Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 604,752 G→T 10.0% intergenic (‑89/+19) ybdG ← / ← nfsB mechanosensitive channel protein, miniconductance/dihydropteridine reductase, NAD(P)H‑dependent, oxygen‑insensitive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913604,7520GT10.0% 369.4 / 26.0 110intergenic (‑89/+19)ybdG/nfsBmechanosensitive channel protein, miniconductance/dihydropteridine reductase, NAD(P)H‑dependent, oxygen‑insensitive
Reads supporting (aligned to +/- strand):  ref base G (46/53);  new base T (7/4);  total (53/57)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01

Only 100 of 111 total aligned reads displayed.

GTGGTGTGATCGATCTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTCAGAC  >  NC_000913/604605‑604895
                                                                                                                                                   |                                                                                                                                               
   gtgtGATCGATCTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGc                                                                                                                                             <  1:467040/149‑1 (MQ=255)
     gtgATCGATCTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGcc                                                                                                                                            <  4:140044/148‑1 (MQ=255)
        atcgatcTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCATCTGCCCGGc                                                                                                                                        <  1:672579/149‑1 (MQ=255)
        atcgatcTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCATCTGCCCAGc                                                                                                                                        >  1:1045918/1‑149 (MQ=255)
        atcgatcTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGc                                                                                                                                        >  2:632848/1‑149 (MQ=255)
        atcgatcTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGc                                                                                                                                        >  2:733670/1‑149 (MQ=255)
          cgatcTCAATACACGCTAAGTCTGCAATCTGGGATAGGAAATCCTGCATAACGTCTCCTTTATAGAACAGCAGCCTATGACGGCGGCTGAAACATGGTGATGCAGATCAGGAGAAGCTGAGAGGCAATAGCCGAGCAGATGCCCGACa                                                                                                                                       >  4:88048/1‑148 (MQ=255)
            atcTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAaga                                                                                                                                    <  1:209404/149‑1 (MQ=255)
            atcTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAag                                                                                                                                     <  4:815871/148‑1 (MQ=255)
               tcAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAagagag                                                                                                                                 >  1:917564/1‑149 (MQ=255)
                 aaTACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAgagaga                                                                                                                                <  4:472154/148‑1 (MQ=255)
                 aaTACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGaa                                                                                                                               <  1:436585/149‑1 (MQ=255)
                  aTACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGaa                                                                                                                               >  4:860306/1‑148 (MQ=255)
                      ccGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTaca                                                                                                                          >  2:748414/1‑149 (MQ=255)
                       cGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTaca                                                                                                                          <  3:536450/148‑1 (MQ=255)
                         cTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTacacat                                                                                                                       >  1:89128/1‑147 (MQ=255)
                          tAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACtt                                                                                                                       >  4:706367/1‑148 (MQ=255)
                            aaTCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAATAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACCTCgg                                                                                                                    >  1:691801/1‑149 (MQ=255)
                                 tCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTaa                                                                                                                <  4:188147/148‑1 (MQ=255)
                                 tCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTaa                                                                                                                <  3:923600/148‑1 (MQ=255)
                                 tCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAg                                                                                                               <  2:1001556/149‑1 (MQ=255)
                                  cAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAgg                                                                                                              >  1:206971/1‑149 (MQ=255)
                                     ccTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTg                                                                                                            <  3:860306/148‑1 (MQ=255)
                                       tGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGAt                                                                                                          >  3:28502/1‑148 (MQ=255)
                                        gggATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGt                                                                                                        <  2:503930/149‑1 (MQ=255)
                                             atTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGc                                                                                                   <  2:258657/149‑1 (MQ=255)
                                              ttAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCg                                                                                                  >  2:945993/1‑149 (MQ=255)
                                              ttAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCg                                                                                                  >  2:156898/1‑149 (MQ=255)
                                                 aaTCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGc                                                                                                <  4:390806/148‑1 (MQ=255)
                                                  aTCCTTCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAg                                                                                                               <  1:742761/132‑1 (MQ=255)
                                                  aTCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAg                                                                                                               >  2:742761/1‑132 (MQ=255)
                                                  aTCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCa                                                                                               >  4:384212/1‑148 (MQ=255)
                                                   tCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAg                                                                                              <  3:632547/148‑1 (MQ=255)
                                                     cTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACg                                                                                           >  1:1046807/1‑149 (MQ=255)
                                                       gCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACgag                                                                                         >  1:189158/1‑149 (MQ=255)
                                                        cATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACgag                                                                                         <  3:443423/148‑1 (MQ=255)
                                                          tAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGaa                                                                                                                               >  1:512654/1‑108 (MQ=255)
                                                            aCGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGAttt                                                                                     >  3:127083/1‑148 (MQ=255)
                                                            aCGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGAttt                                                                                     >  3:195202/1‑148 (MQ=255)
                                                            aCGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGAttt                                                                                     >  3:942428/1‑148 (MQ=255)
                                                            aCGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTc                                                                                    <  2:688890/149‑1 (MQ=255)
                                                                ctcCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGc                                                                                 <  3:982961/148‑1 (MQ=255)
                                                                 tcCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCag                                                                               >  1:138981/1‑149 (MQ=255)
                                                                 tcCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCag                                                                               >  2:55238/1‑149 (MQ=255)
                                                                  ccTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCag                                                                               >  3:440408/1‑148 (MQ=255)
                                                                    tttATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTac                                                                                                                           >  4:771102/1‑102 (MQ=255)
                                                                    tttATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTac                                                                                                                           <  3:771102/102‑1 (MQ=255)
                                                                     ttATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCagcgta                                                                           <  1:55238/149‑1 (MQ=255)
                                                                        taCAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCagcgtagc                                                                         <  3:640862/148‑1 (MQ=255)
                                                                         acaacaGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCagcgtagcgt                                                                       >  1:58597/1‑149 (MQ=255)
                                                                          caacaGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCagcgtagcgt                                                                       <  3:139581/148‑1 (MQ=255)
                                                                           aacaGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCagcgtagc                                                                         <  1:481646/145‑1 (MQ=255)
                                                                           aacaGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCagcgtagc                                                                         >  2:481646/1‑145 (MQ=255)
                                                                            acaGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTa                                                                     <  3:435740/148‑1 (MQ=255)
                                                                              agcagcCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTaaaa                                                                  >  1:917379/1‑149 (MQ=255)
                                                                                  gcCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCtt                                                              <  2:917379/149‑1 (MQ=255)
                                                                                   ccTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTc                                                             >  1:153188/1‑149 (MQ=255)
                                                                                     tATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCagcgtag                                                                          <  3:760824/134‑1 (MQ=255)
                                                                                     tATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCagcgtag                                                                          >  4:760824/1‑134 (MQ=255)
                                                                                     tATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCa                                                            >  3:1026062/1‑148 (MQ=255)
                                                                                     tATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCa                                                            >  4:339919/1‑148 (MQ=255)
                                                                                      atgatgACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAAc                                                          <  2:691801/149‑1 (MQ=255)
                                                                                      atgatgACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAAc                                                          >  1:390807/1‑149 (MQ=255)
                                                                                       tgatgaCGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACg                                                         <  2:917564/149‑1 (MQ=255)
                                                                                       tgatgaCGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCCGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACg                                                         >  1:152505/1‑149 (MQ=255)
                                                                                           gaCGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCtgt                                                     <  1:733670/149‑1 (MQ=255)
                                                                                              ggCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGtgat                                                  <  2:73916/149‑1 (MQ=255)
                                                                                                        gggTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCg                                         >  3:809320/1‑148 (MQ=255)
                                                                                                          gTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGaa                                      >  1:717363/1‑149 (MQ=255)
                                                                                                          gTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGa                                       >  4:908580/1‑148 (MQ=255)
                                                                                                              tGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCTGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGaacaa                                   <  3:706367/148‑1 (MQ=255)
                                                                                                              tGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGaacaac                                  <  1:1044200/149‑1 (MQ=255)
                                                                                                               gCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGaacaac                                  <  3:384212/148‑1 (MQ=255)
                                                                                                                 aaaTCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAacca                                <  3:368521/148‑1 (MQ=255)
                                                                                                                        gagaATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTg                        <  1:156898/149‑1 (MQ=255)
                                                                                                                        gagaATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTg                        <  2:58597/149‑1 (MQ=255)
                                                                                                                           aaTCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGgtg                     <  2:206971/149‑1 (MQ=255)
                                                                                                                           aaTCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGgt                      >  4:331903/1‑148 (MQ=255)
                                                                                                                             tCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTa                   >  1:758844/1‑149 (MQ=255)
                                                                                                                               ttagagGAAATAGCCGGGCAGATGCCCGGCTAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGTCTGgtggtt                  <  3:862514/146‑4 (MQ=255)
                                                                                                                                gagaGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGc                 >  3:995554/1‑148 (MQ=255)
                                                                                                                                    ggAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCttt             <  4:195202/148‑1 (MQ=255)
                                                                                                                                    ggAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCttt             <  3:339919/148‑1 (MQ=255)
                                                                                                                                    ggAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTtc            >  2:387780/1‑149 (MQ=255)
                                                                                                                                      ctcttgCCGGGCATCTGCCCGGCTAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTa                                            >  4:888004/6‑115 (MQ=255)
                                                                                                                                      ctcttgCCGGGCATCTGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTtct           >  4:601366/6‑148 (MQ=255)
                                                                                                                                      aaaTAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGaacaa                                   >  3:276173/1‑124 (MQ=255)
                                                                                                                                      aaaTAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTtct           >  4:315442/1‑148 (MQ=255)
                                                                                                                                      aaaTAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTtct           >  4:499570/1‑148 (MQ=255)
                                                                                                                                       tcttgCCGGGCATCTGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTctct         >  2:890677/5‑149 (MQ=255)
                                                                                                                                         ttgCCGGGCATCTGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCttt       >  1:330210/3‑149 (MQ=255)
                                                                                                                                         tAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCtt        <  4:926847/148‑1 (MQ=255)
                                                                                                                                          tgCCGGGCATCTGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCtgt                                                     >  4:149721/2‑102 (MQ=255)
                                                                                                                                          tgCCGGGCATCTGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCtgt                                                     <  3:149721/101‑1 (MQ=255)
                                                                                                                                          aGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTc      <  2:390807/149‑1 (MQ=255)
                                                                                                                                           gCCGGGCATCTGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTCa     <  1:770369/149‑1 (MQ=255)
                                                                                                                                           gCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTc      <  4:1026062/148‑1 (MQ=255)
                                                                                                                                             cGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTCAg    <  4:28502/148‑1 (MQ=255)
                                                                                                                                             cGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTCAGa   <  2:138981/149‑1 (MQ=255)
                                                                                                                                               ggCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTCAGAc  >  3:812489/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GTGGTGTGATCGATCTCAATACCCGCTAAATCTTCAACCTGGGATATTAAATCCTGCATAACGTCTCCTTTATACAACAGCAGCCTATGATGACGGCTGAAACAGGGTTATGCAAATCAGGAGAATCTGAGAGGAAATAGCCGGGCAGATGCCCGGCAAGAGAGAATTACACTTCGGTTAAGGTGATGTTTTGCGGCAGACGAGATTTCGGCAGCGTAGCGTTAAAATCTTCAACGCTGTGATGACCTACCGGAACAACCACCAGACTGGTGTAGCCTTTCTCTTTCAGAC  >  NC_000913/604605‑604895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: