Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 120,158:1 +A 7.2% intergenic (+23/+20) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913120,1581.A 7.2% 612.0 / 46.2 152intergenic (+23/+20)ampE/aroPampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
Reads supporting (aligned to +/- strand):  ref base . (66/75);  new base A (9/2);  total (75/77)
Fisher's exact test for biased strand distribution p-value = 3.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.14e-01

Only 100 of 158 total aligned reads displayed.

TGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTAC  >  NC_000913/120011‑120306
                                                                                                                                                    |                                                                                                                                                    
 ggCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACgg                                                                                                                                                    <  3:146726/149‑1 (MQ=255)
 ggCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACgg                                                                                                                                                    <  3:1347545/149‑1 (MQ=255)
   cgcgTGAACCTCATGTCGATAAGGTGGAGACGACGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGt                                                                                                                                                  <  3:415754/149‑1 (MQ=255)
      gTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAg                                                                                                                                               <  3:603982/149‑1 (MQ=255)
       tGAACCGCATGTCGATAAGGTGGAGACGCGGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTAGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAgg                                                                                                                                              <  1:633353/149‑1 (MQ=255)
        gAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAggg                                                                                                                                             >  4:369729/1‑149 (MQ=255)
        gAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                                                                             <  2:303752/149‑1 (MQ=255)
         aaCCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGc                                                                                                                                            <  2:1498606/149‑1 (MQ=255)
           ccGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGt                                                                                                                                          >  2:847005/1‑149 (MQ=255)
           ccGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGt                                                                                                                                          <  1:624147/149‑1 (MQ=255)
             gCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTag                                                                                                                                        >  4:5142/1‑149 (MQ=255)
              cATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTaga                                                                                                                                       <  2:740496/149‑1 (MQ=255)
                  tCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAgagaga                                                                                                                                   <  1:1214979/149‑1 (MQ=255)
                   cgttAAAGTGGAGACGCCGGAGGCAGCGGTTTCAATGGCGAAGAAAGCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCCACCTGTACGGGTGAGGGCGTAGAGAAAt                                                                                                                                  <  3:1044647/146‑1 (MQ=255)
                    gATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGAtt                                                                                                                                 >  4:1044647/1‑149 (MQ=255)
                       aaGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAAt                                                                                                                              <  2:1359621/149‑1 (MQ=255)
                       aaGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAAt                                                                                                                              <  2:21134/149‑1 (MQ=255)
                          gTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATCGCCCTCACCCTGTACGGGTGAg                                                                                                                                               <  2:301786/129‑1 (MQ=255)
                           tGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgcgc                                                                                                                          <  4:1313328/149‑1 (MQ=255)
                             gagaCGGCGAATGCAGCGGCGTAAATGGCGAAGACCAGCCCGTACGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGAGGAGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAGTGCGCtt                                                                                                                        >  2:997770/1‑149 (MQ=255)
                              agaCGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCttt                                                                                                                       >  4:334541/1‑149 (MQ=255)
                                    cGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGc                                                                                                                 >  4:392302/1‑149 (MQ=255)
                                     gAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCt                                                                                                                >  1:732081/1‑149 (MQ=255)
                                       aGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCttt                                                                                                              <  1:847005/149‑1 (MQ=255)
                                           aGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCg                                                                                                          >  1:1516915/1‑149 (MQ=255)
                                            gCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTAc                                                                                                                    >  4:1259173/1‑138 (MQ=255)
                                            gCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTAc                                                                                                                    <  3:1259173/138‑1 (MQ=255)
                                              ggTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGtt                                                                                                       <  4:1217970/149‑1 (MQ=255)
                                                 ttCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTACTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtc                                                                                                    <  2:1104725/149‑1 (MQ=255)
                                                   cAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtctc                                                                                                  <  3:897416/149‑1 (MQ=255)
                                                    aaTGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTctct                                                                                                 <  3:692087/149‑1 (MQ=255)
                                                         cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCAGCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTaaa                                                                                            <  4:906590/149‑1 (MQ=255)
                                                         cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTaaa                                                                                            <  3:322712/149‑1 (MQ=255)
                                                          gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAAc                                                                                           >  4:1491555/1‑149 (MQ=255)
                                                          gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGACCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTctct                                                                                                 >  2:1212152/1‑143 (MQ=255)
                                                           aagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACa                                                                                          <  4:947500/149‑1 (MQ=255)
                                                           aagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACa                                                                                          <  1:519083/149‑1 (MQ=255)
                                                            agaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAg                                                                                         >  1:200945/1‑149 (MQ=255)
                                                                aaCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAg                                                                                     >  4:1464819/1‑149 (MQ=255)
                                                                aaCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAg                                                                                     >  2:1138454/1‑149 (MQ=255)
                                                                aaCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATCGCCCGCACCCGTACAGGGTGAgg                                                                                                                                              >  3:1327424/1‑92 (MQ=255)
                                                                 aCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGc                                                                                    <  2:1225863/149‑1 (MQ=255)
                                                                   cTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCg                                                                                  >  4:1149239/1‑149 (MQ=255)
                                                                    tcgttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGa                                                                                 >  2:811384/1‑149 (MQ=255)
                                                                    tcgttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGa                                                                                 <  3:5142/149‑1 (MQ=255)
                                                                           tggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTaa                                                                          <  2:435245/149‑1 (MQ=255)
                                                                            ggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAac                                                                         >  3:268406/1‑149 (MQ=255)
                                                                             gtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAaca                                                                        <  1:483092/149‑1 (MQ=255)
                                                                              tcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAacac                                                                       >  4:1187914/1‑149 (MQ=255)
                                                                                 tggtggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGAt                                                                    <  3:1280454/149‑1 (MQ=255)
                                                                                         aTTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCACACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATa                                                            <  1:1101382/149‑1 (MQ=255)
                                                                                          ttGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATAc                                                           >  2:15876/1‑149 (MQ=255)
                                                                                            gCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCg                                                         <  4:1388248/149‑1 (MQ=255)
                                                                                             cACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCgg                                                        >  1:509192/1‑149 (MQ=255)
                                                                                              actactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCggg                                                       >  2:185183/1‑149 (MQ=255)
                                                                                                  ctGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtctc                                                                                                  <  2:1560778/102‑1 (MQ=255)
                                                                                                   tGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAg                                                  >  1:609563/1‑149 (MQ=255)
                                                                                                   tGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAg                                                  >  2:1311852/1‑149 (MQ=255)
                                                                                                   tGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAg                                                  <  1:15876/149‑1 (MQ=255)
                                                                                                   tGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAg                                                  >  4:333759/1‑149 (MQ=255)
                                                                                                   tGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAg                                                  >  4:763933/1‑149 (MQ=255)
                                                                                                    gACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAgg                                                 <  3:369729/149‑1 (MQ=255)
                                                                                                     aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAacac                                                                       >  1:1279995/1‑126 (MQ=255)
                                                                                                     aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAacac                                                                       <  2:1279995/126‑1 (MQ=255)
                                                                                                       gATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAcc                                                                             >  1:1024021/1‑118 (MQ=255)
                                                                                                       gATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGtat                                              >  3:642995/1‑149 (MQ=255)
                                                                                                         tttACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGAt                                                                                >  3:1146776/1‑113 (MQ=255)
                                                                                                         tttACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGAt                                                                                <  4:1146776/113‑1 (MQ=255)
                                                                                                         tttACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATAc                                            <  4:1403625/149‑1 (MQ=255)
                                                                                                              ggggCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGttt                                                                                                      >  3:1459446/1‑86 (MQ=255)
                                                                                                                ggCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATc                                     <  1:1145375/149‑1 (MQ=255)
                                                                                                                  cGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGc                                   <  3:763933/149‑1 (MQ=255)
                                                                                                                    ttGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCa                                 >  3:20330/1‑149 (MQ=255)
                                                                                                                        tgtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTaaa                                                                                            >  1:291488/1‑86 (MQ=255)
                                                                                                                         gtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCt                            <  3:1149239/149‑1 (MQ=255)
                                                                                                                          tAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTg                           <  3:1187914/149‑1 (MQ=255)
                                                                                                                          tAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTg                           >  4:1083998/1‑149 (MQ=255)
                                                                                                                           aaaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTgg                          <  2:1516915/149‑1 (MQ=255)
                                                                                                                            aaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTggg                         <  1:503092/149‑1 (MQ=255)
                                                                                                                            aaGATTATTGCCATCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTggg                         <  1:1535886/149‑1 (MQ=255)
                                                                                                                              gATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTg                           >  1:112563/1‑145 (MQ=255)
                                                                                                                              gATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTg                           <  2:112563/145‑1 (MQ=255)
                                                                                                                                 tattGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGtcat                    <  1:899260/149‑1 (MQ=255)
                                                                                                                                  attGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGtcatc                   >  4:1207995/1‑149 (MQ=255)
                                                                                                                                   ttGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGtcatca                  >  3:986960/1‑149 (MQ=255)
                                                                                                                                   ttGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGtcatca                  >  1:269500/1‑149 (MQ=255)
                                                                                                                                      cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCa               >  1:1423108/1‑149 (MQ=255)
                                                                                                                                       ccTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCAt              >  1:312728/1‑149 (MQ=255)
                                                                                                                                       ccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCAt              >  4:623387/1‑149 (MQ=255)
                                                                                                                                       ccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCAt              >  2:203781/1‑149 (MQ=255)
                                                                                                                                        cTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTgggg                        >  3:1089081/1‑138 (MQ=255)
                                                                                                                                        cTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGt                                                >  1:730790/1‑114 (MQ=255)
                                                                                                                                         tCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATaa            >  3:179907/1‑149 (MQ=255)
                                                                                                                                         tCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATaa            >  3:476922/1‑149 (MQ=255)
                                                                                                                                         tCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATaa            >  3:850197/1‑149 (MQ=255)
                                                                                                                                           aCCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAGCTAACACGATCAGCCATACCGGGATCAg                                                  <  3:1348026/109‑1 (MQ=255)
                                                                                                                                           aCCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATc          >  4:595534/1‑149 (MQ=255)
                                                                                                                                             ccTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCAc        >  4:1550220/1‑149 (MQ=255)
                                                                                                                                              cTGTACGGGTGAGGGCGTAGAGAGATTAGTGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCAcc       >  2:425595/1‑149 (MQ=255)
                                                                                                                                               tGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCa      <  3:1208710/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGCGCGTGAACCGCATGTCGATAAGGTGGAGACGCCGAAGGCAGCGGTTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTAC  >  NC_000913/120011‑120306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: