Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 533,846 T→C 6.7% intergenic (+20/‑70) allR → / → gcl glyoxylate‑inducible transcriptional repressor of all and gcl operons/glyoxylate carboligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913533,8460TC 6.7% 533.9 / 18.9 164intergenic (+20/‑70)allR/gclglyoxylate‑inducible transcriptional repressor of all and gcl operons/glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base T (79/74);  new base C (5/6);  total (84/80)
Fisher's exact test for biased strand distribution p-value = 7.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 166 total aligned reads displayed.

GCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGG  >  NC_000913/533704‑533994
                                                                                                                                              |                                                                                                                                                     
gCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAg                                                                                                                                                 >  1:559954/1‑149 (MQ=255)
 cTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGc                                                                                                                                                <  2:607058/149‑1 (MQ=255)
    tctccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCggg                                                                                                                                             <  1:489827/149‑1 (MQ=255)
    tctccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCCGCAGAGCggg                                                                                                                                             <  1:191231/149‑1 (MQ=255)
     ctccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCCGCAGAGCggga                                                                                                                                            <  1:919311/149‑2 (MQ=255)
      tccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCCGCAGAGCggga                                                                                                                                            <  3:886746/148‑2 (MQ=255)
       ccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGtt                                                                                                                                           >  3:896777/1‑148 (MQ=255)
       ccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCCGCAGAGCgggat                                                                                                                                           <  2:743765/148‑3 (MQ=255)
       ccatctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCCGCAGAGCgggat                                                                                                                                           >  1:743765/1‑146 (MQ=255)
           ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGttttttt                                                                                                                                      <  1:728440/149‑1 (MQ=255)
           ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGtttttt                                                                                                                                       <  3:193373/148‑1 (MQ=255)
           ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGtttttt                                                                                                                                       <  3:222942/148‑1 (MQ=255)
           ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGtttttt                                                                                                                                       <  3:490593/148‑1 (MQ=255)
           ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGtttttt                                                                                                                                       <  3:518149/148‑1 (MQ=255)
           ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGtttttt                                                                                                                                       <  3:542917/148‑1 (MQ=255)
           ctccGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGtttttt                                                                                                                                       <  3:1005094/148‑1 (MQ=255)
                  ccTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACaaaa                                                                                                                               <  2:559954/149‑1 (MQ=255)
                    ttCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAAt                                                                                                                              >  3:583551/1‑148 (MQ=255)
                     tcatcaAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAAtt                                                                                                                             >  4:552716/1‑148 (MQ=255)
                         caAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGATCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGaa                                                                                                                         >  4:872164/1‑148 (MQ=255)
                            gACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGtt                                                                                                                     <  1:1029339/149‑1 (MQ=255)
                             aCTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGtt                                                                                                                     <  4:105301/148‑1 (MQ=255)
                                gACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGa                                                                                                                 >  4:593846/1‑148 (MQ=255)
                                  cAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGaaa                                                                                                               >  3:564168/1‑148 (MQ=255)
                                        aTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTc                                                                                                        >  2:1055180/1‑149 (MQ=255)
                                        aTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTc                                                                                                        >  1:681701/1‑149 (MQ=255)
                                             tttGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCaat                                                                                                    >  4:481058/1‑148 (MQ=255)
                                             tttGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCaat                                                                                                    >  3:685183/1‑148 (MQ=255)
                                                 tcagtcagGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCaataaata                                                                                               <  2:978109/149‑1 (MQ=255)
                                                   agtcagGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATaga                                                                                             >  2:384573/1‑149 (MQ=255)
                                                    gtcagGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATaga                                                                                             <  4:138592/148‑1 (MQ=255)
                                                    gtcagGGTGAGATGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAATCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATagag                                                                                            >  1:877168/1‑149 (MQ=255)
                                                     tcagGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAgg                                                                                           >  1:471769/1‑149 (MQ=255)
                                                     cccgggTGAGCTGGTCAGAGACACCGCAAGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCCTAATGTCTGTCCCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAgg                                                                                           >  2:293595/4‑149 (MQ=255)
                                                        gggTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAg                                                                                        <  2:112508/149‑1 (MQ=255)
                                                        gggTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAg                                                                                        <  2:19068/149‑1 (MQ=255)
                                                            gAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCATATCCATAATGTCTGTCGCATCCCGCTCTTCAGAGCGGGTTTTTTTGACAAAATTTGAAAGTTTGAAAAATTTTCCAATAAATAGAGGTAGGaa                                                                                     <  4:456112/148‑1 (MQ=255)
                                                            gAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGAGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAAt                                                                                    <  2:756048/149‑1 (MQ=255)
                                                                tGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCCGCAGAGCggg                                                                                                                                             >  2:944509/1‑89 (MQ=255)
                                                                  gTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATg                                                                              <  2:681701/149‑1 (MQ=255)
                                                                   tCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTGGGAATAAAATgg                                                                             >  1:45306/1‑149 (MQ=255)
                                                                     agagACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCa                                                                           >  1:873927/1‑149 (MQ=255)
                                                                      gagaCACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAAtt                                                                                                                             <  3:451868/99‑1 (MQ=255)
                                                                      gagaCACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCa                                                                           <  4:105295/148‑1 (MQ=255)
                                                                      gagaCACCGCCCGCGATATCAGCACGGCGCTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCaa                                                                          >  1:650608/1‑149 (MQ=255)
                                                                       agaCACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCaataaat                                                                                                >  4:891823/1‑126 (MQ=255)
                                                                         acacCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCaaaa                                                                        >  4:606218/1‑148 (MQ=255)
                                                                            ccgcccgcGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTa                                                                                         >  3:159580/1‑128 (MQ=255)
                                                                            ccgcccgcGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTa                                                                                         <  4:159580/128‑1 (MQ=255)
                                                                            ccgcccgcGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATga                                                                    <  2:1044391/149‑1 (MQ=255)
                                                                            ccgcccgcGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATg                                                                     <  3:606218/148‑1 (MQ=255)
                                                                            ccgcccgcGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATg                                                                     <  4:694993/148‑1 (MQ=255)
                                                                             cgcccgcGATATCAGCACGGCGTTGGGACTGAAAGCACCTCCATAATGTCTGTCACATCCCGCTCTGCGGAGCGGGTTTTGCTGACAATATTTGAAAGTTGGAAAAATTTTCAAAAAAATAGAGGTAGGAATAAAATGGATAAAATgag                                                                   >  2:88063/1‑149 (MQ=255)
                                                                              gcccgcGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATgaga                                                                  <  2:1058944/149‑1 (MQ=255)
                                                                                   cgATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGt                                                                                                                                            <  4:933941/71‑1 (MQ=255)
                                                                                   cgATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGt                                                             <  2:877168/149‑1 (MQ=255)
                                                                                       atCAGCACGGCGNTGGGACTGAAAGCACNTCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTTCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGATCCGTGGAc                                                         >  2:303080/1‑149 (MQ=255)
                                                                                         cAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGAcgc                                                       <  2:45306/149‑1 (MQ=255)
                                                                                                  gTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAatgta                                              >  1:95656/1‑149 (MQ=255)
                                                                                                    tGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAatgtat                                             <  4:627211/148‑1 (MQ=255)
                                                                                                     gggACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAGATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAatgtatgt                                           >  1:948674/1‑149 (MQ=255)
                                                                                                     gggACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAatgtatgt                                           <  1:246095/149‑1 (MQ=255)
                                                                                                      ggACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAatgtatgt                                           >  4:579737/1‑148 (MQ=255)
                                                                                                       gACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTAtgtg                                          >  4:448579/1‑148 (MQ=255)
                                                                                                       gACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTAtgtg                                          <  4:2705/148‑1 (MQ=255)
                                                                                                       gACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGc                                         >  2:809284/1‑149 (MQ=255)
                                                                                                         cTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGATAGTTGGAAAAATTTTCCATGAAATAGAGGTAGGAATAAAATGGCAAAACTGAGAGCCGTTGACGCGGCAatgtattttct                                        <  4:671727/148‑6 (MQ=255)
                                                                                                         cTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTg                                       <  1:1055180/149‑1 (MQ=255)
                                                                                                          tGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAAttt                                                                                                                            >  2:295273/1‑64 (MQ=255)
                                                                                                          tGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCgg                                                     <  1:687860/134‑1 (MQ=255)
                                                                                                          tGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCgg                                                     >  2:687860/1‑134 (MQ=255)
                                                                                                            aaaGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTgg                                      <  4:492620/148‑1 (MQ=255)
                                                                                                              aGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGagaa                                  >  1:565510/1‑149 (MQ=255)
                                                                                                               gCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGagaaa                                 >  1:327159/1‑149 (MQ=255)
                                                                                                                cacaTCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGagaa                                  >  4:612712/1‑147 (MQ=255)
                                                                                                                   aTCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGagaaagaa                              <  3:953236/148‑1 (MQ=255)
                                                                                                                   aTCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAaagaag                             <  1:384573/149‑1 (MQ=255)
                                                                                                                    tCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAAt                                                                               <  3:36391/98‑1 (MQ=255)
                                                                                                                    tCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAAt                                                                               >  4:36391/1‑98 (MQ=255)
                                                                                                                    tCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCaa                                                  >  3:447451/1‑127 (MQ=255)
                                                                                                                    tCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAgg                            >  1:11091/1‑149 (MQ=255)
                                                                                                                    tCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAgg                            >  2:446761/1‑149 (MQ=255)
                                                                                                                     ccATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGttatgg                                                        >  3:667355/1‑116 (MQ=255)
                                                                                                                     ccATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATCTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGttatgg                                                        <  4:667355/120‑5 (MQ=255)
                                                                                                                      cATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGt                           >  3:221151/1‑148 (MQ=255)
                                                                                                                       aTAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGtt                                                                                                                     >  4:338692/1‑58 (MQ=255)
                                                                                                                       aTAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTAGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTa                          >  3:690184/1‑148 (MQ=255)
                                                                                                                        tAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGc                                                                >  3:142743/1‑109 (MQ=255)
                                                                                                                                 tcGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGcc               >  2:185573/1‑149 (MQ=255)
                                                                                                                                 tcGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGcc               >  1:842434/1‑149 (MQ=255)
                                                                                                                                 tcGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGcc               >  1:307614/1‑149 (MQ=255)
                                                                                                                                  aaaaacccGCTCCGCAGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGGAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCt              >  2:542633/6‑149 (MQ=255)
                                                                                                                                    cATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCtt             >  4:18989/1‑148 (MQ=255)
                                                                                                                                     aTCCCGCCCTGCGGAGCGGGTTTTTTTGACAAAATTTTAAAGTTGGAAAAATTTTCCAATAAATAGAGGTTGGAATTAAATGGCAAAAATGAGAGCCGTTGAAGCCGCAATGTTATTTCTGGAGGAAGAAGGTATTACTACCGCCTTCg           <  2:732143/149‑1 (MQ=255)
                                                                                                                                       cccGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCgg          >  3:896829/1‑148 (MQ=255)
                                                                                                                                        ccGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGgtg        >  2:555179/1‑149 (MQ=255)
                                                                                                                                         cGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATG‑‑TGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTc     >  1:124900/1‑149 (MQ=255)
                                                                                                                                          gCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGtt      >  1:80125/1‑149 (MQ=255)
                                                                                                                                              tGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCgg  <  1:294745/149‑1 (MQ=255)
                                                                                                                                              tGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCgg  <  1:158273/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
GCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGG  >  NC_000913/533704‑533994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: