626785/1‑148 (MQ=255)
    aTTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATc                                                                                                                                                 >  4:1010709/1‑148 (MQ=255)
     ttAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATGCGGCGTAAACGCCCTATCCg                                                                                                                                               <  2:15590/149‑1 (MQ=255)
     ttAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCg                                                                                                                                               >  1:516991/1‑149 (MQ=255)
         aTGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATGCGGCGTAAACGCCCTATCCGGCac                                                                                                                                           >  1:747060/1‑148 (MQ=255)
         aTGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCa                                                                                                                                            >  4:175137/1‑148 (MQ=255)
          tGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCAt                                                                                                                                           <  3:121520/148‑1 (MQ=255)
          tGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCAt                                                                                                                                           <  4:611624/148‑1 (MQ=255)
              tggtggCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATc                                                                                                                                                 <  2:489578/138‑1 (MQ=255)
              tggtggCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATc                                                                                                                                                 >  1:489578/1‑138 (MQ=255)
                  ggcggcATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCAt                                                                                                                                  <  2:189860/149‑1 (MQ=255)
                      gcATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCCTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGAGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTc                                                                                                                              >  2:641674/1‑149 (MQ=255)
                               cGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGa                                                                                                                     <  2:483293/149‑1 (MQ=255)
                                ggCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGac                                                                                                                    >  1:848834/1‑149 (MQ=255)
                                     gAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACaa                                                                                                                >  4:80955/1‑148 (MQ=255)
                                     gAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACaa                                                                                                                >  4:448315/1‑148 (MQ=255)
                                              tAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGt                                                                                                       <  3:135372/148‑1 (MQ=255)
                                                  gTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTaa                                                                                                  <  1:443655/149‑1 (MQ=255)
                                                   ttGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAAc                                                                                                 <  2:114012/149‑1 (MQ=255)
                                                   ttGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATCGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAAc                                                                                                 <  1:769026/149‑1 (MQ=255)
                                                     gCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGttt                                                                                                     <  4:552942/143‑1 (MQ=255)
                                                     gCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGttt                                                                                                     >  3:552942/1‑143 (MQ=255)
                                                             cGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCt                                                                                        <  3:80955/148‑1 (MQ=255)
                                                               gTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGa                                                                                     >  1:529726/1‑149 (MQ=255)
                                                                tgctgcGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGa                                                                                     <  4:626785/148‑1 (MQ=255)
                                                                tgctgcGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGAc                                                                                    >  2:78101/1‑149 (MQ=255)
                                                                     cgcACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTaa                                                                                                  <  3:738175/130‑1 (MQ=255)
                                                                     cgcACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTaa                                                                                                  >  4:738175/1‑130 (MQ=255)
                                                                       cACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAAt                                                                              <  4:34730/148‑1 (MQ=255)
                                                                        aCCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATc                                                                             <  4:896487/148‑1 (MQ=255)
                                                                          cTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCAt                                                                           <  4:643894/148‑1 (MQ=255)
                                                                          cTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATg                                                                          <  2:652716/149‑1 (MQ=255)
                                                                          cTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCACTCGACGGAAATCATg                                                                          >  1:756859/1‑149 (MQ=255)
                                                                           ttATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATg                                                                          <  3:601614/148‑1 (MQ=255)
                                                                           ttATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATg                                                                          >  3:513896/1‑148 (MQ=255)
                                                                            tATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGt                                                                        <  2:407415/149‑1 (MQ=255)
                                                                            tATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGt                                                                        <  2:516991/149‑1 (MQ=255)
                                                                              tttGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGCATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGa                                                                      >  1:276848/1‑149 (MQ=255)
                                                                                   ccTAAAAAATTATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGttt                                                                                                     >  2:187800/1‑113 (MQ=255)
                                                                                   ccTAAAAAATTATTCAGAGTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGttt                                                                                                     <  1:187800/113‑1 (MQ=255)
                                                                                   ccTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACaaaa                                                                 >  2:566656/1‑149 (MQ=255)
                                                                                   ccTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACaaa                                                                  <  4:513896/148‑1 (MQ=255)
                                                                                     tAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTc                                                                                            <  2:482954/120‑1 (MQ=255)
                                                                                     tAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTc                                                                                            >  1:482954/1‑120 (MQ=255)
                                                                                     tAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAgg                                                               >  2:29784/1‑149 (MQ=255)
                                                                                     tAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAgg                                                               >  1:569345/1‑149 (MQ=255)
                                                                                      aaaaaaTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAgg                                                               >  3:332366/1‑148 (MQ=255)
                                                                                       aaaaaTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCAt                                                                           >  4:358276/1‑135 (MQ=255)
                                                                                        aaaaTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGAt                                                             >  3:140932/1‑148 (MQ=255)
                                                                                         aaaTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATa                                                            >  4:204071/1‑148 (MQ=255)
                                                                                          caTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCt                                                                                                                                         >  2:911506/2‑70 (MQ=255)
                                                                                          aaTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCt                                                                                                                                         <  1:911506/70‑1 (MQ=255)
                                                                                          aaTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAg                                                           <  3:448315/148‑1 (MQ=255)
                                                                                          aaTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGCATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGc                                                          <  2:276848/149‑1 (MQ=255)
                                                                                           aTCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCCTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGc                                                          >  3:375364/1‑148 (MQ=255)
                                                                                             cATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGtt                                                       <  1:740139/149‑1 (MQ=255)
                                                                                                  aGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCAc                                                  >  1:519674/1‑149 (MQ=255)
                                                                                                    aTTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACgg                                                <  1:78101/149‑1 (MQ=255)
                                                                                                        aaTAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGAtt                                            >  2:930160/1‑149 (MQ=255)
                                                                                                         aTAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGAtt                                            >  4:86916/1‑148 (MQ=255)
                                                                                                         aTAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGAtt                                            >  3:687110/1‑148 (MQ=255)
                                                                                                         aTAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTg                                           >  1:827063/1‑149 (MQ=255)
                                                                                                          tAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATc                                                                                                         <  1:874062/86‑1 (MQ=255)
                                                                                                          tAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATc                                                                                                         >  2:874062/1‑86 (MQ=255)
                                                                                                           aaaTTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATg                                                                          >  3:283634/1‑116 (MQ=255)
                                                                                                           aaaTTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATg                                                                          <  4:283634/116‑1 (MQ=255)
                                                                                                            aaTTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGccc                                        <  1:182130/149‑1 (MQ=255)
                                                                                                              ttGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGcccc                                       >  3:584179/1‑148 (MQ=255)
                                                                                                                    cGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCttt                                 >  3:797872/1‑148 (MQ=255)
                                                                                                                    cGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTc                                <  2:827063/149‑1 (MQ=255)
                                                                                                                          gTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATAc                          >  2:711321/1‑149 (MQ=255)
                                                                                                                           gatgCCGGATGCGGCGTAAACGCCCTATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACaaa                                                                  >  3:857936/4‑108 (MQ=37)
                                                                                                                           gatgCCGGATGCGGCGTAAACGCCCTATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACaaa                                                                  <  4:857936/105‑1 (MQ=37)
                                                                                                                            aGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACat                        >  2:84671/1‑149 (MQ=255)
                                                                                                                             ggCCGGATAGGGCGCTTACGCCGCATCCGGCACCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACat                        <  3:1013746/148‑1 (MQ=255)
                                                                                                                              gCCGGATGCGGCGTAAACGCCCTATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGcc                                    >  2:744243/1‑135 (MQ=255)
                                                                                                                              gCCGGATGCGGCGTAAACGCCCTATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGcc                                    <  1:744243/135‑1 (MQ=255)
                                                                                                                              gCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACatat                      <  1:633132/149‑1 (MQ=255)
                                                                                                                               ccGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACatat                      >  3:496336/1‑148 (MQ=255)
                                                                                                                                  gATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCCTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATAtgtg                   >  3:41007/1‑148 (MQ=255)
                                                                                                                                    tAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGaa                 <  4:797872/148‑1 (MQ=255)
                                                                                                                                      gggCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAAt               <  3:175137/148‑1 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACatat                      >  2:869439/1‑139 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACatat                      <  1:869439/139‑1 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCg             <  3:101533/148‑1 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGt            <  2:268625/149‑1 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGt            >  1:713581/1‑149 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGt            <  1:631779/149‑1 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCACCCGGCATCTGCGCCATCTTCAGTATCTGACACCAAACTATCGTTTTAACCTTTCCGCTCGACGGATATCATGGTGACAAAAGGATAGCGGTGCCACGGGATTGCGCCGCCTTTCATATCCATATGTGAAATCg             <  4:47984/148‑1 (MQ=255)
                                                                                                                                          gTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTg           <  3:494265/148‑1 (MQ=255)
                                                                                                                                          gTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGc          <  2:75880/149‑1 (MQ=255)
                                                                                                                                           tttACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACACAACTATCGTTTTAACCTTTCCACTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGcc         <  2:756859/149‑1 (MQ=255)
                                                                                                                                            ttACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGcc         >  3:580007/1‑148 (MQ=255)
                                                                                                                                                gccgcATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCaa    <  1:84671/149‑1 (MQ=255)
                                                                                                                                                gccgcATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTCCATCAAg   >  2:344406/1‑149 (MQ=255)
                                                                                                                                                gccgcATCCGGCATCTGAGCCATCTTAAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCttt                                 <  3:911591/120‑1 (MQ=255)
                                                                                                                                                gccgcATCAGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCttt                                 >  4:911591/1‑120 (MQ=255)
                                                                                                                                                 ccgcATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCaa    >  4:798148/1‑148 (MQ=255)
                                                                                                                                                 ccgcATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCaa    <  3:792196/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGGTATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTGTAACGTTGCCGGATGCGGTGCTGCGCACCTTATTTGGCCTAAAAAATCATTCAGATTCAATAAATTGCAACGTCATGTAGGCCGGATAGGGCGTTTACGCCGCATCCGGCATCTGCGCCATCTTCAGTATCTGACACAAAACTATCGTTTTAACCTTTCCGCTCGACGGAAATCATGGTGACAAAAGGATAGCGTTGCCACGGGATTGCCCCGCCTTTCATATACATATGTGAAATCGTGCCATCAAGA  >  NC_000913/4000097‑4000391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: