Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,376,280 G→A 5.8% intergenic (+15/‑37) efp → / → ecnA polyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,376,2800GA 5.8% 451.5 / 11.8 137intergenic (+15/‑37)efp/ecnApolyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B
Reads supporting (aligned to +/- strand):  ref base G (59/70);  new base A (3/5);  total (62/75)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

Only 100 of 137 total aligned reads displayed.

CGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTC  >  NC_000913/4376136‑4376426
                                                                                                                                                |                                                                                                                                                  
cGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGcc                                                                                                                                                 <  4:149929/148‑1 (MQ=255)
   aGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGc                                                                                                                                              >  3:788647/1‑148 (MQ=255)
    ggTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAg                                                                                                                                            <  1:461658/149‑1 (MQ=255)
      tACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATGCGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAgg                                                                                                                                           <  3:142184/148‑1 (MQ=255)
       aCTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGc                                                                                                                                          <  3:827992/148‑1 (MQ=255)
       aCTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCt                                                                                                                                         <  1:643137/149‑1 (MQ=255)
          ggtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATATGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGc                                                                                                                                       <  3:571989/148‑1 (MQ=255)
               cAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                                                                 <  1:1029851/149‑1 (MQ=255)
                aaaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATc                                                                                                                                 <  3:414708/148‑1 (MQ=255)
                aaaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                <  1:7926/149‑1 (MQ=255)
                aaaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                >  1:280234/1‑149 (MQ=255)
                aaaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                >  2:248020/1‑149 (MQ=255)
                aaaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                >  1:377644/1‑149 (MQ=255)
                 aaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                >  3:473313/1‑148 (MQ=255)
                 aaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                >  4:669750/1‑148 (MQ=255)
                 aaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                <  3:411943/148‑1 (MQ=255)
                 aaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCa                                                                                                                                      >  3:229907/1‑142 (MQ=255)
                 aaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCACCacaac                                                                                                                               <  2:326945/149‑5 (MQ=255)
                 aaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCACCacaa                                                                                                                                <  2:260651/148‑4 (MQ=255)
                 aaCCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCACCacaa                                                                                                                                >  1:260651/1‑145 (MQ=255)
                         aCCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAg                                                                                                                       <  1:1009708/149‑1 (MQ=255)
                         aCCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAg                                                                                                                       <  1:724176/149‑1 (MQ=255)
                          cccTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAg                                                                                                                       <  4:473313/148‑1 (MQ=255)
                           ccTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAgg                                                                                                                      <  3:507140/148‑1 (MQ=255)
                           ccTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAgg                                                                                                                      <  4:422070/148‑1 (MQ=255)
                            cTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGt                                                                                                                     >  4:226406/1‑148 (MQ=255)
                             tGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCa                                                                                                                   <  1:143493/149‑1 (MQ=255)
                               tCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCaga                                                                                                                 <  1:248020/149‑1 (MQ=255)
                                cTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGTGGCCTGCAGGCTGCACCATCACTTATTCAGGTCaga                                                                                                                 <  4:624101/148‑1 (MQ=255)
                                         cTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGaaa                                                                                                       <  2:1008582/149‑1 (MQ=255)
                                              gTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCt                                                                                                  >  1:488805/1‑149 (MQ=255)
                                               ttAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGcc                                                                                                                                                 >  4:609718/1‑101 (MQ=255)
                                               ttAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGcc                                                                                                                                                 <  3:609718/101‑1 (MQ=255)
                                               ttAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCtt                                                                                                 >  1:361823/1‑149 (MQ=255)
                                               ttAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCt                                                                                                  <  4:770733/148‑1 (MQ=255)
                                                tAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTg                                                                                                                                        <  3:763881/109‑1 (MQ=255)
                                                tAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTg                                                                                                                                        >  4:763881/1‑109 (MQ=255)
                                                     ttCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGtt                                                                                           <  1:531249/149‑1 (MQ=255)
                                                     ttCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGtt                                                                                           <  1:402118/149‑1 (MQ=255)
                                                     ttCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGt                                                                                            <  3:993688/148‑1 (MQ=255)
                                                      tCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGtt                                                                                           <  4:322841/148‑1 (MQ=255)
                                                       ccGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTc                                                                                          >  4:44903/1‑148 (MQ=255)
                                                       ccGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTc                                                                                          <  4:788647/148‑1 (MQ=255)
                                                         gCTGTATGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCtt                                                                                                 >  4:3669/1‑139 (MQ=255)
                                                           tGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGt                                                                                      <  4:104485/148‑1 (MQ=255)
                                                             tttGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAg                                                                                                                       >  2:78416/1‑113 (MQ=255)
                                                             tttGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAg                                                                                                                       <  1:78416/113‑1 (MQ=255)
                                                              ttGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtttt                                                                                   >  3:330256/1‑148 (MQ=255)
                                                              ttGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtttt                                                                                   <  3:669750/148‑1 (MQ=255)
                                                               tGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttg                                                                                  <  3:386338/148‑1 (MQ=255)
                                                                 tACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgct                                                                                >  3:140204/1‑148 (MQ=255)
                                                                 tACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgct                                                                                <  3:239768/148‑1 (MQ=255)
                                                                  aCAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgctt                                                                               >  4:287386/1‑148 (MQ=255)
                                                                   cAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTAt                                                                                                                           <  3:364008/103‑1 (MQ=255)
                                                                   cAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttgc                                                                             <  2:711396/149‑1 (MQ=255)
                                                                   cAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttg                                                                              <  3:229892/148‑1 (MQ=255)
                                                                    aaaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTAtt                                                                                                                          >  1:57155/1‑103 (MQ=255)
                                                                    aaaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTAtt                                                                                                                          <  2:57155/103‑1 (MQ=255)
                                                                     aaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGcc                                                                            >  4:367030/1‑148 (MQ=255)
                                                                      aTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAg                                                                          >  2:844954/1‑149 (MQ=255)
                                                                            gAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATc                                                                                              <  1:330288/123‑1 (MQ=255)
                                                                            gAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATc                                                                                              >  2:330288/1‑123 (MQ=255)
                                                                            gAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgct                                                                    >  2:386097/1‑149 (MQ=255)
                                                                             aaGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGt                                                                                      <  4:752776/130‑1 (MQ=255)
                                                                             aaGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGt                                                                                      >  3:752776/1‑130 (MQ=255)
                                                                             aaGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgct                                                                    >  4:366668/1‑148 (MQ=255)
                                                                                tcatcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctgct                                                                 >  4:1000891/1‑148 (MQ=255)
                                                                                  atcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCAc                                                              >  1:153055/1‑149 (MQ=255)
                                                                                  atcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACCTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTGTGCTTGCCAGCACGCTGCTCAc                                                              >  1:923427/1‑149 (MQ=255)
                                                                                   tcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatg                                                                                                             <  4:119005/101‑1 (MQ=255)
                                                                                   tcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatg                                                                                                             >  3:119005/1‑101 (MQ=255)
                                                                                   tcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCAc                                                              >  3:583032/1‑148 (MQ=255)
                                                                                   tcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACGTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACg                                                             >  1:310745/1‑149 (MQ=255)
                                                                                    caAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACgg                                                            <  1:1056859/149‑1 (MQ=255)
                                                                                    caAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACg                                                             <  3:821185/148‑1 (MQ=255)
                                                                                      aaGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgctt                                                                               >  1:1026342/1‑128 (MQ=255)
                                                                                      aaGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgctt                                                                               <  2:1026342/128‑1 (MQ=255)
                                                                                        gTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTa                                                                                                                            <  2:828924/81‑1 (MQ=255)
                                                                                        gTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgct                                                                                >  1:575409/1‑125 (MQ=255)
                                                                                        gTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgct                                                                                <  2:575409/125‑1 (MQ=255)
                                                                                             tACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAaca                                                   >  1:493044/1‑149 (MQ=255)
                                                                                                ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCAc                                                                                                                               <  2:1041230/70‑1 (MQ=255)
                                                                                                ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACAcc                                                 >  4:1058551/1‑148 (MQ=255)
                                                                                                 cGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCg                                                >  3:891410/1‑148 (MQ=255)
                                                                                                          gAATACGTCTCTCGCGTGAAGTCATGCGGTTGTGGTGCGGCCTGCAGGCTGCAACATCACTTATTCAGATCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTCCCAGCACGCTGCTCACGGGCTGTAACATCGCTCGGGGtt                                       >  4:884426/1‑148 (MQ=255)
                                                                                                             tACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCgg                                   <  2:220429/149‑1 (MQ=255)
                                                                                                              aCGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCgg                                   >  3:466403/1‑148 (MQ=255)
                                                                                                                 tctctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGaa                               <  2:377644/149‑1 (MQ=255)
                                                                                                                 tctctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGaa                               <  1:690537/149‑1 (MQ=255)
                                                                                                                   tctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGa                             >  2:446621/1‑149 (MQ=255)
                                                                                                                   tctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGa                             <  2:855777/149‑1 (MQ=255)
                                                                                                                          tGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCaaa                      <  1:607805/149‑1 (MQ=255)
                                                                                                                          tGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCaa                       <  3:671636/148‑1 (MQ=255)
                                                                                                                               tAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACAtct                 >  1:449955/1‑149 (MQ=255)
                                                                                                                                   gCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCgg              >  4:144401/1‑148 (MQ=255)
                                                                                                                                   gCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGc             <  1:386097/149‑1 (MQ=255)
                                                                                                                                     ggTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCaa           >  1:84351/1‑149 (MQ=255)
                                                                                                                                           ggtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCa      <  4:460726/148‑1 (MQ=255)
                                                                                                                                            gtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCAt     <  4:466403/148‑1 (MQ=255)
                                                                                                                                              gCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCAtctc  <  2:493044/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTC  >  NC_000913/4376136‑4376426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: