Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,315,437 A→T 5.3% F34L (TTT→TTA) ‡ phnO ← aminoalkylphosphonate N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,315,4370AT 5.3% 472.6 / 11.9 133F34L (TTT→TTA) ‡phnOaminoalkylphosphonate N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (59/67);  new base T (2/5);  total (61/72)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 142 total aligned reads displayed.

CACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTCGACCGA  >  NC_000913/4315290‑4315584
                                                                                                                                                   |                                                                                                                                                   
caccaACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGaa                                                                                                                                                    >  1:900397/1‑149 (MQ=255)
caccaACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGaa                                                                                                                                                    >  2:788559/1‑149 (MQ=255)
 accaACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGaaa                                                                                                                                                   >  2:208445/1‑149 (MQ=255)
 accaACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGaaa                                                                                                                                                   <  2:258780/149‑1 (MQ=255)
 accaACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGaaa                                                                                                                                                   <  1:234631/149‑1 (MQ=255)
   caACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAAcg                                                                                                                                                 >  1:540215/1‑149 (MQ=255)
   caACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAAc                                                                                                                                                  >  4:601715/1‑148 (MQ=255)
    aaCTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAAcgc                                                                                                                                                <  2:700734/149‑1 (MQ=255)
       tCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCgtg                                                                                                                                             <  2:351016/149‑1 (MQ=255)
       tCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCgtg                                                                                                                                             <  1:393149/149‑1 (MQ=255)
       tCCTGAACTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGTGTTAAAACCCACGCGAAACGCgtg                                                                                                                                             >  2:701717/1‑149 (MQ=255)
        ccTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGTTTCGCgtgg                                                                                                                                            <  1:589977/149‑1 (MQ=255)
        ccTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCgtg                                                                                                                                             >  3:921376/1‑148 (MQ=255)
          tGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCgtggt                                                                                                                                           >  3:86539/1‑148 (MQ=255)
          tGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCgtggt                                                                                                                                           <  3:731370/148‑1 (MQ=255)
              tttCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCa                                                                                                                                      >  2:502323/1‑149 (MQ=255)
               ttCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGTTTCGCgtgggttttaa                                                                                                                                     <  1:112774/149‑8 (MQ=255)
                   ccGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGTTTCGCgtg                                                                                                                                             <  3:249421/137‑1 (MQ=255)
                   ccGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACt                                                                                                                                  >  3:59487/1‑148 (MQ=255)
                   ccGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACt                                                                                                                                  >  4:521438/1‑148 (MQ=255)
                      aTCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGc                                                                                                                              <  1:902661/149‑1 (MQ=255)
                       tCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGcc                                                                                                                             <  2:477377/149‑1 (MQ=255)
                       tCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGc                                                                                                                              >  4:474073/1‑148 (MQ=255)
                        ccAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGcc                                                                                                                             <  3:979357/148‑1 (MQ=255)
                           gTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGtt                                                                                                                         <  2:268171/149‑1 (MQ=255)
                            ttGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGttt                                                                                                                        <  2:540215/149‑1 (MQ=255)
                            ttGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGttt                                                                                                                        <  2:228725/149‑1 (MQ=255)
                              gACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGtttt                                                                                                                       <  4:178921/148‑1 (MQ=255)
                                      gCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTcaca                                                                                                               >  4:588411/1‑148 (MQ=255)
                                         gATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAAt                                                                                                            >  3:714250/1‑148 (MQ=255)
                                          aTGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGttt                                                                                                                        >  4:788640/1‑135 (MQ=255)
                                          aTGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATc                                                                                                           <  3:588411/148‑1 (MQ=255)
                                            gAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAgc                                                                                                        >  4:19686/1‑148 (MQ=255)
                                              aaCTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAgcgc                                                                                                      <  1:54944/149‑1 (MQ=255)
                                                 tGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTa                                                                                                   >  2:981348/1‑149 (MQ=255)
                                                  gCAAATGCAGGCCGATCATGCCGACAACTTCGTCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTa                                                                                                   >  3:10500/1‑148 (MQ=255)
                                                    aaaTGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCaa                                                                                                                                     >  2:939019/1‑112 (MQ=255)
                                                    aaaTGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCaa                                                                                                                                     <  1:939019/112‑1 (MQ=255)
                                                    aaaTGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAAc                                                                                                >  2:158199/1‑149 (MQ=255)
                                                    aaaTGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAAc                                                                                                >  2:882327/1‑149 (MQ=255)
                                                    aaaTGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAAc                                                                                                >  2:542449/1‑149 (MQ=255)
                                                    aaaTGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAAc                                                                                                >  1:1019388/1‑149 (MQ=255)
                                                     aaTGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCATAAATCAGCGCGTAAAc                                                                                                >  3:902259/1‑148 (MQ=255)
                                                                       cGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTg                                                                              <  4:86539/148‑1 (MQ=255)
                                                                          cAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTg                                                                          <  1:438988/149‑1 (MQ=255)
                                                                            aCTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAg                                                                                                         <  4:295216/116‑1 (MQ=255)
                                                                            aCTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAg                                                                                                         >  3:295216/1‑116 (MQ=255)
                                                                             cTTCGCCATAAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGcc                                                                       >  1:924011/1‑149 (MQ=255)
                                                                               tCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCg                                                                      <  3:608354/148‑1 (MQ=255)
                                                                                cGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCgg                                                                     <  3:621068/148‑1 (MQ=255)
                                                                                   cATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGa                                                                 >  2:571206/1‑149 (MQ=255)
                                                                                    aTCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGaaa                                                                                                                                                   >  3:1007062/1‑66 (MQ=255)
                                                                                    aTCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGaaa                                                                                                                                                   <  4:1007062/66‑1 (MQ=255)
                                                                                    aTCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGa                                                                 <  3:1056711/148‑1 (MQ=255)
                                                                                    aTCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGa                                                                 <  3:350619/148‑1 (MQ=255)
                                                                                     tCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGTTTCGCgtggg                                                                                                                                           <  3:279980/73‑2 (MQ=255)
                                                                                         gcagcGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTca                                                           <  1:158199/149‑1 (MQ=255)
                                                                                          cagcGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTca                                                           >  3:271329/1‑148 (MQ=255)
                                                                                            gcgcCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACaa                                                        <  2:65040/149‑1 (MQ=255)
                                                                                             cgcCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGACAAACTCCGCCTGTTTTAGGTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCAGaa                                                        <  4:902259/148‑1 (MQ=255)
                                                                                              gcCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGc                                                      <  2:684797/149‑1 (MQ=255)
                                                                                              gcCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGc                                                      >  2:382155/1‑149 (MQ=255)
                                                                                               ccAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCa                                                     >  1:354313/1‑149 (MQ=255)
                                                                                                cAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCa                                                     <  4:229277/148‑1 (MQ=255)
                                                                                                cAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCa                                                     >  4:1010716/1‑148 (MQ=255)
                                                                                                cAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAg                                                    <  1:157437/149‑1 (MQ=255)
                                                                                                 aGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCt                                                                                                                   >  3:1008714/1‑85 (MQ=255)
                                                                                                  gATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAAcc                                                                                               >  1:1031627/1‑104 (MQ=255)
                                                                                                  gATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAAcc                                                                                               <  2:1031627/104‑1 (MQ=255)
                                                                                                    tGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCa                                                 <  3:894683/148‑1 (MQ=255)
                                                                                                       tAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATgg                                              <  4:410458/148‑1 (MQ=255)
                                                                                                         gcgcATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGgtg                                            <  3:474073/148‑1 (MQ=255)
                                                                                                          cgcATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGgtgt                                           >  3:763624/1‑148 (MQ=255)
                                                                                                           gcATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGtt                                          >  3:137772/1‑148 (MQ=255)
                                                                                                           gcATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGAGGCCGGGCGAAGCTCACAAGCAGGCATGGTGttt                                         >  1:881165/1‑149 (MQ=255)
                                                                                                            cATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGttt                                         >  3:339929/1‑148 (MQ=255)
                                                                                                            cATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTtc                                        <  1:788559/149‑1 (MQ=255)
                                                                                                             gtGTTTGGGTCGCGCAGATTGGCGTTAAAACCCGCGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTtc                                        <  3:421203/147‑1 (MQ=255)
                                                                                                             aTGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTtct                                       <  1:238495/149‑1 (MQ=255)
                                                                                                              tGTTTGGGTCGCGCAGATTGGCGTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTtctc                                      <  3:19686/148‑1 (MQ=255)
                                                                                                                  tGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCtt                                   <  3:521438/148‑1 (MQ=255)
                                                                                                                   gggTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCt                                 <  1:520869/149‑1 (MQ=255)
                                                                                                                    ggTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCt                                 >  4:563452/1‑148 (MQ=255)
                                                                                                                        gcgcAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATgg                            >  2:512425/1‑149 (MQ=255)
                                                                                                                         cgcAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGa                           >  1:850314/1‑149 (MQ=255)
                                                                                                                          gcAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGAt                          <  2:896332/149‑1 (MQ=255)
                                                                                                                              aTTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAg                       <  3:997958/148‑1 (MQ=255)
                                                                                                                                    gTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTc                                  >  1:347765/1‑131 (MQ=255)
                                                                                                                                      tAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTcagca               >  3:233279/1‑148 (MQ=255)
                                                                                                                                         aaCCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCAAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCgt           <  2:924011/149‑1 (MQ=255)
                                                                                                                                           cccACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCt                                                             <  1:79597/97‑1 (MQ=255)
                                                                                                                                           cccACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCt                                                             >  2:79597/1‑97 (MQ=255)
                                                                                                                                           cccACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCgtgt         >  1:480965/1‑149 (MQ=255)
                                                                                                                                           cccACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCgtg          >  4:806643/1‑148 (MQ=255)
                                                                                                                                           accACGCGTTTCGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCgtg          >  4:608869/2‑148 (MQ=255)
                                                                                                                                            ccACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTc        >  1:865168/1‑149 (MQ=255)
                                                                                                                                              aCGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcga      >  1:779834/1‑149 (MQ=255)
                                                                                                                                              aCGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcga      >  1:263326/1‑149 (MQ=255)
                                                                                                                                                  gAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcgaccga  >  1:995360/1‑149 (MQ=255)
                                                                                                                                                   aaaCGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCGGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcgaccga  >  3:222278/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTCGACCGA  >  NC_000913/4315290‑4315584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: