Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,350,678 | Δ1 bp | 5.1% | noncoding (102/121 nt) | arcZ → | sRNA positive antisense regulator of rpoS; binds Hfq |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,350,678 | 0 | T | . | 5.1% | 557.8 / 20.1 | 158 | noncoding (102/121 nt) | arcZ | sRNA positive antisense regulator of rpoS; binds Hfq |
Reads supporting (aligned to +/- strand): ref base T (71/79); new base . (2/6); total (73/85) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.88e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.07e-02 |
Only 100 of 161 total aligned reads displayed.
TAACTGATTCATGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGA > NC_000913/3350530‑3350821 | tAACTGATTCATGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGt < 3:232131/149‑1 (MQ=255) aaCTGATTCATGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTc < 3:365559/149‑1 (MQ=255) acagATTCATGTAACAAATCATATATGTTTTGCTATCTTAACTGCGTGCGGTCTGAAAAACAGTGCTGTGCACTTGTAACTCATCATAATAATTTACGGCGCAGCAAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCt > 4:977288/4‑149 (MQ=255) ttCATGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCg > 4:731693/1‑149 (MQ=255) ttCATGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCg > 2:1085977/1‑149 (MQ=255) tGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGG‑CTAGACCGGGGt < 2:900731/149‑1 (MQ=255) tGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGG‑CTAGACCGGGGt < 4:138999/149‑1 (MQ=255) tGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGG‑CTAGACCGGGGt < 3:640312/149‑1 (MQ=255) gTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTc > 1:161984/1‑149 (MQ=255) aCAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGG‑CTAGACCGGGGt > 4:502250/1‑145 (MQ=255) aCAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGG‑CTAGACCGGGGt < 2:1474133/145‑1 (MQ=255) aCAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGG‑CTAGACCGGGGt > 1:1474133/1‑145 (MQ=255) aaTCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCAttttt > 1:701267/1‑149 (MQ=255) aaTCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCAttttt > 2:1568018/1‑149 (MQ=255) aaTCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCAttttt > 4:826470/1‑149 (MQ=255) aaTCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCAttttt > 2:328439/1‑149 (MQ=255) tCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTa < 1:1461683/149‑1 (MQ=255) tCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTa < 2:157892/149‑1 (MQ=255) tCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTa < 3:175158/149‑1 (MQ=255) tCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTa > 2:95841/1‑149 (MQ=255) tCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTa < 4:149463/149‑1 (MQ=255) aTTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGt > 3:396849/1‑149 (MQ=255) aTTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGt > 2:809691/1‑149 (MQ=255) tttAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCgggg > 3:100167/1‑136 (MQ=255) tttAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTg > 1:1332373/1‑149 (MQ=255) aGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCgg < 2:444849/130‑1 (MQ=255) aGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCgg > 1:444849/1‑130 (MQ=255) aGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCtt < 1:658353/149‑1 (MQ=255) gTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCttt > 1:1418979/1‑149 (MQ=255) ttttGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCtttt < 1:1290484/149‑1 (MQ=255) tttGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTg < 3:1477747/149‑1 (MQ=255) aTCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGccaccc < 3:932435/149‑1 (MQ=255) cTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGccacccac > 2:511291/1‑149 (MQ=255) cTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGccacccac < 3:469341/149‑1 (MQ=255) tAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGc > 1:736541/1‑149 (MQ=255) cTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCttt < 2:604640/149‑1 (MQ=255) cTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCACCATAATAATTTACGGCGCAGCCAAGATTTCTCTGTTGTTGGCGCAGTATTCCCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCttt > 3:818845/1‑149 (MQ=255) tgcgtgcgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGccaccca > 1:599651/1‑142 (MQ=255) gcgtgcgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCa < 2:1366000/149‑1 (MQ=255) gcgtgcgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCa < 3:1132560/149‑1 (MQ=255) ccTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGt > 1:545340/1‑96 (MQ=255) ccTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGt < 2:545340/96‑1 (MQ=255) tGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAc > 4:1329771/1‑149 (MQ=255) tGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAc > 3:1267462/1‑149 (MQ=255) gAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcg > 2:1251027/1‑149 (MQ=255) gAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcg > 3:205546/1‑149 (MQ=255) aaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCtttt > 1:913312/1‑118 (MQ=255) aaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcgtcgt < 1:1108104/149‑1 (MQ=255) cactGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGCGa < 3:1386739/146‑1 (MQ=255) cTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCa > 3:868439/1‑149 (MQ=255) tgtgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCAc > 1:458790/1‑149 (MQ=255) cTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACtcttct < 2:1419841/149‑1 (MQ=255) ttGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACtcttct < 1:272057/148‑1 (MQ=255) gTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTc < 1:1217886/149‑1 (MQ=255) gTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTc < 2:182839/149‑1 (MQ=255) gTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCATGACGCCACTCTTCTTTc < 4:1121676/149‑1 (MQ=255) tAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCa < 1:266058/149‑1 (MQ=255) aaCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCAt < 1:368948/84‑1 (MQ=255) aaCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCAt > 3:909172/1‑149 (MQ=255) atcatAATAATTTACGGCTCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCtt > 1:1374271/1‑149 (MQ=255) atcatAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCtt > 1:26011/1‑149 (MQ=255) atcatAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCtt < 1:1085977/149‑1 (MQ=255) tcatAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCtt < 4:498824/148‑1 (MQ=255) tcatAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCtt > 3:498824/1‑148 (MQ=255) ataataATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCaa > 4:1022056/1‑149 (MQ=255) taataaTTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAAt > 3:5083/1‑149 (MQ=255) aataatTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTcttctt < 2:625394/135‑1 (MQ=255) aataatTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTcttctt > 1:625394/1‑135 (MQ=255) aataatTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATc > 2:402002/1‑149 (MQ=255) ataatTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATcc > 4:439797/1‑149 (MQ=255) tttACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTc < 3:1329771/149‑1 (MQ=255) aCGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCAcc < 2:1418979/149‑1 (MQ=255) gcgcAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGAc < 4:193280/149‑1 (MQ=255) aGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTa < 4:1277193/149‑1 (MQ=255) gCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTAt < 1:502735/149‑1 (MQ=255) ccAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATc > 3:1426725/1‑149 (MQ=255) cAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCtt < 4:63049/126‑1 (MQ=255) cAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTc < 4:814263/136‑1 (MQ=255) cAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTc > 3:814263/1‑136 (MQ=255) cAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCt > 3:403406/1‑149 (MQ=255) aaGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAg > 4:670380/1‑87 (MQ=255) aaGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAg < 3:670380/87‑1 (MQ=255) aaGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCtt > 2:112452/1‑149 (MQ=255) ttGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGt < 1:402002/149‑1 (MQ=255) ttGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGt > 1:1371329/1‑149 (MQ=255) ggCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCa < 4:396849/149‑1 (MQ=255) ggCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCa < 2:1309455/149‑1 (MQ=255) gcgcAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGa > 1:614646/1‑85 (MQ=255) gcgcAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGa < 2:614646/85‑1 (MQ=255) gTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGAc < 1:913661/149‑1 (MQ=255) aTTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTg > 2:924158/1‑149 (MQ=255) ttCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGa > 1:1224618/1‑149 (MQ=255) gcgcACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAAtt < 4:1426725/149‑1 (MQ=255) gcgcACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAAtt > 4:1261285/1‑149 (MQ=255) gcgcACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAAtt > 2:744559/1‑149 (MQ=255) cACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGc < 2:194713/149‑1 (MQ=255) cACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGc < 1:1568018/149‑1 (MQ=255) cACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGc < 1:1251027/149‑1 (MQ=255) cccGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGa > 2:1180975/1‑149 (MQ=255) cccGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGa > 1:400137/1‑149 (MQ=255) | TAACTGATTCATGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAG‑CCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGA > NC_000913/3350530‑3350821 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |